Biological welfare and nutritional inequality in rural mediterranean Spain: the irrigated area of Valencia, 1859-1939

This article analyses the biological welfare and inequality of the male population of the irrigated area of Valencia between 1859 and 1939. It studies the effects that the agrarian development process had on physical welfare and the relationship between height and access to land ownership. Height data for conscripts in five municipalities constitute the source for the study. The results reveal that there was a growing trend in the evolution of heights in the irrigated area of Valencia at the beginning of agrarian capitalism. Nutritional inequalities can be observed between farmers and farm workers: land owners were taller than landless labourers. However, this biological inequality diminished over the period under study

Application of low-cost sensors for the development of a methodology to design front-end loaders for tractors

Tractor front-end loaders are an essential part of the equipment used on farms. At present, there are an important number of small- and medium-sized companies involved in the manufacturing of this equipment. These companies rely heavily on experience for innovative designs, as in the vast majority of cases they lack access to adequate methodology for the optimal design of new front-end loaders. The study conducted has developed a methodology to design tractor front-end loaders with a view of obtaining their accurate design during the bucket loading process. The methodology comprises two phases: the first phase involves a numerical analysis of the structural behaviour of the front-end loader components by means of the Finite Element Method; the second phase, the experimental phase, makes use of low-cost sensors, in particular, strain gauges, to analyse existing strains at selected points in the front-end loader structure. The experimental results obtained by means of low-cost sensors fitted onto the front-end loader allow analysing the existing strains at the points measured, as well as validate the numerical model developed. This methodology is validated by applying it to a commercial front-end loader, more specifically to model 430E2 of the company Maquinaria Agrícola El León S.A (Spain)

PENGARUH PEMBERIAN PROBIOTIK DENGAN DOSIS YANG BERBEDA TERHADAP PERTUMBUHAN BENIH IKAN BANDENG (Chanos chanos FORKSAL)

Milkfish is one commodity that has high economic value because it is important in the fulfillment of community nutrition. This study aims to determine the provision of probiotics from banana stumps, with different doses in artificial feed on the growth of milkfish seed (Chanos chanos Forskal). The study used a completely randomized design with 4 treatments and 3 replications. The treatments were 3 cc / kg of feed, 6 cc / kg of feed, 9 cc / kg of feed and 12 cc / kg of feed. The research was conducted from 23rd May – 21st June 2015 at the Brackish Water Laboratory of Slamaran. The results showed that the highest average biomass growth in the treatment of doses of 12 cc / kg of feed reached 14.75 grams. The lowest yield was obtained in the treatment of 3 cc / kg of feed about 10.00 grams. The growth response to the proportional dose rate showed a linear form and the survival rate of larvae reached 100%. The water quality range during research is still appropriate to support growth, where temperatures range from 23 to 31 C. pH ranges from 6.5 to 7.5, dissolved oxygen content ranges from 5.5 to 5.6 ppm and salinity of 8-12 ppt.Key Word : Probiotics, growth, Milkfis

Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans

[EN]The aim of this work was to evaluate the biological activity of four grape pomace (GP) extracts that are rich in polyphenols using C. elegans as an in vivo model. Different concentrations of the GP extracts were assessed for their effects on the resistance of C. elegans against thermally induced oxidative stress, accumulation of reactive oxygen species (ROS), and lifespan. The cultivation of C. elegans with relatively low concentrations of GP extracts increased their resistance against thermal stress and prolonged their lifespan, while high levels displayed detrimental effects. In the studied extracts, maximum protection was observed for levels of polyphenols around 7 to 9 µg gallic acid equivalents per cultivation plate. The obtained results suggested that small changes in the ROS levels could have beneficial effects, although further studies are required to fully understand the impact of the extracts and assayed doses on ROS levels to explain the mechanism that is involved in the observed effects

Differentiation status of primary chronic myeloid leukemia cells affects sensitivity to BCR-ABL1 inhibitors

Tyrosine kinase inhibitors (TKI) are the mainstay treatment of BCR-ABL1-positive leukemia and virtually all patients with chronic myeloid leukemia in chronic phase (CP CML) respond to TKI therapy. However, there is limited information on the cellular mechanisms of response and particularly on the effect of cell differentiation state to TKI sensitivity in vivo and ex vivo/in vitro. We used multiple, independent high-throughput drug sensitivity and resistance testing platforms that collectively evaluated 295 oncology compounds to characterize ex vivo drug response profiles of primary cells freshly collected from newly-diagnosed patients with BCR-ABL1positive leukemia (n = 40) and healthy controls (n = 12). In contrast to the highly TKI-sensitive cells from blast phase CML and Philadelphia chromosome-positive acute lymphoblastic leukemia, primary CP CML cells were insensitive to TKI therapy ex vivo. Despite maintaining potent BCR-ABL1 inhibitory activity, ex vivo viability of cells was unaffected by TKIs. These findings were validated in two independent patient cohorts and analysis platforms. All CP CML patients under study responded to TKI therapy in vivo. When CP CML cells were sorted based on CD34 expression, the CD34-positive progenitor cells showed good sensitivity to TKIs, whereas the more mature CD34-negative cells were markedly less sensitive. Thus in CP CML, TKIs predominantly target the progenitor cell population while the differentiated leukemic cells (mostly cells from granulocytic series) are insensitive to BCR-ABL1 inhibition. These findings have implications for drug discovery in CP CML and indicate a fundamental biological difference between CP CML and advanced forms of BCR-ABL1-positive leukemia.Peer reviewe

Establishing LA VIDA: A Community-Based Partnership to Prevent Intimate Violence against Latina Women

LA VIDA—the Southwest Detroit Partnership to Prevent Intimate Violence Against Latina Women— evolved in response to community concern about the problem of intimate partner violence (IPV) and the lack of culturally competent preventive and support services for Latino women and men in southwest Detroit. Since 1997, diverse organizations have mobilized as a community-academic partnership to ensure the availability, accessibility, and utilization of IPV services. This article describes and analyzes the evolution of LA VIDA within a community-based participatory research framework using a case study approach that draws on multiple data sources including group and individual interviews and field notes. The challenges and lessons learned in addressing a complex multifaceted problem such as IPV in an ethnic minority community are highlighted in an examination of the process of mobilizing diverse organizations, conducting community diagnosis and needs assessment activities, establishing goals and objectives within a social ecological framework, and integrating evaluation during the development phase.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66991/2/10.1177_109019819902600606.pd

On the causes of economic growth in Europe: why did agricultural labour productivity not converge between 1950 and 2005?

The objective of this study is to make a further contribution to the debate on the causes of economic growth in the European Continent. It explains why agricultural labour productivity differences did not converge between 1950 and 2005 in Europe. We propose an econometric model, one combining both proximate and fundamental causes of economic growth. The results show that the continuous exit of labour power from the sector, coupled with the increased use of productive factors originating in other sectors of the economy, caused the efficiency of agricultural workers to rise. However, we offer a complete explanation of the role played by institutions and geographical factors. Thus, we detect a direct and inverse relation between membership of the EU and the Communist bloc and the productivity of agricultural labour. In addition, strong support for agriculture affected productivity negatively

Exploring target genes involved in the effect of quercetin on the response to oxidative stress in Caenorhabditis elegans

This article belongs to the Special Issue Antioxidants Properties of Natural Products: A Themed Issue in Honor of Professor Isabel C.F.R. Ferreira.Quercetin is one the most abundant flavonoids in the human diet. Although it is well known that quercetin exhibits a range of biological activities, the mechanisms behind these activities remain unresolved. The aim of this work is to progress in the knowledge of the molecular mechanisms involved in the biological effects of quercetin using Caenorhabditis elegans as a model organism. With this aim, the nematode has been used to explore the ability of this flavonoid to modulate the insulin/insulin-like growth factor 1(IGF-1) signaling pathway (IIS) and the expression of some genes related to stress response. Different methodological approaches have been used, i.e., assays in knockout mutant worms, gene expression assessment by RT-qPCR, and C. elegans transgenic strains expressing green fluorescent protein (GFP) reporters. The results showed that the improvement of the oxidative stress resistance of C. elegans induced by quercetin could be explained, at least in part, by the modulation of the insulin signaling pathway, involving genes age-1, akt-1, akt-2, daf-18, sgk-1, daf-2, and skn-1. However, this effect could be independent of the transcription factors DAF-16 and HSF-1 that regulate this pathway. Moreover, quercetin was also able to increase expression of hsp-16.2 in aged worms. This observation could be of particular interest to explain the effects of enhanced lifespan and greater resistance to stress induced by quercetin in C. elegans, since the expression of many heat shock proteins diminishes in aging worms.This research was funded by the Spanish Ministerio de Economía y Competitividad (MINECO Projects AGL2015-64522-C2 and BFU2015-64408-P) and Fondo Europeo de Desarrollo Regional (FEDER)-Interreg España-Portugal Programme (Project ref. 0377_IBERPHENOL_6_E)

screenwerk: a modular tool for the design and analysis of drug combination screens.

MOTIVATION: There is a rapidly growing interest in high-throughput drug combination screening to identify synergizing drug interactions for treatment of various maladies, such as cancer and infectious disease. This creates the need for pipelines that can be used to design such screens, perform quality control on the data and generate data files that can be analyzed by synergy-finding bioinformatics applications. RESULTS: screenwerk is an open-source, end-to-end modular tool available as an R-package for the design and analysis of drug combination screens. The tool allows for a customized build of pipelines through its modularity and provides a flexible approach to quality control and data analysis. screenwerk is adaptable to various experimental requirements with an emphasis on precision medicine. It can be coupled to other R packages, such as bayesynergy, to identify synergistic and antagonistic drug interactions in cell lines or patient samples. screenwerk is scalable and provides a complete solution for setting up drug sensitivity screens, read raw measurements and consolidate different datasets, perform various types of quality control and analyze, report and visualize the results of drug sensitivity screens. AVAILABILITY AND IMPLEMENTATION: The R-package and technical documentation is available at https://github.com/Enserink-lab/screenwerk; the R source code is publicly available at https://github.com/Enserink-lab/screenwerk under GNU General Public License v3.0; bayesynergy is accessible at https://github.com/ocbe-uio/bayesynergy. Selected modules are available through Galaxy, an open-source platform for FAIR data analysis at https://oncotools.elixir.no. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online