The Human SLC25A33 and SLC25A36 Genes of Solute Carrier Family 25 Encode Two Mitochondrial Pyrimidine Nucleotide Transporters

The human genome encodes 53 members of the solute carrier family 25 (SLC25), also called the mitochondrial carrier family, many of which have been shown to transport inorganic anions, amino acids, carboxylates, nucleotides, and coenzymes across the inner mitochondrial membrane, thereby connecting cytosolic and matrix functions. Here two members of this family, SLC25A33 and SLC25A36, have been thoroughly characterized biochemically. These proteins were overexpressed in bacteria and reconstituted in phospholipid vesicles. Their transport properties and kinetic parameters demonstrate that SLC25A33 transports uracil, thymine, and cytosine (deoxy)nucleoside di- and triphosphates by an antiport mechanism and SLC25A36 cytosine and uracil (deoxy)nucleoside mono-, di-, and triphosphates by uniport and antiport. Both carriers also transported guanine but not adenine (deoxy)nucleotides. Transport catalyzed by both carriers was saturable and inhibited by mercurial compounds and other inhibitors of mitochondrial carriers to various degrees. In confirmation of their identity (i) SLC25A33 and SLC25A36 were found to be targeted to mitochondria and (ii) the phenotypes of Saccharomyces cerevisiae cells lacking RIM2, the gene encoding the well characterized yeast mitochondrial pyrimidine nucleotide carrier, were overcome by expressing SLC25A33 or SLC25A36 in these cells. The main physiological role of SLC25A33 and SLC25A36 is to import/export pyrimidine nucleotides into and from mitochondria, i.e. to accomplish transport steps essential for mitochondrial DNA and RNA synthesis and breakdown

New Relation-Theoretic Fixed Point Theorems in Fuzzy Metric Spaces with an Application to Fractional Differential Equations

In this paper, we introduce the notion of fuzzy R − ψ−contractive mappings and prove some relevant results on the existence and uniqueness of fixed points for this type of mappings in the setting of non-Archimedean fuzzy metric spaces. Several illustrative examples are also given to support our newly proven results. Furthermore, we apply our main results to prove the existence and uniqueness of a solution for Caputo fractional differential equations

Disease-gene discovery by integration of 3D gene expression and transcription factor binding affinities

Abstract Motivation: The computational evaluation of candidate genes for hereditary disorders is a non-trivial task. Several excellent methods for disease-gene prediction have been developed in the past 2 decades, exploiting widely differing data sources to infer disease-relevant functional relationships between candidate genes and disorders. We have shown recently that spatially mapped, i.e. 3D, gene expression data from the mouse brain can be successfully used to prioritize candidate genes for human Mendelian disorders of the central nervous system. Results: We improved our previous work 2-fold: (i) we demonstrate that condition-independent transcription factor binding affinities of the candidate genes' promoters are relevant for disease-gene prediction and can be integrated with our previous approach to significantly enhance its predictive power; and (ii) we define a novel similarity measure—termed Relative Intensity Overlap—for both 3D gene expression patterns and binding affinity profiles that better exploits their disease-relevant information content. Finally, we present novel disease-gene predictions for eight loci associated with different syndromes of unknown molecular basis that are characterized by mental retardation. Contact: [email protected] or [email protected] Supplementary information: Supplementary data are available at Bioinformatics online

Fragmentation studies of high energy ions using CR39 nuclear track detectors

We report on the measurements of the total charge changing fragmentation cross sections in high-energy nucleus-nucleus collisions using Fe, Si and Pb incident ions. Several stacks of CR39 nuclear track detectors with different target combinations were exposed at normal incidence to high energy accelerator beams to integrated densities of about 2000 ions/cm^2. The nuclear track detector foils were chemically etched, and ion tracks were measured using an automatic image analyzer system. The cross section determination is based on the charge identification of beam ions and their fragments and on the reconstruction of their path through the stacks.Comment: 5 pages, 4 EPS figures. Corrected Eq. 3 and Table 1. Presented at the 10th Inter. Symp. Radiat. Phys., Coimbra, Portugal, 17-22 Sept. 200

Prevalence and correlates for self-reported sleep problems among nursing students

Introdution. University students report significantly worse sleep quality than the general population. Sleep problems are related to increased health concerns, irritability, depression, fatigue, attention and concentration difficulties, along with poor academic performance. The aim of this paper is to conduct a survey based on a questionnaire that would characterize night time and daytime habits in nursing students to estimate the prevalence of chronic insomnia, sleep disturbance and their correlates. Methods. We conducted a cross-sectional survey among 364 nursing students of the University of L?Aquila, in Italy. Self-reported sleep data were derived from Sleep and Daytime Habits Questionnaire (SDHQ) that covered sleep and daytime habits and academic progress. Anxiety and depression symptoms were assessed by the Mental Health Invenctory-5 (MHI-5) questionnaire. A supplement includes information about lifestyle, health status and physical activity. Results. The overall prevalence of insomnia was 26,7%. It increased significantly from 10,3% for students aged inf. 20 years to 45,5% for those aged over 40 years. The prevalence of sleep problems were 9,4% for disorders of initiating sleep, 8,3% for disrupted sleep, 7,7% for early morning awakening and subjectively poor quality of sleep 22,3%. Multiple logistic regression analysis showed that greater age was significantly associated with an increased risk of insomnia. Other risk predictors of insomnia were headache, severe depression and self perception of poor quality of life. Daytime sleepiness and morning tiredness were significantly associated with current smoking habit and painful physical condition. The risk of unsatisfactory academic progress increased significantly in students reported poor sleep quality. Discussion. Our study demonstrates that sleep problems are very common among students, and supports the need to assess sleep prob-lems and identify students at risk regarding school achievement

Search for Intermediate Mass Magnetic Monopoles and Nuclearites with the SLIM experiment

SLIM is a large area experiment (440 m2) installed at the Chacaltaya cosmic ray laboratory since 2001, and about 100 m2 at Koksil, Himalaya, since 2003. It is devoted to the search for intermediate mass magnetic monopoles (107-1013 GeV/c2) and nuclearites in the cosmic radiation using stacks of CR39 and Makrofol nuclear track detectors. In four years of operation it will reach a sensitivity to a flux of about 10-15 cm-2 s-1 sr-1. We present the results of the calibration of CR39 and Makrofol and the analysis of a first sample of the exposed detector.Comment: Presented at the 22nd ICNTS, Barcelona 200

Citron Kinase Is a Cell Cycle-dependent, Nuclear Protein Required for G 2 /M Transition of Hepatocytes

Citron Kinase (Citron-K) is a cell cycle-dependent protein regulating the G(2)/M transition in hepatocytes. Synchronization studies demonstrated that expression of the Citron-K protein starts at the late S and/or the early G(2) phase after that of cyclin B1. Expression of Citron-K is developmentally regulated. Levels of Citron-K mRNA and protein are highest in embryonic liver and gradually decrease after birth. Citron-K exists in interphase nuclei and begins to disperse into the cytoplasm at prophase. It concentrates at the cleavage furrow and midbody during anaphase, telophase, and cytokinesis, implicating a role in the control of cytokinesis. However, studies with knockouts show that Citron-K is not essential for cytokinesis in hepatocytes. Instead, loss of Citron-K causes a significant increase of G(2) tetraploid nuclei in one-week-old rat and mouse liver. In addition, Citron-K deficiency triggers apoptosis in a small subset of embryonic liver cells. In summary, our data demonstrate that Citron-K has a distinct cell cycle-dependent expression pattern and cellular localization as a downstream target of Rho-GTPase and functions in the control of G(2)/M transition in the hepatocyte cell cycle

Stability in a long length NbTi CICC

A crucial issue for a superconducting coil in order to be safely used in the magnetic system of a fusion reactor is stability against all foreseen disturbances. To simulate the fusion machine conditions, including off-normal events, e.g. plasma disruptions, the energy deposition has to be spread over a "long length" cable in conduit conductor (CICC) and a background magnetic field is needed. We have therefore designed and built an experiment consisting of an instrumented NbTi test module inserted in a pair of co-axial pulsed copper coils. A 0.6 m diameter superconducting coil provides a background magnetic field up to 3 T. Calibration of the energy inductively coupled between the pulsed coils and the module has been obtained measuring the system temperature increase just after the pulse by means of thermometers positioned along the conductor. Stability vs. operating current I/sub op/ has been examined for different helium temperatures and different background magnetic fields. The finite element code Gandalf for the stability and quenching transients analysis in forced flow cooled superconducting coils has been run to check the matching with the experimental results. (3 refs)

Search for spontaneous muon emission from lead nuclei

We describe a possible search for muonic radioactivity from lead nuclei using the base elements ("bricks" composed by lead and nuclear emulsion sheets) of the long-baseline OPERA neutrino experiment. We present the results of a Monte Carlo simulation concerning the expected event topologies and estimates of the background events. Using few bricks, we could reach a good sensitivity level.Comment: 12 pages, 4 figure