Unprecedented silicon(II)-> calcium complexes with N-heterocyclic silylenes

The first N-heterocyclic silylene (NHSi) complexes of any s-block element to date are reported for calcium: [(eta(5)-C5Me5)(2)Ca<--:Si(O-C6H4-2-Bu-t){((NBu)-Bu-t)(2)CPh}] (6) and [(eta(5)-C5Me5)(2)Ca<--:Si((NBuCH)-Bu-t)(2)] (7). Complexes 6 and 7 are isolable in a facile way upon reaction of the corresponding free N-heterocyclic silylenes (NHSis) with [(eta(5)-C5Me5)(2)Ca] (2). Complexes 6 and 7 were fully characterised by spectroscopic means and the single crystal X-ray diffraction analysis of 6 is also reported. Analysis of the bonding situation by DFT methods including a Bader Atoms in molecules (AIM) analysis is also reported. The bonding interaction between the Si and Ca centres in complexes 6 and 7 can best be viewed as sigma-donor-acceptor interactions, with a considerable ionic contribution in the bond. The reactivity towards the oxygen containing substrates THF and benzophenone is also discussed.DFG, EXC 314, Unifying Concepts in Catalysi

Preparing for the unknown:How working memory provides a link between perception and anticipated action

What mechanisms underlie the transfer of a working memory representation into a higher-level code for guiding future actions? Electrophysiological correlates of attentional selection and motor preparation processes within working memory were investigated in two retrospective cuing tasks. In the first experiment, participants stored the orientation and location of a grating. Subsequent feature cues (selective vs. neutral) indicated which feature would be the target for later report. The oscillatory response in the mu and beta frequency range with an estimated source in the sensorimotor cortex contralateral to the responding hand was used as correlate of motor preparation. Mu/beta suppression was stronger following the selective feature cues compared to the neutral cue, demonstrating that purely feature-based selection is sufficient to form a prospective motor plan. In the second experiment, another retrospective cue was included to study whether knowledge of the task at hand is necessary to initiate motor preparation. Following the feature cue, participants were cued to either compare the stored feature(s) to a probe stimulus (recognition task) or to adjust the memory probe to match the target feature (continuous report task). An analogous suppression of mu oscillations was observed following a selective feature cue, even ahead of task specification. Further, a subsequent selective task cue again elicited a mu/beta suppression, which was stronger after a continuous report task cue. This indicates that working memory is able to flexibly store different types of information in higher-level mental codes to provide optimal prerequisites for all required action possibilities

Retroactive Attentional Shifts Predict Performance in a Working Memory Task: Evidence by Lateralized EEG Patterns

Shifts of attention within working memory based on retroactive (retro-) cues were shown to facilitate performance in working memory tasks. Although posterior asymmetries in the EEG, such as the contralateral delay activity (CDA), have been used to study the active storage of lateralized working memory representations, results on the relation of such asymmetric effects to retro-cue benefits remain inconclusive. We recorded EEG in a retro-cue working memory task with lateralized items and a continuous performance response. Following either a selective or neutral retro-cue, participants adjusted the orientation of a central memory probe to the cued item. Selective retro-cues elicited an early posterior contralateral negativity (PCN), anterior directing attention negativity (ADAN) and a later modulation of CDA indicating that active storage was concentrated on the cued information. By dividing all trials into three within-condition performance quantiles, we could further show that high working memory accuracy was associated with a sustained increase of the CDA effect following the retro-cue. These results suggest that focusing resources on the active storage of relevant representations is an important factor regarding retro-cue benefits in working memory tasks

Algorithme de dépliement de phase guidé par un facteur de qualité en holographie numérique pour l'étude des écoulements complexes

L'holographie numérique est une technique optique d'imagerie permettant d'enregistrer simultanément l'amplitude et la phase d'une onde diffractée par un objet. C'est un procédé non intrusif utilisé dans de nombreux domaines biomédicales ou encore technologiques, et dont les applications sont variées : stockage de l'information, analyse de vibrations, étude des tissus biologiques ... En mécanique des fluides, cette technique présente un caractère particulièrement pertinent pour la tomographie d'écoulements puisqu'elle permet de remonter à la distribution bidimensionnelle, voire tridimensionnelle de la masse volumique. Comme pour d'autres techniques d'imagerie impliquant des mesures de phase (imagerie par résonance magnétique, l'imagerie radar à synthèse d'ouverture interférométrique...), en l'holographie numérique, les valeurs de la phase déduites sont contenues dans l'intervalle [- ?, + ?]. Le dépliement de ces phases constitue un point fondamental auquel il faire face pour pouvoir être exploité. C'est une opération non triviale, et le signal mesuré est souvent entaché de bruit ce qui peut être critique dans le cas de milieux à forts gradients d'indice. Le champ de recherche associé a donné lieu à divers travaux et au développement de plusieurs approches de dépliement de phase plus ou moins efficaces. Dans ce contexte de tomographie en mécanique des fluides, un banc d'holographie numérique basé sur un interféromètre de Mach Zehnder hors axe, et développé à l'ONERA [1], a été déployé autour de la soufflerie à rafale de l'Institut Saint-Louis (ISL) pour analyser un écoulement supersonique à Mach 2 autour d'un spike axisymétrique en dérapage. Le spike est en incidence de 6° et effectue un mouvement de rotation autour de l'axe du vent à intervalle régulier pour constituer différentes lignes de visée. Pour chaque position, une série d'interférogrammes est enregistrée avant et pendant l'écoulement. Les cartographies des distributions de phase sont déduites en sélectionnant l'ordre +1 de la transformée de Fourier bidimensionnelle des interférogrammes, et la référence soustraite de la mesure afin de ne conserver que la contribution de l'écoulement dans la phase. Enfin, cette différence de phase résultante est dépliée à l'aide de l'algorithme proposé par Herraez et al. [2] qui suit un chemin non continu défini par un facteur de fiabilité, et qui permet d'isoler et de contourner les zones de choc, souvent problématiques. Cette méthode a été éprouvée et a montré son efficacité sur d'autres types d'écoulements compressibles, avec notamment l'exemple d'un jet d'air sous détendu à différents rapports de pression. Les résultats obtenus dans le cas du spike sont prometteurs et en bon accord avec ceux prédits par les tables de choc.   [1] J.M. Desse, F. Olchewsky, Digital Mach-Zehnder holographic interferometer using pulsed laser for analyzing large flow fields, Digital Holography, Heidelberg, OSA congress, Germany, July 2016. [2] M. A. Herraez, D. R. Burton, M. J. Lalor and M. A. Gdeisat, Applied Optics, Vol. 41, No. 35, (2002

Using ecological and field survey data to establish a national list of the wild bee pollinators of crops

The importance of wild bees for crop pollination is well established, but less is known about which species contribute to service delivery to inform agricultural management, monitoring and conservation. Using sites in Great Britain as a case study, we use a novel qualitative approach combining ecological information and field survey data to establish a national list of crop pollinating bees for four economically important crops (apple, field bean, oilseed rape and strawberry). A traits data base was used to establish potential pollinators, and combined with field data to identify both dominant crop flower visiting bee species and other species that could be important crop pollinators, but which are not presently sampled in large numbers on crops flowers. Whilst we found evidence that a small number of common, generalist species make a disproportionate contribution to flower visits, many more species were identified as potential pollinators, including rare and specialist species. Furthermore, we found evidence of substantial variation in the bee communities of different crops. Establishing a national list of crop pollinators is important for practitioners and policy makers, allowing targeted management approaches for improved ecosystem services, conservation and species monitoring. Data can be used to make recommendations about how pollinator diversity could be promoted in agricultural landscapes. Our results suggest agri-environment schemes need to support a higher diversity of species than at present, notably of solitary bees. Management would also benefit from targeting specific species to enhance crop pollination services to particular crops. Whilst our study is focused upon Great Britain, our methodology can easily be applied to other countries, crops and groups of pollinating insects.LH was funded by NERC QMEE CDT. EJB was funded by a BBSRC Ph.D. studentship under grant BB/F016581/1. LB was was supported by the Scholarship Program of the German Federal Environmental Foundation (Deutsche Bundesstiftung Umwelt, DBU, AZ 20014/302). AJC was funded by the BBSRC and Syngenta UK as part of a case award Ph.D. (grant no. 1518739). AE was funded by the Swiss National Science Foundation (grant number 405940-115642). DG and A-MK were funded by grant PCIN2014-145-C02-02 (MinECo; EcoFruit project BiodivERsA-FACCE2014-74). MG was supported by Establishing a UK Pollinator Monitoring and Research Partnership (PMRP) a collaborative project funded by Defra, the Welsh and Scottish Governments, JNCC and project partners’. GAdG was funded via research projects BO-11-011.01-051 and BO-43-011.06-007, commissioned by the Dutch Ministry of Agriculture, Nature and Food Quality. DK was funded by the Dutch Ministry of Economic Affairs (BO-11-011.01-011). AK-H was funded by the NKFIH project (FK123813), the Bolyai János Fellowship of the MTA, the ÚNKP-19-4-SZIE-3 New National Excellence Program of the Ministry for Innovation and Technology, and together with RF by the Hungarian Scientific Research Fund OTKA 101940. MM was funded by Waitrose & Partners, Fruition PO, and the University of Worcester. MM was funded by grant INIA-RTA2013-00139-C03-01 (MinECo and FEDER). BBP and RFS were funded by the UK Natural Environment Research Council as part of Wessex BESS (ref. NE/J014680/1). NJV was funded by the Walloon Region (Belgium) Direction générale opérationnelle de l’Agriculture, des Ressources naturelles et de l’Environnement (DGO3) for the "Modèle permaculturel" project on biodiversity in micro-farms, FNRS/FWO joint programme EOS — Excellence Of Science CliPS: Climate change and its impact on Pollination Services (project 30947854)". CW was funded by the Deutsche Forschungsgemeinschaft (DFG) (Project number 405945293). BW was funded by the Natural Environment Research Council (NERC) under research programme NE/N018125/1 ASSIST – Achieving Sustainable Agricultural Systems www.assist.ceh.ac.uk. TB and TO are supported by BBSRC, NERC, ESRC and the Scottish Government under the Global Food Security Programme (Grant BB/R00580X/1)

Damaged Intestinal Epithelial Integrity Linked to Microbial Translocation in Pathogenic Simian Immunodeficiency Virus Infections

The chronic phase of HIV infection is marked by pathological activation of the immune system, the extent of which better predicts disease progression than either plasma viral load or CD4+ T cell count. Recently, translocation of microbial products from the gastrointestinal tract has been proposed as an underlying cause of this immune activation, based on indirect evidence including the detection of microbial products and specific immune responses in the plasma of chronically HIV-infected humans or SIV-infected Asian macaques. We analyzed tissues from SIV-infected rhesus macaques (RMs) to provide direct in situ evidence for translocation of microbial constituents from the lumen of the intestine into the lamina propria and to draining and peripheral lymph nodes and liver, accompanied by local immune responses in affected tissues. In chronically SIV-infected RMs this translocation is associated with breakdown of the integrity of the epithelial barrier of the gastrointestinal (GI) tract and apparent inability of lamina propria macrophages to effectively phagocytose translocated microbial constituents. By contrast, in the chronic phase of SIV infection in sooty mangabeys, we found no evidence of epithelial barrier breakdown, no increased microbial translocation and no pathological immune activation. Because immune activation is characteristic of the chronic phase of progressive HIV/SIV infections, these findings suggest that increased microbial translocation from the GI tract, in excess of capacity to clear the translocated microbial constituents, helps drive pathological immune activation. Novel therapeutic approaches to inhibit microbial translocation and/or attenuate chronic immune activation in HIV-infected individuals may complement treatments aimed at direct suppression of viral replication

Sooty Mangabey Genome Sequence Provides Insight into AIDS Resistance in a Natural SIV Host

In contrast to infections with human immunodeficiency virus (HIV) in humans and simian immunodeficiency virus (SIV) in macaques, SIV infection of a natural host, sooty mangabeys (Cercocebus atys), is non-pathogenic despite high viraemia. Here we sequenced and assembled the genome of a captive sooty mangabey. We conducted genome-wide comparative analyses of transcript assemblies from C. atys and AIDS-susceptible species, such as humans and macaques, to identify candidates for host genetic factors that influence susceptibility. We identified several immune-related genes in the genome of C. atys that show substantial sequence divergence from macaques or humans. One of these sequence divergences, a C-terminal frameshift in the toll-like receptor-4 (TLR4) gene of C. atys, is associated with a blunted in vitro response to TLR-4 ligands. In addition, we found a major structural change in exons 3-4 of the immune-regulatory protein intercellular adhesion molecule 2 (ICAM-2); expression of this variant leads to reduced cell surface expression of ICAM-2. These data provide a resource for comparative genomic studies of HIV and/or SIV pathogenesis and may help to elucidate the mechanisms by which SIV-infected sooty mangabeys avoid AIDS

Effects of climate input data aggregation on modelling regional crop yields

Crop models can be sensitive to climate input data aggregation and this response may differ among models. This should be considered when applying field-scale models for assessment of climate change impacts on larger spatial scales or when coupling models across scales. In order to evaluate these effects systematically, an ensemble of ten crop models was run with climate input data on different spatial aggregations ranging from 1, 10, 25, 50 and 100 km horizontal resolution for the state of North Rhine-Westphalia, Germany. Models were minimally calibrated to typical sowing and harvest dates, and crop yields observed in the region, subsequently simulating potential, water-limited and nitrogen-limited production of winter wheat and silage maize for 1982-2011. Outputs were analysed for 19 variables (yield, evapotranspiration, soil organic carbon, etc.). In this study the sensitivity of the individual models and the model ensemble in response to input data aggregation is assessed for crop yield. Results show that the mean yield of the region calculated from climate time series of 1 km horizontal resolution changes only little when using climate input data of higher aggregation levels for most models. However, yield frequency distributions change with aggregation, resembling observed data better with increasing resolution. With few exceptions, these results apply to the two crops and three production situations (potential, water-, nitrogen-limited) and across models including the model ensemble, regardless of differences among models in simulated yield levels and spatial yield patterns. Results of this study improve the confidence of using crop models at varying scales