INNOVATION TECHNOLOGY IN FEED FORMULATION AND PRODUCTION

The general aim of this thesis was to investigate new technology in feed formulation and production. For this purpose several aspects in the field of feed production were investigated. Specifically, during the course of this PhD program, three main sub aims have been addressed: i) evaluation of new ingredients, i.e., insect materials, in feed formulation and the impact of feed technology on their nutritional value; ii) improvements in the official methods for detecting Processed Animal Proteins (PAPs), and iii) investigation of the innovation, research, and development needs in in the feed industry in two selected areas. With respect to the first sub aim, fresh insect (Hermetia illucens) material was evaluated as an alternative protein source in experimental feed. Differents experimental formulae containing insect material were tested under differing extrusion conditions. Extruded feed was also evaluated for digestibility. The results indicate that fresh Hermetia illucens material can be efficiently included in experimental extruded feed containing 25% insect material and 75% wheat. Technological treatment, i.e., extrusion, increased in vitro organic matter digestibility, and did not affect protein digestibility. With regard to the second sub-aim the basic assumption was that insect material, if authorized, should be considered as animal material. Accordingly, the second sub aim of the thesis focused on implementing existing methods for processing animal protein and investigating their potential in tracing and characterizing insect material. In this scenario, experiments were aimed toward improving the official microscopy techniques for detecting processed animal proteins (PAP) by combining those with image analysis (IA) technology. The studies conducted aim to i) characterize fish meal material in compound feed (i.e., aquafeed), ii) identify specific selected markers able to efficiently distinguish between fish and terrestrial materials, iii) distinguish between mammalian materials, and iv) verify the applicability of the method for identifying insect material in feed. The results obtained in this context indicated that even though microscopy seems to be a promising approach for identifying both animal proteins and insect material, using microscopy alone has some limitations; therefore, a combined approach with other methods (i.e., PCR) is recommended. With regard to the third sub aim, research and development needs and innovation in the feed industry, the results of a targeted survey conducted in two countries (Italy and Serbia) showed that innovation in raw materials is a key factor for large multinational industries. In contrast, the survey results obtained from small and medium feed companies are quite different; for these companies, cost reduction, decreased energy consumption, improved quality, improved market image, development of new markets and satisfying market demand are much more important. By combining the results obtained from the various studies described in this paper, it can be concluded that: i) Insects show great potential as a protein source in animal feeds. Specific selected feed technologies, such as extrusion, can be useful in making such feeds convenient and safe to use. ii) Assuming that insect material will be authorized for use in animal feeds, existing methods for processing animal proteins may represent an advantageous starting point. Further investigation and implementation of methods of analysis is still required. iii) Even though insect materials as animal nutrition can be considered as a \u201chot topic\u201d from a scientific point of view, not everyone in the feed sector seems to be aware of the issue. Addressing \u201cnew ingredients\u201d, co- and by- products remain the main categories in the feed sector mind consciousness

Changes in the bacterial community of soil from a neutral mine drainage channel

Mine drainage is an important environmental disturbance that affects the chemical and biological components in natural resources. However, little is known about the effects of neutral mine drainage on the soil bacteria community. Here, a high-throughput 16S rDNA pyrosequencing approach was used to evaluate differences in composition, structure, and diversity of bacteria communities in samples from a neutral drainage channel, and soil next to the channel, at the Sossego copper mine in Brazil. Advanced statistical analyses were used to explore the relationships between the biological and chemical data. The results showed that the neutral mine drainage caused changes in the composition and structure of the microbial community, but not in its diversity. The Deinococcus/Thermus phylum, especially the Meiothermus genus, was in large part responsible for the differences between the communities, and was positively associated with the presence of copper and other heavy metals in the environmental samples. Other important parameters that influenced the bacterial diversity and composition were the elements potassium, sodium, nickel, and zinc, as well as pH. The findings contribute to the understanding of bacterial diversity in soils impacted by neutral mine drainage, and demonstrate that heavy metals play an important role in shaping the microbial population in mine environments95CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP550240/2010-12012/05429-

Characterization of the core microbiota of the drainage and surrounding soil of a brazilian copper mine

The core microbiota of a neutral mine drainage and the surrounding high heavy metal content soil at a Brazilian copper mine were characterized by 16S rDNA pyrosequencing. The core microbiota of the drainage was dominated by the generalist genus Meiothermus. The soil samples contained a more heterogeneous bacterial community, with the presence of both generalist and specialist bacteria. Both environments supported mainly heterotrophic bacteria, including organisms resistant to heavy metals, although many of the bacterial groups identified remain poorly characterized. The results contribute to the understanding of bacterial communities in soils impacted by neutral mine drainage, for which information is scarce, and demonstrate that heavy metals can play an important role in shaping the microbial communities in mine environments384484489CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP550240/2010-12012/05429-

Adaptive Polynomial Harmonic Distortion Compensation in Current and Voltage Transformers Through Iteratively Updated QR Factorization

Measuring current and voltage harmonics has paramount importance for improving the power quality of distribution grids. However, the achieved accuracy strongly depends on the adopted instrument transformer (IT). This article proposes an adaptive technique that enables an effective compensation of both the filtering behavior and the harmonic distortion (HD) introduced by current and voltage transformers (VTs), namely the strongest nonlinear effect at low-order harmonics. The approach is based on a flexible, linear in the parameters polynomial modeling of HD in the frequency domain. Model complexity can be different from one harmonic to the other, and it is selected through an automatic iterative process to suit the nonlinear behavior at each specific harmonic order, while avoiding overfitting. In particular, the number of parameters is increased by progressively updating the QR factorization of the regressor matrix trough Householder reflections until a convergence condition is reached. Experimental tests performed on an inductive VT and current transformer (CT) highlight the effectiveness of the approach

Frequency-domain nonlinear modeling approaches for power systems components - A comparison

Harmonic simulations play a key role in studying and predicting the impact of nonlinear devices on the power quality level of distribution grids. A frequency-domain approach allows higher computational efficiency, which has key importance as long as complex networks have to be studied. However, this requires proper frequency-domain behavioral models able to represent the nonlinear voltage-current relationship characterizing these devices. The Frequency Transfer Matrix (FTM) method is one of the most widespread frequency domain modeling approaches for power system applications. However, others suitable techniques have been developed in the last years, in particular the X-parameters approach, which comes from radiofrequency and microwave applications, and the simplified Volterra models under quasi-sinusoidal conditions, that have been specifically tailored for power system devices. In this paper FTM, X-parameters and simplified Volterra approaches are compared in representing the nonlinear voltage-current relationship of a bridge rectifier feeding an ohmic-capacitive dc load. Results show that the X-parameters model reaches good accuracy, which is slightly better than that achieved by the FTM and simplified Volterra models, but with a considerably larger set of coefficients. Simplified Volterra models under quasi-sinusoidal conditions allows an effective trade-off between accuracy and complexity

Review: Insects and former foodstuffs for upgrading food waste biomasses/streams to feed ingredients for farm animals

The increasing global need to find alternative protein/energy sources has triggered research in the field of non-conventional feed ingredients, with insects and former foodstuffs being the most promising. Insects contain high level of protein and fat, whereas former foodstuffs contain high energy in the form of carbohydrates and fats; therefore, both should be considered as promising alternative feed ingredients for livestock production. In addition to the nutritional value, they also represent a way by which food waste biomasses/streams can be upgraded to valuable feed ingredients. This review outlines the main nutritional and safety issues of insects and former foodstuffs, and also considers the legal framework involved. The importance of the type of insect metamorphosis and tailored substrates that could lead to the production of a premium feed is also described. This is also the first time that a review discusses the nutritional quality of former foodstuffs. Energy and the main nutrient content of former foodstuff are compared with the composition of common cereals as the principal energy sources in animal feed. For both ingredients a critical review of the safety issues is provided. Based on the current data available, both insects and former foodstuffs have an excellent potential use as alternative feed ingredients for livestock production. When produced in line with the criteria set by major feed/food authorities, they are characterized by high quality and safety standards. This makes them comparable to other feed materials and ingredients currently available on the market, although their full nutritional, functional, safety and sustainability evaluation cannot be considered complete

Comparative Proteomics of Milk Fat Globule Membrane (MFGM) Proteome across Species and Lactation Stages and the Potentials of MFGM Fractions in Infant Formula Preparation

Milk is a lipid-in-water emulsion with a primary role in the nutrition of newborns. Milk fat globules (MFGs) are a mixture of proteins and lipids with nutraceutical properties related to the milk fat globule membrane (MFGM), which protects them, thus preventing their coalescence. Human and bovine MFGM proteomes have been extensively characterized in terms of their formation, maturation, and composition. Here, we review the most recent comparative proteomic analyses of MFGM proteome, above all from humans and bovines, but also from other species. The major MFGM proteins are found in all the MFGM proteomes of the different species, although there are variations in protein expression levels and molecular functions across species and lactation stages. Given the similarities between the human and bovine MFGM and the bioactive properties of MFGM components, several attempts have been made to supplement infant formulas (IFs), mainly with polar lipid fractions of bovine MFGM and to a lesser extent with protein fractions. The aim is thus to narrow the gap between human breast milk and cow-based IFs. Despite the few attempts made to date, supplementation with MFGM proteins seems promising as MFGM lipid supplementation. A deeper understanding of MFGM proteomes should lead to better results

A Low-Cost Approach to the Skin Effect Compensation in Cylindrical Shunts

In this paper the development of a new design solution for high-current shunt resistors is presented, which allows achieving very good accuracy while requiring a simple and low-cost manufacturing process. It is based on a solid cylinder having the voltage measurement circuit which runs through two holes drilled in the cylinder itself. Starting from the well-known expression of the current density in a cylindrical conductor, the frequency response of the shunt is obtained in closed form as a function of the geometric parameters. In turn, the positions of the voltage measurement terminals are chosen by optimizing the frequency response function over a specified range. A shunt prototype has been manufactured and its measurement performance has been evaluated. The experimental results confirm the validity of the approach and highlight the significant improvement with respect to the single-hole cylindrical shunt which has been recently proposed by the authors. The obtained measurement accuracy is noticeable when compared with the ease of manufacturing

Multivariate image analysis for the rapid detection of residues from packaging remnants in former foodstuff products (FFPs)–a feasibility study

From a circular economy perspective, feeding livestock with food leftovers or former foodstuff products (FFPs) could be an effective option aimed at exploiting food leftover resources and reducing food losses. FFPs are valuable energy sources, characterised by a beneficial starch/sugar content, and also fats. However, besides these nutritional aspects, safety is a key concern given that FFPs are generally derived from packaged food. Packaging materials, such as plastics and paper, are not accepted as a feed ingredient which means that residues should be rigorously avoided. A sensitive and objective detection method is thus essential for an accurate risk evaluation throughout the former food production chain. To this end, former food samples were collected in processing plants of two different European countries and subjected to multivariate analysis of red, green, and blue (RGB) microscopic images, in order to evaluate the possible application of this non-destructive technique for the rapid detection of residual particles from packaging materials. Multivariate Image Analysis (MIA) was performed on single images at the pixel level, which essentially consisted in an exploratory analysis of the image data by means of Principal Component Analysis, which highlighted the differences between packaging and foodstuff particles, based on their colour. The whole dataset of images was then analysed by means of a multivariate data dimensionality reduction method known as the colourgrams approach, which identified clusters of images sharing similar features and also highlighted outlier images due to the presence of packaging particles. The results obtained in this feasibility study demonstrated that MIA is a promising tool for a rapid automated method for detecting particles of packaging materials in FFPs

Potentials and Challenges of Former Food Products (Food Leftover) as Alternative Feed Ingredients

Former food products (FFPs) are foodstuffs that, even though they are nutritious and safe, have lost their value on the human consumption market for different reasons, such as production errors leading to broken or intermediate foodstuffs, surpluses caused by logistical challenges of daily delivery, or any other reason. The nutritional features of FFPs include carbohydrates, free sugars, and possibly also fats. FFPs tend to have been processed through various technological and heat treatments that impact the nutrients and the kinetics of digestion, as well as animal response and, particularly, gastro-intestinal health. This review integrates some of the most recently published works about the chemical composition, nutritional value, digestibility and glycaemic index of ex-foods. In addition, a view on the relationship between the use of FFPs and safety issues and their effects on pigs\u2019 intestinal microbiota are also given