466 research outputs found
Dynamic myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue
Citation: Aranjuez, G., Burtscher, A., Sawant, K., Majumder, P., & McDonald, J. A. (2016). Dynamic myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue. Molecular Biology of the Cell, 27(12), 1898-1910. doi:10.1091/mbc.E15-10-0744Migrating cells need to overcome physical constraints from the local microenvironment to navigate their way through tissues. Cells that move collectively have the additional challenge of negotiating complex environments in vivo while maintaining cohesion of the group as a whole. The mechanisms by which collectives maintain a migratory morphology while resisting physical constraints from the surrounding tissue are poorly understood. Drosophila border cells represent a genetic model of collective migration within a cell-dense tissue. Border cells move as a cohesive group of 6-10 cells, traversing a network of large germ line-derived nurse cells within the ovary. Here we show that the border cell cluster is compact and round throughout their entire migration, a shape that is maintained despite the mechanical pressure imposed by the surrounding nurse cells. Nonmuscle myosin II (Myo-II) activity at the cluster periphery becomes elevated in response to increased constriction by nurse cells. Furthermore, the distinctive border cell collective morphology requires highly dynamic and localized enrichment of Myo-II. Thus, activated Myo-II promotes cortical tension at the outer edge of the migrating border cell cluster to resist compressive forces from nurse cells. We propose that dynamic actomyosin tension at the periphery of collectives facilitates their movement through restrictive tissues
Mortality in Recreational Mountain-Biking in the Austrian Alps: A Retrospective Study over 16 Years.
Despite recreational mountain-biking's growing popularity worldwide, the literature on mortality in this leisure sporting activity is scarce. Therefore, the aim of the present study was to investigate the characteristics of fatal accidents as well as resulting dead victims during recreational mountain-biking in the Austrian Alps over the past 16 years. For this purpose, a retrospective study based on Austrian institutional documentation from 2006 to 2021 was conducted. In total, 97 fatalities (1 woman) with a mean age of 55.6 ± 13.9 years were recorded by the Austrian Alpine Police. Of those, 54.6% died due to a non-traumatic (mostly cardio-vascular) and 41.2% due to a traumatic event. Mountain-bikers fatally accidented for non-traumatic reasons frequently belonged to older age classes (p = 0.05) and mostly (73.6%) died during the ascent, whereas traumatic events mainly (70.0%) happened during the descent (p < 0.001). Throughout the examined period, the absolute number of fatalities slightly increased, whereas the mortality index (proportion of deaths/accidented victims) did not (mean value: 1.34 ± 0.56%). Factors such as male sex in general, above average age and uphill riding for non-traumatic accidents, as well as downhill riding for traumatic events, seem to be associated with fatalities during recreational mountain-biking in the Austrian Alps. These results should be considered for future preventive strategies in recreational mountain-biking
Peachy Parallel Assignments (EduHPC 2018)
Peachy Parallel Assignments are a resource for instructors teaching parallel and distributed programming. These are high-quality assignments, previously tested in class, that are readily adoptable. This collection of assignments includes implementing a subset of OpenMP using pthreads, creating an animated fractal, image processing using histogram equalization, simulating a storm of high-energy particles, and solving the wave equation in a variety of settings. All of these come with sample assignment sheets and the necessary starter code.Departamento de Informática (Arquitectura y Tecnología de Computadores, Ciencias de la Computación e Inteligencia Artificial, Lenguajes y Sistemas Informáticos)Facilitar la inclusión de ejercicios prácticos de programación paralela en cursos de Computación Paralela o de alto rendimiento (HPC)Comunicación en congreso: Descripción de ejercicios prácticos con acceso a material ya desarrollado y probado
GCIRS 7, a pulsating M1 supergiant at the Galactic centre. Physical properties and age
The stellar population in the central parsec of the Galaxy is dominated by an
old (several Gyr) population, but young, massive stars dominate the luminosity
function. We have studied the most luminous of these stars, GCIRS 7, in order
to constrain the age of the recent star formation event in the Galactic Centre
and to characterise it as an interferometric reference for observations of the
Galactic Centre with the instrument GRAVITY, which will equip the Very Large
Telescope Interferometer in the near future. We present the first H-band
interferometric observations of GCIRS 7, obtained using the PIONIER visitor
instrument on the VLTI using the four 8.2-m unit telescopes. In addition, we
present unpublished K-band VLTI/AMBER data, build JHKL light-curves based on
data spanning 4 decades, and measured the star's effective temperature using
SINFONI spectroscopy. GCIRS 7 is marginally resolved at H-band (in 2013:
uniform-disk diameter=1.076+/-0.093mas, R=960+/-92Rsun at 8.33+/-0.35kpc). We
detect a significant circumstellar contribution at K-band. The star and its
environment are variable in brightness and in size. The photospheric H-band
variations are well modelled with two periods: P0~470+/-10 days (amplitude
~0.64mag) and long secondary period LSP~2700-2850 days (~1.1mag). As measured
from CO equivalent width, =3600+/-195K. The size, periods, luminosity
(=-8.44+/-0.22) and effective temperature are consistent with an M1
supergiant with an initial mass of 22.5+/-2.5Msun and an age of 6.5-10Myr
(depending on rotation). This age is in remarkable agreement with most
estimates for the recent star formation event in the central parsec. Caution
should be taken when using this star as an interferometric reference as it is
variable in size, is surrounded by a variable circumstellar environment and
large convection cells may form on its photosphere.Comment: Accepted for publication in A&A. 10 pages, 12 figure
Sphingolipids and impaired hypoxic stress responses in Huntington disease.
Huntington disease (HD) is a debilitating, currently incurable disease. Protein aggregation and metabolic deficits are pathological hallmarks but their link to neurodegeneration and symptoms remains debated. Here, we summarize alterations in the levels of different sphingolipids in an attempt to characterize sphingolipid patterns specific to HD, an additional molecular hallmark of the disease. Based on the crucial role of sphingolipids in maintaining cellular homeostasis, the dynamic regulation of sphingolipids upon insults and their involvement in cellular stress responses, we hypothesize that maladaptations or blunted adaptations, especially following cellular stress due to reduced oxygen supply (hypoxia) contribute to the development of pathology in HD. We review how sphingolipids shape cellular energy metabolism and control proteostasis and suggest how these functions may fail in HD and in combination with additional insults. Finally, we evaluate the potential of improving cellular resilience in HD by conditioning approaches (improving the efficiency of cellular stress responses) and the role of sphingolipids therein. Sphingolipid metabolism is crucial for cellular homeostasis and for adaptations following cellular stress, including hypoxia. Inadequate cellular management of hypoxic stress likely contributes to HD progression, and sphingolipids are potential mediators. Targeting sphingolipids and the hypoxic stress response are novel treatment strategies for HD
The carbon footprint of large astronomy meetings
The annual meeting of the European Astronomical Society took place in Lyon,
France, in 2019, but in 2020 it was held online only due the COVID-19 pandemic.
The carbon footprint of the virtual meeting was roughly 3,000 times smaller
than the face-to-face one, providing encouragement for more ecologically minded
conferencing.Comment: Originated in a Twitter discussion
(https://twitter.com/sarahkendrew/status/1144186571538739202) at EWASS 2019;
followed up at the EAS 2020 conference sustainability session by
https://astronomersforplanet.earth - published in Nature Astronomy, September
202
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