Kemampuan Fertilisasi Spermatozoa Sexing dan Perkembangan Awal Embrio secara In Vitro pada Sapi

The aim of the present study was to investigate the fertilization ability of bovine oocytes and early bovine embryonic development in vitro, fertilized by frozen X and Y sperm separated by bovine serum albumin (BSA) gradient column. Oocytes were collected from slaughter house ovarian by flushing and slicing technique. Oocytes were than maturated in tissue culture medium (TCM) 199 supplemented with 10 IU/ml pregnant mare's serum gonadotropin (PMSG), 10 IU/ml human chorionic gonadotropin (hCG) and 10% fetal bovine serum (FBS) for 24 h in 5% CO2 incubator 39oC. Oocytes then fertilized with three kind of different frozen spermatozoa (X,Y and unsexing spermatozoa as control) for 14 h with final concentration 2x106 spermatozoa/mL. Embryos were cultured insynthetic oviductal fluid (SOF) supplemented with essential and non essential amino acid and 0.3% bovine serum albumin (BSA) for 96 h. Results of the experiments revealed that there was no significant difference (P>0.05) in thefertilization ability (49.17%; 51.40%; 53.42%) for X, Y and control group, respectively. No significant difference (P>0.05) in the number of embryos development (47.77%; 48.25%; 54.43%) for X, Y and control group, respectively. Furthermore, only small number of embryos could pass development blockade (23.80%; 26.08%; 23.61%) for X, Y and control spermatozoa with statistically no significant difference (P>0.05). It is concluded that sexed spermatozoa separated by BSA gradient column had comparable fertilization ability with unsexing spermatozoa and had ability to supported early embryonic development

Pengaruh Pengawetan terhadap Kekuatan dan Keawetan Produk Laminasi Bambu

The need of housing corresponds with the increasing population growth. Such condition affects the demand of timber used for building construction. On the other hand, the government extremly minimizes forest logging to protect the forest from degradation. To solve the problem of increasing need of timber, one of the efforts is by utilzing bamboo as local materials for laminated products. However besides the advantages, bamboo is a susceptible material for organism. Improving the durability of bamboo againts organism can be achieved through preservation.This research (study) is aimed to recognize the effect of bamboo preservation to the strength and durability of laminated bamboo. In this study, Petung bamboo (Dendrocalamus sp) was treated with boron plus 63% preservative with the concentration of 1.57% and 3.15% using Bouherie-Morisco method with 3 kg/cm2 and 4kg/cm2 air pressure. The glulam bamboos in 25 mm x 30 mm x 2000 mm dimension were made from preserved bamboo with polimer isocyanate adhesive in 50#/MSGL under 1.2 Mpa pressure. Untreated bamboo were used as control for each treatment, 3 aplications were applied. Results of this research showed that moisture content was affected by the preservation pressure. The preservation treatments were not affected by the density, compressive strength parallel to the grain, tensile strength parallel to the grain, internal bond strength, horizontal bending strength, vertical bending strength, horizontal modulus of elasticity, vertical modulus of elasticity of the tested glulam bamboo as well as retention of preservatives was affected by the concentration of preservatives or the pressure of the treatment. The treatment method affected the termites mortality percentage but not the mass lost percentage. The physical and mechanical properties of treated bamboo was not indefference to the untreated bamboo. There was a tendency that mass lost precentage in untreated bamboo was higher than in treated bamboo while the termite mortality percentage in treated bamboo was lower than un treated bamboo

Analysis on Patien's Linen in Management of Permata Bunda Hospital Purwodadi

Based on bed occupancy ratio (BOR) in 2010, it was known that the number of inpatient visit atPermata Bunda hospital was relatively high. As a consequence, the need of linen increased. Basedon preliminary study, it was known that planning, implementation, and controlling of linen werenot adequate. The objective of this study was to know how linen in Permata Bunda hospital Purwodadi was managed.This was a descriptive explorative study with cross sectional approach. Study population was workersof Permata Bunda hospital Purwodadi who were involved in linen management. Main informantswere chief of nurse in the general ward, chief and assistance chief of linen logistic unit, chief andassistant chief of laundry unit. Triangulation informants were general manager, logistic unit chiefand patients.Results of the study showed that planning for linen requirement estimation was not good because it wasdone based on lost or damaged linen assumptions. Ordering process was still insufficient due to nospecial staff who ordered the linen. Supply linen was inadequate due to no standard for linen supply.Maintenance of linen was good; it was performed according to the right phases and procedures.Distribution of linen was not good due to separation of the rooms by the street. Linen service to patientwas inadequate; not all patients' linen was changed once a day. Linen management organization wasnot good; it was still done together. Linen stock control was not good; calculation for linen requirementwas still lacking, and it had not used the standard of 3 times of the number of bed. Implementation oflinen inventory was still inadequate; no specific recording was done according to the dirtiness level ofthe linen. Linen coding or identification was still inadequate; coding was not in detail.Based on the results of the study, it is suggested that calculation of linen requirement should bedone based on the number of bed with availability of 3 par stock linen in each bed. Written standardoperating procedure in linen management should be produced; it will be used as guideline orstandard by linen staffs or management. Identification or coding of linen should be improved; itincludes hospital name, room name, type of linen and type of material

Evaluation of BIM Education for Quantity Surveying: A Review of Teaching Approaches

Building Information Modelling (BIM) technology has become increasingly well-known among construction industry players throughout the world. It is known as a process that offers numerous benefits by the implementation in the industry. Almost 50% of practitioners in construction industry is using BIM. Quantity Surveyors (QS) are one of the important main professionals in construction industry who should have an adequate and sufficient BIM knowledge and skills. Equivalent to this, based on the educational perspective it is clear that there is a growing need for universities to provide their graduates with appropriate BIM-related skills. Although the educational frameworks that have been established for academic purposes in Malaysia, still, it is in doubt whether this framework is parallel with industry’s demand and also whether the knowledge and skills provided sufficient with industry’s requirements. It is crucial to recognize the applicable teaching approach for BIM educations in order to ensure students capable in applying BIM tools and meet the expectation of industry. This paper reviews the BIM teaching approaches and the BIM module applied in QS undergraduate program.   Keywords: BIM, quantity surveyors, QS education, teaching approach, Malaysi

PEMBUATAN BAHAN AJAR DAN MEDIA ONLINE BERBASIS KURIKULUM 2013 OLEH GURU-GURU SD SE-KECAMATAN PEDURUNGAN

ABSTRAKSaat ini pembelajaran di Sekolah Dasar masih terpaku pada pembelajaran di dalam kelas. Walaupun beberapa sekolah sudah menggunakan media dalam pembelajaran, namun dengan adanya internet yang memadai, guru dapat meningkatkan penggunaan media pembelajaran tersebut secara online. Berdasarkan hal tersebut maka dibuatlah pelatihan pembuatan media pembelajaran online yang memungkinkan guru dan siswa melakukan pembelajaran jarak jauh dengan menggunakan bantuan internet. Diharapkan dengan dipergunakannya bahan ajar dan media online, pembelajaran akan berlangsung lebih fleksibel. Tujuan kegiatan ini adalah untuk meningkatkan kemampuan guru untuk menyediakan bahan ajar dan media online sehingga pembelajaran tidak hanya terpaku pada ruang kelas, tetapi dapat dengan mudah diakses oleh siswa. Kegiatan ini dilaksanakan pada tanggal 10-12 Januari 2015 di SD Negeri Pedurungan Tengah 01 dan diikuti oleh 7 sekolah dari Dabin Telaga Ilmu. Pada kegiatan ini peserta belajar cara membuat email dan blog, membuat bahan ajar online dengan menggunakan software FlipBook Maker, dan membuat tes online

Efektivitas Perlindungan Masker Gel Peel Off Ekstrak Etanol 96% Kulit Buah Manggis (Garcinia Mangostana L.) dalam Mempertahankan Ph Kulit Tikus Wistar Jantan yang Diberi Paparan Sinar Uv-b

Radiasi sinar UV-B memiliki kapasitas untuk menghasilkan reactive oxygen species (ROS) yang menyebabkan terjadinya photoaging yang dapat diamati dari nilai pH kulit. Ekstrak kulit buah manggis diketahui memiliki kandungan antioksidan yang tinggi dan telah diformulasi menjadi sediaan masker gel peel off . Sediaan ini diketahui mampu meningkatkan toleransi kulit tikus terhadap paparan sinar UV-B. Pada penelitian ini dilakukan pengujian untuk mengetahui efektivitas masker gel peel off ekstrak kulit buah manggis dalam menjaga pH kulit tikus wistar jantan yang diberi paparan sinar UV-B. Penelitian dilakukan dengan menggunakan 30 hewan uji yang dibagi dalam 3 kelompok, yaitu perlakuan I (basis masker gel peel off), perlakuan II (masker gel peel off ekstrak etanol 96% kulit buah manggis) dan kontrol (tanpa perlakuan). Kelompok perlakuan I dan perlakuan II dipapar dengan UV-B dengan dosis total 840 mJ/cm² selama 28 hari. Hasil penelitian menunjukkan bahwa kelompok perlakuan II berbeda bermakna dengan kelompok perlakuan I dan kelompok kontrol dengan nilai signifikansi P<0,05. Sehingga diketahui masker gel peel off ekstrak kulit buah manggis mampu mempertahankan pH kulit tikus wistar jantan yang dipapar sinar UV-B

Pengaruh Konsentrasi Kultur dan Prebiotik Ubi Jalar terhadap Sifat Sari Jagung Manis Probiotik

Sweet corn extract with the addition of lactic acid bacteria can improve its function as probiotic drinks. Sweet potato as a prebiotic is expected to increase the activity of lactic acid bacteria probiotic sweet corn extract. The use of culture in the making of sweet corn extract probiotics will affect the character of the product. The purpose of this study were: (1) to study the effect of culture concentration on characteristics of probiotic sweet corn extract; (2) to study the effect of red sweet potato extract on characteristics of probiotic sweet corn extract; and (3) to determine the best treatment combination between the culture and the concentration of red sweet potato extract in the production of probiotic sweet corn extract; and comparing commercial in the market. Research using a completely randomized design (CRD) with research factor is the concentration of the culture (2, 3, 4, and 5 %) and red sweet potato extract (5, 10, 15, 20 and 25 %). The results showed that the more the concentration of the culture is added, the number of lactic acid bacteria, total acid and viscosity increase; while pH, total dissolved solids, fat and protein concentration decreased. The more sweet potato extract is added, the total dissolved solids and viscosity increased; while the levels of protein and fat decreases. The best treatment combination was at a concentration of 4 % culture and 15 % sweet potato extract. The products have a pH of 3.88, a viscosity of 261.5 cP, lactic acid levels of 0.87 %, 0.05 % fat content, total dissolved solids 19.10 oBrix, and 3.23 % of total protein. Yoghurt is compliant SNI standard yoghurt, except for protein content was lower at 3.23 (SNI according to at least 3.5)

Pelatihan Dan Pendampingan Manajemen Peternakan Itik Dalam Rangka Meningkatkan Pendapatan Peternak Dan Populasi Plasma Nutfah Ternak Lokal Di Kelompok Ternak Itik Kebersamaan (K-tik) Dusun Ciherang Kidul (Bogor)

Duck farming business prospects are very promising especially in Bogor and surrounding areas . It is because the demand for meat and duck eggs are quite large and and business development is still slightly in the area of Bogor. Kelompok Ternak Itik Keberlanjutan ( KTIK ) is one of the groups of cattle that can catch these opportunities, however, due to limited knowledge of farm management and support facilities make this difficult group to fulfill market need. The PKM program using case study methods, training, collaboration, mentoring and evaluation for 5 months. Usefulness of the program to create new job opportunities and improving the lives of farmers, and stimulate students to think of creative, innovative, and dynamic. Results obtained from the PKM program is increasing basic knowledge about management duck farming which characterized by decreasing the percentage of answers does not know and incresing answer know and very aware. Income of the group increased from Rp Rp 775.000/month to 2.857.600/month result of the average sales strategies. This will assist in the smooth and KTIK progress in the future in an effort of the duck. This program is in collaboration with Himpunan Mahasiswa Nutrisi dan Makanan Ternak (HIMASITER ) to conduct training and supervision during the agreed cooperation agreement

Ultra-Morphological Changes of Trichophyton Rubrum Treated with Hydroxychavicol

Trichophyton rubrum is a common pathogenic fungal species that is responsible for causing infection on human skin, hair and nail. The antifungal-resistant strains complicate the treatment regime. Hydroxychavicol (HC) is one of the main compounds from Piper betel leaf that have antifungal potential and its mechanism of action has not been studied yet. The objective of this preliminary study to determine the antifungal properties of HC against T. rubrum using transmission electron microscope (TEM) on gross and ultrastructure of T. rubrum hypha. T. rubrum was treated with HC and miconazole (MI) at concentrations of 1.25, 2.5, 5 and 10 mg/mL for 1, 3, 5 and 7 days continuously. Generally, fungi structures became more severely damaged at increasing treatment duration. Microscopically, the fungi’s cell wall treated with HC showed a rough surface, shrinkage and demolition similar to the MI treated group. The fungi organelles were also demolished and disorganized. This study revealed that HC has the ability to inhibit T. rubrum growth and has potential to be an antifungal agent for skin infections