The comparative estimation of influence of higholeic sunflower and palm oils consumption on the fatty acids content of rat liver lipids

Aim: To determine of influence of higholeic sunflower and palm oils consumption on the fatty acids content of rat liver lipids.Materials and methods: The content of liver lipids and fatty acid levels in 2 lipid fractions were determined in 3 groups of rats: 1 – fat fee ration, 2 – ration with 15 % higholeic sunflower oil and 3 – ration with 15 % palm oil. The duration of feeding was 40 days. First lipid fraction was triglycerides (TG) + ester cholesterine (EC). Second lipid fraction was free fatty acids (FFA).Results: Increase of weight was the largest for third group, and the least for second group. The content of lipids in liver was the largest for third group, and the last for first group. The content of FFA was the largest for third group. The content of ω-6 PUSFA was the largest for third group. The content ω-3 PUSFA was the largest for second group.Conclusion: Palm oil consumption leact to increase sueight, the content of lipids and ω-6 PUSFA or decrease the content of ω-3 PUSFA on liver. Higholeic acid sunflower oil increase the content of ω-3 PUSFA

Hypolipidemic action of oral applications of edible fats

Levitsky A. P., Dvulit I. P., Khodakov I. V. Hypolipidemic action of oral applications of edible fats. Journal of Education, Health and Sport. 2018;8(11):600-605. eISNN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.2535711 http://ojs.ukw.edu.pl/index.php/johs/article/view/6454 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part b item 1223 (26/01/2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author(s) 2018; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 19.11.2018. Revised: 22.11.2018. Accepted: 30.11.2018. UDK 616.153:588.152:616.633:612.31 HYPOLIPIDEMIC ACTION OF ORAL APPLICATIONS OF EDIBLE FATS A. P. Levitsky 1, I. P. Dvulit2, I. V. Khodakov1 1SE «The Institute of Stomatology and Maxillofacial surgery of the National academy of medical science of Ukraine» (Odessa) 2Lviv National Medical University [email protected] Abstract Background. To determine the effect of oral fatty applications on the content of triglycerides in the serum and liver of rats. Methods. As edible fats, ordinary sunflower oil, high-oleic sunflower oil “Olivka” and butter, as well as the same oils after heat treatment, were used. Application of oils (0.5 g) on the oral mucosa was carried out once a day for 3 days. After euthanasia on the 4th day, the content of triglycerides (TG) in the serum and in the liver was determined. The content of malondialdehyde (MDA) was also determined in the liver. The ratio of the level of TG in serum and in the liver was calculated fat-incretory function of the liver. Results. Oral fatty applications cause a decrease in serum TG and increase them in the liver. In the liver, the content of MDA and the fat-incretory function decreases. Conclusion. Oral fatty applications cause hypotriglyceridemia, reduce the level of peroxidation in the liver and cause hepatosteatosis. Keywords: fat diet, oral cavity, liver, serum

Hypolipidemic action of oral applications of edible fats

Background. To determine the effect of oral fatty applications on the content of triglycerides in the serum and liver of rats. Methods. As edible fats, ordinary sunflower oil, high-oleic sunflower oil “Olivka” and butter, as well as the same oils after heat treatment, were used. Application of oils (0.5 g) on the oral mucosa was carried out once a day for 3 days. After euthanasia on the 4th day, the content of triglycerides (TG) in the serum and in the liver was determined. The content of malondialdehyde (MDA) was also determined in the liver. The ratio of the level of TG in serum and in the liver was calculated fat-incretory function of the liver. Results. Oral fatty applications cause a decrease in serum TG and increase them in the liver. In the liver, the content of MDA and the fat-incretory function decreases. Conclusion. Oral fatty applications cause hypotriglyceridemia, reduce the level of peroxidation in the liver and cause hepatosteatosis

Influence of oral fatty applications on biochemical indicators of inflammation and dysbiosis in the tissues of the rat mouth

Background. The negative impact on the body of high-fat nutrition largely depends on the fatty acid composition of edible fats and the presence of peroxidation products in them, which are formed during heat treatment. Despite the considerable number of studies on this issue, it remains unclear the nature of the influence of different dietary fats on the condition of the tissues of the oral cavity. The purpose of this study was to clarify this issue Methods. Ordinary (high-linoleic) sunflower oil, high-oleic sunflower oil and butter in the native state and after heat treatment were used. The experiments were carried out on 35 white rats with an average weight of 252±15 g, who were given oral applications of the above fats at a dose of 0.5 ml per rat for 3 days. After rat killing on the 4th day, the content of malondialdehyde (MDA), the activity of elastase, urease, lysozyme and catalase were determined in the cheek mucosa homogenate and in the gums. The degree of dysbiosis was calculated by the ratio of the relative activities of urease and lysozyme. The antioxidant-prooxidant API index was calculated from the ratio of catalase activity and MDA content Results. Oral applications of native and heat-treated oils cause a decrease in the MDA content in both tissues with the exception of heat-treated butter, applications of which do not reduce the MDA level. Applications of heat-treated oils increase the activity of elastase in the gums, and heat-treated sunflower oil in the cheek mucosa. Applications of native and heat-treated oils reduce the activity of catalase in the cheek and gums. The API index increases in the gums under the action of native oils and decreases under the action of thermally processed oils. Applications of butter (native and heat-treated) significantly reduce the activity of urease in the cheek mucosa. Sunflower and high-oleic sunflower oils tend to increase the activity of urease, the least pronounced for the latter. All oils except high-oleic sunflower, reduce the activity of lysozyme in both tissues and all oils increase the degree of dysbiosis (except butter in the cheek mucosa). The content of diene conjugates after heat treatment increased the most in butter, and the least in high-oleic sunflower oil. Conclusion. Oral applications of fats cause a decrease in the formation of active forms of oxygen in the tissues of the oral cavity, which causes the development of dysbiosis and inflammatory processes

Small Molecule Activation by Uranium Tris(aryloxides): Experimental and Computational Studies of Binding of N-2, Coupling of CO, and Deoxygenation Insertion of CO2 under Ambient Conditions

Previously unanticipated dinitrogen activation is exhibited by the well-known uranium tris(aryloxide) U(ODtbp)(3), U(OC6H3-Bu-2(t)-2,6)(3), and the tri-tert-butyl analogue U(OTtbp)(3), U(OC6H2-Bu-3(t)-2,4,6)(3), in the form of bridging, side-on dinitrogen complexes [U(OAr)(3)](2)(mu-eta(2):eta(2)-N-2), for which the tri-tert-butyl N-2 complex is the most robust U-2(N-2) complex isolated to date. Attempted reduction of the tris(aryloxide) complex under N-2 gave only the potassium salt of the uranium(III) tetra(aryloxide) anion, K[U(OAr)(4)], as a result of ligand redistribution. The solid-state structure is a polymeric chain formed by each potassium cation bridging two arenes of adjacent anions in an eta(6) fashion. The same uranium tris(aryloxides) were also found to couple carbon monoxide under ambient conditions to give exclusively the ynediolate [OCCO](2-) dianion in [U(OAr)(3)](2)(mu-eta(1):eta(1)-C2O2), in direct analogy with the reductive coupling recently shown to afford [U{N(SiMe3)(2)}(3)](2)(mu-eta(1):eta(1)-C2O2). The related U-III complexes U{N(SiPhMe2)(2)}(3) and U{CH(SiMe3)(2)}(3) however do not show CO coupling chemistry in our hands. Of the aryloxide complexes, only the U(OC6H2-Bu-3(t)-2,4,6)(3) reacts with CO2 to give an insertion product containing bridging oxo and aryl carbonate moieties, U-2(OTtbp)(4)(mu-O)(mu-eta(1):eta(1)-O2COC6H2-Bu-3(t)-2,4,6)(2), which has been structurally characterized. The presence of coordinated N-2 in [U(OTtbp)(3)](2)(N-2) prevents the occurrence of any reaction with CO2, underscoring the remarkable stability of the N-2 complex. The di-tert-butyl aryloxide does not insert CO2, and only U(ODtbp)(4) was isolated. The silylamide also reacts with carbon dioxide to afford U(OSiMe3)(4) as the only uranium-containing material. GGA and hybrid DFT calculations, in conjunction with topological analysis of the electron density, suggest that the U-N-2 bond is strongly polar, and that the only covalent U -> N-2 interaction is pi backbonding, leading to a formal (U-IV)(2)(N-2)(2-) description of the electronic structure. The N-N stretching wavenumber is preferred as a metric of N-2 reduction to the N-N bond length, as there is excellent agreement between theory and experiment for the former but poorer agreement for the latter due to X-ray crystallographic underestimation of r(N-N). Possible intermediates on the CO coupling pathway to [U(OAr)(3)](2)(mu-C2O2) are identified, and potential energy surface scans indicate that the ynediolate fragment is more weakly bound than the ancillary ligands, which may have implications in the development of low-temperature and pressure catalytic CO chemistry

LONG-TERM RESULTS OF SURGICAL TREATMENT OF DIVERTICULAR DISEASE OF COLON

The work evaluated the long-term treatment results of 103 patients with complicated diverticular disease, who underwent the elective surgery in the volume of left hemicolectomy. The first group included 53 patients with recurrent diverticula. The second group consisted of 50 patients, who underwent the operation on diverticula of sigmoid colon with formation of sigmostoma on the first stage. The number of patients with functional gastro-intestinal and psyco-emotional disorders was more significant in the first group compared with the second group and it was associated with presence of these disorders before the operation. There was noted the reliable decrease of quality of life rates according to questionnaire scales MOS-SF 36 in the first group. The average indices of scales had some matching to the rates of health people in the patients of the second group. The authors came to conclusion, that indications for elective surgery should be determined very carefully in case of recurrent diverticula