Variability of alkaloid content in Papaver somniferum L.

A total of 300 accessions of opium poppy (Papaver somniferum L., Papaveraceae) of the IPK genebank collection from nearly all over the world were cultivated under field conditions in Gatersleben for morphological and phytochemical characterisation. Altogether 35 morphological and agronomic characters were collected for all accessions. Determination of chromosome numbers with flow cytometry showed that the accessions of subspecies setigerum are tetraploid whereas all accessions of the other subspecies are diploid. Composition and content of the five main alkaloids (morphine, codeine, thebaine, papaverine and noscapine) were analysed by high performance liquid chromatography (HPLC). Total alkaloid content varied between 683.32 and 25,034.84 μg/g dry matter (first year) and 1,799.49 and 25,338.55 μg/g dry matter in the second year of cultivation. There is a highly significant correlation between total content of alkaloids and morphine in both years (r=0.926/P=0.000; r=0.918/P=0.000). In contrast, the other four main alkaloids show less or no correlation with each other or the total alkaloid content. This analysis demonstrated that the amount and composition of the main alkaloids are highly variable. Additionally, there is no important correlation between morphological characters and alkaloid content. So it is not possible to use these characters as a prediction tool of alkaloid content during breeding process

Role of material properties and mesostructure on dynamic deformation and shear instability in Al-W granular composites

Dynamic experiments with Al-W granular/porous composites revealed qualitatively different behavior with respect to shear localization depending on bonding between Al particles. Two-dimensional numerical modeling was used to explore the mesomechanics of the large strain dynamic deformation in Al-W granular/porous composites and explain the experimentally observed differences in shear localization between composites with various mesostructures. Specifically, the bonding between the Al particles, the porosity, the roles of the relative particle sizes of Al and W, the arrangements of the W particles, and the material properties of Al were investigated using numerical calculations. It was demonstrated in simulations that the bonding between the "soft" Al particles facilitated shear localization as seen in the experiments. Numerical calculations and experiments revealed that the mechanism of the shear localization in granular composites is mainly due to the local high strain flow of "soft" Al around the "rigid" W particles causing localized damage accumulation and subsequent growth of the meso/macro shear bands/cracks. The "rigid" W particles were the major geometrical factor determining the initiation and propagation of "kinked" shear bands in the matrix of "soft" Al particles, leaving some areas free of extensive plastic deformation as observed in experiments and numerical calculations.Comment: 10 pages, 14 figures, submitted to Journal of Applied Physic

Optical Properties of Deep Ice at the South Pole - Absorption

We discuss recent measurements of the wavelength-dependent absorption coefficients in deep South Pole ice. The method uses transit time distributions of pulses from a variable-frequency laser sent between emitters and receivers embedded in the ice. At depths of 800 to 1000 m scattering is dominated by residual air bubbles, whereas absorption occurs both in ice itself and in insoluble impurities. The absorption coefficient increases approximately exponentially with wavelength in the measured interval 410 to 610 nm. At the shortest wavelength our value is about a factor 20 below previous values obtained for laboratory ice and lake ice; with increasing wavelength the discrepancy with previous measurements decreases. At around 415 to 500 nm the experimental uncertainties are small enough for us to resolve an extrinsic contribution to absorption in ice: submicron dust particles contribute by an amount that increases with depth and corresponds well with the expected increase seen near the Last Glacial Maximum in Vostok and Dome C ice cores. The laser pulse method allows remote mapping of gross structure in dust concentration as a function of depth in glacial ice.Comment: 26 pages, LaTex, Accepted for publication in Applied Optics. 9 figures, not included, available on request from [email protected]

A stock-flow cohort model of the national car fleet

Purpose Various regulatory and fiscal policy instruments are in force to reduce the amount of greenhouse gases and local pollutants emitted by private cars. The incentives operate primarily—or exclusively—on the newest generation of cars. But how fast will technological developments affecting new vehicle models penetrate into the car fleet? The speed at which the adverse effects of private car use will be mitigated through the normal vehicle renewal process, or through an accelerated one, carries considerable interest. Suitable modelling tools are needed. This paper aims to demonstrate the usefulness and flexibility of a bottom-up stock-flow modelling approach to private car fleet forecasting and policy analysis. Methods In the BIG model of the Norwegian automobile fleet, the annual stocks and flows characterising the car fleet are specified as matrices of 682 mutually exclusive and exhaustive cells, formed by cross-tabulations between 22 vehicle segments and 31 age classes. New car registrations follow from a disaggregate generic discrete choice model based on two decades of complete sales data for individual passenger car models. Results Example projections are presented onto the 2050 horizon under a low carbon fiscal policy scenario as well as a business-as-usual scenario. The fiscal policy is seen to make a large difference in terms of long term fuel consumption and CO2 emissions. Conclusions Stock-flow cohort modelling of the automobile fleet is a powerful and handy tool for policy analysis. Even quite simple and straightforward accounting relations may provide important insights into the dynamics of fleet development. It is possible to incorporate, into the stock-flow modelling framework, interesting and useful behavioural relations, explaining aggregate automobile ownership and travel demand, scrapping and survival rates, or consumer choice in the market for new cars

An in vivo culture system for human embryos using an encapsulation technology: a pilot study

BACKGROUND Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (<36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 1-2 h after ICSI, sibling oocytes were assigned to IUCS or conventional in vitro culture. The device was retrieved on Day 1, and all zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS Fertilization and embryo development were comparable in the in vitro and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (P = 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the in vitro arm. CONCLUSIONS Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT0048010

The AMANDA Neutrino Telescope and the Indirect Search for Dark Matter

With an effective telescope area of order 10^4 m^2, a threshold of ~50 GeV and a pointing accuracy of 2.5 degrees, the AMANDA detector represents the first of a new generation of high energy neutrino telescopes, reaching a scale envisaged over 25 years ago. We describe its performance, focussing on the capability to detect halo dark matter particles via their annihilation into neutrinos.Comment: Latex2.09, 16 pages, uses epsf.sty to place 15 postscript figures. Talk presented at the 3rd International Symposium on Sources and Detection of Dark Matter in the Universe (DM98), Santa Monica, California, Feb. 199