4 research outputs found
Contrasted breeding strategies in four sympatric sibling insect species: when a proovigenic and capital breeder copes with a stochastic environment
International audience1. The evolution of strategies of resource acquisition and allocation is often considered to be closely dependent on the degree of environmental variability. Within this framework, female insects that experience stochastic fluctuations in the availability of their egg-laying sites in time or space can be expected to be fully synovigenic (i.e. they start maturing eggs after a delay once reaching adulthood), which allows them to tailor their reproductive investment to variations in the resource. Proovigenic females (that have most of their eggs already mature at the onset of their adult life, which corresponds to a capital breeding strategy), on the contrary, should have an advantage when the availability of the egg-laying sites is predictable. There is, however, a dearth of empirical studies testing these predictions. 2. Here, we tested the hypothesis that four phytophagous insect species of the genus Curculio, which coexist on a strongly fluctuating resource that they exploit for egg-laying purposes, would all be synovigenic as strict proovigeny should be counterselected. The resource consisted of the acorns of oak trees Quercus spp. We conducted field surveys to determine the date of adult emergence in each weevil species and the ability of newly emerged females to produce eggs. We also analysed the stable isotope profile of wild-caught females as a proxy for their feeding activity. Finally, we tested females under laboratory conditions for their ability to produce mature eggs when not fed and investigated whether dietary intake influenced their longevity. 3. Taken together, our results show that, contrary to the usual predictions, the four weevil species that were all exposed to a markedly fluctuating environment exhibited sharply contrasting strategies of resource acquisition and allocation: three species were synovigenic, while the fourth was proovigenic. Unexpectedly, therefore, our findings show that a strict capital breeding species might not always be counterselected in a temporally stochastic environment. They further suggest that fluctuations in the environment should not promote a sole, optimal strategy of energy acquisition and allocation to reproduction but instead should favour their diversification
Structural basis for substrate selectivity and nucleophilic substitution mechanisms in human adenine phosphoribosyltransferase catalyzed reaction
International audienceThe reversible adenine phosphoribosyltransferase enzyme (APRT) is essential for purine homeostasis in prokaryotes and eukaryotes. In humans, APRT (hAPRT) is the only enzyme known to produce AMP in cells from dietary adenine. APRT can also process adenine analogs, which are involved in plant development or neuronal homeostasis. However, the molecular mechanism underlying substrate specificity of APRT and catalysis in both directions of the reaction remains poorly understood. Here we present the crystal structures of hAPRT complexed to three cellular nucleotide analogs (hypoxanthine, IMP, and GMP) that we compare with the phosphate-bound enzyme. We established that binding to hAPRT is substrate shape-specific in the forward reaction, whereas it is base-specific in the reverse reaction. Furthermore , a quantum mechanics/molecular mechanics (QM/ MM) analysis suggests that the forward reaction is mainly a nucleophilic substitution of type 2 (S N 2) with a mix of S N 1-type molecular mechanism. Based on our structural analysis, a magnesium-assisted S N 2-type mechanism would be involved in the reverse reaction. These results provide a framework for understanding the molecular mechanism and substrate discrimination in both directions by APRTs. This knowledge can play an instrumental role in the design of inhibitors, such as antiparasitic agents, or adenine-based substrates
Structural Insights into the Forward and Reverse Enzymatic Reactions in Human Adenine Phosphoribosyltransferase
International audienceHighlights d Human APRT catalyzes the transformation of adenine into AMP and vice versa d Complexes with substrates in both directions of the reaction highlight key residues d The catalytic flexible loop dynamic is revealed by an in crystallo activity d Tyr105 is essential for cell growth by facilitating the forward reaction In Brief APRT is a key enzyme in the purine salvage pathway in prokaryotes and eukaryotes. Huyet et al., by using in vitro, cellular, and in crystallo enzymatic analyses, reveal that a hydroxyl group in a conserved tyrosine controls the protein dynamics and the catalytic efficiencies of the forward and reverse reactions