Antimicrobial activities of Gold nanoparticles against Salmonella typhimurium

Background and aims: One of the major problems in hospitals is resistant pathogenic bacteria to antimicrobial substances. The problem of increased costs of treatment failure and mortality rates is increasing. The aim of this study was to evaluate the antimicrobial activity of gold nanoparticles has been on Salmonella typhimurium. Methods: This cross-sectional study was performed and Salmonella typhimurium bacteria were isolated from poultry. Gold nanoparticles for business were purchased. Minimum inhibitory concentrations of gold nanoparticles in different concentrations by dilution in the wells were determined on bacteria. Susceptibility to several antibiotics was evaluated by Kirby-Bauer disk diffusion method. Results: The result of gold nanoparticles showed the highest MIC (the minimum inhibitory concentration) was 100 ppm concentration that 6 strains of them were inhibited by this concentration. The lowest MIC was 50 ppm concentration that 1 strain of Salmonella was inhibited. The highest and lowest MBC value of extract was 200 and 100 ppm, respectively. Conclusions: The results showed that gold nanoparticles have good inhibitory effect on all studied bacteria

Investigation of the presence of G354A (Cys87Tyr) mutation in osteoprotegerin gene in women with osteoporosis in Chaharmahal and Bakhtiari province

Background and aims: Osteoprotegerin (OPG) is a competitive inhibitor of the differentiation and activity of osteoclasts, which inhibits the final stages of osteoclast formation and induces its apoptosis. In addition, OPG is considered as one of the most important candidate genes in the pathogenesis of bone diseases such as osteoporosis and idiopathic hyperphosphatasia. The G354A (Cys87Tyr) mutation in the OPG gene leads to idiopathic hyperphosphatasia. This mutation is probably related to osteoporosis. The purpose of this study was to investigate the presence of G354A (Cys87Tyr) in women with osteoporosis in Chaharmahal and Bakhtiari province. Methods: In this descriptive-analytical study, the bone mineral density (BMD) of the femoral neck and lumbar spine of women referring to Shahrekord bone densitometry centers was measured by the X-ray absorptiometry technique in 2013-2014. Based on T-scores, people with osteoporosis were identified and 70 patients were enrolled in the study after receiving their consent. Finally, DNA was extracted from blood samples, amplified by polymerase chain reaction (PCR) technique, and sequenced by DNA sequencing method. Results: After DNA extraction from the blood, the quality and quantity were determined by gel electrophoresis and spectrophotometry, respectively. Then, the gene was amplified by the PCR method and the product was detected by gel electrophoresis, followed by sequencing the samples to investigate the presence of the mutation. Eventually, genotypes associated with Cys87Tyr mutation were not observed in the studied population. Conclusion: In the present study, the G354A (Cys87Tyr) mutation associated with idiopathic hyperphosphatasia was not found in women with osteoporosis. Keywords: G354A mutation, OPG gene, Osteoporosi

Medical error and under-reporting causes from the viewpoints of nursing managers : a qualitative study

Background: Patient safety as a goal can be achieved by reporting medical errors (ME); however, most errors are never reported. The aim of this study is to explore the causes of ME, and the obstacles in reporting them amongst nurses. Methods: We conducted semi-structural interviews, with 12 nursing managers in the biggest teaching hospital in southern Iran (2015-2016). The interview guide concentrated on the causes of ME and barriers in reporting them. All face-to-face interviews were recorded and transcribed verbatim and analysed using thematic analysis. Results: In this study 4 main themes were extracted for the causes of ME: personal/social characteristics, nonprofessional practice, hospital related factors/organization contextual factors, and poor management. Also, 5 main themes (such as; personal characteristics, fear from reporting, nonprofessional practices, cultural and social factors, and error surveillance system features) were obtained with regards to barriers in reporting. Conclusions: ME can be reduced by improving professional practice and better human resource management. Also, reporting errors can be increased by focusing on cultural and social factors

Assessment of tuberculosis among male prisoners in Shiraz central prison, south of Iran.

Background: Prisons play an important role in the prevalence of Tuberculosis (TB) in a region. This study aimed to determine the situation of TB in high-risk male prisoners in Shiraz central prison of Fars province in southern Iran. Methods: This cross-sectional study (June-October 2018) was conducted on male prisoners in Shiraz central prison, southern Iran. According to 4 criteria, the prisoners were determined as high-risk prisoners for TB, and para clinical tests included three sputum samples and chest radiograph were performed for them. Then, the high risk and low risk participants were compared in terms of demographic characteristics and past medical history. Results: Among 2,995 prisoners, only 108 (3.6%) had at least one of the high-risk criteria. But after performing further TB tests for these prisoners, no prisoners with TB disease were found. The high-risk prisoners were statistically older than low-risk prisoners (38.30±9.74 vs. 35.17±9.62, P=0.001). Also, the length of incarceration was statistically different in both groups (P=0.002), and drug abuse was more in high-risk group (P<0.001). Moreover, high risk prisoners used cigarettes/day more (14.87±11.55 vs. 9.71±9.09, P<0.001), but both groups were not different in term of the marital status (P=0.519), educational level (P=0.662), job (P=0.39), and nationality (P=0.342). Conclusion: Our results showed that none of the high-risk prisoners for TB had positive test. The length of incarceration, drug abuse, smoking, as well as age were more in high-risk prisoners in comparing low risk group

Burnout among Healthcare Providers of COVID-19; a Systematic Review of Epidemiology and Recommendations

Introduction: In the current systematic review, we intended to systematically review the epidemiology of burnout and the strategies and recommendations to prevent or reduce it among healthcare providers (HCPs) of COVID-19 wards, so that policymakers can make more appropriate decisions. Methods: MEDLINE (accessed from PubMed), Science Direct, and Scopus electronic databases were systematically searched in English from December 01, 2019 to August 15, 2020, using MESH terms and related keywords. After reading the title and the abstract, unrelated studies were excluded. The full texts of the studies were evaluated by authors, independently, and the quality of the studies was determined. Then, the data were extracted and reported. Results: 12 studies were included. Five studies investigated the risks factors associated with burnout; none could establish a causal relationship because of their methodology. No study examined any intervention to prevent or reduce burnout, and the provided recommendations were based on the authors\u27 experiences and opinions. None of the studies followed up the participants, and all assessments were done according to the participants\u27 self-reporting and declaration. Assessing burnout in the HCPs working in the frontline wards was performed in four studies; others evaluated burnout among all HCPs working in the regular and frontline wards. Conclusion: Paying attention to the mental health issues, reducing the workload of HCPs through adjusting their work shifts, reducing job-related stressors, and creating a healthy work environment may prevent or reduce the burnout

Adult emergency department performance in the largest teaching hospital in southern Iran: a 1.5-year cross-sectional study

Background: Emergency department (ED) is one of the most important hospital departments, with significant effects on public health. The aim of this study was to evaluate the adult ED's performance of the largest teaching hospital in southern Iran. Methods: In this retrospective cross-sectional study (March 2017-August 2018), the registered data in the Hospital Information System (HIS) were collected, and the ED’s performance was assessed based on the Iranian emergency performance index. The slopes of the trend lines were calculated for each indicator. Moreover, 2 six-month periods were compared. Results: The data of 104,081 patients were analyzed. The mean (±standard deviation) of visited patients per-month was 5,782.28 (±1258.55). The slope of the trend line was negative for all indicators, except for discharge from ED with personal responsibility. The mean duration of waiting time for the first visit by physician in each triage level slightly decreased. Comparison of the two six-month periods showed a significant difference between the visited patient (P<0.0001). The percentage of patients disposed within six hours (P<0.0001), leaving ED within 12 hours (P<0.0001), as well as the percentage of successful cardiopulmonary resuscitation (P=0.014) in the six-month period of 2018 was significantly lower. The percentage of discharge with personal responsibility significantly increased (P=0.005). Conclusion: Although the number of patients visited in this ED decreased, all indicators had dropped. However, the percentage of discharge with personal responsibility was increased. Moreover, the mean duration of waiting time for the first visit by physician slightly decreased in each triage level

Structural Analysis of the Western Part of Shotori Mountain Range; Eastern Iran

NW-SE trending Shotori range has some structural elements such as folds and fractures (including thrust and strike-slip faults), following almost a similar trend. Based on the plotted stereograms, the predominant mechanism of the region’s faults is thrust with a dextral strike slip component which implies overcoming compressive stresses in the region. On the other hand the strike-slip horizontal component of the faults may also be due to the Nayband fault activities on Shotori and Esfandiar thrust zones. The steep dip of these faults may also be due to some normal faults converted to reverse faults as a result of stress phase change from tensional to compressional. Based on planar axis perpendicular compression or stretching axis σ1= 34, 10 and σ3= 296, 38 were obtained for the study area. striogram axial surface folds dominantly show NW-SE direction, which is indicates compression axis which is equal N53, 12.Aeromagnetic data analysis revealed two magnetic lineaments with the same trends as surveyed lineaments in the field. The relatively high magnetic intensity of these lineaments could be due to basement faults in the region. With regards to obtained stereo plots, trend of the folds’ axis are parallel to the regional thrust faults and folds’ contour diagrams apply asymmetric folds with faulting in the area

Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing

This study developed a new burn wound dressing based on core-shell nanofibers that co-deliver antibiotic and antioxidant drugs. For this purpose, poly(ethylene oxide) (PEO)-chitosan (CS)/poly(D,L-lactide-co-glycolide) (PLGA) core-shell nanofibers were fabricated through co-axial electrospinning technique. Antibiotic levofloxacin (LEV) and antioxidant quercetin (QS) were incorporated into the core and shell parts of PEO-CS/PLGA nanofibers, respectively. The drugs could bond to the polymer chains through hydrogen bonding, leading to their steady release for 168 h. An in vitro drug release study showed a burst effect followed by sustained release of LEV and QS from the nanofibers due to the Fickian diffusion. The NIH 3T3 fibroblast cell viability of the drug loaded core-shell nanofibers was comparable to that in the control (tissue culture polystyrene) implying biocompatibility of the nanofibers and their cell supportive role. However, there was no significant difference in cell viability between the drug loaded and drug free core-shell nanofibers. According to in vivo experiments, PEO-CS-LEV/PLGA-QS core-shell nanofibers could accelerate the healing process of a burn wound compared to a sterile gauze. Thanks to the synergistic therapeutic effect of LEV and QS, a significantly higher wound closure rate was recorded for the drug loaded core-shell nanofibrous dressing than the drug free nanofibers and control. Conclusively, PEO-CS-LEV/PLGA-QS core-shell nanofibers were shown to be a promising wound healing material that could drive the healing cascade through local co-delivery of LEV and QS to burn wounds

Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing

This study developed a new burn wound dressing based on core-shell nanofibers that co-deliver antibiotic and antioxidant drugs. For this purpose, poly(ethylene oxide) (PEO)-chitosan (CS)/poly(D,L-lactide-co-glycolide) (PLGA) core-shell nanofibers were fabricated through co-axial electrospinning technique. Antibiotic levofloxacin (LEV) and antioxidant quercetin (QS) were incorporated into the core and shell parts of PEO-CS/PLGA nanofibers, respectively. The drugs could bond to the polymer chains through hydrogen bonding, leading to their steady release for 168 h. An in vitro drug release study showed a burst effect followed by sustained release of LEV and QS from the nanofibers due to the Fickian diffusion. The NIH 3T3 fibroblast cell viability of the drug loaded core-shell nanofibers was comparable to that in the control (tissue culture polystyrene) implying biocompatibility of the nanofibers and their cell supportive role. However, there was no significant difference in cell viability between the drug loaded and drug free core-shell nanofibers. According to in vivo experiments, PEO-CS-LEV/PLGA-QS core-shell nanofibers could accelerate the healing process of a burn wound compared to a sterile gauze. Thanks to the synergistic therapeutic effect of LEV and QS, a significantly higher wound closure rate was recorded for the drug loaded core-shell nanofibrous dressing than the drug free nanofibers and control. Conclusively, PEO-CS-LEV/PLGA-QS core-shell nanofibers were shown to be a promising wound healing material that could drive the healing cascade through local co-delivery of LEV and QS to burn wounds

Transferred Patients by Fars Province’s Helicopter Emergency Medical Service (HEMS); A 2-Years Cross-Sectional Study in Southern Iran

Objective: To investigate the patients transferred by helicopters, as well as an emergent medical services that were performed for them.Methods: In this retrospective cross-sectional study, all patients who were transferred by Fars province of Helicopter Emergency Medical Services (HEMS) to Shiraz hospitals, southern Iran (March 2017-March 2019) were investigated. Patients’ information was collected and analyzed includes age, gender, dispatch reason, trauma mechanisms, take hold of emergent medical services, as well as the air transportation time, time between dispatch from the origin hospital and starting the procedures, and patients’ outcome.Results: Eighty-three patients were enrolled with the mean±SD age of 36.9±19.47 years that 75.9% had trauma (p <0.0001). Mental status deterioration (25.3%) was the most dispatched indications. The mortality rate was 13.25% totally (11.11% in traumatic vs. 10% in non-traumatic). The mean±SD of air transportation time was significantly lower than ground transportation in both traumatic (p=0.0013) and non-traumatic (p <0.0001) patients. Also, the mean±SD of time between dispatch from the origin hospital and starting the procedures wasstatistically lower in air transportation in both traumatic (p=0.0028) and non-traumatic (p=0.0017) patients.Conclusion: Most of the patients transferred by HEMS were traumatic. The air transportation time as well as the time between dispatches from the origin hospital to the starting of the procedures were significantly lower in HEMS in comparison with ground transportation for both traumatic and non-traumatic patients