22 research outputs found
Heat inactivation of fetal bovine serum increases protein contamination of extracellular vesicles
Introduction: Extracellular vesicles (EVs) released in cell cultures are influenced by the cell culture conditions, such as the use of
fetal bovine serum (FBS). FBS contains EVs and it is usually depleted of EVs by ultracentrifugation (UC) and/or heat inactivated
(HI). Several studies have evaluated the effect of different UC protocols for FBS by evaluating both cells and EVs. However, less
is known about the effect of HI on the cells and the released EVs. The aim of this study was therefore to evaluate the effect of HI
on EV purity.
Methods: To determine the effect of heat inactivation, three different protocols were applied based on different combinations of:
1) UC at 118,000 × g for 18h and 2)HI at 56◦C for 30 min. The three conditions tested were: FBS ultracentrifuged but not heat
inactivated (no-HI), FBS heat inactivated before UC (HI-before EV-dep), and FBS heat inactivated after EV depletion (HI-after
EV-dep). The FBS was add to themedia of threemelanoma cell lines (MML1, UM22Ctr and UM22BAP1) at a final concentration
of 10%. After 72h, large and small EVs were isolated by differential UC. The EV purity was determined by protein quantity,
electron microscopy (EM) and nanoparticle tracking analysis (NTA).
Results: The protein quantity (μg/μl) of large EVs was similar in the three conditions analyzed. On the contrary for small EVs,
the protein amount was higher when the HI was performed after EV depletion as compared to HI before the UC and UC alone.
However, significantly more particles were not detected in the HI-after EV-dep which resulted in a lower purity of small EVs in
HI-after EV-dep illustrated by calculating the ratio of number of particles/μg proteins. Presence of contaminants (indicated by
strong background) was observed in EM pictures of small EVs isolated in HI-after EV-dep condition differently from large EV
samples.
Summary/Conclusion: The HI of FBS induces release of contaminating elements that end up in small EVpellets if not previously
removed
Antilisterial effect of citrus essential oils and their performance in edible film formulations
[EN] The antimicrobial activity of eight essential oils (EOs) extracted from the fruit peel of Citrus genotypes
(orange, mandarin and lemon) was evaluated against 76 strains of Listeria monocytogenes, previously
isolated from different food matrices. EOs showing the most (EO L2 and EO L8) and least (EO O3 and EO
M7) effective inhibition activities were chemically characterized by gas chromatography coupled with
mass spectrometry (GC/MS) to compare their composition. EO L2 and EO L8 were chosen to determine
the MIC and to evaluate the cell viability of the most sensitive strains (L. monocytogenes LM35 and LM69)
after 1, 2, 4 and 6 h of exposure. The effectiveness of chitosan (CH) and methylcellulose (MC) edible films,
alone and in combination with EO L2 and EO L8, was determined against LM35 and LM69 at 37 C for 0, 8
and 24 h and at 8 C for 0, 1, 3 and 7 days. In addition, the analysis of the microstructure of the films were
performed by scanning electron microscope (SEM) to evidence the interactions between the polymers
and EOs. Thirty-five and twenty-nine strains were clearly inhibited by EO L2 and EO L8, respectively,
while the other Citrus EOs showed poor (EO M1, O4, O5, O6) or minimal (EO O3 and M7) antimicrobial
activity. A total of 36 chemical volatile substances was identified by GC/MS to detect the compounds that
might play an important role in the characterization of the EOs. The chemical characterization points to
oxygenated monoterpenes as relevant compounds in inhibiting Listeria strains, since they have been
detected in lemon EOs in concentrations four/five folds higher than orange EOs. Generally, CH- and MCbased
films containing EO L2 and EO L8 showed antilisterial activities, even though, the best performances
were observed in case of CH-films at 8 C, with a major reduction up to 3 log (CFU/cm2) in case of
EO L2 incorporation. The microstructures observed by SEM suggested a better incorporation of the EOs in
CH matrix, where a higher amount of oil droplets was distinguished. Therefore, lemon EOs incorporated
into chitosan films could be an efficient tool to control Listeria monocytogenes, especially in refrigerated
applied conditions.WR was supported by the "Student Mobility for Placement - SMP" grant of the EU Life Learning Program. The authors thank the "Azienda Sperimentale Palazzelli C.R.A. - Centro di ricerca per l'agrumicoltura e le colture mediterranee Contrada Palazzelli Scordia" (CT, Italy) for providing some of the fruits used for EOs extractions.Randazzo, W.; Jiménez Belenguer, AI.; Settanni, L.; Perdones Montero, A.; Moschetti, M.; Palazzolo, E.; Guarrasi, V.... (2016). Antilisterial effect of citrus essential oils and their performance in edible film formulations. Food Control. 59:750-758. doi:10.1016/j.foodcont.2015.06.057S7507585
Tumour cell-derived small extracellular vesicles modulate macrophage immunosuppressive phenotype associated with PD-L1 expression
Introduction: Tumour-associated macrophages (TAMs) play a key role in promoting tumour progression, by exerting an immunosuppressive phenotype associated with M2 polarization and with the expression of CD204 and programmed cell death ligand 1 (PD-L1). It is well known that tumour-derived extracellular vesicles (TEVs) play a pivotal role in the tumour microenvironment, influencing TAM behaviour. The study was aimed to examine the effect of TEVs derived from colon cancer and multiple myeloma cells on macrophage functions.
Methods: Non-polarized macrophages (M0) differentiated from THP-1 cells were co-cultured, for 3 up to 48 hours, with TEVs derived from a colon cancer cell line, SW480, and multiple myeloma cell line, MM1.S. The expression of M2 and TAM markers (respectively CD163 and CD204) as well as of PD-L1 and Interleukin 6 (IL6) were evaluated at mRNA and protein level. The apoptotic rate of CD3+ T cells cocultured with TEV-treated M0 macrophages was analysed by FACS.
Results: Our results indicate that TEVs can significantly upregulate the expression of surface markers of M2-like phenotype (CD163) and TAM (CD204) as well as of PD-L1, inducing macrophages to acquire an immunosuppressive phenotype. In parallel, we found that TEVs were also able to induce a significant increase of IL6 expression at both mRNA and protein levels and to activate the STAT3 signalling pathway. Since PD-1/PD-L1 axis is involved in the inhibition of T cells, we assessed the ability of macrophages treated with TEVs to affect T cell viability. We found that CD3+ T cells co-cultured with TEVs-treated M0 showed an increase of their apoptotic rate in comparison to CD3 + T cells grown in the presence of untreated macrophages.
Summary/Conclusion: Cumulatively, these preliminary data suggest that TEVs contribute to the immunosuppressive status of TAMs, promoting tumour growth and progression.
Funding: Grant from the Fondazione AIRC per la Ricerca sul Cancro to Riccardo Alessandro (grant n° 18783)
Citral-Enriched Fraction of Lemon Essential Oil Mitigates LPS-Induced Hepatocyte Injuries
Simple Summary To date, essential oil fractions are emerging as functional compounds of interest for the food and perfume industries. The aim of this study is to evaluate the ability of citral-enriched fractions obtained from lemon essential oil (Cfr-LEO) to counteract, in healthy human hepatocytes, the activity of lipopolysaccharide (LPS), a trigger of inflammation, oxidative stress, and epithelial-mesenchymal transition. In our paper, we report that the pretreatment of hepatocytes with Cfr-LEO counteracts the effects induced by LPS. The data obtained lay the basis for the development of commercial products such as food and drink aimed at preventing or alleviating chronic conditions associated with liver dysfunction.Abstract Lemon essential oil (LEO) is known for its aromatic and healthy properties; however, less consideration is given to the biological properties of the fractions obtained from LEO. This study aims to evaluate the ability of a citral-enriched fraction obtained from LEO (Cfr-LEO) to counteract lipopolysaccharide (LPS)-mediated inflammation, oxidative stress, and epithelial-mesenchymal transition (EMT) in healthy human hepatocytes. Human immortalized hepatocytes (THLE-2 cell line) were pretreated with Cfr-LEO and subsequently exposed to LPS at various time points. We report that the pretreatment with Cfr-LEO counteracts LPS-mediated effects by inhibiting inflammation, oxidative stress, and epithelial-mesenchymal transition in THLE-2. In particular, we found that pretreatment with Cfr-LEO reduced NF-kappa B activation and the subsequent proinflammatory cytokines release, ROS production, and NRF2 and p53 expression. Furthermore, the pretreatment with Cfr-LEO showed its beneficial effect in counteracting LPS-induced EMT. Taken together, these results support Cfr-LEO application in the nutraceutical research field not only for its organoleptic properties, conferred by citral enrichment, but also for its biological activity. Our study could lay the basis for the development of foods/drinks enriched with Cfr-LEO, aimed at preventing or alleviating chronic conditions associated with liver dysfunction
Itraconazole inhibits nuclear delivery of extracellular vesicle cargo by disrupting the entry of late endosomes into the nucleoplasmic reticulum
Extracellular vesicles (EVs) are mediators of intercellular communication under bothhealthy and pathological conditions, including the induction of pro-metastatic traits,but it is not yet known how and where functional cargoes of EVs are delivered to theirtargets in host cell compartments. We have described that after endocytosis, EVsreach Rab+late endosomes and a fraction of these enter the nucleoplasmic reticu-lum and transport EV biomaterials to the host cell nucleoplasm. Their entry thereinand docking to outer nuclear membrane occur through a tripartite complex formedby the proteins VAP-A, ORP and Rab (VOR complex). Here, we report that theantifungal compound itraconazole (ICZ), but not its main metabolite hydroxy-ICZor ketoconazole, disrupts the binding of Rab to ORP–VAP-A complexes, leadingto inhibition of EV-mediated pro-metastatic morphological changes including cellmigration behaviour of colon cancer cells. With novel, smaller chemical drugs, inhi-bition of the VOR complex was maintained, although the ICZ moieties responsiblefor antifungal activity and interference with intracellular cholesterol distributionwere removed. Knowing that cancer cells hijack their microenvironment and thatEVs derived from them determine the pre-metastatic niche, small-sized inhibitors ofnuclear transfer of EV cargo into host cells could nd cancer therapeutic applications,particularly in combination with direct targeting of cancer cell
COVID-19 in rheumatic diseases in Italy: first results from the Italian registry of the Italian Society for Rheumatology (CONTROL-19)
OBJECTIVES:
Italy was one of the first countries significantly affected by the coronavirus disease 2019 (COVID-19) epidemic. The Italian Society for Rheumatology promptly launched a retrospective and anonymised data collection to monitor COVID-19 in patients with rheumatic and musculoskeletal diseases (RMDs), the CONTROL-19 surveillance database, which is part of the COVID-19 Global Rheumatology Alliance.
METHODS:
CONTROL-19 includes patients with RMDs and proven severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) updated until May 3rd 2020. In this analysis, only molecular diagnoses were included. The data collection covered demographic data, medical history (general and RMD-related), treatments and COVID-19 related features, treatments, and outcome. In this paper, we report the first descriptive data from the CONTROL-19 registry.
RESULTS:
The population of the first 232 patients (36% males) consisted mainly of elderly patients (mean age 62.2 years), who used corticosteroids (51.7%), and suffered from multi-morbidity (median comorbidities 2). Rheumatoid arthritis was the most frequent disease (34.1%), followed by spondyloarthritis (26.3%), connective tissue disease (21.1%) and vasculitis (11.2%). Most cases had an active disease (69.4%). Clinical presentation of COVID-19 was typical, with systemic symptoms (fever and asthenia) and respiratory symptoms. The overall outcome was severe, with high frequencies of hospitalisation (69.8%), respiratory support oxygen (55.7%), non-invasive ventilation (20.9%) or mechanical ventilation (7.5%), and 19% of deaths. Male patients typically manifested a worse prognosis. Immunomodulatory treatments were not significantly associated with an increased risk of intensive care unit admission/mechanical ventilation/death.
CONCLUSIONS:
Although the report mainly includes the most severe cases, its temporal and spatial trend supports the validity of the national surveillance system. More complete data are being acquired in order to both test the hypothesis that RMD patients may have a different outcome from that of the general population and determine the safety of immunomodulatory treatments
Covid-19 And Rheumatic Autoimmune Systemic Diseases: Role of Pre-Existing Lung Involvement and Ongoing Treatments
The Covid-19 pandemic may have a deleterious impact on patients with autoimmune systemic diseases (ASD) due to their deep immune-system alterations
Tumor-derived exosomes as factors that promote metastatic niche formation: evaluation of the effects induced by colon cancer derived exosomes on functional activities and structural features of Hepatocytes
Biological Properties of a Citral-Enriched Fraction of Citrus limon Essential Oil
Lemon essential oil (LEO) is a well-known flavoring agent with versatile biological activities. In the present study, we have isolated and characterized four citral-enriched fractions of winter LEO. We reported that in murine and human macrophages the pre-treatment with a mix of these fractions (Cfr-LEO) reduces the expression of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 induced by LPS. In addition, Cfr-LEO counteracts LPS-induced oxidative stress, as shown by the increase in the GSH/GSSG ratio in comparison to cells treated with LPS alone. Overall, the results reported here encourage the application of EO fractions, enriched in citral, in the nutraceutical industry, not only for its organoleptic properties but also for its protective action against inflammation and oxidative stress
Effect of Tapentadol on Splenic Cytokine Production in Mice
BACKGROUND:: Opioid drugs affect immunity, but not all opioid drugs share the same immunomodulatory properties. Tapentadol is an analgesic drug with a dual synergistic mechanism of action: µ-opioid receptor agonism and noradrenaline reuptake inhibition. Weaker µ-opioid receptor agonism combined with noradrenaline reuptake inhibition results in potent analgesia with reduced opioid side effects. We evaluated the impact of tapentadol on splenic cytokine in normal and in hyperalgesia/allodynia mice, comparing it with morphine and reboxetine, a noradrenaline reuptake inhibitor. METHODS:: Tapentadol, reboxetine, and morphine were injected subcutaneously into naïve and mice that underwent sciatic nerve chronic constriction injury, and their effect on splenic cytokines (interferon-γ [IFN-γ], interleukin [IL]-2, IL-10, and IL-4) was measured by enzyme-linked immunosorbent assay after acute or chronic treatment. Nociceptive thresholds, thermal hyperalgesia, and allodynia also were assessed. Data were analyzed with 2-way analysis of variance (behavior) or 1-way analysis of variance (cytokines) followed by Bonferroni post hoc test. RESULTS:: Primary outcomes of our study were the effects of drugs on splenic cytokines. Our data indicate that acute tapentadol did not modify cytokine production in comparison with animals that received saline, whereas morphine suppressed all the cytokines: saline versus morphine 10 mg/kg (mean difference [MD], 95% confidence interval [CI]: IFN-γ = 12,400 [7760, 17,040], P < .001; IL-2 = 216.2 [47.69, 384.7], P < .01; IL-10 = 868 [523.7, 1212], P < .001; and IL-4 = 17.26 [10.32, 24.20], P < .001). A significant difference also was present between morphine and tapentadol (morphine 10 mg/kg versus tapentadol 20 mg/kg: MD [95% CI]: IFN-γ = −11,600 [−16,240, −6960], P < .001; IL-2 = −334.2 [−502.7, −165.7], P < .001; IL-10 = −959 [−1303, −614.7], P < .001; IL-4 = −18.66 [−25.60, −11.72], P < .001). When chronically injected for 7 days, tapentadol and reboxetine did not significantly affect cytokines when compared with saline-treated animals. The immunoprofile of tapentadol was different from that of morphine also in mice that were in a condition of neuropathic pain. All cytokines appeared significantly decreased in mice that received a chronic constriction injury in comparison with sham animals but, after 7 days of treatment, with a similar antihyperalgesic profile, IL-10 and IL-4 were significantly increased in tapentadol and reboxetine animals in comparison with morphine mice (morphine versus tapentadol: MD [95% CI], IL-10 = −926.4 [−1664, −188.5], P < .01; IL-4 = −8.15 [−12.46, −3.84], P < .001). CONCLUSIONS:: Acute and chronic tapentadol seem to be protective of splenic cytokines in contrast with morphine, which exerts a generalized suppression on all cytokines