10,304 research outputs found
Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes
A 43 × 10^3 M_r protein (designated connexin43 or Cx43) is a major constituent of heart gap junctions. The understanding of its arrangement in junctional membranes has been extended by means of site-directed antibodies raised against synthetic peptides of Cx43. These represent part of the first extracellular loop (EL-46), the cytoplasmic loop (CL-100), the second extracellular loop (EL-186) and carboxy-terminal sequences (CT-237 and CT-360). All of the antibodies raised reacted with their respective peptides and the Cx43 protein on Western blots. By immunoelectron microscopy two of the antibodies (CL-100 and CT-360) were shown to label the cytoplasmic surface of isolated gap junction membranes. Immunofluorescent labeling at locations of neonatal cardiac myocyte-myocyte apposition required an alkali/urea treatment when the EL-46 and EL-186 antibodies were used. Immunoblot analysis of endoproteinase Lys-C-digested gap junctions revealed that the Cx43 protein passed through the lipid bilayer four times. Alkaline phosphatase digestion of isolated junctions was used to show that the CT-360 antibody recognized many phosphorylated forms of Cx43. Our results unequivocally confirm models of the organization of Cx43 that were based on a more limited set of data and a priori considerations of the sequence
Fresh-Register Automata
What is a basic automata-theoretic model of computation with names and fresh-name generation? We introduce Fresh-Register Automata (FRA), a new class of automata which operate on an infinite alphabet of names and use a finite number of registers to store fresh names, and to compare incoming names with previously stored ones. These finite machines extend Kaminski and Francez’s Finite-Memory Automata by being able to recognise globally fresh inputs, that is, names fresh in the whole current run. We exam-ine the expressivity of FRA’s both from the aspect of accepted languages and of bisimulation equivalence. We establish primary properties and connections between automata of this kind, and an-swer key decidability questions. As a demonstrating example, we express the theory of the pi-calculus in FRA’s and characterise bisimulation equivalence by an appropriate, and decidable in the finitary case, notion in these automata
Excitation Transfer Collisions And Electron Seeding Processes In A Resonantly Excited Sodium Vapor
A dense sodium vapor in a high-pressure buffer of argon has been simultaneously excited by short (4 ns) laser pulses from two lasers: the first tuned to one of the D line transitions at 589 nm and the second tuned to the photoionization threshold of the 3p states near 406 nm. The temporal evolution of the system was studied with and without the photoionizing laser pulses. At early times (∼100 ns) excited state populations are determined by energy transfer collisions between two laser-excited 3p atoms while the ion/electron density is controlled by super elastic heating of seed electrons followed by electron impact ionization of excited state atoms. At late times (∼ 1 μs) excited state populations are controlled by collisional- radiative recombination processes. Excitation transfer rates into the 4d, 5d, 6d, and 6s levels are measured. © 1981 American Institute of Physics
Electron Density Measurements In A Laser Induced Na Plasma
The optical emission spectrum of a recombining Na plasma is observed. The shift and broadening of the spectral line emission due to electron collisions is observed. Electron densities on the order of 5x1014 cm -3 are calculated from the shift measurements. The results agree well with electron densities obtained in the same system from Saha plots of excited state densities. © 1982 American Institute of Physics
Rare-gas, Collision-induced Shifts Of The ND States Of Sodium
We have measured line shifts of the 3P-nD transitions in sodium induced by collisions with helium, argon, and krypton for n=5 to 18. The shifts of the diffuse series transitions increase rapidly with principal quantum number and asymptotically approach the limit predicted by the Fermi model at high quantum numbers. © 1982 The American Physical Society
The X-ray luminosity function of AGN at z~3
We combine Lyman-break colour selection with ultradeep (> 200 ks) Chandra
X-ray imaging over a survey area of ~0.35 deg^2 to select high redshift AGN.
Applying careful corrections for both the optical and X-ray selection
functions, the data allow us to make the most accurate determination to date of
the faint end of the X-ray luminosity function (XLF) at z~3. Our methodology
recovers a number density of X-ray sources at this redshift which is at least
as high as previous surveys, demonstrating that it is an effective way of
selecting high z AGN. Comparing to results at z=1, we find no evidence that the
faint slope of the XLF flattens at high z, but we do find significant (factor
~3.6) negative evolution of the space density of low luminosity AGN. Combining
with bright end data from very wide surveys we also see marginal evidence for
continued positive evolution of the characteristic break luminosity L*. Our
data therefore support models of luminosity-dependent density evolution between
z=1 and z=3. A sharp upturn in the the XLF is seen at the very lowest
luminosities (Lx < 10^42.5 erg s^-1), most likely due to the contribution of
pure X-ray starburst galaxies at very faint fluxes.Comment: 16 pages, 9 figures, accepted for publication in MNRA
Adjusting the melting point of a model system via Gibbs-Duhem integration: application to a model of Aluminum
Model interaction potentials for real materials are generally optimized with
respect to only those experimental properties that are easily evaluated as
mechanical averages (e.g., elastic constants (at T=0 K), static lattice
energies and liquid structure). For such potentials, agreement with experiment
for the non-mechanical properties, such as the melting point, is not guaranteed
and such values can deviate significantly from experiment. We present a method
for re-parameterizing any model interaction potential of a real material to
adjust its melting temperature to a value that is closer to its experimental
melting temperature. This is done without significantly affecting the
mechanical properties for which the potential was modeled. This method is an
application of Gibbs-Duhem integration [D. Kofke, Mol. Phys.78, 1331 (1993)].
As a test we apply the method to an embedded atom model of aluminum [J. Mei and
J.W. Davenport, Phys. Rev. B 46, 21 (1992)] for which the melting temperature
for the thermodynamic limit is 826.4 +/- 1.3K - somewhat below the experimental
value of 933K. After re-parameterization, the melting temperature of the
modified potential is found to be 931.5K +/- 1.5K.Comment: 9 pages, 5 figures, 4 table
Measurement of Temporal Correlations of the Overhauser Field in a Double Quantum Dot
In quantum dots made from materials with nonzero nuclear spins, hyperfine
coupling creates a fluctuating effective Zeeman field (Overhauser field) felt
by electrons, which can be a dominant source of spin qubit decoherence. We
characterize the spectral properties of the fluctuating Overhauser field in a
GaAs double quantum dot by measuring correlation functions and power spectra of
the rate of singlet-triplet mixing of two separated electrons. Away from zero
field, spectral weight is concentrated below 10 Hz, with 1/f^2 dependence on
frequency, f. This is consistent with a model of nuclear spin diffusion, and
indicates that decoherence can be largely suppressed by echo techniques.Comment: related papers available at http://marcuslab.harvard.ed
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