531 research outputs found
Geometry and Dynamics of a Coupled 4D-2D Quantum Field Theory
Geometric and dynamical aspects of a coupled 4D-2D interacting quantum field
theory - the gauged nonAbelian vortex - are investigated. The fluctuations of
the internal 2D nonAbelian vortex zeromodes excite the massless 4D Yang-Mills
modes and in general give rise to divergent energies. This means that the
well-known 2D CP(N-1) zeromodes associated with a nonAbelian vortex become
nonnormalizable. Moreover, all sorts of global, topological 4D effects such as
the nonAbelian Aharonov-Bohm effect come into play. These topological global
features and the dynamical properties associated with the fluctuation of the 2D
vortex moduli modes are intimately correlated, as shown concretely here in a
U(1) x SU(N) x SU(N) model with scalar fields in a bifundamental representation
of the two SU(N) factor gauge groups.Comment: Latex, 39 pages, 5 figure
Non-Abelian discrete gauge symmetries in 4d string models
We study the realization of non-Abelian discrete gauge symmetries in 4d field
theory and string theory compactifications. The underlying structure
generalizes the Abelian case, and follows from the interplay between gaugings
of non-Abelian isometries of the scalar manifold and field identifications
making axion-like fields periodic. We present several classes of string
constructions realizing non-Abelian discrete gauge symmetries. In particular,
compactifications with torsion homology classes, where non-Abelianity arises
microscopically from the Hanany-Witten effect, or compactifications with
non-Abelian discrete isometry groups, like twisted tori. We finally focus on
the more interesting case of magnetized branes in toroidal compactifications
and quotients thereof (and their heterotic and intersecting duals), in which
the non-Abelian discrete gauge symmetries imply powerful selection rules for
Yukawa couplings of charged matter fields. In particular, in MSSM-like models
they correspond to discrete flavour symmetries constraining the quark and
lepton mass matrices, as we show in specific examples.Comment: 58 pages; minor typos corrected and references adde
Vortex deformation and breaking in superconductors: A microscopic description
Vortex breaking has been traditionally studied for nonuniform critical
current densities, although it may also appear due to nonuniform pinning force
distributions. In this article we study the case of a
high-pinning/low-pinning/high-pinning layered structure. We have developed an
elastic model for describing the deformation of a vortex in these systems in
the presence of a uniform transport current density for any arbitrary
orientation of the transport current and the magnetic field. If is above a
certain critical value, , the vortex breaks and a finite effective
resistance appears. Our model can be applied to some experimental
configurations where vortex breaking naturally exists. This is the case for
YBaCuO (YBCO) low angle grain boundaries and films on vicinal
substrates, where the breaking is experienced by Abrikosov-Josephson vortices
(AJV) and Josephson string vortices (SV), respectively. With our model, we have
experimentally extracted some intrinsic parameters of the AJV and SV, such as
the line tension and compared it to existing predictions based on
the vortex structure.Comment: 11 figures in 13 files; minor changes after printing proof
Carbogen-induced changes in rat mammary tumour oxygenation reported by near infrared spectroscopy
We have evaluated the ability of steady-state, radially-resolved, broad-band near infrared diffuse reflectance spectroscopy to measure carbogen-induced changes in haemoglobin oxygen saturation (SO2) and total haemoglobin concentration in a rat R3230 mammary adenocarcinoma model in vivo. Detectable shifts toward higher saturations were evident in all tumours (n = 16) immediately after the onset of carbogen breathing. The SO2 reached a new equilibrium within 1 min and remained approximately constant during 200–300 s of administration. The return to baseline saturation was more gradual when carbogen delivery was stopped. The degree to which carbogen increased SO2 was variable among tumours, with a tendency for tumours with lower initial SO2 to exhibit larger changes. Tumour haemoglobin concentrations at the time of peak enhancement were also variable. In the majority of cases, haemoglobin concentration decreased in response to carbogen, indicating that increased tumour blood volume was not responsible for the observed elevation in SO2. We observed no apparent relationship between the extent of the change in tumour haemoglobin concentration and the magnitude of the change in the saturation. Near infrared diffuse reflectance spectroscopy provides a rapid, non-invasive means of monitoring spatially averaged changes in tumour haemoglobin oxygen saturation induced by oxygen modifiers. © 1999 Cancer Research Campaig
Sexual Size Dimorphism and Body Condition in the Australasian Gannet
Funding: The research was financially supported by the Holsworth Wildlife Research Endowment. Acknowledgments We thank the Victorian Marine Science Consortium, Sea All Dolphin Swim, Parks Victoria, and the Point Danger Management Committee for logistical support. We are grateful for the assistance of the many field volunteers involved in the study.Peer reviewedPublisher PD
Calcium Homeostasis in Myogenic Differentiation Factor 1 (MyoD)-Transformed, Virally-Transduced, Skin-Derived Equine Myotubes
Dysfunctional skeletal muscle calcium homeostasis plays a central role in the pathophysiology of several human and animal skeletal muscle disorders, in particular, genetic disorders associated with ryanodine receptor 1 (RYR1) mutations, such as malignant hyperthermia, central core disease, multiminicore disease and certain centronuclear myopathies. In addition, aberrant skeletal muscle calcium handling is believed to play a pivotal role in the highly prevalent disorder of Thoroughbred racehorses, known as Recurrent Exertional Rhabdomyolysis. Traditionally, such defects were studied in human and equine subjects by examining the contractile responses of biopsied muscle strips exposed to caffeine, a potent RYR1 agonist. However, this test is not widely available and, due to its invasive nature, is potentially less suitable for valuable animals in training or in the human paediatric setting. Furthermore, increasingly, RYR1 gene polymorphisms (of unknown pathogenicity and significance) are being identified through next generation sequencing projects. Consequently, we have investigated a less invasive test that can be used to study calcium homeostasis in cultured, skin-derived fibroblasts that are converted to the muscle lineage by viral transduction with a MyoD (myogenic differentiation 1) transgene. Similar models have been utilised to examine calcium homeostasis in human patient cells, however, to date, there has been no detailed assessment of the cells’ calcium homeostasis, and in particular, the responses to agonists and antagonists of RYR1. Here we describe experiments conducted to assess calcium handling of the cells and examine responses to treatment with dantrolene, a drug commonly used for prophylaxis of recurrent exertional rhabdomyolysis in horses and malignant hyperthermia in humans
Phenotypic Variation and Bistable Switching in Bacteria
Microbial research generally focuses on clonal populations. However, bacterial cells with identical genotypes frequently display different phenotypes under identical conditions. This microbial cell individuality is receiving increasing attention in the literature because of its impact on cellular differentiation, survival under selective conditions, and the interaction of pathogens with their hosts. It is becoming clear that stochasticity in gene expression in conjunction with the architecture of the gene network that underlies the cellular processes can generate phenotypic variation. An important regulatory mechanism is the so-called positive feedback, in which a system reinforces its own response, for instance by stimulating the production of an activator. Bistability is an interesting and relevant phenomenon, in which two distinct subpopulations of cells showing discrete levels of gene expression coexist in a single culture. In this chapter, we address techniques and approaches used to establish phenotypic variation, and relate three well-characterized examples of bistability to the molecular mechanisms that govern these processes, with a focus on positive feedback.
The Set3/Hos2 Histone Deacetylase Complex Attenuates cAMP/PKA Signaling to Regulate Morphogenesis and Virulence of Candida albicans
Candida albicans, like other pleiomorphic fungal pathogens, is able to undergo a reversible transition between single yeast-like cells and multicellular filaments. This morphogenetic process has long been considered as a key fungal virulence factor. Here, we identify the evolutionarily conserved Set3/Hos2 histone deacetylase complex (Set3C) as a crucial repressor of the yeast-to-filament transition. Cells lacking core components of the Set3C are able to maintain all developmental phases, but are hypersusceptible to filamentation-inducing signals, because of a hyperactive cAMP/Protein Kinase A signaling pathway. Strikingly, Set3C-mediated control of filamentation is required for virulence in vivo, since set3Δ/Δ cells display strongly attenuated virulence in a mouse model of systemic infection. Importantly, the inhibition of histone deacetylase activity by trichostatin A exclusively phenocopies the absence of a functional Set3C, but not of any other histone deacetylase gene. Hence, our work supports a paradigm for manipulating morphogenesis in C. albicans through alternative antifungal therapeutic strategies
Genes Selectively Up-Regulated by Pheromone in White Cells Are Involved in Biofilm Formation in Candida albicans
To mate, MTL-homozygous strains of the yeast pathogen Candida albicans must switch from the white to opaque phase. Mating-competent opaque cells then release pheromone that induces polarization, a G1 block and conjugation tube formation in opaque cells of opposite mating type. Pheromone also induces mating-incompetent white cells to become adhesive and cohesive, and form thicker biofilms that facilitate mating. The pheromone response pathway of white cells shares the upstream components of that of opaque cells, but targets a different transcription factor. Here we demonstrate that the genes up-regulated by the pheromone in white cells are activated through a common cis-acting sequence, WPRE, which is distinct from the cis-acting sequence, OPRE, responsible for up-regulation in opaque cells. Furthermore, we find that these white-specific genes play roles in white cell biofilm formation, and are essential for biofilm formation in the absence of an added source of pheromone, suggesting either an autocrine or pheromone-independent mechanism. These results suggest an intimate, complex and unique relationship between switching, mating and MTL-homozygous white cell biofilm formation, the latter a presumed virulence factor in C. albicans
N-Acetylglucosamine Induces White to Opaque Switching, a Mating Prerequisite in Candida albicans
To mate, the fungal pathogen Candida albicans must undergo homozygosis at the mating-type locus and then switch from the white to opaque phenotype. Paradoxically, opaque cells were found to be unstable at physiological temperature, suggesting that mating had little chance of occurring in the host, the main niche of C. albicans. Recently, however, it was demonstrated that high levels of CO2, equivalent to those found in the host gastrointestinal tract and select tissues, induced the white to opaque switch at physiological temperature, providing a possible resolution to the paradox. Here, we demonstrate that a second signal, N-acetylglucosamine (GlcNAc), a monosaccharide produced primarily by gastrointestinal tract bacteria, also serves as a potent inducer of white to opaque switching and functions primarily through the Ras1/cAMP pathway and phosphorylated Wor1, the gene product of the master switch locus. Our results therefore suggest that signals produced by bacterial co-members of the gastrointestinal tract microbiota regulate switching and therefore mating of C. albicans
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