Barrier function and cell protection of the intestinal epithelium. Applications to the pig during weaning

This review focuses on recent progress on the understanding of intestinal barrier function and epithelial cell protection by heat shock proteins (HSP). Barrier function regulation and its importance for the swine species are presented. Major progress in the understanding of gut barrier function and epithelial cell protection and their regulation has been made recently. Tight junctions and HSP appear to have a major role. These defence systems are highly dynamic, integrated and finely regulated at the epithelial monolayer. The first level of regulation is a direct cross-talk between the gut microbiota and the host. The second level involves the nervous system, mucosal mast cells and various mediators acting on the epithelium. Gut barrier function and its regulation can be altered by stress and disturbances of the microbiota. Additional studies need to be conducted in swine for developing appropriate strategies for preventing post-weaning gut disordersCette revue fait le point sur les récents progrès dans la compréhension de la fonction de barrière intestinale et de la cytoprotection épithéliale par les protéines du choc thermique (HSP). La régulation de la fonction de barrière et son importance pour l’espèce porcine sont présentées. Des progrès importants dans la compréhension de la fonction de barrière et de la cytoprotection ont été réalisés récemment. Les jonctions serrées et les HSP jouent un rôle majeur. Ces systèmes de protection sont hautement dynamiques, intégrés et finement régulés au niveau de l’épithélium intestinal. Le premier niveau de régulation est exercé par le microbiote. Le second niveau implique le système nerveux, le mastocyte et divers médiateurs agissant sur l’épithélium. La barrière est influencée par le stress ou par des perturbations du microbiote. Des études complémentaires méritent d’être conduites chez le porc pour définir des stratégies alimentaires et d’élevage destinées à limiter les perturbations digestives du sevrag

Analisis Brand Equity Pada Produk Sepatu Sport Di Online Shop

This study aims to to analyze brand awareness ,brand association, perception of the qualities ( perceived quality and their brand loyalty) of their products shoes sport in online shop. The population of the study are all customers shoes sport in online shop on students FEB Muhammadiyah University Surakarta. The research sample this is 100 customers shoes sport in online shop on students FEB Muhammadiyah University Surakarta. The research sample this is 100 customers shoes sport in online shop on students FEB Muhammadiyah University Surakarta. Analysis of data was undertaken with an analysis brand equity consisting of the trial differensial, semantic scale cochran, test Importance-Performance Analysis ( IPA) brand switching his patterns and the matrix. Based on the research done be seen that a brand that to be top of mind through to the customer is nike brand, sport shoes that was followed by the adidas brand, reebok and brands other. sport shoesBased on measurements on brand perceived quality got that third, sport that is nike branded shoes adidas, and reebok not substantially.These feelings are reflected in an average rate of approaching the same for all attributes the lab test.But the attribute of being the highest value in the eyes of customers for the third out to reposition the brand is on an attribute availability.In the form of associations asosociation brand products shoes sport is their prices affordable save to use high in quality, as well as ease of getting this product in a shop online. The results of brand loyalty of all three branded shoes sport can be determined with manifest to all customers loyal with the buy benar-benar kebiasaaan because that can be identified with a pattern of a pembeliannya. beruntunan together Keywords: awareness , association, perceptions and loyalt

Maternal Antibiotic-Induced Early Changes in Microbial Colonization Selectively Modulate Colonic Permeability and Inducible Heat Shock Proteins, and Digesta Concentrations of Alkaline Phosphatase and TLR-Stimulants in Swine Offspring

Elevated intake of high energy diets is a risk factor for the development of metabolic diseases and obesity. High fat diets cause alterations in colonic microbiota composition and increase gut permeability to bacterial lipopolysaccharide, and subsequent low-grade chronic inflammation in mice. Chronic inflammatory bowel diseases are increasing worldwide and may involve alterations in microbiota-host dialog. Metabolic disorders appearing in later life are also suspected to reflect changes in early programming. However, how the latter affects the colon remains poorly studied. Here, we hypothesized that various components of colonic physiology, including permeability, ion exchange and protective inducible heat shock proteins (HSP) are influenced in the short- and long-terms by early disturbances in microbial colonization. The hypothesis was tested in a swine model. Offspring were born to control mothers (n = 12) or mothers treated with the antibiotic (ATB) amoxicillin around parturition (n = 11). Offspring were slaughtered between 14 and 42 days of age to study short-term effects. For long-term effects, young adult offspring from the same litters consumed a normal or a palm oil-enriched diet for 4 weeks between 140 and 169 days of age. ATB treatment transiently modified maternal fecal microbiota although the minor differences observed for offspring colonic microbiota were nonsignificant. In the short-term, consistently higher HSP27 and HSP70 levels and transiently increased horseradish peroxidase permeability in ATB offspring colon were observed. Importantly, long-term consequences included reduced colonic horseradish peroxidase permeability, and increased colonic digesta alkaline phosphatase (AP) and TLR2- and TLR4-stimulant concentrations in rectal digesta in adult ATB offspring. Inducible HSP27 and HSP70 did not change. Interactions between early ATB treatment and later diet were noted for paracellular permeability and concentrations of colonic digesta AP. In conclusion, our data suggest that early ATB-induced changes in bacterial colonization modulate important aspects of colonic physiology in the short- and longterms

Nutrition and gut health of the young pig around weaning: what news?

International audienc

Biology, environmental and nutritional modulation of skin mucus alkaline phosphatase in fish. A review

Alkaline phosphatase (AP) is a major, recently recognized component of innate immunity. The intestinal AP (IAP) isoform plays a pivotal role in controlling gastrointestinal and systemic inflammation in terrestrial mammals. This is so essentially through detoxification (by dephosphorylation) of proinflammatory microbial components that can no longer be recognized by so-called toll-like receptors, thus preventing cellular inflammatory cascade activation. A unique feature of fish is the presence of AP in skin and epidermal mucus (skin mucus AP) but its actual functions and underlying mechanisms of action are presently unknown. Here, we gather and analyse knowledge available on skin mucus AP in order to provide a holistic view of this important protective enzyme. Our main conclusions are that skin mucus AP is responsive to biotic and abiotic factors, including nutrients and bioactive feed components, prebiotics and probiotics. Importantly, both skin mucus AP and IAP appear to correlate, thus raising the interesting possibility that skin mucus AP be used as a proxy for IAP in future nutritional studies. Blood serum AP also seems to correlate with skin mucus AP, though biological interpretation for such relationship is presently unknown. Finally, the precise isoform/s of AP present in skin should be identified and underlying molecular mechanisms of skin mucus AP actions deciphered

Some aspects of digestive disorders of dietary origin in calves and lambs

International audienc

Basis and regulation of gut barrier function and epithelial cytoprotection: applications to the weaned pig

Conférence organisée par Schothorst Feed ResearchInternational audienc

Allergenic and antigenic factors in soyabean

International audienc

Soy products as protein sources for preruminants and young pigs

International audienc

The EU project Healthypigut (2001-2004) Workshop 1 - Digestive physiology, microbiology and immunology basis for gut function and dysfunction in pigs - Foreword

International audienc