Evaluation of CD4 count and correlation with development of opportunistic infection among HIV seropositives

Background: Opportunistic infections have been the important indicators to suspect and diagnose the HIV seropositives. CD4 count has been used an important tool to monitor the treatment and progression of disease. Methods: This study was done to study the pattern or common presentations of opportunistic infections among HIV seropositive patients and their relationship with CD4 counts. Results: Tuberculosis and candidiasis were the most common opportunistic infections in HIV infected patients followed by herpes, diarrhoea, acute respiratory infection, papular pruritic eruptions, seborrhoeic dermatitis, cryptococcal meningitis, and hepatitis B and C co-infection. Decrease in CD4 count is an important predictor to progression of the disease. Conclusions: This gives a representation of opportunistic infections in HIV seropositive patients in low prevalence state like Jharkhand

Modelling of sPn phases for reliable estimation of focal depths in northeastern India

In North East India, reliable estimation of earthquake focal depths has always been a problem, owing to paucity of permanent regional seismic stations, constraints on good quality data and lack of crustal models in a complex tectonic terrane. The depth estimations by international agencies, mostly based on teleseismic data, vary from very shallow to even 60 km, suggestive of earthquake occurrence in the upper mantle region. However, modelling of sPn phases in earthquake waveforms that are highly sensitive to focal depths indicates that the earthquake locations are probably well within the crustal layer. The current method has the advantage that dt, the travel time difference between sPn and Pn, remains constant for a wide range of source-station distances, and hence enables easy identification of the sPn phase, while providing direct and accurate estimate of the focal depth. The approach is also insensitive to location errors and variations in crustal models, a useful feature especially while dealing with sparse data with high location errors. In the present study, earthquakes in the magnitude range 3.0-4.0 recorded by a network of nine broadband stations in NE India have been analysed. Consistent dt values at different stations were observed for each event, enabling precise depth estimation mostly within ±1 or 2 km. In general, depths of 15-20 km in the Shillong plateau region, >20 km in the Sylhet basin region and shallow <10 km in the eastern Himalayan foothills are confirmed, that correlate well with the local tectonics. Further, we propose that the flat characteristics of the dt curve that begin to change for earthquakes below the Moho, can be a potential tool for discriminating between crustal and sub-crustal earthquakes, as well as for delineation of the Moho using dense regional seismic networks in future

SEISMIC BRIDGE PIER ANALYSIS FOR PILE FOUNDATION BY FORCE AND DISPLACEMENT BASED APPROACHES

Seismic analysis of bridge pier supported on pile foundation requires consideration of soil-pile-structure (kinematic and inertial) interactions. This paper presents the design forces generated for bridge piers with varying height and constant diameter for medium and soft soils in earthquake probability zones considering contribution of soil-pile-structure interactions by developed analytical approaches. The results have shown that the difference in base shear demand between force based and displacement based approach and that between capacity spectrum and displacement based method in general decreases with the increase in slenderness ratio of the pier. The base shear demand by non-linear time history analysis has been found to be much higher compared to that by other methods. The relationship between height and pier cross-section has been developed for different soils and seismic zones such that the base shear demands by force based and displacement based method are of the same order. The overall value of the slenderness ratio works out to be such that failure of the pile shall be as a short column for both medium and soft soil

Gastric and pulmonary lymphoma presenting as a solitary pulmonary nodule

The common presentations of lymphoma are widespread lymphadenopathy or development of constitutional symptoms. This paper presents a case of a patient who presented with a solitary mass detected on chest X-ray and underwent FDG-PET for further evaluation of this mass. FDG-PET is a commonly utilised technique to assess solitary nodules as it not only allows characterisation of the lesion but can also detect nodal and extra-thoracic disease with greater accuracy than the standard CT. In this case, FDG-PET demonstrated abnormal activity in the lung nodule and at the gastro-oesophageal junction. Biopsies confirmed Non-Hodgkin’s Lymphoma at both sites. The value of FDG-PET in this case was the determination of previous unsuspected disease in an unusual presentation of lymphoma and as a useful tool for monitoring the therapeutic effect post chemotherapy

Therapeutic effect of hydroethanolic extract of Trianthema portulacastrum L. against N-Nitroso-N-Methylurea-induced mammary tumors in Wistar rats

406-415This study evaluated the therapeutic action of hydroethanolic extract of Trianthema portulacastrum L. (TPE) on N-nitroso-N-methylurea (NMU)-induced mammary tumors in Wistar rats. A hydroethanolic was prepared and subjected to qualitative and quantitative phytochemical screening. After acclimatization, Wistar rats were divided into 4 groups of 6 rats each: Group A (vehicle control), Group B (TPE control), Group C (TPE treatment) and group D (NMU control). NMU (50 mg/kg body weight) was injected intraperitoneally at 50, 80 and 110 days of age. After the induction of palpable tumors, the rats were administered 200 mg/kg bw of TPE by oral gavage for 2 months. The treatment with TPE significantly (pin vivo therapeutic action of TPE extract on NMU-induced mammary tumors. TPE exhibited antitumor activity through its antiproliferative, antiangiogenic, pro-apoptotic, and estrogen receptor-modulatory properties

PKC Theta Ablation Improves Healing in a Mouse Model of Muscular Dystrophy

Inflammation is a key pathological characteristic of dystrophic muscle lesion formation, limiting muscle regeneration and resulting in fibrotic and fatty tissue replacement of muscle, which exacerbates the wasting process in dystrophic muscles. Limiting immune response is thus one of the therapeutic options to improve healing, as well as to improve the efficacy of gene- or cell-mediated strategies to restore dystrophin expression. Protein kinase C θ (PKCθ) is a member of the PKCs family highly expressed in both immune cells and skeletal muscle; given its crucial role in adaptive, but also innate, immunity, it is being proposed as a valuable pharmacological target for immune disorders. In our study we asked whether targeting PKCθ could represent a valuable approach to efficiently prevent inflammatory response and disease progression in a mouse model of muscular dystrophy. We generated the bi-genetic mouse model mdx/θ−/−, where PKCθ expression is lacking in mdx mice, the mouse model of Duchenne muscular dystrophy. We found that muscle wasting in mdx/θ−/− mice was greatly prevented, while muscle regeneration, maintenance and performance was significantly improved, as compared to mdx mice. This phenotype was associated to reduction in inflammatory infiltrate, pro-inflammatory gene expression and pro-fibrotic markers activity, as compared to mdx mice. Moreover, BM transplantation experiments demonstrated that the phenotype observed was primarily dependent on lack of PKCθ expression in hematopoietic cells

Skeletal Muscle NADPH Oxidase Is Increased and Triggers Stretch-Induced Damage in the mdx Mouse

Recent studies have shown that oxidative stress contributes to the pathogenesis of muscle damage in dystrophic (mdx) mice. In this study we have investigated the role of NADPH oxidase as a source of the oxidative stress in these mice. The NADPH oxidase subunits gp91phox, p67phox and rac 1 were increased 2–3 fold in tibilais anterior muscles from mdx mice compared to wild type. Importantly, this increase occurred in 19 day old mice, before the onset of muscle necrosis and inflammation, suggesting that NADPH oxidase is an important source of oxidative stress in mdx muscle. In muscles from 9 week old mdx mice, gp91phox and p67phox were increased 3–4 fold and NADPH oxidase superoxide production was 2 times greater than wild type. In single fibers from mdx muscle NADPH oxidase subunits were all located on or near the sarcolemma, except for p67phox,which was expressed in the cytosol. Pharmacological inhibition of NADPH oxidase significantly reduced the intracellular Ca2+ rise following stretched contractions in mdx single fibers, and also attenuated the loss of muscle force. These results suggest that NADPH oxidase is a major source of reactive oxygen species in dystrophic muscle and its enhanced activity has a stimulatory effect on stretch-induced Ca2+ entry, a key mechanism for muscle damage and functional impairment

Capsular profiling of the Cronobacter genus and the association of specific Cronobacter sakazakii and C. malonaticus capsule types with neonatal meningitis and necrotizing enterocolitis

Background: Cronobacter sakazakii and C. malonaticus can cause serious diseases especially in infants where they are associated with rare but fatal neonatal infections such as meningitis and necrotising enterocolitis. Methods: This study used 104 whole genome sequenced strains, covering all seven species in the genus, to analyse capsule associated clusters of genes involved in the biosynthesis of the O-antigen, colanic acid, bacterial cellulose, enterobacterial common antigen (ECA), and a previously uncharacterised K-antigen. Results: Phylogeny of the gnd and galF genes flanking the O-antigen region enabled the defining of 38 subgroups which are potential serotypes. Two variants of the colanic acid synthesis gene cluster (CA1 and CA2) were found which differed with the absence of galE in CA2. Cellulose (bcs genes) were present in all species, but were absent in C. sakazakii sequence type (ST) 13 and clonal complex (CC) 100 strains. The ECA locus was found in all strains. The K-antigen capsular polysaccharide Region 1 (kpsEDCS) and Region 3 (kpsMT) genes were found in all Cronobacter strains. The highly variable Region 2 genes were assigned to 2 homology groups (K1 and K2). C. sakazakii and C. malonaticus isolates with capsular type [K2:CA2:Cell+] were associated with neonatal meningitis and necrotizing enterocolitis. Other capsular types were less associated with clinical infections. Conclusion: This study proposes a new capsular typing scheme which identifies a possible important virulence trait associated with severe neonatal infections. The various capsular polysaccharide structures warrant further investigation as they could be relevant to macrophage survival, desiccation resistance, environmental survival, and biofilm formation in the hospital environment, including neonatal enteral feeding tubes

Genotyping of Human Lice Suggests Multiple Emergences of Body Lice from Local Head Louse Populations

While being phenotypically and physiologically different, human head and body lice are indistinguishable based on mitochondrial and nuclear genes. As protein-coding genes are too conserved to provide significant genetic diversity, we performed strain-typing of a large collection of human head and body lice using variable intergenic spacer sequences. Ninety-seven human lice were classified into ninety-six genotypes based on four intergenic spacer sequences. Genotypic and phylogenetic analyses using these sequences suggested that human head and body lice are still indistinguishable. We hypothesized that the phenotypic and physiological differences between human head and body lice are controlled by very limited mutations. Under conditions of poor hygiene, head lice can propagate very quickly. Some of them will colonize clothing, producing a body louse variant (genetic or phenetic), which can lead to an epidemic. Lice collected in Rwanda and Burundi, where outbreaks of louse-borne diseases have been recently reported, are grouped tightly into a cluster and those collected from homeless people in France were also grouped into a cluster with lice collected in French non-homeless people. Our strain-typing approach based on highly variable intergenic spacers may be helpful to elucidate louse evolution and to survey louse-borne diseases