Feasibility Study of a 3D CFD Solution for FSI Investigations on NREL 5MW Wind Turbine Blade

With the increase in length of wind turbine blades flutter is becoming a potential design constrain, hence the interest in computational tools for fluid-structure interaction studies. The general approach to this problem makes use of simplified aerodynamic computational tools. Scope of this work is to investigate the outcomes of a 3D CFD simulation of a complete wind turbine blade, both in terms of numerical results and computational cost. The model studied is a 5MW theoretical wind turbine from NREL. The simulation was performed with ANSYS-CFX, with different volume mesh and turbulence model, in steady-state and transient mode. The convergence history and computational time was analyzed, and the pressure distribution was compared to a high fidelity numerical result of the same blade. All the model studied were about two orders of magnitude lighter than the reference in computation time, while showing comparable results in most of the cases. The results were affected more by turbulence model than mesh density, and some turbulence models did not converge to a solution. In general seems possible to obtain good results from a complete 3D CFD simulation while keeping the computational cost reasonably low. Attention should be paid to mesh quality

Relay of Herpes Simplex Virus between Langerhans Cells and Dermal Dendritic Cells in Human Skin

<div><p>The mechanism by which immunity to Herpes Simplex Virus (HSV) is initiated is not completely defined. HSV initially infects mucosal epidermis prior to entering nerve endings. In mice, epidermal Langerhans cells (LCs) are the first dendritic cells (DCs) to encounter HSV, but it is CD103⁺ dermal DCs that carry viral antigen to lymph nodes for antigen presentation, suggesting DC cross-talk in skin. In this study, we compared topically HSV-1 infected human foreskin explants with biopsies of initial human genital herpes lesions to show LCs are initially infected then emigrate into the dermis. Here, LCs bearing markers of maturation and apoptosis formed large cell clusters with BDCA3⁺ dermal DCs (thought to be equivalent to murine CD103⁺ dermal DCs) and DC-SIGN⁺ DCs/macrophages. HSV-expressing LC fragments were observed inside the dermal DCs/macrophages and the BDCA3⁺ dermal DCs had up-regulated a damaged cell uptake receptor CLEC9A. No other infected epidermal cells interacted with dermal DCs. Correspondingly, LCs isolated from human skin and infected with HSV-1 <i>in vitro</i> also underwent apoptosis and were taken up by similarly isolated BDCA3⁺ dermal DCs and DC-SIGN⁺ cells. Thus, we conclude a viral antigen relay takes place where HSV infected LCs undergo apoptosis and are taken up by dermal DCs for subsequent antigen presentation. This provides a rationale for targeting these cells with mucosal or perhaps intradermal HSV immunization.</p></div

Migration and/or interaction of HSV infected human LCs with dermal DCs.

<p>(A) Migration of LCs and dermal DCs out of skin after topical HSV application to inner foreskin explants. (B) Summary diagram of interaction between HSV infected LCs and dermal DCs in human skin.</p