Factors influencing the radiocarbon dating of human skeletal remains from the Dnieper River system: Archaeological and stable isotope evidence of diet from the Epipaleolithic to Eneolithic periods

Recent research has identified the existence of a freshwater reservoir effect influencing the radiocarbon dating of human skeletal remains from the Dnieper region of Ukraine (Lillie et al. 2009). The current study outlines the evidence for freshwater resource exploitation throughout the period ~10,200–3700 cal BC, and presents the available evidence for the existence of dietary offsets in the ¹⁴C dates obtained. We have obtained human skeletal material from 54 Epipaleolithic to Mesolithic period individuals and 267 Neolithic to Eneolithic individuals, from 13 cemeteries, since our research in Ukraine began in 1992. Here, we present the initial results of stable isotope analysis of Eneolithic individuals from the Igren VIII cemetery alongside the Epipaleolithic to Eneolithic samples that have previously been analyzed. When contrasted against the evidence from the prehistoric fauna and fish remains studied, and modern fish species from the Dnieper region, we continue to see variability in diets at the population level, both internally and across cemeteries. We also observed temporal variability in human diets across these chronological periods. The fish samples (both archaeological and modern) show a wide range of isotope ratios for both δ¹³C and δ¹⁵N, which could prove significant when interpreting the dietary sources being exploited. This information directly informs the ¹⁴C dating program as an inherent degree of complexity is introduced into the dating of individuals whose diets combine freshwater and terrestrial sources in differing quantities and at differing temporal and/or spatial scales (e.g. Bronk Ramsey et al. 2014

AMS radiocarbon dating from the Neolithic of eastern Ukraine casts doubts on existing chronologies

The Seversky Donets River (Northern Donets) basin in eastern Ukraine and the Lower Don River valley in Russia were inhabited by populations that have been considered to be one of the earliest pottery-using cultures in Europe. The early pottery sites are all located on riverbanks and contain middens with many mollusk shells and fish bones. This suggests the intense exploitation of freshwater resources. The accuracy of radiocarbon dates obtained from these locations is of crucial importance for understanding the development of new technologies, diversification of the food consumed and its preparation strategies, as well as the degree of sedentism in this region, associated with the beginnings of pottery-making technology. The chronology of Neolithic sites in this region, however, was developed on the basis of 14C dates commonly obtained through the dating of freshwater mollusk shells, pottery with mollusk shell temper, or organic residue on pottery shards. Such samples are potentially affected by the freshwater reservoir effect, raising concerns about the accuracy of those dates. This paper presents accelerator mass spectrometry (AMS) 14C dates from a small pilot study from sites in eastern Ukraine in order to test for the presence of the reservoir effect in this region. The AMS 14C dates presented in this paper challenge the 14C chronology based on mollusk shell or organic residue, which appears to generate much older dates than those on wood charcoal or terrestrial animal bone

Diet uniformity at an early farming community in northwest Anatolia (Turkey) : carbon and nitrogen isotope studies of bone collagen at Aktopraklik

Aktopraklık is a settlement site composed of three areas (A–C) in the Marmara region of northwest Anatolia, with phases of occupation that date to the Late Neolithic and Early Chalcolithic periods, mid-seventh to mid-sixth millennium bc (ca. 6400–5600 cal. bc). Here, we present 54 human and fauna bone collagen stable isotope results from the site, alongside five modern fish bone collagen isotope results, to examine the nature of human diet. The stable isotope analysis shows that human diet comprised the consumption of select C3 terrestrial resources, with a preference for domestic animal proteins over plant proteins. The evidence to date suggests that animal husbandry was at the forefront of Late Neolithic and Early Chalcolithic subsistence practices. No isotopic difference in humans is observed between biological sex or between areas B and C at the settlement

First evidence for interpersonal violence in Ukraine's Trypillian farming culture : Individual 3 from Verteba Cave, Bilche Zolote

This paper presents the initial stages of an interdisciplinary study of human skeletal remains interred at Verteba Cave, western Ukraine. This site has been described previously as a “ritual site of the Trypillian culture complex” by Nikitin et al. in Comprehensive site chronology and ancient mitochondrial DNA analysis from Verteba Cave – a Trypillian culture site of Eneolithic Ukraine, Interdisciplinaria Archaeologica: Natural Sciences in Archaeology 1, 9–18., and the material considered here is one of seven crania recovered during excavations at Verteba between 2008 and 2010. Palaeopathological analysis of the individual considered here indicates that this is a young adult female with evidence for peri-mortem injury, cranial surgery and into early stage Trypillia culture inter-personal interactions and burial ritual in this region of Ukraine. Paper published in Proceedings of the British Association for Biological Anthropology and Osteoarchaeology 13th and 14th Annual Conferences in Edinburgh (2nd-4th September 2011) and Bournemouth (14th–16th September 2012)

Mitochondrial DNA analysis of eneolithic trypillians from Ukraine reveals neolithic farming genetic roots

The agricultural revolution in Eastern Europe began in the Eneolithic with the Cucuteni-Trypillia culture complex. In Ukraine, the Trypillian culture (TC) existed for over two millennia (ca. 5,400–2,700 BCE) and left a wealth of artifacts. Yet, their burial rituals remain a mystery and to date almost nothing is known about the genetic composition of the TC population. One of the very few TC sites where human remains can be found is a cave called Verteba in western Ukraine. This report presents four partial and four complete mitochondrial genomes from nine TC individuals uncovered in the cave. The results of this analysis, combined with the data from previous reports, indicate that the Trypillian population at Verteba carried, for the most part, a typical Neolithic farmer package of mitochondrial DNA (mtDNA) lineages traced to Anatolian farmers and Neolithic farming groups of central Europe. At the same time, the find of two specimens belonging to haplogroup U8b1 at Verteba can be viewed as a connection of TC with the Upper Paleolithic European populations. At the level of mtDNA haplogroup frequencies, the TC population from Verteba demonstrates a close genetic relationship with population groups of the Funnel Beaker/ Trichterbecker cultural complex from central and northern Europe (ca. 3,950–2,500 BCE)

Prva analiza izotopov in novi radiokarbonski datumi poljedelcev kulture Tripolje iz jame Verteba, Bilche Zolote, Ukrajina

This paper presents an analysis of human and animal remains from Verteba cave, near Bilche Zolote, western Ukraine. This study was prompted by a paucity of direct dates on this material and the need to contextualise these remains in relation both to the transition from hunting and gathering to farming in Ukraine, and their specific place within the Cucuteni-Trypillia culture sequence. The new absolute dating places the remains studied here in Trypillia stages BII/CI at c. 3900–3500 cal BC, with one individual now redated to the Early Scythian period. As such, these finds are even more exceptional than previously assumed, being some of the earliest discovered for this culture. The isotope analyses indicate that these individuals are local to the region, with the dietary stable isotopes indicating a C3 terrestrial diet for the Trypillia-period humans analysed. The Scythian period individual has δ13C ratios indicative of either c. 50% marine, or alternatively C4 plant inputs into the diet, despite δ18O and 87Sr/86Sr ratios that are comparable to the other individuals studied.V članku predstavljamo analizo človeških in živalskih ostankov iz jame Verteba blizu kra­ja Bilche Zolote v zahodni Ukrajini. Študija je nastala zaradi pomanjkljivega števila datumov iz teh ostankov in iz potrebe po kontekstualiziranju najdb v odnosu tako do prehoda iz lovsko-nabiral­niške družbe v poljedelsko v Ukrajini kot tudi glede na poseben prostor, ki ga imajo te najdbe znotraj kulturne sekvence Cucuteni-Tripolje. Novi absolutni datumi postavljajo ostanke faze BII/CI kulture Tripolje v čas ok. 3900–3500 pr. n. št., medtem ko je eden od pokopov zdaj ponovno datiran v čas zgod­­njega skitskega obdobja, kar pomeni, da gre za ene najstarejših najdb te kulture. Analiza izoto­pov kaže, da so bili pokojni lokalni prebi­val­ci regije, analiza stabilnih izotopov pa kaže na C3 ko­pen­sko prehrano ljudi v času kulture Tri­po­lje. Pokop iz skitskega obdobja kaže glede na δ13C vredno­sti na ok. 50 % morske prehrane, oziroma na C4 rastline, vključene v prehrano, kljub temu da so deleži izotopov δ18O in 87Sr/86Sr primerljivi s preo­sta­limi vzorci ljudi na najdišču

Procalcitonin Is Not a Reliable Biomarker of Bacterial Coinfection in People With Coronavirus Disease 2019 Undergoing Microbiological Investigation at the Time of Hospital Admission

Admission procalcitonin measurements and microbiology results were available for 1040 hospitalized adults with coronavirus disease 2019 (from 48 902 included in the International Severe Acute Respiratory and Emerging Infections Consortium World Health Organization Clinical Characterisation Protocol UK study). Although procalcitonin was higher in bacterial coinfection, this was neither clinically significant (median [IQR], 0.33 [0.11–1.70] ng/mL vs 0.24 [0.10–0.90] ng/mL) nor diagnostically useful (area under the receiver operating characteristic curve, 0.56 [95% confidence interval, .51–.60])

Co-infections, secondary infections, and antimicrobial use in patients hospitalised with COVID-19 during the first pandemic wave from the ISARIC WHO CCP-UK study: a multicentre, prospective cohort study

Background: Microbiological characterisation of co-infections and secondary infections in patients with COVID-19 is lacking, and antimicrobial use is high. We aimed to describe microbiologically confirmed co-infections and secondary infections, and antimicrobial use, in patients admitted to hospital with COVID-19. Methods: The International Severe Acute Respiratory and Emerging Infections Consortium (ISARIC) WHO Clinical Characterisation Protocol UK (CCP-UK) study is an ongoing, prospective cohort study recruiting inpatients from 260 hospitals in England, Scotland, and Wales, conducted by the ISARIC Coronavirus Clinical Characterisation Consortium. Patients with a confirmed or clinician-defined high likelihood of SARS-CoV-2 infection were eligible for inclusion in the ISARIC WHO CCP-UK study. For this specific study, we excluded patients with a recorded negative SARS-CoV-2 test result and those without a recorded outcome at 28 days after admission. Demographic, clinical, laboratory, therapeutic, and outcome data were collected using a prespecified case report form. Organisms considered clinically insignificant were excluded. Findings: We analysed data from 48 902 patients admitted to hospital between Feb 6 and June 8, 2020. The median patient age was 74 years (IQR 59–84) and 20 786 (42·6%) of 48 765 patients were female. Microbiological investigations were recorded for 8649 (17·7%) of 48 902 patients, with clinically significant COVID-19-related respiratory or bloodstream culture results recorded for 1107 patients. 762 (70·6%) of 1080 infections were secondary, occurring more than 2 days after hospital admission. Staphylococcus aureus and Haemophilus influenzae were the most common pathogens causing respiratory co-infections (diagnosed ≤2 days after admission), with Enterobacteriaceae and S aureus most common in secondary respiratory infections. Bloodstream infections were most frequently caused by Escherichia coli and S aureus. Among patients with available data, 13 390 (37·0%) of 36 145 had received antimicrobials in the community for this illness episode before hospital admission and 39 258 (85·2%) of 46 061 patients with inpatient antimicrobial data received one or more antimicrobials at some point during their admission (highest for patients in critical care). We identified frequent use of broad-spectrum agents and use of carbapenems rather than carbapenem-sparing alternatives. Interpretation: In patients admitted to hospital with COVID-19, microbiologically confirmed bacterial infections are rare, and more likely to be secondary infections. Gram-negative organisms and S aureus are the predominant pathogens. The frequency and nature of antimicrobial use are concerning, but tractable targets for stewardship interventions exist. Funding: National Institute for Health Research (NIHR), UK Medical Research Council, Wellcome Trust, UK Department for International Development, Bill & Melinda Gates Foundation, EU Platform for European Preparedness Against (Re-)emerging Epidemics, NIHR Health Protection Research Unit (HPRU) in Emerging and Zoonotic Infections at University of Liverpool, and NIHR HPRU in Respiratory Infections at Imperial College London