Transporte inter-hospitalar de crianças criticamente doentes = Interhospital transport of critically ill children

Objetivos: Realizar uma revisão atualizada sobre o transporte inter-hospitalar de crianças criticamente doentes Fonte de dados: Foi realizada uma pesquisa bibliográfica utilizando os banco de dados MEDLINE e LILACS. Foram citados apenas os artigos mais relevantes, além de livros textos Síntese dos dados: A criança criticamente doente, que necessita de transferência para unidade de tratamento terciário, se beneficia do atendimento por equipe especializada. É recomendado um período de estabilização previamente ao transporte, com especial atenção a via aérea e circulação. O modo de transporte deve ser adequado para acomodar o paciente e a equipe de forma segura, contendo materiais, equipamentos e medicamentos necessários para um bom atendimento Conclusões: O transporte inter-hospitalar de crianças graves pode ser realizado de forma rápida e segura por equipe treinada, com redução de morbimortalidad

Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules

Para se assegurar que um método analítico produzirá informação confiável e interpretável sobre a amostra este deve ser inicialmente validado, tornando claro que os resultados podem ser confiados e rastreados. Neste estudo, propomos validar um método de cromatografia líquida de alta eficiência (CLAE) para a quantificação do meloxicam encapsulado em nanocápsulas PEGuiladas (M-PEGNC). Efetuamos a validação, avaliando parâmetros de especificidade, linearidade, limite de quantificação, limite de detecção, exatidão, precisão e robustez. As nanocápsulas PEGuiladas foram preparadas por deposição interfacial do polímero pré-formado e caracterizaram-se o tamanho da partícula, índice de polidispersão, potencial zeta, pH e eficiência de encapsulação. O método de CLAE proposto fornece resultados seletivos e lineares na faixa de 1,0-40,0 mg/mL; limites de quantificação e detecção de 1,78 mg/mL e 0,59 mg/mL, respectivamente; desvio padrão relativo para a repetibilidade de 1,35% e precisão intermediária de 0,41% e 0,61% para o analista 1 e analista 2, respectivamente; exatidão entre 99,23 e 101,79%; robustez entre 97,13 e 98,45% para a quantificação de M-PEGNC. Os diâmetros médios das partículas foram 261 ± 13 nm e 249 ± 20 nm; índice de polidispersão de 0,15 ± 0,07 e 0,17 ± 0,06, valores de pH de 5,0 ± 0,2 e 5,2 ± 0,1 e valores do potencial zeta de -37,9 ± 3,2 mV e -31,8 ± 2,8 mV para o M-PEGNC e o placebo(B-PEGNC), respectivamente. Concluindo, o método analítico proposto é adequado para o controle de qualidade do M-PEGNC. Além disso, suspensões mostraram distribuição de tamanho monomodal e baixo índice de polidispersão, indicando alta homogeneidade das formulações com distribuição estreita de tamanho, pH e potencial zeta apropriados. O processo de extração foi eficiente para a liberação do meloxicam dos sistemas nanoestruturados.A method to ensure that an analytical method will produce reliable and interpretable information about the sample must first be validated, making sure that the results can be trusted and traced. In this study, we propose to validate an analytical high performance liquid chromatography (HPLC) method for the quantitation of meloxicam loaded PEGylated nanocapsules(M-PEGNC). We performed a validation study, evaluated parameters including specificity, linearity, quantification limit, detection limit, accuracy, precision and robustness. PEGylated nanocapsules were prepared by interfacial deposition of preformed polymer, and the particle size, polydispersity index, zeta potential, pH value and encapsulation efficiency were characterized. The proposed HPLC method provides selective, linear results in the range of 1.0-40.0 μg/mL; quantification and detection limits were 1.78 μg/mL and 0.59 μg/mL, respectively; relative standard deviation for repeatability was 1.35% and intermediate precision was 0.41% and 0.61% for analyst 1 and analyst 2, respectively; accuracy between 99.23 and 101.79%; robustness between 97.13 and 98.45% for the quantification of M-PEGNC. Mean particle diameters were 261 ± 13 nm and 249 ± 20 nm, polydispersity index was 0.15 ± 0.07 and 0.17 ± 0.06, pH values were 5.0 ± 0.2 and 5.2 ± 0.1, and zeta-potential values were -37.9 ± 3.2 mV e -31.8 ± 2.8 mV for M-PEGNC and placebo(B-PEGNC), respectively. In conclusion, the proposed analytical method is suitable for the quality control of M-PEGNC. Moreover, suspensions showed monomodal size distributions and low polydispersity index indicating high homogeneity of formulations with narrow size distributions, and appropriate pH and zeta potential. The extraction process was efficient for release of meloxicam from nanostructured systems

Mineração de dados na predição do tempo de imobilidade de camundongos no teste de nado forçado.

Este trabalho tem como objetivo colher informações sobre camundongos, expostos a um fármaco na sua forma livre e nanoencapsulado avaliados no teste do nado forçado. Os resultados foram aplicados em um algoritmo de mineração de dados que pode identificar padrões que permitem a predição do tempo, por grupo de avaliação, com acertividade de 78,19 %

Safety profile and prevention of cognitive deficit in alzheimer’s disease model of graphene family nanomaterials, Tucuma oil (Astrocaryum Vulgare) and its synergisms

Alzheimer\u27s disease is a worldwide health issue, and there are currently no treatments that can stop this disease. Oxidized graphene derivatives have gained prominence in use in biological systems due to their excellent physical-chemical characteristics, biocompatibility and ability to overcome the blood-brain barrier. Other substances highlighted are those of natural origin from the Amazon biome, such as tucuma, a fruit whose oil has been widely studied in therapeutic applications. Thus, the aim of this study was to investigate the action of graphene oxide, reduced graphene oxide and tucuma oil, isolated and combined, as an alternative for treatment of Alzheimer\u27s disease through studies in silico, in vitro, in vivo and ex vivo. Computational simulation via docking was used to verify the affinity of the substances with the proteins β-amyloid and acetylcholinesterase, in which the reduced graphene oxide was the one that showed the most favorable interaction. The results of the ab initio simulation showed that the synergism between the nanostructures and the oil occurs through physical adsorption. The experimental results revealed that the substances and their combinations were nontoxic, both at the cellular and systemic level. In general, all treatments had positive results against induced memory deficit, but reduced graphene oxide was the most prominent, as it was able to protect against memory damage in all behavioral tests performed, with anticholinesterase activity and antioxidant effect. In conclusion, the reduced graphene oxide is, among the treatments studied, the one with great therapeutic potential to be investigated in the treatment of this disease

INFLUENCE OF PEPTIDE P34 ON GENE EXPRESSION OF LISTERIA MONOCYTOGENES AND LISTERIA SEELEGERI

Objective: Investigate the influence of the antimicrobial peptides P34 and nisin on the expression of genes associated with components of the cell surface of Listeria monocytogenes and Listeria seeligeri.Methods: Antimicrobial activity was determined by addition of peptide P34 and nisin (12.5 µg/ml) onto Brain Heart Infusion agar (BHI) plates previously inoculated with indicator strains (L. monocytogenes ATCC 7644 or L. seeligeri AC 82/4) after incubation for 24 h at 37 °C or 240 h at 4 °C. Ribonucleic acid (RNA) was directly extracted from bacterial colonies at the border of the inhibition zones, and the expression levels of genes D-alanine-D-alanyl carrier protein ligase (dltA), putative phospholipid lysinylation (Imo 1695) and EIIABMan of mannose-specific PTS (mptA) were determined using real-time PCR.Results: A non-significant increase in the levels of transcription of genes dltA, Imo1695 and mptA was observed for L. monocytogenes treated with peptide P34 or nisin. Both peptides caused a similar decrease in dltA gene expression in L. seeligeri. The expression of gene Imo1695 significantly decreased (about 2000-fold) after treatment with the peptide P34 at 37 °C, while at 4 °C a reduction of 12-fold and 5-fold was detected for P34 and nisin, respectively. A significant decrease in mptA gene expression was observed by exposition to peptide P34 (31.872-fold) and nisin (16.047-fold) for 24 h at 37 °C.Conclusion: The results suggest that both peptide P34 and nisin influence the expression of genes related with the cell-surface/cell-membrane structure of L. seeligeri and in lesser extent L. monocytogenes

Transporte inter-hospitalar de crianças criticamente doentes

Aims: To conduct an actual revision of interhospital transport of critically ill children. Source of data: A bibliographical research was carried out using the MEDLINE and LILACS database. Only the more relevant articles were quoted, as well as textbooks. Summary of the findings: A critically ill child, who needs to be transferred to a paediatric intensive care unit, is benefited by a specialize team. It is recommended a stabilization period previous to the transportation, with special attention to airway and circulation. The circumstances of the transport need to be adequate as to provide security and safeness of patient as well as the medical team. It also requires equipments and medicines needed in a treatment. Conclusions: The interhospital transport of children critically ill can be done in a safe and rapid way by a well trained medical team, with reduction of morbidity and mortality.Objetivos: Realizar uma revisão atualizada sobre o transporte inter-hospitalar de crianças criticamente doentes. Fonte de dados: Foi realizada uma pesquisa bibliográfica utilizando os banco de dados MEDLINE e LILACS. Foram citados apenas os artigos mais relevantes, além de livros textos. Síntese dos dados: A criança criticamente doente, que necessita de transferência para unidade de tratamento terciário, se beneficia do atendimento por equipe especializada. É recomendado um período de estabilização previamente ao transporte, com especial atenção a via aérea e circulação. O modo de transporte deve ser adequado para acomodar o paciente e a equipe de forma segura, contendo materiais, equipamentos e medicamentos necessários para um bom atendimento. Conclusões: O transporte inter-hospitalar de crianças graves pode ser realizado de forma rápida e segura por equipe treinada, com redução de morbimortalidade

Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules

abstract A method to ensure that an analytical method will produce reliable and interpretable information about the sample must first be validated, making sure that the results can be trusted and traced. In this study, we propose to validate an analytical high performance liquid chromatography (HPLC) method for the quantitation of meloxicam loaded PEGylated nanocapsules(M-PEGNC). We performed a validation study, evaluated parameters including specificity, linearity, quantification limit, detection limit, accuracy, precision and robustness. PEGylated nanocapsules were prepared by interfacial deposition of preformed polymer, and the particle size, polydispersity index, zeta potential, pH value and encapsulation efficiency were characterized. The proposed HPLC method provides selective, linear results in the range of 1.0-40.0 μg/mL; quantification and detection limits were 1.78 μg/mL and 0.59 μg/mL, respectively; relative standard deviation for repeatability was 1.35% and intermediate precision was 0.41% and 0.61% for analyst 1 and analyst 2, respectively; accuracy between 99.23 and 101.79%; robustness between 97.13 and 98.45% for the quantification of M-PEGNC. Mean particle diameters were 261 ± 13 nm and 249 ± 20 nm, polydispersity index was 0.15 ± 0.07 and 0.17 ± 0.06, pH values were 5.0 ± 0.2 and 5.2 ± 0.1, and zeta-potential values were -37.9 ± 3.2 mV e -31.8 ± 2.8 mV for M-PEGNC and placebo(B-PEGNC), respectively. In conclusion, the proposed analytical method is suitable for the quality control of M-PEGNC. Moreover, suspensions showed monomodal size distributions and low polydispersity index indicating high homogeneity of formulations with narrow size distributions, and appropriate pH and zeta potential. The extraction process was efficient for release of meloxicam from nanostructured systems

Diphenyl ditelluride induces neurotoxicity and impairment of developmental behavioral in rat pups

The purpose of the present study was to investigate if acute exposure to diphenyl ditelluride (PhTe)2 causes impairment of developmental behavioral performance in rat pups. Rat pups received a single subcutaneous injection of (PhTe)2 (0.1 mg kg-1, 3 mL kg-1) or vehicle (3 mL kg-1) at 14th postnatal day. After exposure to (PhTe)2, the general parameters of neurotoxicity, behavioral tasks, cerebral myelin content, histological analysis and acetilcholinesterase (AChE) activity were performed during seven days. The appearance of classic signs of toxicity, behavioral alterations and the reduction in myelin content were dependent on the time after (PhTe)2 exposure to pups. Neuronal damage, reduction of myelin content, and the increase in AChE activity occurred mainly at 4th and 5th day after (PhTe)2 exposure, indicating that the critical period of neurotoxicity is coincident with the major behavioral alterations. In conclusion, exposure to (PhTe)2 induced neurotoxicity and impairment of developmental behavioral in rat pups

INFLUÊNCIA DO EXERCÍCIO SOBRE FÍSICO SOBRE O ESTRESSE OXIDATIVO NA DPOC: REVISÃO BIBLIOGRÁFICA

http://dx.doi.org/10.5902/223658344426Objetivo: verificar a influência do exercício físico sobre o estresse oxidativo em indivíduos portadores de DPOC. Metodologia: revisão bibliográfica, realizada no período de março a setembro de 2011 nas bases de dados PubMed, Lilacs, PEDro e Scielo, com artigos científicos originais publicados entre 2000 e 2011. Resultados: foram encontrados sete trabalhos que contemplaram o tema proposto, sendo cinco que avaliaram o estresse oxidativo após exercícios agudos e dois que realizaram treinamento físico. Conclusões: através deste estudo foi possível observar que a prática de exercícios agudos aumenta os níveis oxidativos na DPOC, porém com o treinamento físico os níveis tendem a diminuir