Interaction of ARC and Daxx: a novel endogenous target to preserve motor function and cell loss after focal brain ischemia in mice

The aim of this study was to explore the signaling and neuroprotective effect of transactivator of transcription (TAT) protein transduction of the apoptosis repressor with CARD (ARC) in in vitro and in vivo models of cerebral ischemia in mice. In mice, transient focal cerebral ischemia reduced endogenousARCprotein in neurons in the ischemic striatum at early reperfusion time points, and in primary neuronal cultures, RNA interference resulted in greater neuronal susceptibility to oxygen glucose deprivation (OGD).TAT.ARC protein delivery led to a dose-dependent better survival after OGD. Infarct sizes 72 h after 60 min middle cerebral artery occlusion (MCAo) were on average 30±8% (mean±SD; p=0.005; T2-weighted MRI) smaller in TAT.ARC-treated mice (1ug intraventricularly during MCAo) compared with controls. TAT.ARC-treated mice showed better performance in the pole test compared with TAT.β-Gal-treated controls. Importantly, post-stroke treatment (3 h after MCAo) was still effective in affording reduced lesion volume by 20±7% (mean±SD; p˃0.05) and better functional outcome compared with controls. Delayed treatment in mice subjected to 30 min MCAo led to sustained neuroprotection and functional behavior benefits for at least 28 d. Functionally, TAT.ARC treatment inhibited DAXX–ASK1–JNK signaling in the ischemic brain. ARC interacts with DAXX in a CARD-dependent manner to block DAXX trafficking and ASK1–JNK activation. Our work identifies for the first time ARC–DAXX binding to block ASK1–JNK activation as an ARC-specific endogenous mechanism that interferes with neuronal cell death and ischemic brain injury. Delayed delivery of TAT.ARC may present a promising target for stroke therapy

La chorégraphie de Présence de Zimmermann par John Cranko – Quelques réflexions sur les relations entre musique et danse

Mes réflexions partent de l’expérience esthétique faite en 2007 lors d’un séminaire sur John Cranko que j’avais organisé avec Claudia Jeschke à l’Université de Salzbourg et qui m’a beaucoup irrité. Ce séminaire était consacré à l’étude intensive de toute une série de chorégraphies de Cranko, incluant ses créations les plus célèbres : Romeo et Juliette d’après la musique de ballet éponyme de Serge Prokofiev (1962), Onéguine d’après de la musique instrumentale de Piotr Tchaïkovski (1965) et La ..

« Entre Denges et Denezy… ». La musique du XXe siècle en Suisse. Manuscrits et documents

traduction de dix chapitres, soit 74 page

"Alla ricerca di luce e chiarezza". L'epistolario Helmut Lachenmann - Luigi Nono (1957-1990)

Carteggio inedito completo Luigi Nono - Helmut Lachenman

Ästhetik und Komposition. Zur Aktualität der Darmstädter Ferienkursarbeit

Raccolta di saggi scaturiti dai seminari degli Internationale Ferienkurse für Neue Musik di Darmstadt degli anni 1990 e 1992