Coronal emission from the shocked circumstellar ring of SN 1987A

High resolution spectra with UVES/VLT of SN 1987A from December 2000 until November 2005 show a number of high ionization lines from gas with velocities of roughly 350 km/s, emerging from the shocked gas formed by the ejecta-ring collision. These include coronal lines from [Fe X], [Fe XI] and [Fe XIV] which have increased by a factor of about 20 during the observed period. The evolution of the lines is similar to that of the soft X-rays, indicating that they arise in the same component. The line ratios are consistent with those expected from radiative shocks with velocity 310-390 km/s, corresponding to a shock temperature of (1.6-2.5) x 10^6 K. A fraction of the coronal emission may, however, originate in higher velocity adiabatic shocks.Comment: 11 pages, 10 figures, accepted for publication in A&

The technologies of isolation: apocalypse and self in Kurosawa Kiyoshi's Kairo

In this investigation of the Japanese film Kairo, I contemplate how the horrors present in the film relate to the issue of self, by examining a number of interlocking motifs. These include thematic foci on disease and technology which are more intimately and inwardly focused that the film's conclusion first appears to suggest. The true horror here, I argue, is ontological: centred on the self and its divorcing from the exterior world, especially founded in an increased use of and reliance on communicative technologies. I contend that these concerns are manifested in Kairo by presenting the spread of technology as disease-like, infecting the city and the individuals who are isolated and imprisoned by their urban environment. Finally, I investigate the meanings of the apocalypse, expounding how it may be read as hopeful for the future rather than indicative of failure or doom

Mechanism of Cancer Cell Death Induced by Depletion of an Essential Replication Regulator

Background: Depletion of replication factors often causes cell death in cancer cells. Depletion of Cdc7, a kinase essential for initiation of DNA replication, induces cancer cell death regardless of its p53 status, but the precise pathways of cell death induction have not been characterized. Methodology/Principal Findings: We have used the recently-developed cell cycle indicator, Fucci, to precisely characterize the cell death process induced by Cdc7 depletion. We have also generated and utilized similar fluorescent cell cycle indicators using fusion with other cell cycle regulators to analyze modes of cell death in live cells in both p53-positive and-negative backgrounds. We show that distinct cell-cycle responses are induced in p53-positive and-negative cells by Cdc7 depletion. p53-negative cells predominantly arrest temporally in G2-phase, accumulating CyclinB1 and other mitotic regulators. Prolonged arrest at G2-phase and abrupt entry into aberrant M-phase in the presence of accumulated CyclinB1 are followed by cell death at the post-mitotic state. Abrogation of cytoplasmic CyclinB1 accumulation partially decreases cell death. The ATR-MK2 pathway is responsible for sequestration of CyclinB1 with 14-3-3s protein. In contrast, p53-positive cancer cells do not accumulate CyclinB1, but appear to die mostly through entry into aberrant S-phase after Cdc7 depletion. The combination of Cdc7 inhibition with known anti-cancer agents significantly stimulates cell death effects in cancer cells in a genotype-dependent manner, providing a strategic basis for future combination therapies

FLOW PROPERTIES AND STRENGTH BEHAVIOUR OF MASONRY MORTAR INCORPORATING HIGH VOLUME FLY ASH

ABSTRACT: Masonry mortar is one of the most widely used building materials in construction as nearly all the walls made with bricks are finished with mortar. Masonry works with mortar are generally made with cement, sand and water. In this study, mortar mixes were made by replacing the masonry cement with up to 50% of fly ash at an incremental rate of 10%. Six mortar mixes with 1:4 volumetric cement-to-aggregate ratios using natural sand were made and tested for flow and strength properties. The results obtained from the tests showed that the masonry mortars manufactured with fly ash are better off as compared to the mix without any ash. In terms of flow, it was found that the addition of fly ash increased the flow, particularly when the replacement levels were above 40%. Mortar with high volume fly ash has also shown to attain a comparable strength with that of the control mix. Considering the better workability and acceptable strength properties, the results have demonstrated that high volume replacement of fly ash is a viable alternative for producing environmental friendly masonry mortar

Diagnostics of the Early Explosion Phase of a Classical Nova Using Its X-ray Emission: A Model for the X-ray Outburst of CI Camelopardalis in 1998

We have computed a spherically symmetric model for the interaction of matter ejected during the outburst of a classical nova with the stellar wind from its optical component.This model is used to describe the intense X-ray outburst (the peak 3-20 keV flux was ~2 Crab) of the binary system CI Camelopardalis in 1998. According to our model, the stellar wind from the optical component heated by a strong shock wave produced when matter is ejected from the white dwarf as the result of a thermonuclear explosion on its surface is the emission source in the standard X-ray band. Comparison of the calculated and observed time dependences of the mean radiation temperature and luminosity of the binary system during its outburst has yielded very important characteristics of the explosion.We have been able to measure the velocity of the ejected matter immediately after the onset of the explosion for the first time: it follows from our model that the ejected matter had a velocity of ~2700 km/s even on 0.1-0.5 day after the outburst onset and it flew with such a velocity for the first 1-1.5 day under an external force, possibly, the radiation pressure from the white dwarf. Subsequently, the matter probably became transparent and began to decelerate. The time dependence of the mean radiation temperature at late expansion phases has allowed us to estimate the mass of the ejected matter, ~10^{-7}-10^{-6} Msun. The mass loss rate in the stellar wind required to explain the observed peak luminosity of the binary system during its outburst has been estimated to be dM/dt ~(1-2)x10^{-6} Msun/yr.Comment: 19 pages, 24 figures; unimportant correction of the formul

Cooperation of Mtmr8 with PI3K Regulates Actin Filament Modeling and Muscle Development in Zebrafish

It has been shown that mutations in at least four myotubularin family genes (MTM1, MTMR1, 2 and 13) are causative for human neuromuscular disorders. However, the pathway and regulative mechanism remain unknown.Here, we reported a new role for Mtmr8 in neuromuscular development of zebrafish. Firstly, we cloned and characterized zebrafish Mtmr8, and revealed the expression pattern predominantly in the eye field and somites during early somitogenesis. Using morpholino knockdown, then, we observed that loss-of-function of Mtmr8 led to defects in somitogenesis. Subsequently, the possible underlying mechanism and signal pathway were examined. We first checked the Akt phosphorylation, and observed an increase of Akt phosphorylation in the morphant embryos. Furthermore, we studied the PH/G domain function within Mtmr8. Although the PH/G domain deletion by itself did not result in embryonic defect, addition of PI3K inhibitor LY294002 did give a defective phenotype in the PH/G deletion morphants, indicating that the PH/G domain was essential for Mtmr8's function. Moreover, we investigated the cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development, and found that both Mtmr8-MO1 and Mtmr8-MO2+LY294002 led to the disorganization of the actin cytoskeleton. In addition, we revealed a possible participation of Mtmr8 in the Hedgehog pathway, and cell transplantation experiments showed that Mtmr8 worked in a non-cell autonomous manner in actin modeling.The above data indicate that a conserved functional cooperation of Mtmr8 with PI3K regulates actin filament modeling and muscle development in zebrafish, and reveal a possible participation of Mtmr8 in the Hedgehog pathway. Therefore, this work provides a new clue to study the physiological function of MTM family members

Cdc7p-Dbf4p Regulates Mitotic Exit by Inhibiting Polo Kinase

Cdc7p-Dbf4p is a conserved protein kinase required for the initiation of DNA replication. The Dbf4p regulatory subunit binds Cdc7p and is essential for Cdc7p kinase activation, however, the N-terminal third of Dbf4p is dispensable for its essential replication activities. Here, we define a short N-terminal Dbf4p region that targets Cdc7p-Dbf4p kinase to Cdc5p, the single Polo kinase in budding yeast that regulates mitotic progression and cytokinesis. Dbf4p mediates an interaction with the Polo substrate-binding domain to inhibit its essential role during mitosis. Although Dbf4p does not inhibit Polo kinase activity, it nonetheless inhibits Polo-mediated activation of the mitotic exit network (MEN), presumably by altering Polo substrate targeting. In addition, although dbf4 mutants defective for interaction with Polo transit S-phase normally, they aberrantly segregate chromosomes following nuclear misorientation. Therefore, Cdc7p-Dbf4p prevents inappropriate exit from mitosis by inhibiting Polo kinase and functions in the spindle position checkpoint