Cannabinoid Receptor Involvement in Stress-Induced Cocaine Reinstatement: Potential Interaction with Noradrenergic Pathways

This study examined the role of endocannabinoid signaling in stress-induced reinstatement of cocaine seeking and explored the interaction between noradrenergic and endocannabinergic systems in the process. A well-validated preclinical model for human relapse, the rodent conditioned place preference assay, was used. Cocaine-induced place preference was established in C57BL/6 mice using injections of 15 mg/kg cocaine. Following extinction of preference for the cocaine-paired environment, reinstatement of place preference was determined following 6 min of swim stress or cocaine injection (15 mg/kg, i.p.). The role of endocannabinoid signaling was studied using the cannabinoid antagonist AM-251 (3 mg/kg, i.p.). Another cohort of mice was tested for reinstatement following administration of the cannabinoid agonist CP 55,940 (10, 20, or 40 μg/kg, i.p.). The alpha-2 adrenergic antagonist BRL-44408 (5 mg/kg, i.p.) with or without CP 55,940 (20 μg/kg) was administered to a third group of mice. We found that: (1) AM-251 blocked forced swim-induced, but not cocaine-induced, reinstatement of cocaine-seeking behavior; (2) the cannabinoid agonist CP 55,940 did not reinstate cocaine-seeking behavior when administered alone but did synergize with a non-reinstating dose of the alpha-2 adrenergic antagonist BRL-44408 to cause reinstatement. These results are consistent with the hypothesis that stress exposure triggers the endogenous activation of CB1 receptors and that activation of the endocannabinoid system is required for the stress-induced relapse of the mice to cocaine seeking. Further, the data suggest that the endocannabinoid system interacts with noradrenergic mechanisms to influence stress-induced reinstatement of cocaine-seeking behavior

Sending femtosecond pulses in circles: highly non-paraxial accelerating beams

We use caustic beam shaping on 100 fs pulses to experimentally generate non-paraxial accelerating beams along a 60 degree circular arc, moving laterally by 14 \mum over a 28 \mum propagation length. This is the highest degree of transverse acceleration reported to our knowledge. Using diffraction integral theory and numerical beam propagation simulations, we show that circular acceleration trajectories represent a unique class of non-paraxial diffraction-free beam profile which also preserves the femtosecond temporal structure in the vicinity of the caustic

Coordinated SuperDARN THEMIS ASI observations of mesoscale flow bursts associated with auroral streamers

Nightside auroral zone localized flow channels, typically associated with auroral poleward boundary intensifications and streamers, are an important component of high‐latitude ionospheric plasma dynamics. We investigate the structure of these flow channels using two‐dimensional line‐of‐sight flow observations from the Super Dual Auroral Radar Network (SuperDARN) radars and auroral images from the Time History of Events and Macroscale Interactions during Substorms (THEMIS) ground‐based all‐sky imager (ASI) array. Radar echoes captured <~500 km horizontal distance from the radars were mainly used to detect small‐scale flow structures that would otherwise be missed or poorly resolved in long‐range radar echoes. After identifying 135 auroral streamers in the ASI images at close‐radar capture locations, we examined the associated ionospheric flow data in the radar echoes. Flow bursts and streamers are invariably correlated in all events. The flow bursts are often directed equatorward and appear simultaneously with the streamers. Equatorward flows are located just to the east of the streamers. Less frequently (~10% of the time), a poleward flow enhancement was detected even when a streamer propagated equatorward, the poleward flow enhancement being located to the west of the auroral streamer, or to the east of the equatorward flow enhancement, consistently with the spatial relationship between flow shear and upward field‐aligned currents in plasma sheet flow bursts. The azimuthal width of the flow channel is, on average, ~75 km, and the azimuthal offset of the equatorward flow channel relative to the auroral streamer is ~57 km eastward. This study demonstrates the capability of radar‐imager pairs for identifying the 2‐D structure of localized flows associated with plasma sheet flow bursts. Key Points Structure of flow channels using SuperDARN radars and THEMIS ASI is investigated Simultaneous flow bursts and streamers are invariably correlated in all events 3‐D structure of flows consistent with plasma flow shears around a BBF channelPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106072/1/jgra50744.pd

Real-time full bandwidth measurement of spectral noise in supercontinuum generation

The ability to measure real-time fluctuations of ultrashort pulses propagating in optical fiber has provided significant insights into fundamental dynamical effects such as modulation instability and the formation of frequency-shifting rogue wave solitons. We report here a detailed study of real-time fluctuations across the full bandwidth of a fiber supercontinuum which directly reveals the significant variation in measured noise statistics across the spectrum, and which allows us to study correlations between widely separated spectral components. For two different propagation distances corresponding to the onset phase of spectral broadening and the fully-developed supercontinuum, we measure real time noise across the supercontinuum bandwidth, and we quantify the supercontinuum noise using statistical higher-order moments and a frequency-dependent intensity correlation map. We identify correlated spectral regions within the supercontinuum associated with simultaneous sideband generation, as well as signatures of pump depletion and soliton-like pump dynamics. Experimental results are in excellent agreement with simulations

The 17 March 2013 storm: Synergy of observations related to electric field modes and their ionospheric and magnetospheric Effects

The main phase of the 17 March 2013 storm had excellent coverage from groundâ based instruments and from lowâ and highâ altitude spacecraft, allowing for evaluation of the relations between major storm time phenomena that are often considered separately. The shock impact with its concurrent southward interplanetary magnetic field (IMF) immediately drove dramatic poleward expansion of the poleward boundary of the auroral oval (implying strong nightside reconnection), strong auroral activity, and strong penetrating midlatitude convection and ionospheric currents. This was followed by periods of southward IMF driving of electric fields that were at first relatively smooth as often employed in storm modeling but then became extremely bursty and structured associated with equatorward extending auroral streamers. The auroral oval did not expand much further poleward during these two latter periods, suggesting a lower overall nightside reconnection rate than that during the first period and approximate balance with dayside reconnection. Characteristics of these three modes of driving were reflected in horizontal and fieldâ aligned currents. Equatorward expansion of the auroral oval occurred predominantly during the structured convection mode, when electric fields became extremely bursty. The period of this third mode also approximately corresponded to the time of largest equatorward motion of the ionospheric trough, of apparent transport of high total electron content (TEC) features into the auroral oval from the polar cap, and of largest earthward injection of ions and electrons into the ring current. The enhanced responses of the aurora, currents, TEC, and the ring current indicate a common driving of all these storm time features during the bursty convection mode period.Key PointsStorm had excellent ground/space data coverage, allowing evaluation of relations between major storm phenomena often considered separatelyIdentified three southward IMF electric fields driving modes that were reflected in the aurora and ionospheric and fieldâ aligned currentsThe third mode was extremely bursty, giving common driving of auroral and current structures, TEC changes, and ring current injectionPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135355/1/jgra53033_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/135355/2/jgra53033.pd

A semi-parametric approach to estimate risk functions associated with multi-dimensional exposure profiles: application to smoking and lung cancer

A common characteristic of environmental epidemiology is the multi-dimensional aspect of exposure patterns, frequently reduced to a cumulative exposure for simplicity of analysis. By adopting a flexible Bayesian clustering approach, we explore the risk function linking exposure history to disease. This approach is applied here to study the relationship between different smoking characteristics and lung cancer in the framework of a population based case control study

The NA48 LKr calorimeter digitizer electronics chain

The 13 500 channels of the NA48 liquid-krypton electromagnetic calorimeter readout electronics were put into operation in 1997. The digitizer electronics employs a new gain switching technique that expands the dynamic range of a standard 10-bit ADC to 14 bits at 40 MHz sampling rate employing a custom-developed integrated circuit (KRYPTON). The KRYPTON has been fabricated in 1.2 μm BiCMOS technology and was successfully developed together with industry on a short timescale. The performance and the experience from the first year of the operation of the liquid-krypton calorimeter electronics will also be briefly discussed