IR and RI: 外部システムからのスムーズな利用

DRF International Conference 2008 Open Access and Institutional Repository in Asia-Pacific (DRFIC 2008), 30th and 31st January, Osaka, JAPAN / デジタルリポジトリ連合国際会議2008 アジア・環太平洋地域におけるオープンアクセスと機関リポジトリ(DRFIC 2008), 平成20年1月30‐31日, 大阪大学 Poster Session No.5-poster / ポスターセッション No.5-ポスタ

EFEITO DO ESCORE DE CONDIÇÃO CORPORAL(ECC) SOBRE PARÂMETROS REPRODUTIVOS DE VACAS (Bos indicus) SUBMETIDAS À INSEMINAÇÂO ARTIFICIAL EM TEMPO FIXO (IATF)

This study evaluated the effect of BCS on reproductive parameters of Bos indicus cows submitted to IATF. Assessment data from farms in the states of Mato Grosso, São Paulo and Minas Gerais was performed. The animals were analyzed for BCS (scale 1-5) and correlated with the data of follicular dynamics and fertility obtained by synchronization protocol for TAI. Sonographic examinations were performed every 12 hours from implant removal to ovulation for evaluation of follicular dynamics. To fertility, sonographic examinations were performed on the withdrawal of the intravaginal device (D8) at the time of TAI (D10) and diagnosis of pregnancy (D40). Further, the rate of estrus was estimated to be considered when the animals showed a marked on the base of the tail at the moment of AI. It was observed that the diameter of the follicle during the removal of the device and the maximum diameter of the dominant follicle was lower in BCS 2.5 (7.4 ± 0.4, 9.7 ± 0.8, respectively) than in cows BCS 3.0 (9.4 ± 0.3, 12.7 ± 0.5, respectively). In addition, the ovulation rate was lower in the group of cows with low score (BCS = 2.5). To evaluate fertility, animals with BCS 2.5 had lower occurrence of estrus (47.6 %) and lower pregnancy rate (27.1 %) than cows with score 3 (62.1 % and 39.4 %) and 3.5 (67.5% and 45.3 %). We conclude that low ECC can affect reproductive parameters of Bos indicus compromising the reproductive efficiency of these animals.Objetivou-se com este estudo avaliar o efeito do ECC sobre parâmetros reprodutivos de vacas Bos indicus submetidas à IATF. Foi realizada a avaliação de dados de fazendas localizadas nos estados de Mato Grosso, São Paulo e Minas Gerais. Os animais foram analisados quanto ao ECC (escala 1-5) e correlacionados com os dados de dinâmica folicular e fertilidade obtidos pelo do protocolo de sincronização da IATF. Exames ultrassonográficos foram realizados a cada 12 horas a partir da retirada do implante até a ovulação para avaliação da dinâmica folicular. Para avaliação da fertilidade, exames ultrassonográficos foram realizados no dia da retirada do dispositivo intravaginal (D8), no momento da IATF (D10) e diagnóstico de gestação (D40). A taxa de estro foi estimada sendo a mesma considerada quando os animais não apresentavam a base da cauda marcada no momento da IA. Observou-se que o diâmetro do folículo na retirada do dispositivo e o diâmetro máximo do folículo dominante foram menores nas vacas com ECC 2,5 (7,4±0,4; 9,7±0,8, respectivamente) do que nas vacas de ECC 3,0 (9,4±0,3; 12,7±0,5, respectivamente). Além disso, a taxa de ovulação foi menor no grupo de vacas com baixo escore (ECC=2,5). Na avaliação da fertilidade, animais de ECC 2,5 apresentaram menor taxa de cio (47,6%) e menor taxa de prenhez (27,1%) do que vacas com de escore 3 (62,1% e 39,4%) e 3,5 (67,5% e 45,3%). Conclui-se que o baixo ECC é capaz de afetar parâmetros reprodutivos de vacas Bos indicus comprometendo a eficiência reprodutiva desses animais

Pharmacological Potential of Cilostazol for Alzheimer’s Disease

Alzheimer’s disease (AD), a slow progressive form of dementia, is clinically characterized by cognitive dysfunction and memory impairment and neuropathologically characterized by the accumulation of extracellular plaques containing amyloid β-protein (Aβ) and neurofibrillary tangles containing tau in the brain, with neuronal degeneration and high level of oxidative stress. The current treatments for AD, e.g., acetylcholinesterase inhibitors (AChEIs), have efficacies limited to symptom improvement. Although there are various approaches to the disease modifying therapies of AD, none of them can be used alone for actual treatment, and combination therapy may be needed for amelioration of the progression. There are reports that cilostazol (CSZ) suppressed cognitive decline progression in patients with mild cognitive impairment or stable AD receiving AChEIs. Previously, we showed that CSZ suppressed Aβ-induced neurotoxicity in SH-SY5Y cells via coincident inhibition of oxidative stress, as demonstrated by reduced activity of nicotinamide adenine dinucleotide phosphate oxidase, accumulation of reactive oxygen species, and signaling of mitogen-activated protein kinase. CSZ also rescued cognitive impairment and promoted soluble Aβ clearance in a mouse model of cerebral amyloid angiopathy. Mature Aβ fibrils have long been considered the primary neurodegenerative factors in AD; however, recent evidence indicates soluble oligomers to initiate the neuronal and synaptic dysfunction related to AD and other protein-misfolding diseases. Further underscoring the potential of CSZ for AD treatment, we recently described the inhibitory effects of CSZ on Aβ oligomerization and aggregation in vitro. In this review, we discuss the possibility of CSZ as a potential disease-modifying therapy for the prevention or delay of AD

Anti-Aggregation Effects of Phenolic Compounds on α-Synuclein

The aggregation and deposition of α-synuclein (αS) are major pathologic features of Parkinson\u27s disease, dementia with Lewy bodies, and other α-synucleinopathies. The propagation of αS pathology in the brain plays a key role in the onset and progression of clinical phenotypes. Thus, there is increasing interest in developing strategies that attenuate αS aggregation and propagation. Based on cumulative evidence that αS oligomers are neurotoxic and critical species in the pathogenesis of α-synucleinopathies, we and other groups reported that phenolic compounds inhibit αS aggregation including oligomerization, thereby ameliorating αS oligomer-induced cellular and synaptic toxicities. Heterogeneity in gut microbiota may influence the efficacy of dietary polyphenol metabolism. Our recent studies on the brain-penetrating polyphenolic acids 3-hydroxybenzoic acid (3-HBA), 3,4-dihydroxybenzoic acid (3,4-diHBA), and 3-hydroxyphenylacetic acid (3-HPPA), which are derived from gut microbiota-based metabolism of dietary polyphenols, demonstrated an in vitro ability to inhibit αS oligomerization and mediate aggregated αS-induced neurotoxicity. Additionally, 3-HPPA, 3,4-diHBA, 3-HBA, and 4-hydroxybenzoic acid significantly attenuated intracellular αS seeding aggregation in a cell-based system. This review focuses on recent research developments regarding neuroprotective properties, especially anti-αS aggregation effects, of phenolic compounds and their metabolites by the gut microbiome, including our findings in the pathogenesis of α-synucleinopathies

IR and RI: 外部システムからのスムーズな利用

DRF International Conference 2008 Open Access and Institutional Repository in Asia-Pacific (DRFIC 2008), 30th and 31st January, Osaka, JAPAN / デジタルリポジトリ連合国際会議2008 アジア・環太平洋地域におけるオープンアクセスと機関リポジトリ(DRFIC 2008), 平成20年1月30‐31日, 大阪大学　Poster Session No.5-presentation slide / ポスターセッション No.5-口頭発表スライ

IR and RI: 外部システムからのスムーズな利用

In this paper, cooperation of an institutional repository system (hereafter, IR) and the Kyushu University Researcher Information System (hereafter, RI), which is an external system which publishes researcher\u27s information within the institution, is introduced based on literature [1, 2], and the present operation result is described. RI has the common data with IR for literature information, and the data is rich where the rate of input is 99%. The increase of a user of RI can be expected by cooperating so that a user can both use IR and IR unconsciously. However, there are problems: (1) a literature information on both does not exactly match, (2) we should minimize the increase of the time and the labor of users or an administrator, (3) the change to the both existing systems should be minimum, and (4)researcher\u27s intention should be reflected as much as possible. For the problems, we took an approach of not relying only on a static link, but the dynamic one by a searching scheme, resulting in minimizing the database for linking, which only manages the links intended by researchers. / 本論文では，機関リポジトリシステムと，機関研究者の情報を公開する外部システムである九州大学研究者情報システム（以下，研究者情報）との連携事例を文献[1,2]に基づき紹介し，現在の運用状況を述べる．研究者情報は，文献情報という機関リポジトリと共通のデータを持ち，データ入力率が99％と充実している．利用者が知らず知らず機関リポジトリと研究者情報の両方利用できるように連携することで，機関リポジトリの利用者増を見込むことができる．ただし，この連携には，（1）双方の文献情報を一意に紐づけることができない，（2）利用者や管理者の手間を極力増やさない，（3）既存の両システムにできるだけ変更を加えない，（4）研究者の意思をできるだけ反映する，といった課題がある．我々は，システム間の連携を静的なリンクではなく，検索による動的なリンクにより紐付けのためのデータベースを極力小さく押さえながら，研究者の意図する紐付けだけはデータベースで管理する，という方法をとった．DRF International Conference 2008 Open Access and Institutional Repository in Asia-Pacific (DRFIC 2008), 30th and 31st January, Osaka, JAPAN / デジタルリポジトリ連合国際会議2008 アジア・環太平洋地域におけるオープンアクセスと機関リポジトリ(DRFIC 2008), 平成20年1月30‐31日, 大阪大学 Poster Session No.5-Abstract / ポスターセッション No.5-予稿集抄

Comparative Study on Epstein-Barr Virus-Positive Mucocutaneous Ulcer and Methotrexate-Associated Lymphoproliferative Disorders Developed in the Oral Mucosa: A Case Series of 10 Patients and Literature Review

Methotrexate-associated lymphoproliferative disorder (MTX-LPD) is an iatrogenic immunodeficiency-associated lymphoproliferative disorder that occurs mainly with MTX use. This disorder has been associated with Epstein-Barr virus (EBV) infection. In 2017, the WHO newly defined the disease concept of EBV-positive mucocutaneous ulcer (EBV-MCU) as a good-prognosis EBV-related disease. Here, we report 10 cases of MTX-LPD or EBV-MCU in the oral mucosa. This retrospective, observational study was conducted with MTX-LPD or EBV-MCU in the oral mucosa patients who visited us during the nine year period from 2012 to 2021. We gathered the basic information, underlying disease, histopathological evaluation, treatment and prognosis for the subjects. All were being treated with MTX for rheumatoid arthritis. EBV infection was positive in all cases by immunohistochemistry. A complete or partial response was obtained in all cases with the withdrawal of MTX. Our results suggests that the most common risk factor for developing EBV-MCU is the use of immunosuppressive drugs. The most common site of onset is the oral mucosa, which may be attributed to the mode of EBV infection and the high incidence of chronic irritation of the oral mucosa. A small number of patients had been diagnosed with MTX-LPD, but we consider that these cases were EBV-MCU based on our study

Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum

<p>Abstract</p> <p>Background</p> <p>Cerebellar corticogenesis begins with the assembly of Purkinje cells into the Purkinje plate (PP) by embryonic day 14.5 (E14.5) in mice. Although the dependence of PP formation on the secreted protein Reelin is well known and a prevailing model suggests that Purkinje cells migrate along the 'radial glial' fibers connecting the ventricular and pial surfaces, it is not clear how Purkinje cells behave in response to Reelin to initiate the PP. Furthermore, it is not known what nascent Purkinje cells look like <it>in vivo</it>. When and how Purkinje cells start axonogenesis must also be elucidated.</p> <p>Results</p> <p>We show that Purkinje cells generated on E10.5 in the posterior periventricular region of the lateral cerebellum migrate tangentially, after only transiently migrating radially, towards the anterior, exhibiting an elongated morphology consistent with axonogenesis at E12.5. After their somata reach the outer/dorsal region by E13.5, they change 'posture' by E14.5 through remodeling of non-axon (dendrite-like) processes and a switchback-like mode of somal movement towards a superficial Reelin-rich zone, while their axon-like fibers remain relatively deep, which demarcates the somata-packed portion as a plate. In <it>reeler </it>cerebella, the early born posterior lateral Purkinje cells are initially normal during migration with anteriorly extended axon-like fibers until E13.5, but then fail to form the PP due to lack of the posture-change step.</p> <p>Conclusions</p> <p>Previously unknown behaviors are revealed for a subset of Purkinje cells born early in the posteior lateral cerebellum: tangential migration; early axonogenesis; and Reelin-dependent reorientation initiating PP formation. This study provides a solid basis for further elucidation of Reelin's function and the mechanisms underlying the cerebellar corticogenesis, and will contribute to the understanding of how polarization of individual cells drives overall brain morphogenesis.</p

Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan

<p>Abstract</p> <p>Background</p> <p>We performed an environmental study of viruses infecting the symbiotic single-celled algae of <it>Paramecium bursaria </it>(<it>Paramecium bursaria Chlorella </it>virus, PBCV) in Lake Biwa, the largest lake in Japan. The viruses detected were all <it>Chlorella variabilis </it>virus (CvV = NC64A virus). One of them, designated CvV-BW1, was subjected to further characterization.</p> <p>Results</p> <p>CvV-BW1 formed small plaques and had a linear DNA genome of 370 kb, as judged by pulsed-field gel electrophoresis. Restriction analysis indicated that CvV-BW1 DNA belongs to group H, one of the most resistant groups among CvV DNAs. Based on a phylogenetic tree constructed using the <it>dnapol </it>gene, CvV was classified into two clades, A and B. CvV-BW1 belonged to clade B, in contrast to all previously identified virus strains of group H that belonged to clade A.</p> <p>Conclusions</p> <p>We conclude that CvV-BW1 composes a distinct species within <it>C. variabilis </it>virus.</p

Murine liver allograft transplantation: Tolerance and donor cell chimerism

Nonarterialized orthotopic liver transplantation with no immunosuppression was performed in 13 mouse‐strain combinations. Two strain combinations with major histocompatibility complex class I and class II and minor histocompatibility complex disparity had 20% and 33% survival of more than 100 days, but the other 11 combinations, including four that were fully allogeneic and all with only class I, class II or minor disparities, yielded 45% to 100% survival of more than 100 days. Long‐living recipients permanently accepted donor‐strain heterotopic hearts transplanted on the same day or donor‐strain skin 3 mo after liver transplantation, in spite of detectable antidonor in vitro activity with mixed lymphocyte reaction and cellmediated lymphocytotoxicity testing (split tolerance). In further donor‐specific experiments, liver grafts were not rejected by presensitized major histocompatibility complex class I‐disparate recipients and they protected donor‐strain skin grafts from second set (or any) rejection. Less frequently, liver transplantation rescued rejecting skin grafts placed 1 wk earlier in major histocompatibility complex class I, class II and minor histocompatibility complex, class II or minor histocompatibility complex‐disparate strain combinations. Donor‐derived leukocyte migration to the central lymphoid organs occurred within 1 to 2 hr after liver transplantation in all animals examined, persisted in the surviving animals until they were killed (>375 days), and was demonstrated with double‐immunolabeling to be multilineage. The relation of these findings to so‐called hepatic tolerogenicity and to tolerance in general is discussed. (HEPATOLOGY 1994;19:916–924.) Copyright © 1994 American Association for the Study of Liver Disease