Training in Honey Bee Veterinary Medicine in Italy: An Observational Study and Practical Proposals to Face Professional Challenges

Honey bees, like other livestock, may be affected by infectious, parasitic, and abiotic diseases that need proper sanitary monitoring and control. Currently, there are limited opportunities for undergraduate students to receive education in Honey Bee Veterinary Medicine (HBVM) as part of their regular degree program, despite the professional requirements for veterinarians to carry out the increasing tasks related to honey bee health and production. Additionally, postgraduate training and specialization in HBVM is also underdeveloped. This study was an observational survey that evaluated the educational opportunities available in HBVM for current and future veterinarians in Italy. The survey analyzed both undergraduate and postgraduate programs, including Undergraduate Degree Programs in Veterinary Medicine (UDPVM), “Scuole di Specializzazione”, Masters, and other postgraduate courses. The results indicate that the current training available for veterinarians in the field of apiculture, both before and after graduation, is also insufficient in Italy, as already reported in other EU- and extra-EU countries. Finally, a roadmap for veterinary training in HBVM is developed here describing objectives and teachings aimed at fulfilling the needs of the profession in the field of beekeeping, considering the existing rules and regulations governing public health and possible evolution of this legal framework in the futur

Loss of cd45 protein in canine small clear cell/t-zone lymphoma is due to absence of gene transcription.

Canine small clear cell/T-zone lymphoma (TZL) is a peculiar lymphoma subtype characterized by an indolent clinical course and aberrant CD45-negative phenotype, easily recognized by flow cytometry (FC). Recent studies have described clinical presentation and behavior, but to date the mechanisms for CD45-negativity have never been investigated.Aim of this study is to confirm the lack of surface protein using a different technique from FC and to investigate if CD45-absence in TZL is linked to the lack of the corresponding transcript and gene.40 TZL cases and 17 controls (7 T-high grade lymphoma, 10 reactive lymphnodes) were included in the present study. Immunohistochemistry was performed with a different antibody respect with FC to confirm CD45 surface protein absence. Total RNA and genomic DNA were extracted from lymph-nodes aspirates. CD45 transcript amount was investigated by quantitative real-time RT-PCR and the corresponding gene fragment was analyzed by quantitative real-time PCR. ΔΔCt method was used for the relative quantification of transcript amount and DNA load compared to housekeeping genes.All TZL cases were negative for CD45 at immunohistochemistry. CD45 transcript amount was significantly lower in TZL compared to controls (p=0.000). This difference was not significant (p=0.584) for CD45 DNA load, that was similar between TZL and controls.These results highlight that CD45 protein is lacking on cell surface and gene transcription is absent in TZL, whereas the corresponding gene is not deleted. The data here reported support further studies for clarifying possible genomic or epigenomic factors involving CD45 gene transcription and for better clarifying the possible role of CD45 in lymphomagenesis

Veterinari i pčelarstvo: Koje su uloge, očekivanja i perspektive u budućnosti? – pregledni rad.

Apiculture is an important economy sector, facing a real health crisis. Honeybees, as well as other insect pollinator populations have been in decline over recent decades, and diseases are one of the principal challenges. To face this situation, public and private veterinary services are needed to manage surveillance, control, eradication and prevention measures of honeybee diseases within their territories, in close collaboration with beekeepers and farmers. It is necessary urgently to clarify the current problems and threats to apiculture in the public health sector. Due to possible interactions between bee health and production, the negative effects of environmental xenobiotics and climate change, this field must be considered as an interdisciplinary research issue. Moreover, honeybee veterinary medicine is increasingly engaged in the One Health approach, due to the possibility that the poor health of honeybee colonies affects human life. This review identified the key veterinarian roles, gaps in their higher education curriculum, as well as the necessity for research linkage between expectations and professional perspectives.Pčelarstvo je važan sektor gospodarstva koji se suočava sa zdravstvenom krizom. Zajednice medonosne pčele, kao i populacije drugih kukaca oprašivača se smanjuju posljednjih desetljeća. Bolesti se smatraju jednim od glavnih uzroka gubitaka u pčelarstvu. Kako bi se suočili s tim problemom potrebno je povoditi veterinarski nadzor, kontroliranje, suzbijanje i preveniranje bolesti pčela uključivanjem javnih i privatnih veterinarskih organizacija na području njihova djelovanja i u bliskoj suradnji s pčelarima i poljoprivrednicima. Nužno je potrebno osvijestiti aktualnu problematiku i ugroženost pčelarstva u javnozdravstvenom sektoru. Zbog mogućih negativnih učinaka ksenobiotika iz okoliša i klimatskih promjena na zdravlje i proizvodnju pčelinjih zajednica ovo područje zahtjeva provedbu intradisciplinarnih istraživanja. Štoviše, veterinarska medicina u području zdravlja zajednica medonosne pčele zauzima iznimno važan pristup Jednog zdravlja upravo zbog mogućnosti da narušeno zdravlje pčelinjih zajednica može negativno utjecati na zdravlje ljudi. U ovom radu istaknute su glavne uloge veterinara u pčelarstvu, nedostatci u kurikulima studijskih programa veterinarske medicine visokoobrazovnih ustanova i nužnosti provedbe istraživanja koja povezuju očekivanja i stručne perspektive

Compressor station facility failure modes: causes, taxonomy and effects

This report addresses the failure modes of compressor stations as key facilities of gas transmission networks. This is done by finding possible failure causes, establishing a taxonomy and identifying possible effects. Given the interest of this study to analyse and identify potential critical facilities in gas transmission networks, and their possible impact on energy security, loss of capacity (partial or total) has been identified as the effect of interest of the different failures. Probabilistic results provided are unavailability, expected number of failures per year, downtime and average downtime.JRC.C.3-Energy Security, Distribution and Market

GIS modeling for canine dirofilariosis risk assessment in central Italy

A survey was conducted in an area of central Italy in order to study the prevalence of Dirofilaria immitis and D. repens in dogs. Blood samples were collected from 283 dogs and examined using a modified Knott’s technique. In addition, in order to detect D. immitis occult infection, 203 serum samples were also analysed for D. immitis antigen detection. The results were analyzed in order to evaluate the behavioural and attitudinal risk factors. A geographical information system (GIS) for the study area was constructed, utilizing the following data layers: administrative boundaries, elevation, temperature, rainfall and humidity. Microfilariae were detected in 32 of the 283 dogs surveyed, constituting a total Dirofilaria prevalence of 11.3%. In particular, 20 dogs (7.1%) were positive for D. immitis and 12 dogs (4.2%) for D. repens microfilariae. One case of D. immitis occult infection was also detected. Choroplethic municipal maps were drawn within the GIS in order to display the distribution of each Dirofilaria species in the study area. Statistical analysis showed a significant association between Dirofilaria infection and animal attitude (hunting/truffle dogs showed a higher prevalence compared to guard/pet dogs). A higher prevalence was also recorded in 2 to 5-years old dogs. Furthermore a GIS-based modelling of climatic data, collected from 5 meteorological stations in the study area, was performed to estimate the yearly number of D. immitis generations in the mosquito vector. The results of the model as depicted by GIS analysis was highly concordant with the territorial distribution of positive dogs and showed that D. immitis spreading is markedly influenced by season. The potential transmission period in the study area was found to be confined to summer months with a peak in July and August, as expected for a temperate region where summer season is the most favourable period for the parasit

Effect of queen cell size on morphometric characteristics of queen honey bees (Apis mellifera ligustica)

Rearing techniques are important to determine a successful honey bee production. Queen cell cup size may affect the acceptance rate of grafted larvae and queen’s size, which in turn may influence the quality of the colonies. The present study compared the effect of different queen cell cup sizes (8.0 vs 9.0 mm diameter) on morphometric characteristics of queen honey bees. Sixty-five larvae were grafted to each treatment. Larvae transfer was carried out five times, in June and August 2019. Head, thorax and abdomen width of the newly emerged queens were measured using an electronic calliper, and the weight of each of the three segments was recorded using a precision scale. All morphometric traits measured on the accepted larvae were significantly (p < .001) higher in queens raised in larger cell cups, except for head width. Principal Component Analysis on morphometric traits shows higher values on PC1 (58.4% of explained variance) for queens raised in larger cell cups. Highest loadings were found for variables related to weight of the three parts. Among the many factors affecting queen’s quality, cell cup size seems to have a positive effect on queens’ body parts weights.HIGHLIGHTS Increasing the standard diameter of conventional cell cups allows rearing heavier and larger honey bee queens. The grafting period affects morphometric parameters, with higher size and body weight in the last decade of June

“Candidatus Midichloria” Endosymbionts Bloom after the Blood Meal of the Host, the Hard Tick Ixodes ricinus▿

“Candidatus Midichloria mitochondrii,” an intracellular symbiont of the tick Ixodes ricinus, is the only described organism able to invade the mitochondria of any multicellular organism. We used quantitative PCR to examine cycles of bacterial growth and death throughout the host's development and found that they correspond with the phases of engorgement and molt, respectively

Wolbachia and its influence on the pathology and immunology of Dirofilaria immitis infection

Since the definitive identification in 1995 of the bacterial endosymbiont Wolbachia that resides in different tissues of the filarial worm Dirofilaria immitis, there has been increasing interest to understand whether and what role it plays in the pathogenesis of and immune response to heartworm infection. The present study evaluated the effects of treatments on lung pathology in 20 beagle dogs experimentally infected with D. immitis. Dogs in Group 1 were treated with doxycycline (10 mg/kg/day) orally from weeks 0–6, 10–12, 16–18, 22–26, and 28–34. Dogs in Group 2 served as infected, non-treated controls. Dogs in Group 3 were given doxycycline as described for Group 1 combined with weekly oral doses of ivermectin (6 mcg/kg) for 34 weeks and intramuscular (IM) melarsomine (2.5 mg/kg) at week 24, followed by two additional melarsomine injections 24 h apart 1 month later. Group 4 received only melarsomine as described for Group 3. Lung lesion criteria, scored by two independent blinded pathologists, included perivascular inflammation and endothelial proliferation. Doxycycline treatment alone had no effect on lesion scores, whereas the combination of doxycycline and ivermectin resulted in less severe perivascular inflammation. All lungs were evaluated for positive immunostaining for the Wolbachia surface protein (WSP). Control dogs showed numerous thrombi, intense perivascular and interstitial inflammation and, occasionally, positive staining for WSP. Interestingly, dogs receiving doxycycline/ivermectin/melarsomine showed significantly less severe arterial lesions and the virtual absence of thrombi

Spatial distance between sites of sampling associated with genetic variation among Neospora caninum in aborted bovine foetuses from northern Italy

Background!#!Neospora caninum, a coccidian protozoan, represents an important cause of bovine abortion. Available N. caninum strains show considerable variation in vitro and in vivo, including different virulence in cattle. To which extent sexual recombination, which is possible in the intestines of domestic dogs and closely related carnivores as definitive hosts, contributes to this variation is not clear yet.!##!Methods!#!Aborted bovine foetuses were collected between 2015 and early 2019 from Italian Holstein Friesian dairy herds suffering from reproductive problems. A total of 198 samples were collected from 165 intensive farms located in Lombardy, northern Italy. N. caninum samples were subjected to multilocus-microsatellite genotyping using ten previously established microsatellite markers. In addition to our own data, those from a recent study providing data on five markers from other northern Italian regions were included and analysed.!##!Results!#!Of the 55 samples finally subjected to genotyping, 35 were typed at all or 9 out of 10 loci and their individual multilocus-microsatellite genotype (MLMG) determined. Linear regression revealed a statistically significant association between the spatial distance of the sampling sites with the genetic distance of N. caninum MLMGs (P &amp;lt; 0.001). Including data from this and a previous North Italian study into eBURST analysis revealed that several of N. caninum MLMGs from northern Italy separate into four groups; most of the samples from Lombardy clustered in one of these groups. Principle component analysis revealed similar clusters and confirmed MLMG groups identified by eBURST. Variations observed between MLMGs were not equally distributed over all loci, but predominantly observed in MS7, MS6A, or MS10.!##!Conclusions!#!Our findings confirm the concept of local N. caninum subpopulations. The geographic distance of sampling was associated with the genetic distance as determined by microsatellite typing. Results suggest that multi-parental recombination in N. caninum is a rare event, but does not exclude uniparental mating. More comprehensive studies on microsatellites in N. caninum and related species like Toxoplasma gondii should be undertaken, not only to improve genotyping capabilities, but also to understand possible functions of these regions in the genomes of these parasites

Additional file 2: Table S2. of Identification of Ixodes ricinus blood meals using an automated protocol with high resolution melting analysis (HRMA) reveals the importance of domestic dogs as larval tick hosts in Italian alpine forests

BLASTn search results and alignment for Ovis spp. amplicons obtained with Cervidae primer set. (PDF 323 kb