Non-Measles Hemadsorption in a Cell Line Persistently Infected with Measles Virus (BGM/MV)

Adsorption of Rhesus monkey erythrocytes to the plasma membranes of measles virus infected cells is frequently carried out to detect the presence of plasma membrane-associated measles virus hemagglutinin. The hemagglutinin is a viral genome-coded structural glycoprotein of the measles virion that is associated with the plasma membrane of the host cell during measles virus replication. BGM/MV, anon-virogenic line of African green monkey kidney cells persistently-infected with measles virus, adsorbed Rhesus monkey erythrocytes in an inverse fashion relative to the number of cells present in the culture and the time post-seeding. Serological studies employing the hemadsorption-inhibition and membrane immunofluorescence assay procedures, suggested that this phenomenon was not mediated by the viral hemagglutinin. Assays for Simian virus-5 and mycoplasma, contaminating agents that induce erythrocyte adsorption, were negative. Incubation of BGM/MV cells at 33°C or with graded concentrations of fetal calf serum, to stimulate the metabolism of resting (Go) cells, suggested that adsorption was related to a phase(s) of the cell growth cycle other than Go₁, for adsorption was prolonged and stimulated in a dose-response fashion, respectively. Comparative adsorption studies employing the parent cell line (BGM), not infected with measles virus, were performed using various species of erythrocytes. While both cell lines adsorbed Rhesus monkey erythrocytes in an inverse fashion relative to cell density, differences were noted in the adsorption of some of the other species of erythrocytes. These data suggest that Rhesus monkey erythrocyte adsorption to BGM/MV cells was mediated by a receptor(s) of cellular origin

Anti-CTLA-4 (CD 152) monoclonal antibody-induced autoimmune interstitial nephritis

Targeted immune-modulating agents are entering clinical practice in many specialties, providing novel therapeutic possibilities but introducing new potential toxicities. We present the first reported case, to our knowledge, of immune-mediated nephritis following the administration of Tremelimumab (CP-675, 206), an anti-cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) monoclonal antibody. High-dose steroid therapy led to a rapid improvement in renal function, avoiding the need for renal replacement therapy.Peer reviewe

Experimental behavior of existing RC columns strengthened with HPFRC jacket under concentric and eccentric compressive load

Reinforced concrete (RC) structures built before the 1970 represent a large portion of the existing European buildings stock. Their obsolescence in terms of design criteria, materials, and functionality is becoming a critical issue for guaranteeing adequate compliance with current structural codes. Recently, a new jacketing system based on the use of high-performance fiber-reinforced concrete (HPFRC) has been introduced for strengthening existing RC building members. Despite the promising aspects of the HPFRC jacketing technique, currently, a comprehensive and systematic technical framework for its implementation is still missing. In this paper, the experimental performance of RC columns strengthened with the HPFRC jacket subjected to pure axial load and combined axial load-bending moment uncoupled from shear is investigated. The test outcomes confirmed a significant improvement of the structural performance for the strengthened columns, especially for higher values of eccentricity. Finally, a standard-based practice-oriented analytical tool for designing retrofit interventions using the HPFRC jacket is proposed. The comparison between the calculated and experimental results revealed a satisfactory prediction capability

Managing benign tracheal stenosis during COVID-19 outbreak

If elective surgery has been recommended to be postponed some diseases could potentially become life-threatening and cannot be delayed. Among these conditions, tracheal idiopathic stenosis, primary caused by endotracheal intubation or tracheostomy, usually become symptomatic when reach 50% obstruction. Endoscopic procedures could be considered as frst treatment in selected patients after stenosis evaluation, such as non-complex stenosis with low grade of cartilage involvement or tracheomalaci

Employing Asset Management to Control Costs and Sustain Highway Levels of Service: Volumes I and II

This project investigated the impact of varying two elements of pavement, bridge, and mobility asset management on the long term network-level performance of those assets. The first element was the condition at which restorative treatments are triggered. The second element was the budget available to implement these treatments. For each of the pavement, bridge and mobility assets, three different management strategies in the form of treatment trigger values were investigated: a standard treatment trigger strategy, an early treatment trigger strategy, and a late treatment trigger strategy. For the pavement and bridge assets, the standard treatment trigger strategy simulates INDOT’s current treatment trigger policy. The early treatment trigger strategy simulates performing treatment at a better condition level than the standard treatment trigger strategy, while the late treatment trigger strategy simulates deferring treatment to a worse condition level than the standard treatment trigger strategy. For bridge assets, each treatment trigger strategy had six different treatments that can be applied to different bridge components. It was discovered that the standard (current) treatment trigger strategy outperforms other strategies. For pavement assets, the treatment trigger strategies pertained to pavement rehabilitation treatments. It was found that the long-term pavement roughness condition at the network-level is highly influenced by the policy being used to trigger pavement rehabilitation. The higher the trigger standard, the higher the percentage of miles in good condition and the lower the percentage of miles in poor condition. For mobility management, the treatment trigger strategies pertained to lane additions. It was discovered that neither restricting nor expanding the lane addition project candidate list (corresponding to the late and early treatment trigger strategies, respectively) provides more cost efficiency in the long-term for reducing the percent of road miles or VMT experiencing peak hour congestion. The report consists of two volumes; the first volume reports the analysis results for the bridge asset class and the second volume reports the analysis results for the pavement and mobility asset classes

H-BIM – Innovative and Digital Tools to Improve the Management of the Existing Buildings

With the introduction of the BIM methodology, the digitalization is consolidating mostly the process of new building design defining new standardized working procedures in order to optimize the data flow. On the contrary, pertaining to existing buildings, the rules are still dictated by the traditional methodology leading to fragmented and disaggregated information flow. However, some tools have been recently developed and are quickly upgrading to meet the specific aim to pursue progress, dynamism and experimentation in order to stay in tune with market demands. One common issue, is due to the fact that the documents are paper, hard to find and numerous so that the digitalization represents the best real solution to improve their management. In this regard, the heart of the informative flow is represented by the Common Data Environment (CDE), a cloud storage in which the documents are collected once uploaded and easily manageable with specific platforms. By way of example, a case study is developed in the paper in which the management process of the H-BIM of the XV century’s structure placed in the old town of Naples is carried out in a digital way, through the usBIM.platform (released by ACCA software company) using specific tools, like links, markers and tags. The goal of the paper is to define both a new organization for the data archive, in which the documents are structured and easily traceable, both a new way of conceiving the BIM model that it is thought as an informative vehicle and a key access to the information rather than a mere geometric representation

Knowledge and valorization of historical sites through 3D documentation and modeling

The paper presents the first results of an interdisciplinary project related to the 3D documentation, dissemination, valorization and digital access of archeological sites. Beside the mere 3D documentation aim, the project has two goals: (i) to easily explore and share via web references and results of the interdisciplinary work, including the interpretative process and the final reconstruction of the remains; (ii) to promote and valorize archaeological areas using reality-based 3D data and Virtual Reality devices. This method has been verified on the ruins of the archeological site of Pausilypon, a maritime villa of Roman period (Naples, Italy). Using Unity3D, the virtual tour of the heritage site was integrated and enriched with the surveyed 3D data, text documents, CAAD reconstruction hypotheses, drawings, photos, etc. In this way, starting from the actual appearance of the ruins (panoramic images), passing through the 3D digital surveying models and several other historical information, the user is able to access virtual contents and reconstructed scenarios, all in a single virtual, interactive and immersive environment. These contents and scenarios allow to derive documentation and geometrical information, understand the site, perform analyses, see interpretative processes, communicate historical information and valorize the heritage location

Geometric calibration and radiometric correction of the maia multispectral camera

Multispectral imaging is a widely used remote sensing technique, whose applications range from agriculture to environmental monitoring, from food quality check to cultural heritage diagnostic. A variety of multispectral imaging sensors are available on the market, many of them designed to be mounted on different platform, especially small drones. This work focuses on the geometric and radiometric characterization of a brand-new, lightweight, low-cost multispectral camera, called MAIA. The MAIA camera is equipped with nine sensors, allowing for the acquisition of images in the visible and near infrared parts of the electromagnetic spectrum. Two versions are available, characterised by different set of band-pass filters, inspired by the sensors mounted on the WorlView-2 and Sentinel2 satellites, respectively. The camera details and the developed procedures for the geometric calibrations and radiometric correction are presented in the paper

GEOMETRIC CALIBRATION AND RADIOMETRIC CORRECTION OF THE MAIA MULTISPECTRAL CAMERA

Multispectral imaging is a widely used remote sensing technique, whose applications range from agriculture to environmental monitoring, from food quality check to cultural heritage diagnostic. A variety of multispectral imaging sensors are available on the market, many of them designed to be mounted on different platform, especially small drones. This work focuses on the geometric and radiometric characterization of a brand-new, lightweight, low-cost multispectral camera, called MAIA. The MAIA camera is equipped with nine sensors, allowing for the acquisition of images in the visible and near infrared parts of the electromagnetic spectrum. Two versions are available, characterised by different set of band-pass filters, inspired by the sensors mounted on the WorlView-2 and Sentinel2 satellites, respectively. The camera details and the developed procedures for the geometric calibrations and radiometric correction are presented in the paper