[EN] The antimicrobial activity of eight essential oils (EOs) extracted from the fruit peel of Citrus genotypes
(orange, mandarin and lemon) was evaluated against 76 strains of Listeria monocytogenes, previously
isolated from different food matrices. EOs showing the most (EO L2 and EO L8) and least (EO O3 and EO
M7) effective inhibition activities were chemically characterized by gas chromatography coupled with
mass spectrometry (GC/MS) to compare their composition. EO L2 and EO L8 were chosen to determine
the MIC and to evaluate the cell viability of the most sensitive strains (L. monocytogenes LM35 and LM69)
after 1, 2, 4 and 6 h of exposure. The effectiveness of chitosan (CH) and methylcellulose (MC) edible films,
alone and in combination with EO L2 and EO L8, was determined against LM35 and LM69 at 37 C for 0, 8
and 24 h and at 8 C for 0, 1, 3 and 7 days. In addition, the analysis of the microstructure of the films were
performed by scanning electron microscope (SEM) to evidence the interactions between the polymers
and EOs. Thirty-five and twenty-nine strains were clearly inhibited by EO L2 and EO L8, respectively,
while the other Citrus EOs showed poor (EO M1, O4, O5, O6) or minimal (EO O3 and M7) antimicrobial
activity. A total of 36 chemical volatile substances was identified by GC/MS to detect the compounds that
might play an important role in the characterization of the EOs. The chemical characterization points to
oxygenated monoterpenes as relevant compounds in inhibiting Listeria strains, since they have been
detected in lemon EOs in concentrations four/five folds higher than orange EOs. Generally, CH- and MCbased
films containing EO L2 and EO L8 showed antilisterial activities, even though, the best performances
were observed in case of CH-films at 8 C, with a major reduction up to 3 log (CFU/cm2) in case of
EO L2 incorporation. The microstructures observed by SEM suggested a better incorporation of the EOs in
CH matrix, where a higher amount of oil droplets was distinguished. Therefore, lemon EOs incorporated
into chitosan films could be an efficient tool to control Listeria monocytogenes, especially in refrigerated
applied conditions.WR was supported by the "Student Mobility for Placement - SMP" grant of the EU Life Learning Program. The authors thank the "Azienda Sperimentale Palazzelli C.R.A. - Centro di ricerca per l'agrumicoltura e le colture mediterranee Contrada Palazzelli Scordia" (CT, Italy) for providing some of the fruits used for EOs extractions.Randazzo, W.; Jiménez Belenguer, AI.; Settanni, L.; Perdones Montero, A.; Moschetti, M.; Palazzolo, E.; Guarrasi, V.... (2016). Antilisterial effect of citrus essential oils and their performance in edible film formulations. Food Control. 59:750-758. doi:10.1016/j.foodcont.2015.06.057S7507585
