Sensorfusion und Regelung eines Roboters am kontinuierlich bewegten Band

Der Artikel beschreibt die Montage eines Anbauteils an ein kontinuierlich bewegtes Basisbauteil, dessen Bewegung durch unterschiedliche Sensoren gemessen wird. Dabei werden u. a. die Ergebnisse einer Bildverarbeitung mit den Auslenkungen eines nachgiebigen Kraft- Momentensensors fusioniert. Aus den Bahnpunkten wird dann eine geglättete Solltrajektorie für das Anbauteil bestimmt. Die Regelung des Roboters wird durch Vorsteuerungen ergänzt, die die dynamischen Verzögerungen und Schwingungen von Roboter und Endeffektor prädiktiv kompensieren

Growth Hormone (GH)-Releasing Peptide Stimulation of GH Release from Human Somatotroph Adenoma Cells: Interaction with GH-Releasing Hormone, Thyrotropin- Releasing Hormone, and Octreotide.

The synthetic hexapeptide GH-releasing peptide (GHRP; His-D-Trp-Ala-Trp-D-Phe-Lys-NH2) specifically stimulates GH secretion in humans in vivo and in animals in vitro and in vivo via a still unknown receptor and mechanism. To determine the effect of GHRP on human somatotroph cells in vitro, we stimulated cell cultures derived from 12 different human somatotroph adenomas with GHRP alone and in combination with GH-releasing hormone (GHRH), TRH, and the somatostatin analog octreotide. GH secretion of all 12 adenoma cultures could be stimulated with GHRP, whereas GHRH was active only in 6 adenoma cultures. In GHRH-responsive cell cultures, simultaneous application of GHRH and GHRP had an additive effect on GH secretion. TRH stimulated GH release in 4 of 7 adenoma cultures; in TRH-responsive cell cultures there was also an additive effect of GHRP and TRH on GH secretion. In 5 of 9 adenoma cultures investigated, octreotide inhibited basal GH secretion. In these cell cultures, GHRP-induced GH release was suppressed by octreotide. In 5 of 5 cases, the protein kinase-C inhibitor phloretin partly inhibited GHRP-stimulated GH release, but not basal GH secretion. In summary, GH secretion was stimulated by GHRP in all somatotroph adenomas investigated, indicating that its unknown receptor and signaling pathway are expressed more consistently in somatotroph adenoma cells than those for GHRH, TRH, and somatostatin. Our data give further evidence that GHRP-stimulated GH secretion is mediated by a receptor different from that for GHRH or TRH, respectively, and that protein kinase-C is involved in the signal transduction pathway. Because human somatotroph adenoma cell cultures respond differently to various neuropeptides (GHRH, TRH, somatostatin, and others), they provide a model for further investigation of the mechanism of action of GHRP-induced GH secretion

Performanzsteigerung durch Selektivität? Erwartbare Effekte von Forschungsevaluationen an deutschen Universitäten im Lichte internationaler Erfahrungen

Während der vergangenen drei Jahrzehnte hat sich die Bewertung der Performanz öffentlich alimentierter Forschung zu einem wichtigen Thema der Wissenschaftspolitik in den OECD-Ländern entwickelt. Viele Länder haben Forschungsevaluationssysteme eingeführt, um die Qualität der Forschungs-Outputs an ihren Universitäten zu messen, zu beurteilen und in Rangskalen zu listen. Die entsprechenden Entwicklungen in Deutschland sind durch einen späten Beginn und ausgeprägte föderale Fragmentierung gekennzeichnet. Der Beitrag beschreibt zunächst die jüngsten deutschen Ansätze zur Einführung mehrerer Forschungsevaluationssysteme auf verschiedenen Politikebenen. Danach werden die Vorreiter-Evaluationssysteme Großbritanniens, der Niederlande und Australiens untersucht und mit Blick auf ihre Effekte auf Universitätsmanagement sowie Forschung und Lehre diskutiert. Dabei interessiert insbesondere die Frage, inwiefern intendierte und nicht-intendierte Effekte dieser Evaluationssysteme für die Einführung und aktuelle Entwicklung der deutschen Ansätze instruktiv sind. Es zeigt sich, dass die deutschen Systeme der Evaluation von Forschungsperformanz zahlreiche Ähnlichkeiten mit denen der Vorreiter-Länder haben und vor allem von den negativen und nicht-intendierten Effekten lernen können, die sich dort in einer über zwanzigjährigen Evaluationspraxis und deren Folgen gezeigt haben.During the past three decades the performance assessment of state-financed research has become a major issue of science policies in OECD countries. Accordingly, many countries started to introduce research evaluation systems (RES) to assess and reward the performance of research in their universities. Germany is a latecomer in this respect. Owing to its federal constitution, a multitude of RES has emerged. This article aims at drawing conclusions about likely intended and unintended effects of the recently introduced German RES from an analysis of first-mover-RES in Great Britain, the Netherlands, and Australia. The article describes recent German attempts to establish several RES at different policy levels, and summarizes effects of the British, Dutch and Australian RES on university management as well as teaching and research. It turns out that German RES bear certain similarities with the first-mover-systems, which enables conclusions concerning the likelihood of negative effects which have shown up in more than twenty years of evaluations abroad

Diagnostic accuracy of C-reactive protein and white blood cell counts in the early detection of inflammatory complications after open resection of colorectal cancer: a retrospective study of 1,187 patients

Purpose: Although widely used, there is a lack of evidence concerning the diagnostic accuracy of C-reactive protein (CRP) and white blood cell counts (WBCs) in the postoperative period. The aim of this study was to evaluate the diagnostic accuracy of CRP and WBCs in predicting postoperative inflammatory complications after open resection of colorectal cancer. Methods: In this retrospective study, clinical data and the CRP and WBCs, routinely measured until postoperative day5 (POD 5), were available for 1,187 patients who underwent colorectal cancer surgery between 1997 and 2009. Using the receiver-operating characteristic (ROC) methodology, the diagnostic accuracy was evaluated according to the area under the curve (AUC). Results: Three hundred forty-seven patients (29.2%; 95% CI, 26.7-31.9%) developed various inflammatory complications. Anastomotic leakage occurred in 8.0% (95% CI, 6.1-9.1%) of patients. The CRP level on POD 4 (AUC 0.76; 95% CI, 0.71-0.81) had the highest diagnostic accuracy for the early detection of inflammatory complications. With a cutoff of 123mg/l, the sensitivity was 0.66 (95% CI, 0.56-0.74), and the specificity was 0.77 (95% CI, 0.71-0.82). The diagnostic accuracy of the WBC was significantly lower compared to CRP. Conclusion: Measurement of CRP on POD 4 is recommended to screen for inflammatory complications. CRP values above 123mg/l on POD 4 should raise suspicion of inflammatory complications, although the discriminatory performance was insufficient to provide a single threshold that could be used to correctly predict inflammatory complications in clinical practice. WBC measurement contributes little to the early detection of inflammatory complications. Registered at www.clinicaltrials.gov (NCT01221324

Risk Factors for Anastomotic Leakage after Rectal Cancer Resection and Reconstruction with Colorectostomy. A Retrospective Study with Bootstrap Analysis

Background: This study was designed to apply modern statistical methods to evaluate risk factors for anastomotic leakage after rectal cancer resection in a retrospective cohort of patients who received a colorectostomy. Whereas a diverting stoma and tumor height are considered proven risk factors for anastomotic leakage, a lack of evidence about additional risk factors persists. Methods: In a single-center study, 527 consecutive patients who received a colorectostomy after rectal cancer resection between 1991 and 2008 were retrospectively assessed. In addition to traditional uni- and multivariate regression, locally weighted scatterplot smoothing (LOWESS) regression and bootstrap analysis were applied to increase internal validity. Results: Anastomotic leakage occurred in 70 patients (13.3%; 95% confidence interval (CI), 10.5-16.5%) and mortality was 2.5% (95% CI, 1.4-4.2%). Diverting stoma (odds ratio (OR), 0.4; 95% CI, 0.17-0.61) and tumor height (OR, 0.88; 95% CI, 0.8-0.94) were proven to be protective. Neoadjuvant radiotherapy (OR, 2.15; 95% CI, 1.58-4.24) and intraoperative blood loss (OR, 1.05; 95% CI, 1.02-1.09) had a derogatory effect. Bootstrap analysis identified pre-existing vascular disease (95.5%), more advanced UICC stage III or IV tumors (95.7% or 91.5%, respectively), and intraoperative (96.1%) and postoperative (99.4%) blood substitution as harmful. Both intraoperative and postoperative blood substitution caused a dose-dependent increase in risk. Conclusions: Applying statistical resampling methods identified intraoperative blood loss, blood substitution, vascular disease, and advanced UICC stage as risk factors for anastomotic leakage. Greater distances between the tumor and the anal verge and performance of a diverting stoma were associated with a decreased risk of anastomotic leakag

Structure analysis of the protein translocating channel TatA in membranes using a multi-construct approach

AbstractThe twin-arginine-translocase (Tat) can transport proteins in their folded state across bacterial or thylakoid membranes. In Bacillus subtilis the Tat-machinery consists of only two integral (inner) membrane proteins, TatA and TatC. Multiple copies of TatA are supposed to form the transmembrane channel, but little structural data is available on this 70-residue component. We used a multi-construct approach for expressing several characteristic fragments of TatAd, to determine their individual structures and to cross-validate them comprehensively within the architecture of the full-length protein. Here, we report the design, high-yield expression, detergent-aided purification and lipid-reconstitution of five constructs of TatAd, overcoming difficulties associated with the very different hydrophobicities and sizes of these membrane protein fragments. Circular dichroism (CD) and oriented CD (OCD) were used to determine their respective conformations and alignments in suitable, negatively charged phospholipid bilayers. CD spectroscopy showed an N-terminal α-helix, a central helical stretch, and an unstructured C-terminus, thus proving the existence of these secondary structures in TatAd for the first time. The OCD spectra demonstrated a transmembrane orientation of the N-terminal α-helix and a surface alignment of the central amphiphilic helix in lipid bilayers, thus supporting the postulated topology model and function of TatA as a transmembrane channel

Stretching of polymers in a turbulent environment

The interaction of polymers with small-scale velocity gradients can trigger a coil-stretch transition in the polymers. We analyze this transition within a direct numerical simulation of shear turbulence with an Oldroyd-B model for the polymer. In the coiled state the lengths of polymers are distributed algebraically with an exponent alpha=2 gamma-1/De, where gamma is a characteristic stretching rate of the flow and De the Deborah number. In the stretched state we demonstrate that the length distribution of the polymers is limited by the feedback to the flow