Intact Families with a Multiple Sclerotic Parent: Social and Emotional Adjustment of Parents and School-Age Children

Problem Statement. Research on children with a multiple sclerotic parent has been minimal and until recently focused only on negative aspects. This study investigated how families successfully cope with multiple sclerosis (MS) and how their school-age children are affected, positively or negatively. Methodology. This research used the case study approach with intact Caucasian families, 10 where the mother was the patient and 3 families where the father was the patient. The 20 children were ages 5 through 19. Following a clinical interview, the family members responded to the Family Adaptability and Cohesion Evaluation Scales-II, the Family Hardiness Index and the Family Crisis Oriented Personal Scales. The children responded to age-appropriate instruments including an empathy scale, the Survey of Interpersonal Value, the Piers-Harris Children\u27s Self-Concept Scale, the Human Figure Drawing, the Kinetic Family Drawing, and the Roberts Apperception Test. The schools completed the Vineland Adaptive Behavior Scales for the elementary children, and reported an IQ for the secondary children. Results. The families generally appeared balanced, and functioning and coping successfully. The children seemed to be adapting adequately and had basically well-adjusted personalities. They had very positive self-concepts, particularly regarding their physical self, behavior and happiness. They had good interpersonal relationships, and generally average and above empathy. The adolescents placed very high value on altruism, high value on being supported, and little value on independence. Anxiety and aggression were above average in the adolescents, and below average and normal, respectively, for the younger children. Across the age range, they seemed to have high depressive tendencies. Two families were found to have children with serious adjustment problems. Conclusions. Although MS creates definite stresses for a family, those who generally coped successfully before the onset of the illness continued to adapt to the new stressors. The 13 intact families in the study were coping well. The children accepted the additional responsibilities and loss of independence without apparent resentment. The children appeared to be positively and negatively affected by the parental illness. However, they appeared to be developing relatively normally, and should not be considered a problem population, but one in need of support

Exposure to Nonhuman Primates in Rural Cameroon

A high percentage of rural villagers are exposed to blood of nonhuman primates and risk acquiring infectious diseases

Protocol for Nearly Full-Length Sequencing of HIV-1 RNA from Plasma

Nearly full-length genome sequencing of HIV-1 using peripheral blood mononuclear cells (PBMC) DNA as a template for PCR is now a relatively routine laboratory procedure. However, this has not been the case when using virion RNA as the template and this has made full genome analysis of circulating viruses difficult. Therefore, a well-developed procedure for sequencing of full-length HIV-1 RNA directly from plasma was needed. Plasma from U.S. donors representing a range of viral loads (VL) was used to develop the assay. RNA was extracted from plasma and reverse-transcribed. Two or three overlapping regions were PCR amplified to cover the entire viral genome and sequenced for verification. The success of the procedure was sensitive to VL but was routinely successful for VL greater than 105 and the rate declined in proportion to the VL. While the two-amplicon strategy had an advantage of increasing the possibility of amplifying a single species of HIV-1, the three-amplicon strategy was more successful in amplifying samples with low viral loads. This protocol provides a useful tool for molecular analysis to understand the HIV epidemic and pathogenesis, as well as diagnosis, therapy and future vaccine strategies

Transmitted drug resistance, selection of resistance mutations and moderate antiretroviral efficacy in HIV-2: Analysis of the HIV-2 Belgium and Luxembourg database

BACKGROUND: Guidelines established for the treatment of HIV-1 infection and genotype interpretation do not apply for HIV-2. Data about antiretroviral (ARV) drug efficacy and resistance mutations is scarce. METHODS: Clinical data about HIV-2 infected patients in Belgium and Luxembourg were collected and the effect of ARV therapy on plasma viral load and CD4 counts were analysed. Viral RNA encoding for protease (PR) and reverse transcriptase (RT) from ARV-naive and treated patients were sequenced. RESULTS: Sixty-five HIV-2 infected patients were included in this cohort. Twenty patients were treated with 25 different ARV combinations in a total of 34 regimens and six months after the start of ARV therapy, only one third achieved viral load suppression. All of these successful regimens bar one contained protease inhibitors (PIs). Mean CD4 gains in the group of viral load suppressors and the group of patients treated with PI-containing regimens were respectively significantly higher than in the group of non-suppressors and the group of PI-sparing regimens. The most frequent mutations selected under therapy (compared to HIV-2 ROD) were V71I, L90M and I89V within PR. Within RT, they were M184V, Q151M, V111I and K65R. All of these mutations, except K65R and M184V, were also found in variable proportions in ARV-naive patients. CONCLUSION: Despite a high rate of ARV treatment failure, better virological and immunological results were achieved with PI-containing regimens. The analysis of polymorphic positions and HIV-2 specific mutations selected during therapy showed for the first time that transmission of drug resistant viruses has occurred in Belgium and Luxembourg. The high heterogeneity in ARV combinations reflects a lack of guidelines for the treatment of HIV-2 infection

On the diversity of malaria parasites in African apes and the origin of Plasmodium falciparum from Bonobos

The origin of Plasmodium falciparum, the etiological agent of the most dangerous forms of human malaria, remains controversial. Although investigations of homologous parasites in African Apes are crucial to resolve this issue, studies have been restricted to a chimpanzee parasite related to P. falciparum, P. reichenowi, for which a single isolate was available until very recently. Using PCR amplification, we detected Plasmodium parasites in blood samples from 18 of 91 individuals of the genus Pan, including six chimpanzees (three Pan troglodytes troglodytes, three Pan t. schweinfurthii) and twelve bonobos (Pan paniscus). We obtained sequences of the parasites' mitochondrial genomes and/or from two nuclear genes from 14 samples. In addition to P. reichenowi, three other hitherto unknown lineages were found in the chimpanzees. One is related to P. vivax and two to P. falciparum that are likely to belong to distinct species. In the bonobos we found P. falciparum parasites whose mitochondrial genomes indicated that they were distinct from those present in humans, and another parasite lineage related to P. malariae. Phylogenetic analyses based on this diverse set of Plasmodium parasites in African Apes shed new light on the evolutionary history of P. falciparum. The data suggested that P. falciparum did not originate from P. reichenowi of chimpanzees (Pan troglodytes), but rather evolved in bonobos (Pan paniscus), from which it subsequently colonized humans by a host-switch. Finally, our data and that of others indicated that chimpanzees and bonobos maintain malaria parasites, to which humans are susceptible, a factor of some relevance to the renewed efforts to eradicate malaria