691 research outputs found
Non-canonical auxin signalling : fast and curious
Many plant biologists might think that the auxin signalling pathway has been resolved. Activation of gene expression as a result of indole-3-acetic acid (IAA)-mediated assembly of Transport Inhibitor1 (TIR1)/Auxin F-Box (AFB) proteins with AUX/IAA transcriptional regulators has become accepted as the canonical auxin signalling pathway. However, the evidence strongly suggests that non-canonical pathways will still prove to be important, and this theme ran through the 2018 Auxins and Cytokinins in Plant Development conference held in Prague (ACPD 2018)
Stress management and organisation development : effects of transcendental meditation on psychological, physiological, and organisational variables at the worksite
Bibliography: p. 348-363.This study evaluated the effectiveness of a stress reduction intervention (SRI) offered to employees at one worksite where 80 were employed. 41 Volunteers (aged 21-65) participated in Transcendental Meditation (TM), and 18 (aged 19-46) in Progressive Muscle Relaxation (PMR). Two groups did not attend the SRI. These were 11 non-volunteers for the SRI (aged 25-58) who served as on-site controls, while 16 outside attendees (aged 27-44) of a personal productivity workshop served as offsite controls. All subjects completed a standardised stress symptoms questionnaire (SCL-90-R) before and after the SRI. On-site subjects also had blood pressure, heart and breath rates measured by a trained nurse and completed a company climate questionnaire-before and after the SRI. Structured interviews were conducted at three year followup. An hypothesis that test groups would show significant reductions in psychological stress symptoms was supported at 6-week (TM p<0,0001, PMR p<0,001) and 5,5 month followup (p<0,0002, paired-t). TM groups showed significant reductions in blood pressure at 5,5 month followup (p<0,05). Overall company climate showed improvement at 6 weeks which tended to reverse at 5,5 months, following retrenchment of 10 staff. Psychological and physiological variables were found to be positively correlated. Non-volunteer on-site controls appeared to benefit almost as much as those who received training, whereas off-site controls did not. Company effectiveness improved over three years following the intervention though causality was not addressed
Factors Associated with Blockages to Outdoor Recreation Participation: A State Survey
Data from a random sample of Ohio residents were used in an attempt to isolate factors that were hypothesized to be related to outdoor recreation blockages. The study revealed that socio-demographic variables failed to explain the variance in factor scores created to measure blockages to outdoor recreation. Findings from descriptive statistics and the factor analyses are discussed in a planning context
Nucleic acid aptamers as aptasensors for plant biology
Our knowledge of cell- and tissue-specific quantification of phytohormones is heavily reliant on laborious mass spectrometry techniques. Genetically encoded biosensors have allowed spatial and some temporal quantification of phytohormones intracellularly, but there is still limited information on their intercellular distributions. Here, we review nucleic acid aptamers as an emerging biosensing platform for the detection and quantification of analytes with high affinity and specificity. Options for DNA aptamer technology are explained through selection, sequencing analysis and techniques for evaluating affinity and specificity, and we focus on previously developed DNA aptamers against various plant analytes. We suggest how these tools might be applied in planta for quantification of molecules of interest both intracellularly and intercellularly
Fractional boundary value problems: Analysis and numerical methods
This is the author's PDF of an article published in Fractional calculus and applied analysis 2011. The original publication is available at www.springerlink.comThis journal article discusses nonlinear boundary value problems.Fundacao para a Ciencia e Tecnologi
Tomographic docking suggests the mechanism of auxin receptor TIR1 selectivity
We study the binding of plant hormone IAA on its receptor TIR1 introducing a
novel computational method that we call tomographic docking and that accounts
for interactions occurring along the depth of the binding pocket. Our results
suggest that selectivity is related to constraints that potential ligands
encounter on their way from the surface of the protein to their final position
at the pocket bottom. Tomographic docking helps develop specific hypotheses
about ligand binding, distinguishing binders from non-binders, and suggests
that binding is a three-step mechanism, consisting of engagement with a niche
in the back wall of the pocket, interaction with a molecular filter which
allows or precludes further descent of ligands, and binding on the pocket base.
Only molecules that are able to descend the pocket and bind at its base allow
the co-receptor IAA7 to bind on the complex, thus behaving as active auxins.
Analyzing the interactions at different depths, our new method helps in
identifying critical residues that constitute preferred future study targets
and in the quest for safe and effective herbicides. Also, it has the potential
to extend the utility of docking from ligand searches to the study of processes
contributing to selectivity.Comment: 11 pages, 7 figure
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