Company Performance Measurement

Diplomová práce se zabývá hodnocením výkonnosti společnosti LAC, s.r.o.. Teoretická část práce seznamuje se systémem měření výkonnosti a podstatou strategické analýzy. Následující část představuje společnost, její podnikatelské činnosti a zhodnocení současného stavu prostřednictvím strategické a finanční analýzy. Na základě výstupů provedených analýz je v analytické části práce navržen koncept metody Balanced Scorecard. Závěrečná část práce přináší návrh implementace Balanced Scorecard do prostředí společnosti za účelem zvýšení její výkonnosti a dalšího rozvoje.This master´s thesis evaluates the performance of the LAC, s.r.o. company. The theoretical part of the work introduces both a system of performance measurement and also the key principles of strategic analysis. Following this there is an introduction to the company, and to its business activities, together with an evaluation of its current status, utilising strategic and financial analysis. Based on the outputs of the analyses that were carried-out, the analytical part of this thesis presents the design of the Balanced Scorecard method. The final part of the work discusses a proposal for the implementation of a Balanced Scorecard in a corporate environment, with the aim of increasing the company performance and facilitating its future development.

Evaluation of the Financial Situation of a Company and Proposals for its Improvement

Bakalářská práce se zabývá hodnocením finanční situace společnosti C SYSTEM CZ, a.s. v letech 2008 - 2012 pomocí metody finanční analýzy. Práce je rozdělena do čtyř kapitol, v první části přináší informace o společnosti, ve druhé části teoretická východiska finanční analýzy, která jsou uplatněna v následující praktické části. Vyhodnocení výsledků analýzy je náplní čtvrté kapitoly. Cílem bakalářské práce je analyzovat současnou finanční situaci společnosti a navrhnout možná zlepšení.The bachelor´s thesis deals with the evaluation of the financial situation of the company C SYSTEM CZ, a.s. within the years 2008 - 2012 on the basic of selected methods of financial analysis. The thesis is devided into four parts, the first one gives basic information about the company, the theoretical concepts of the second part is then brought into practice in the following part of the thesis. The interpretation of the results of the financial analysis is presented in the last part of the thesis. The thesis aims to evaluate the present finantial situation of the company to find proposals for its improvement.

Optimalization of preparation of apo-cytochrome b5 utilizing apo-myoglobin

Cytochrome b5 (cyt b5), a component of endoplasmic reticulum membrane, plays a role in modulation of enzymatic activity of some cytochrome P450 (CYP) enzymes. The effect of apo-cytochrome b5 on this enzymatic system has not been investigated in details, because preparation of cyt b5 as a pure protein failed in many laboratories. In order to prepare the native apo-cytochrome b5 in a large scale we utilized a protein with higher affinity toward the heme; the apo-myoglobin from the equine skeletal muscle. In the first step, we extracted heme moiety from the native myoglobin by butanone extraction. Than the effect of pH on spontaneous heme release from both proteins was investigated: purified rabbit cyt b5 as well as equine skeletal muscle myoglobin. The prepared apo-myoglobin was incubated with the cyt b5 and heme transfer was monitored as a shift of absorption maximum from 413 to 409 nm in pH varying between 3–6 (10 mM KH2PO4, pH 3–6). Here, we obtained 43 mg of the equine skeletal muscle apo-myoglobin (43% yield). The optimal pH range for heme transfer from cyt b5 into apo-myoglobin was between 4.2 and 5. Native apo-cytochrome b5 was successfully prepared using procedure described here

Genotoxic mechanisms for the carcinogenicity of the environmental pollutants and carcinogens o-anisidine and 2-nitroanisole follow from adducts generated by their metabolite N-(2-methoxyphenyl)-hydroxylamine with deoxyguanosine in DNA

An aromatic amine, o-anisidine (2-methoxyaniline) and its oxidative counterpart, 2-nitroanisole (2-methoxynitrobenzene), are the industrial and environmental pollutants causing tumors of the urinary bladder in rats and mice. Both carcinogens are activated to the same proximate carcinogenic metabolite, N-(2-methoxyphenyl)hydroxylamine, which spontaneously decomposes to nitrenium and/or carbenium ions responsible for formation of deoxyguanosine adducts in DNA in vitro and in vivo. In other words, generation of N-(2-methoxyphenyl)hydroxylamine is responsible for the genotoxic mechanisms of the o-anisidine and 2-nitroanisole carcinogenicity. Analogous enzymes of human and rat livers are capable of activating these carcinogens. Namely, human and rat cytochorme P4502E1 is the major enzyme oxidizing o-anisidine to N-(2-methoxyphenyl)hydroxylamine, while xanthine oxidase of both species reduces 2-nitroanisole to this metabolite. Likewise, O-demethylation of 2-nitroanisole, which is the detoxication pathway of its metabolism, is also catalyzed by the same human and rat enzyme, cytochorme P450 2E1. The results demonstrate that the rat is a suitable animal model mimicking the fate of both carcinogens in humans and suggest that both compounds are potential carcinogens also for humans

Ellipticine cytotoxicity to cancer cell lines — a comparative study

Ellipticine is a potent antineoplastic agent exhibiting multiple mechanisms of action. This anticancer agent should be considered a pro-drug, whose pharmacological efficiency and/or genotoxic side effects are dependent on its cytochrome P450 (CYP)- and/or peroxidase-mediated activation to species forming covalent DNA adducts. Ellipticine can also act as an inhibitor or inducer of biotransformation enzymes, thereby modulating its own metabolism leading to its genotoxic and pharmacological effects. Here, a comparison of the toxicity of ellipticine to human breast adenocarcinoma MCF-7 cells, leukemia HL-60 and CCRF-CEM cells, neuroblastoma IMR-32, UKF-NB-3 and UKF-NB-4 cells and U87MG glioblastoma cells and mechanisms of its action to these cells were evaluated. Treatment of all cells tested with ellipticine resulted in inhibition of cell growth and proliferation. This effect was associated with formation of two covalent ellipticine-derived DNA adducts, identical to those formed by 13-hydroxy- and 12-hydroxyellipticine, the ellipticine metabolites generated by CYP and peroxidase enzymes, in MCF-7, HL-60, CCRF-CEM, UKF-NB-3, UKF-NB-4 and U87MG cells, but not in neuroblastoma UKF-NB-3 cells. Therefore, DNA adduct formation in most cancer cell lines tested in this comparative study might be the predominant cause of their sensitivity to ellipticine treatment, whereas other mechanisms of ellipticine action also contribute to its cytotoxicity to neuroblastoma UKF-NB-3 cells

Cytochrome P450-mediated metabolism of N-(2-methoxyphenyl)-hydroxylamine, a human metabolite of the environmental pollutants and carcinogens o-anisidine and o-nitroanisole

N-(2-methoxyphenyl)hydroxylamine is a human metabolite of the industrial and environmental pollutants and bladder carcinogens 2-methoxyaniline (o-anisidine) and 2-methoxynitrobenzene (o-nitroanisole). Here, we investigated the ability of hepatic microsomes from rat and rabbit to metabolize this reactive compound. We found that N-(2-methoxyphenyl)hydroxylamine is metabolized by microsomes of both species mainly to o-aminophenol and a parent carcinogen, o-anisidine, whereas 2-methoxynitrosobenzene (o-nitrosoanisole) is formed as a minor metabolite. Another N-(2-methoxyphenyl)hydroxylamine metabolite, the exact structure of which has not been identified as yet, was generated by hepatic microsomes of rabbits, but its formation by those of rats was negligible. To evaluate the role of rat hepatic microsomal cytochromes P450 (CYP) in N-(2-methoxyphenyl)hydroxylamine metabolism, we investigated the modulation of its metabolism by specific inducers of these enzymes. The results of this study show that rat hepatic CYPs of a 1A subfamily and, to a lesser extent those of a 2B subfamily, catalyze N-(2-methoxyphenyl)hydroxylamine conversion to form both its reductive metabolite, o-anisidine, and o-aminophenol. CYP2E1 is the most efficient enzyme catalyzing conversion of N-(2-methoxyphenyl)hydroxylamine to o-aminophenol

Quality costs in operation of dental gas in MCHZ a.s. Ostrava

Práce se zabývá stanovením nákladů spojených s jakostí, vznikajících při výrobě rajského plynu, v MCHZ a.s. Ostrava.Dokončená práce s úspěšnou obhajobo

Labe IV:Vliv ekologických zátěží na tok Labe

Problematika průzkumu významných ekologických zátěží. Podrobný monitoring Spolany Neratovice (poloha lokality, historie a současnost lokality, hlavní ekologické zátěže areálu, sledovaný dopad činnosti na povrchové vody, modelové řešení proudění podzemní vody, vstupní údaje pro upřesnění koncepčního modelu, stacionární simulace, průzkum kontaminace polychlorovanými bifenyly a chlorovanými pesticidy. Hlavním cílem dílčího úkolu je posouzení vlivu jednotlivých ekologických zátěží na tok Labe