Efecto de tratamientos enzim?tico, microondas y ultrasonido en la extracci?n de grasa de semilla de mango (Mangifera Indica L.)

73 P?ginasSe evalu? el efecto de los tratamientos de ultrasonido, microondas y enzim?tico como procesamiento previo al m?todo por prensado para aumentar el rendimiento de extracci?n de la grasa de semilla de mango. Se trabajaron dos variedades de mango Kala Alphonso y Mariquita. Las muestras fueron expuestas a ultrasonido durante 1 h, con una amplitud de 100 % y ciclo de 1 s. El tratamiento con microondas se evalu? a una potencia de 100 W durante 15 y 30 min con intervalos de 5 min evitando la evaporaci?n del agua destilada. En el tratamiento con enzimas se incub? la muestra a 45?C durante 4 h. Los rendimientos se calcularon con base al contenido inicial de aceite determinados por el m?todo Soxhlet para cada variedad 12,0 % para Kala Alphonso y 11,35 % Mariquita. Los resultados mostraron que la semilla Kala Alphonso tratada con enzima present? mayor rendimiento de extracci?n con 33,36 % en comparaci?n con Mariquita (25,99 %). Con el tratamiento de ultrasonido, la variedad Kala Alphonso obtuvo el mayor rendimiento de extracci?n (16,02 %). En el tratamiento con microondas no se logr? obtener unas condiciones de procesamiento donde la exposici?n a la energ?a electromagn?tica no afectara la estructura de la semilla y se pudiera extraer grasa de semilla de mango. De igual forma se realizaron pruebas de extracci?n de componentes grasos por el m?todo de extrusi?n, donde no se obtuvo la separaci?n de la grasa de la semilla, por lo anterior puede explicarse en que este tipo de procesamiento es m?s eficaz cuando se trabaja con semillas con mayor contenido de aceite. Las pruebas de calidad determinaron que la grasa extra?da presenta caracter?sticas similares a grasas industriales, lo que permitir?a su posible futura aplicaci?n en diferentes industrias.ABSTRACT It was evaluated the ultrasound, microwaves and enzymatic treatment effects as previous processing to the press method in order to increase mango seed fat extraction performance. Two varieties of mango, Kala Alphonso and Mariquita, were subjected to different treatments. The samples were exposed to ultrasound waves during 1 h, with 100 % amplitude and 1 s cycle. The microwave treatment was evaluated at minimal power (100 W) during 15 and 30 min with 5 min intervals for avoiding distilled water evaporation. In the treatment with enzymes, the sample was incubated at 45?C during 4 h. The yield was analyzed based on the initial oil content measured by Soxhlet method, 12,0 % for Kala Alphonso and 11,35 % for Mariquita. The results showed that Kala Alphonso seed treated with enzyme had a higher yield with 33,36 % compared to Mariquita with 25,99 %. In the ultrasound treatment, the Kala Alphonso variety had the higher extraction performance (16,02 %). In the microwave treatment was not possible to obtain processing conditions that exposure to electromagnetic energy will not affect the seed structure and the extraction of fat mango kernel. It was also made fat extraction tests by the extrusion method, however it was not possible to obtain fat separation, this can be explained that extrusion processing is most effective with higher oil content seeds. The quality results showed that fat mango seed presented characteristics similar to other industrial fats, so it is possible used in different industries considering its high unsaturated fatty acid content. The mechanical press extraction is an efficient process for obtaining mango seed fat but it is required previous treatments, however this is a real possibility in order to add value to an industrial sub-product.INTRODUCCI?N 12 1. PLANTEAMIENTO DEL PROBLEMA 15 2. JUSTIFICACI?N 16 3. OBJETIVOS 17 3.1 GENERAL 17 3.2 ESPECIFICOS 17 4. MARCO TE?RICO 18 4.1 GENERALIDADES DEL MANGO 18 4.1.1 Producci?n y Comercializaci?n del Mango 18 4.1.2 Subproductos del Procesamiento del Mango 20 4.2 COMPOSICI?N DE LA SEMILLA 21 4.2.1 Compuestos Grasos de la Semilla de Mango 21 4.3 M?TODOS DE EXTRACCI?N 25 4.3.1 M?todos Soxhlet o con Disolventes 25 4.3.2 M?todo de Extracci?n por Prensado 25 4.3.3 Extracci?n Asistida por Microondas 25 4.3.4 Extracci?n Asistida con Ultrasonido 26 4.3.5 Actividad Enzim?tica 26 4.4 ESTADO DEL ARTE 26 5. METODOLOG?A 32 5.1 CARACTERIZACION DE LA SEMILLA DE MANGO 32 5 5.1.1 M?todo de Extracci?n por Soxhlet 34 5.2 PRUEBAS DE HIDRATACI?N DE LA SEMILLA DE MANGO 35 5.2.1 Hidrataci?n con Vapor de Agua 36 5.2.2 Hidrataci?n Semillas en Remojo 36 5.2.3 Hidrataci?n con Vapor en Autoclave 36 5.3 TRATAMIENTOS PREVIOS DE EXTRACCI?N 37 5.3.1 Tratamiento por Microondas 37 5.3.2 Tratamiento por Ultrasonido 37 5.3.3 Tratamiento Enzim?tico 38 5.4 M?TODOS DE EXTRACCI?N 39 5.4.1 M?todo de Extracci?n por Prensado 39 5.4.2. M?todo de Extracci?n por Extrusi?n 41 5.5 DETERMINACI?N DE LA CALIDAD 42 5.6 DISE?O EXPERIMENTAL 42 5.6.1 An?lisis de Resultados 43 6. RESULTADOS Y DISCUSI?N 44 6.1 RENDIMIENTO DE EXTRACCION POR SOXHLET 44 6.1.1 An?lisis Proximal 44 6.1.2 Perfil de ?cidos Grasos 45 6.2 PRUEBAS DE HIDRATACI?N DE LA SEMILLA DE MANGO 46 6.3 TRATAMIENTOS PREVIOS DE EXTRACCI?N POR PRENSADO 48 6.3.1 Tratamiento por Microondas 48 6.3.2 Tratamiento con Ultrasonido y Enzima 49 6.4 DETERMINACI?N DE LA CALIDAD 52 6.5 RENDIMIENTO DE EXTRACCI?N POR EXTRUSI?N 56 7. CONCLUSIONES 58 RECOMENDACIONES 60 6 REFERENCIAS 6

Multicenter Comparison of Molecular Tumor Boards in The Netherlands:Definition, Composition, Methods, and Targeted Therapy Recommendations

Background Molecular tumor boards (MTBs) provide rational, genomics-driven, patient-tailored treatment recommendations. Worldwide, MTBs differ in terms of scope, composition, methods, and recommendations. This study aimed to assess differences in methods and agreement in treatment recommendations among MTBs from tertiary cancer referral centers in The Netherlands. Materials and Methods MTBs from all tertiary cancer referral centers in The Netherlands were invited to participate. A survey assessing scope, value, logistics, composition, decision-making method, reporting, and registration of the MTBs was completed through on-site interviews with members from each MTB. Targeted therapy recommendations were compared using 10 anonymized cases. Participating MTBs were asked to provide a treatment recommendation in accordance with their own methods. Agreement was based on which molecular alteration(s) was considered actionable with the next line of targeted therapy. Results Interviews with 24 members of eight MTBs revealed that all participating MTBs focused on rare or complex mutational cancer profiles, operated independently of cancer type-specific multidisciplinary teams, and consisted of at least (thoracic and/or medical) oncologists, pathologists, and clinical scientists in molecular pathology. Differences were the types of cancer discussed and the methods used to achieve a recommendation. Nevertheless, agreement among MTB recommendations, based on identified actionable molecular alteration(s), was high for the 10 evaluated cases (86%). Conclusion MTBs associated with tertiary cancer referral centers in The Netherlands are similar in setup and reach a high agreement in recommendations for rare or complex mutational cancer profiles. We propose a "Dutch MTB model" for an optimal, collaborative, and nationally aligned MTB workflow. Implications for Practice Interpretation of genomic analyses for optimal choice of target therapy for patients with cancer is becoming increasingly complex. A molecular tumor board (MTB) supports oncologists in rationalizing therapy options. However, there is no consensus on the most optimal setup for an MTB, which can affect the quality of recommendations. This study reveals that the eight MTBs associated with tertiary cancer referral centers in The Netherlands are similar in setup and reach a high agreement in recommendations for rare or complex mutational profiles. The Dutch MTB model is based on a collaborative and nationally aligned workflow with interinstitutional collaboration and data sharing

Changing foreign policy: the Obama Administration’s decision to oust Mubarak

This paper analyses the decision of the Obama administration to redirect its foreign policy towards Egypt in the wake of the Arab Spring. It attempts to highlight the issue of how governments deal with decision-making at times of crisis, and under which circumstances they take critical decisions that lead to major shifts in their foreign policy track record. It focuses on the process that led to a reassessment of US (United States) foreign policy, shifting from decades of support to the autocratic regime of Hosni Mubarak, towards backing his ouster. Specifically, the paper attempts to assess to what extent the decision to withdraw US support from a longstanding state-leader and ally in the Middle East can be seen as a foreign policy change (FPC). A relevant research question this paper pursues is: how can the withdrawal of US support to a regime considered as an ally be considered, in itself, as a radical FPC

Life-prolonging treatment restrictions and outcomes in patients with cancer and COVID-19:an update from the Dutch Oncology COVID-19 Consortium

AIM OF THE STUDY: The coronavirus disease 2019 (COVID-19) pandemic significantly impacted cancer care. In this study, clinical patient characteristics related to COVID-19 outcomes and advanced care planning, in terms of non-oncological treatment restrictions (e.g. do-not-resuscitate codes), were studied in patients with cancer and COVID-19. METHODS: The Dutch Oncology COVID-19 Consortium registry was launched in March 2020 in 45 hospitals in the Netherlands, primarily to identify risk factors of a severe COVID-19 outcome in patients with cancer. Here, an updated analysis of the registry was performed, and treatment restrictions (e.g. do-not-intubate codes) were studied in relation to COVID-19 outcomes in patients with cancer. Oncological treatment restrictions were not taken into account. RESULTS: Between 27th March 2020 and 4th February 2021, 1360 patients with cancer and COVID-19 were registered. Follow-up data of 830 patients could be validated for this analysis. Overall, 230 of 830 (27.7%) patients died of COVID-19, and 60% of the remaining 600 patients with resolved COVID-19 were admitted to the hospital. Patients with haematological malignancies or lung cancer had a higher risk of a fatal outcome than other solid tumours. No correlation between anticancer therapies and the risk of a fatal COVID-19 outcome was found. In terms of end-of-life communication, 50% of all patients had restrictions regarding life-prolonging treatment (e.g. do-not-intubate codes). Most identified patients with treatment restrictions had risk factors associated with fatal COVID-19 outcome. CONCLUSION: There was no evidence of a negative impact of anticancer therapies on COVID-19 outcomes. Timely end-of-life communication as part of advanced care planning could save patients from prolonged suffering and decrease burden in intensive care units. Early discussion of treatment restrictions should therefore be part of routine oncological care, especially during the COVID-19 pandemic

Influence of germline variations in drug transporters ABCB1 and ABCG2 on intracerebral osimertinib efficacy in patients with non-small cell lung cancer

BACKGROUND: Central nervous system (CNS) metastases are present in approximately 40% of patients with metastatic epidermal growth factor receptor-mutated ( EGFRm+) non-small cell lung cancer (NSCLC). The EGFR-tyrosine kinase inhibitor osimertinib is a substrate of transporters ABCB1 and ABCG2 and metabolized by CYP3A4. We investigated relationships between single nucleotide polymorphisms (SNPs) ABCB1 3435C>T, ABCG2 421C>A and 34G>A, and CYP3A4∗22 and CNS treatment efficacy of osimertinib in EGFRm+ NSCLC patients. METHODS: Patients who started treatment with osimertinib for EGFRm+ NSCLC between November 2014 and June 2021 were included in this retrospective observational multicentre cohort study. For patients with baseline CNS metastases, the primary endpoint was CNS progression-free survival (CNS-PFS; time from osimertinib start until CNS disease progression or death). For patients with no or unknown baseline CNS metastases, the primary endpoint was CNS disease-free survival (CNS-DFS; time from osimertinib start until occurrence of new CNS metastases). Relationships between SNPs and baseline characteristics with CNS-PFS and CNS-DFS were studied with competing-risks survival analysis. Secondary endpoints were relationships between SNPs and PFS, overall survival, severe toxicity, and osimertinib pharmacokinetics. FINDINGS: From 572 included patients, 201 had baseline CNS metastases. No SNP was associated with CNS-PFS. Genotype ABCG2 34GA/AA and/or ABCB1 3435CC --present in 35% of patients-- was significantly associated with decreased CNS-DFS (hazard ratio 0.28; 95% CI 0.11-0.73; p = 0.009) in the multivariate analysis. This remained significant after applying a Bonferroni correction and internal validation through bootstrapping. ABCG2 421CA/AA was related to more severe toxicity (27.0% versus 16.5%; p = 0.010). INTERPRETATION: ABCG2 34G>A and ABCB1 3435C>T are predictors for developing new CNS metastases during osimertinib treatment, probably because of diminished drug levels in the CNS. ABCG2 421C>A was significantly related with the incidence of severe toxicity. Pre-emptive genotyping for these SNPs could individualize osimertinib therapy. Addition of ABCG2 inhibitors for patients without ABCG2 34G>A should be studied further, to prevent new CNS metastases during osimertinib treatment. FUNDING: No funding was received for this trial

Recommendations for Enhancing Psychosocial Support of NICU Parents through Staff Education and Support

Providing psychosocial support to parents whose infants are hospitalized in the neonatal intensive care unit (NICU) can improve parents’ functioning as well as their relationships with their babies. Yet, few NICUs offer staff education that teaches optimal methods of communication with parents in distress. Limited staff education in how to best provide psychosocial support to families is one factor that may render those who work in the NICU at risk for burnout, compassion fatigue and secondary traumatic stress syndrome. Staff who develop burnout may have further reduced ability to provide effective support to parents and babies. Recommendations for providing NICU staff with education and support are discussed. The goal is to deliver care that exemplifies the belief that providing psychosocial care and support to the family is equal in importance to providing medical care and developmental support to the baby

Analisi dei tempi d\u2019attesa tra le varie fasi di gestione dei carcinomi mammari screening-detected a Trieste nel biennio 2013-2014: come si pu\uf2 migliorare?

Gli indicatori relativi ai tempi di attesa sono difficili da rispettare, come recentemente evidenziato al XIII Convegno ONS 2015 . Per questo motivo \ue8 fondamentale identificare in quale momento della gestione dei carcinomi screening-detected si concentrino i ritardi e stabilirne le cause (se attribuibili alla paziente o all\u2019organizzazione del programma o intrinseci al tipo di lesione) cos\uec da proporre mirate modifiche migliorative. Metodi: L\u2019analisi riguarda 146 carcinomi screening-detected consecutivi (biennio 2013-2014). Sono stati misurati i tempi tra le varie fasi diagnostiche (Mammografia di I\ub0 livello, Richiamo II\ub0 livello, I\ub0 approfondimento cito/microistologico, Comunicazione diagnosi) e i tempi chirurgici (Visita chirurgica, Intervento chirurgico, Referto istologico con marcatori biologici, Visita oncologica). Per ogni fase sono stati calcolati i tempi medi/mediani rappresentati tramite box plot e giustificati gli outliers.Risultati: La latenza nella presa in carico chirurgica \ue8 legato alla complessit\ue0 degli esami preoperatori (3) (tempo mediano tra richiamo al II\ub0 livello ed intervento: 53 giorni (se unico esame pre-operatorio) vs 73 (se pi\uf9 di un esame pre-operatorio, p<0.0001), mentre rispetto ad un recente studio (4) il tempo mediano tra visita chirurgica e intervento non \ue8 aumentato per i casi con necessit\ue0 di RM (28 vs 26 giorni, p=0.13), perch\ue9 gi\ue0 programmata in fase preoperatoria. Per i casi con mastectomia sempre con ricostruzione, si registra un tempo medio dalla visita chirurgica all\u2019intervento di 7 giorni superiore rispetto alle quadrantectomie. Ulteriore criticit\ue0 \ue8 il tempo mediano tra intervento e visita oncologica (44 giorni), attribuibile in parte ad un \u201critardo\u201d nella disponibilit\ue0 dei marcatori biomolecolari (soprattutto HER2/FISH) ed in parte a rinvii dell\u2019appuntamento da parte della paziente stessa Conclusioni: Soltanto un attento monitoraggio del turnaround time dell\u2019intero percorso delle pazienti con carcinoma screening detected consente l\u2019identificazione dei punti di debolezza su cui intervenire efficacemente per garantire il rispetto degli indicatori

Multicenter Comparison of Molecular Tumor Boards in The Netherlands: Definition, Composition, Methods, and Targeted Therapy Recommendations

Background: Molecular tumor boards (MTBs) provide rational, genomics-driven, patient-tailored treatment recommendations. Worldwide, MTBs differ in terms of scope, composition, methods, and recommendations. This study aimed to assess differences in methods and agreement in treatment recommendations among MTBs from tertiary cancer referral centers in The Netherlands. Materials and Methods: MTBs from all tertiary cancer referral centers in The Netherlands were invited to participate. A survey assessing scope, value, logistics, composition, decision-making method, reporting, and registration of the MTBs was completed through on-site interviews with members from each MTB. Targeted therapy recommendations were compared using 10 anonymized cases. Participating MTBs were asked to provide a treatment recommendation in accordance with their own methods. Agreement was based on which molecular alteration(s) was considered actionable with the next line of targeted therapy. Results: Interviews with 24 members of eight MTBs revealed that all participating MTBs focused on rare or complex mutational cancer profiles, operated independently of cancer type–specific multidisciplinary teams, and consisted of at least (thoracic and/or medical) oncologists, pathologists, and clinical scientists in molecular pathology. Differences were the types of cancer discussed and the methods used to achieve a recommendation. Nevertheless, agreement among MTB recommendations, based on identified actionable molecular alteration(s), was high for the 10 evaluated cases (86%). Conclusion: MTBs associated with tertiary cancer referral centers in The Netherlands are similar in setup and reach a high agreement in recommendations for rare or complex mutational cancer profiles. We propose a “Dutch MTB model” for an optimal, collaborative, and nationally aligned MTB workflow. Implications for Practice: Interpretation of genomic analyses for optimal choice of target therapy for patients with cancer is becoming increasingly complex. A molecular tumor board (MTB) supports oncologists in rationalizing therapy options. However, there is no consensus on the most optimal setup for an MTB, which can affect the quality of recommendations. This study reveals that the eight MTBs associated with tertiary cancer referral centers in The Netherlands are similar in setup and reach a high agreement in recommendations for rare or complex mutational profiles. The Dutch MTB model is based on a collaborative and nationally aligned workflow with interinstitutional collaboration and data sharing

Tratamientos enzimatico, microondas y ultrasonido en la extracci?n de grasa de semilla de mango (Mangifera indca L.)

73 p. Recurso Electr?nicoSe evalu? el efecto de los tratamientos de ultrasonido, microondas y enzim?tico como procesamiento previo al m?todo por prensado para aumentar el rendimiento de extracci?n de la grasa de semilla de mango. Se trabajaron dos variedades de mango Kala Alphonso y Mariquita. Las muestras fueron expuestas a ultrasonido durante 1 h, con una amplitud de 100 % y ciclo de 1 s. El tratamiento con microondas se evalu? a una potencia de 100 W durante 15 y 30 min con intervalos de 5 min evitando la evaporaci?n del agua destilada. En el tratamiento con enzimas se incub? la muestra a 45?C durante 4 h. Los rendimientos se calcularon con base al contenido inicial de aceite determinados por el m?todo Soxhlet para cada variedad 12,0 % para Kala Alphonso y 11,35 % Mariquita. Los resultados mostraron que la semilla Kala Alphonso tratada con enzima present? mayor rendimiento de extracci?n con 33,36 % en comparaci?n con Mariquita (25,99 %). Con el tratamiento de ultrasonido, la variedad Kala Alphonso obtuvo el mayor rendimiento de extracci?n (16,02 %). En el tratamiento con microondas no se logr? obtener unas condiciones de procesamiento donde la exposici?n a la energ?a electromagn?tica no afectara la estructura de la semilla y se pudiera extraer grasa de semilla de mango. De igual forma se realizaron pruebas de extracci?n de componentes grasos por el m?todo de extrusi?n, donde no se obtuvo la separaci?n de la grasa de la semilla, por lo anterior puede explicarse en que este tipo de procesamiento es m?s eficaz cuando se trabaja con semillas con mayor contenido de aceite. Las pruebas de calidad determinaron que la grasa extra?da presenta caracter?sticas similares a grasas industriales, lo que permitir?a su posible futura aplicaci?n en diferentes industrias. Palabras claves: Grasa de mango, Ultrasonido, Tratamiento enzim?tico, Microondas, Extrusi?n.It was evaluated the ultrasound, microwaves and enzymatic treatment effects as previous processing to the press method in order to increase mango seed fat extraction performance. Two varieties of mango, Kala Alphonso and Mariquita, were subjected to different treatments. The samples were exposed to ultrasound waves during 1 h, with 100 % amplitude and 1 s cycle. The microwave treatment was evaluated at minimal power (100 W) during 15 and 30 min with 5 min intervals for avoiding distilled water evaporation. In the treatment with enzymes, the sample was incubated at 45?C during 4 h. The yield was analyzed based on the initial oil content measured by Soxhlet method, 12,0 % for Kala Alphonso and 11,35 % for Mariquita. The results showed that Kala Alphonso seed treated with enzyme had a higher yield with 33,36 % compared to Mariquita with 25,99 %. In the ultrasound treatment, the Kala Alphonso variety had the higher extraction performance (16,02 %). In the microwave treatment was not possible to obtain processing conditions that exposure to electromagnetic energy will not affect the seed structure and the extraction of fat mango kernel. It was also made fat extraction tests by the extrusion method, however it was not possible to obtain fat separation, this can be explained that extrusion processing is most effective with higher oil content seeds. The quality results showed that fat mango seed presented characteristics similar to other industrial fats, so it is possible used in different industries considering its high unsaturated fatty acid content. The mechanical press extraction is an efficient process for obtaining mango seed fat but it is required previous treatments, however this is a real possibility in order to add value to an industrial sub-product. Keywords: Mango fat, Ultrasound, Enzymatic treatment, Microwaves, Extrusion

Expert System Diagnose Diseases in Goats Using the Application of Certainty Factor at the Asahan Districk Livestock Service

Goats are livestock that provides many benefits for humans such as meat, milk, and skin. Goat meat is now also one of the important foodstuffs in meeting people's nutrition and is widely marketed. Livestock health is the most important factor in the cultivation of goats, therefore the presence of diseases in goats can result in economic losses because it can reduce livestock productivity and can even cause death in goats. However, most of the goat breeders still lack knowledge of disease control in goats plus the ratio of the number of experts is not balanced with the number of breeders which causes limited service facilities for a consultation so that it is increasingly difficult for breeders to identify the type of disease early on and the solutions that will be carried out next. To overcome this problem, an expert system was built that can assist farmers in diagnosing diseases in goats. This study uses the certainty factor method in solving problems that exist in goats. The data used in this study consisted of 8 disease data and 30 symptom data caused by bacteria, viruses and parasites. Each symptom has a CF value given by experts, namely veterinarians. So that a 90% percentage of the level of conformity for the system's accuracy level is obtained based on the results of expert and system validation