Phylogenetic Analyses of Two Mitochondrial Metabolic Genes Sampled in Parallel from Angiosperms Find Fundamental Interlocus Incongruence

Plant molecular phylogeneticists have supported an analytical approach of combining loci from different genomes, but the combination of mitochondrial sequences with chloroplast and nuclear sequences is potentially problematic. Low substitution rates in mitochondrial genes should decrease saturation, which is especially useful for the study of deep divergences. However, individual mitochondrial loci are insuffi ciently informative, so that combining congruent loci is necessary. For this study atp1 and cox1 were selected, which are of similar lengths, encode components of the respiratory pathway, and generally lack introns. Thus, these genes might be expected to have similar functional constraints, selection pressures, and evolutionary histories. Strictly parallel sampling of 52 species was achieved as well as six additional composite terminals with representatives from the major angiosperm clades. However, analyses of the separate loci produced strongly incongruent topologies. The source of the incongruence was investigated by validating sequences with questionable affi nities, excluding RNA-edited nucleotides, deleting taxa with unexpected phylogenetic associations, and comparing different phylogenetic methods. However, even after potential artifacts were addressed and sites and taxa putatively associated with confl ict were excluded, the resulting gene trees for the two mitochondrial loci were still substantially incongruent by all measures examined. Therefore, combining these loci in phylogenetic analysis may be counterproductive to the goal of fully resolving the angiosperm phylogeny

CCR7 is required for the in vivo function of CD4+ CD25+ regulatory T cells

CCR7-mediated migration of naive T cells into the secondary lymphoid organs is a prerequisite for their encounter with mature dendritic cells, the productive presentation of cognate antigen, and consequent T cell proliferation and effector differentiation. Therefore, CCR7 was suggested to play an important role in the initiation of adaptive immune responses. In this study, we show that primary immunity can also develop in the absence of CCR7. Moreover, CCR7-deficient knockout (KO) mice display augmented immune responses. Our data cumulatively suggest that enhanced immunity in CCR7 KO mice is caused by the defective lymph node (LN) positioning of FoxP3(+) CD4(+) CD25(+) regulatory T cells (T reg cells) and the consequent impediment of their function. The FoxP3(+) T reg cells express CCR7 and, after their adoptive transfer, migrate into the LNs of wild-type mice. Here, they proliferate in situ upon antigen stimulation and inhibit the generation of antigen-specific T cells. Conversely, transferred CCR7-deficient T reg cells fail to migrate into the LNs and suppress antigen-induced T cell responses. The transfer of combinations of naive and T reg cells from wild-type and CCR7 KO mice into syngeneic severe combined immunodeficient mice directly demonstrates that CCR7-deficient T reg cells are less effective than their wild-type counterparts in preventing the development of inflammatory bowel diseas

2. Survey Of Urban Blood Donors And Rural Populations

A CAJM survey on the prevalence of Australia Antigen in the blood of Zimbabweans (formerly Rhodesians)Consequently a more comprehensive survey was undertaken to determine the general prevalence of the antigen in Rhodesia by testing African and European blood donors as examples of urban populations and, in contrast, certain African tribal groups as examples of rural populations. The results are presented here together with the findings in cases of hepatitis and leprosy which have come to hand subsequent to the pilot survey

Preventie van preferente stroming in de zandgrond van een golfbaan

Veel zandbovengronden met een grasvegetatie hebben waterafstotende eigenschappen. Deze hydrofobe eigenschappen komen naar voren als het vochtgehalte van de grond beneden een kritieke grens daalt. Na het bereiken van deze grens zal de infiltratiesnelheid van neerslag en beregeningswater sterk afnemen. De indringing van het water gaat dan ongelijkmatig en er ontstaan preferente stroombanen in de grond. Op golfbaan "De Pan" in Bosch en Duin onderzochten we de effecten van het toedienen van een surfactant op de bevochtiging en de variatie van het vochtgehalte van de toplaag in een fairwa

Superconductivity and non-metallicity induced by doping the topological insulators Bi2Se3 and Bi2Te3

We show that by Ca-doping the Bi2Se3 topological insulator, the Fermi level can be fine tuned to fall inside the band gap and therefore suppress the bulk conductivity. Non-metallic Bi2Se3 crystals are obtained. On the other hand, the Bi2Se3 topological insulator can also be induced to become a bulk superconductor, with Tc ~ 3.8 K, by copper intercalation in the van der Waals gaps between the Bi2Se3 layers. Likewise, an as-grown crystal of metallic Bi2Te3 can be turned into a non-metallic crystal by slight variation of the Te content. The Bi2Te3 topological insulator shows small amounts of superconductivity with Tc ~ 5.5 K when reacted with Pd to form materials of the type PdxBi2Te3

A reflective conversation with Kobus Maree, Faculty of Education, University of Pretoria, South Africa

Always regarded as somewhat of an ‘outsider’ (the child of an Englishspeaking (Catholic) mother and an Afrikaans (Protestant) father in an exclusively Afrikaans milieu) and growing up extremely poor, seeing the hardship of others and realising howmuch talentwas going to waste, Kobus Maree took a particular interest in gifted disadvantaged persons. A marginalised loner, he almost inevitably developed creative abilities and took a keen interest in creativity, giftedness and the education of gifted, disadvantaged learners. As an adult, his research showed that many teachers in South Africa have to contend with the generally poor socioeconomic background of learners. A dire need for appropriate teacher and learner support materials, and school environments that are not conducive to achievement (including inadequate facilities, overcrowded classrooms, lack of teacher and learner support materials). SouthAfrica is at a critical stage in its education. It is therefore important for educators to teach emotional intelligence in their classrooms. Our biggest challenge will be tomaintain and enhance vitality in gifted education in a dynamic, ever-evolving environment. A combination of scholarly leadership and strategic management to support gifted learners is important. We should do all we can to promote societal transformation and diversity, focussing anew on underrepresented groups (women and ethnic groups) who show promise and support them. The widest array of partners possible including the big institutional players, the entire teaching fraternity (including government departments), nongovernmental organisations and miscellaneous interest groups together should develop strategic, rolling five-year plans and make gifted education a priority.http://gei.sagepub.comam201

Spatial repellents: from discovery and development to evidence based validation

International public health workers are challenged by a burden of arthropod-borne disease that remains elevated despite best efforts in control programmes. With this challenge comes the opportunity to develop novel vector control paradigms to guide product development and programme implementation. The role of vector behaviour modification in disease control was first highlighted several decades ago but has received limited attention within the public health community. This paper presents current evidence highlighting the value of sub-lethal agents, specifically spatial repellents, and their use in global health, and identifies the primary challenges towards establishing a clearly defined and recommended role for spatial repellent products in disease control

Onset of entanglement

We have developed a theory of polymer entanglement using an extended Cahn-Hilliard functional, with two extra terms. One is a nonlocal attractive term, operating over mesoscales, which is interpreted as giving rise to entanglement, and the other a local repulsive term indicative of excluded volume interactions. We show how such a functional can be derived using notions from gauge theory. We go beyond the Gaussian approximation, to the one-loop level, to show that the system exhibits a crossover to a state of entanglement as the average chain length between points of entanglement decreases. This crossover is marked by critical slowing down, as the effective diffusion constant goes to zero. We have also computed the tensile modulus of the system, and we find a corresponding crossover to a regime of high modulus.Comment: 18 pages, with 4 figure

Effective Theoretical Approach to Back Reaction of the Dynamical Casimir Effect in 1+1 Dimensions

We present an approach to studying the Casimir effects by means of the effective theory. An essential point of our approach is replacing the mirror separation into the size of space S^1 in the adiabatic approximation. It is natural to identify the size of space S^1 with the scale factor of the Robertson-Walker-type metric. This replacement simplifies the construction of a class of effective models to study the Casimir effects. To check the validity of this replacement we construct a model for a scalar field coupling to the two-dimensional gravity and calculate the Casimir effects by the effective action for the variable scale factor. Our effective action consists of the classical kinetic term of the mirror separation and the quantum correction derived by the path-integral method. The quantum correction naturally contains both the Casimir energy term and the back-reaction term of the dynamical Casimir effect, the latter of which is expressed by the conformal anomaly. The resultant effective action describes the dynamical vacuum pressure, i.e., the dynamical Casimir force. We confirm that the force depends on the relative velocity of the mirrors, and that it is always attractive and stronger than the static Casimir force within the adiabatic approximation.Comment: Published Version, 16 pages, LaTeX2e with graphics package, 1 figur

Hull Consistency Under Monotonicity

International audienceWe prove that hull consistency for a system of equations or inequalities can be achieved in polynomial time providing that the underlying functions are monotone with respect to each variable. This result holds including when variables have multiple occurrences in the expressions of the functions, which is usually a pitfall for interval-based contractors. For a given constraint, an optimal contractor can thus be enforced quickly under monotonicity and the practical significance of this theoretical result is illustrated on a simple example