Background. To determine the effect of oral fatty applications on the content of triglycerides in the serum and  liver of rats.

Methods. As edible fats, ordinary sunflower oil, high-oleic sunflower oil “Olivka” and butter, as well as the same oils after heat treatment, were used. Application of oils (0.5 g) on the oral mucosa was carried out once a day for 3 days. After euthanasia on the 4th day, the content of triglycerides (TG) in the serum and in the liver was determined. The content of malondialdehyde (MDA) was also determined in the liver. The ratio of the level of TG in serum and in the liver was calculated fat-incretory function of the liver.

Results. Oral fatty applications cause a decrease in serum TG and increase them in the liver. In the liver, the content of MDA and the fat-incretory function decreases. 

Conclusion. Oral fatty applications cause hypotriglyceridemia, reduce the level of peroxidation in the liver and cause hepatosteatosis

A. P. Levitsky

I. P. Dvulit

I. V. Khodakov

Journal of Education Health and Sport

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 600 Levitsky A. P., Dvulit I. P., Khodakov I. V. Hypolipidemic action of oral applications of edible fats. Journal of Education, Health and Sport. 2018;8(11):600-605. eISNN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.2535711  http://ojs.ukw.edu.pl/index.php/johs/article/view/6454      The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part b item 1223 (26/01/2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author(s) 2018; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 19.11.2018. Revised: 22.11.2018. Accepted: 30.11.2018.   UDK 616.153:588.152:616.633:612.31  HYPOLIPIDEMIC ACTION OF ORAL APPLICATIONS OF EDIBLE FATS  A. P. Levitsky 1, I. P. Dvulit2, I. V. Khodakov1  1SE «The Institute of Stomatology and Maxillofacial surgery of the National academy of medical science of Ukraine» (Odessa) 2Lviv National Medical University flavan.ua@gmail.com  Abstract Background. To determine the effect of oral fatty applications on the content of triglycerides in the serum and  liver of rats. Methods. As edible fats, ordinary sunflower oil, high-oleic sunflower oil “Olivka” and butter, as well as the same oils after heat treatment, were used. Application of oils (0.5 g) on the oral mucosa was carried out once a day for 3 days. After euthanasia on the 4th day, the content of triglycerides (TG) in the serum and in the liver was determined. The content of malondialdehyde (MDA) was also determined in the liver. The ratio of the level of TG in serum and in the liver was calculated fat-incretory function of the liver. Results. Oral fatty applications cause a decrease in serum TG and increase them in the liver. In the liver, the content of MDA and the fat-incretory function decreases.  Conclusion. Oral fatty applications cause hypotriglyceridemia, reduce the level of  601 peroxidation in the liver and cause hepatosteatosis. Keywords: fat diet, oral cavity, liver, serum.   INTRODUCTION In our previous work [1], it was shown that fatty applications of edible fats on the oral mucosa of the rats (cheek, gums) cause a decrease in the level of lipid peroxidation (LPO) processes in these tissues due to activation of antioxidant systems. The purpose of this work was to determine the effect of oral applications on the content of triglycerides in the serum and liver of rats. MATERIAL AND RESEARCH METHODS The following edible fats were used in the work: sunflower high-linoleic oil, refined, high-olein sunflower oil “Olive” [2] and butter (fat content 82%). A more detailed description of these fats is presented in our previous work [1]. Heat treatment of oils was carried out by heating at 125 ° C for 60 minutes in the presence of 1.5% hydrogen peroxide (30%). The experiments were carried out on 35 white Wistar rats (males, 13 months, average live weight 232 ± 15 g), divided into 7 equal groups: 1st – control, 2nd – received applications to the oral mucosa 0.5 ml of native sunflower oil, the 3rd group – 0.5 ml of heat-treated sunflower oil, the 4th group - received oral applications of high-oleic sunflower oil (0.5 ml per rat), the 5th group - applications of 0.5 ml of the heat-treated high olein sunflower oil, 6th - 0.5 ml of butter and 7th - 0.5 ml of heat-treated butter. The duration of the experiment was 3 days and on the 4th day, the rats were killed under thiopental anesthesia (20 mg / kg) by total bleeding from the heart. Serum was obtained and the liver part was excised for biochemical research. The content of triglycerides (TG) in the liver homogenate was determined by an enzymatic method [3] and the content of malonic dialdehyde (MDA) using the reaction with thiobarbituric acid [4]. In the serum was determined by the content of TG and MDA. The ratio of the content of TG in the blood serum and in the liver was calculated fat-incretory function (FIF) of the liver by the formula:  602 100TGliverTGserumFIF  RESULTS AND DISCUSSION In fig. 1 presents the results of determining the content of MDA in the liver, which shows (with one exception, perhaps an artifact) a significant decrease in the content of MDA, similar to what we observed in the gums of rats [1]. 010203040506070C SO HOSO BMDA, mmol/kgnative fats heat treated fats***** Fig. 1. Influence of oral application of edible fats on the content of MDA in rat liver (C – control, SO – sunflower oil, HOSO – high oleic sunflower oil, B – butter)  In fig. 2 presents the results of the determination of the TG content in the liver of rats treated with oral fatty applications. It is seen that in almost all cases there is an increase in the fat content in the liver after oral application of fat. On the contrary, in the serum of rats after oral fat application, the TG content is significant decrease, especially after the application of heat-treated fats (Fig. 3). Since the serum TG is represented in the composition of very low density lipoproteins (VLDL), which are produced exclusively by the liver [5], we propose to evaluate the liver fat- incretory function (FIF) by the ratio of serum TG and liver TG. The results of this assessment are presented in fig. 4, from which it follows that oral fatty applications significantly reduce this indicator, indicating the suppression of this liver function.  603 048121620C SO HOSO BTG, mmol/kgnative fats heat treated fats * * *** Fig. 2. The effect of oral application of edible fats on the TG content in the liver of rats (C – control, SO – sunflower oil, HOSO – high oleic sunflower oil, B – butter)  00,120,240,360,480,60,72C SO HOSO BTG, mmol/lnative fats heat treated fats** * Fig. 3. The effect of oral applications of edible fats on the TG content in the serum of rats  (C – control, SO – sunflower oil, HOSO – high oleic sunflower oil, B – butter)   604 02468C SO HOSO BTGs/TGl х 100native fats heat treated fats * * * * * * Fig. 4. Influence of oral application of edible fats on the fat- incretory function of the liver  (C – control, SO – sunflower oil, HOSO – high oleic sunflower oil, B – butter)  One can only assume that the observed hypolipidemia and a decrease in the fat- incretory function of the liver are the result of a reflex reaction to the fatty application to the oral mucosa [5]. Further studies should shed light on the possible physiological mechanism of this reflex. CONCLUSIONS 1. Oral applications of edible fats cause hypotriglyceridemia, steatosis and a decrease in the level of LPO in the liver in rats. 2. Oral applications of fats, especially those thermally treated, significantly inhibit the fat- incretory function of the liver. 3. The lipid-lowering effect of oral fatty applications may have a reflex character.  REFERENCES  1. Markov AV, Labush IuZ, Khodakov IV, Levitsky AP, Varava GN. Influence of oral fatty applications on biochemical indicators of inflammation and dysbiosis in the tissues of the rat mouth. Journal of Education, Health and Sport. 2018; 8(10): 392-404. 2. Levitsky AP. Olivka: the unique sunflower oil, the analogue to olive oil. Odessa, KP of the OSG, 2016: 27. (in Russian)  605 3. The instruction to the set of reagents for the determination of triglycerides in blood serum and plasma with enzymatic colorimetric method / ТU U 24.4-24607793-020-2003. (in Ukrainian) 4. Levitsky AP, Makarenko OA, Khodakov ІV. Methods to investigate fats and oils. Odessa, KP of the OSG, 2015: 32. (in Russian) 5. Song HJ, Bell GA. Human perception of fat: 4th Congr. FAOPS, 2nd Congr. FAONS, 66th Meet. APAS and Annu Meet PSNZ, Brisbane, Sept. 27th-Oct. 1, 1998. Austral. Physiol. and Pharmacol. Soc. 1998; 29(2): 242. 

Hypolipidemic action of oral applications of edible fats

Levitsky A. P., Dvulit I. P., Khodakov I. V. Hypolipidemic action of oral applications of edible fats. Journal of Education, Health and Sport. 2018;8(11):600-605. eISNN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.2535711

http://ojs.ukw.edu.pl/index.php/johs/article/view/6454

 

 

 

 

The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part b item 1223 (26/01/2017).

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

© The Author(s) 2018;

This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland

Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,

provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license

(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.

This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial

use, distribution and reproduction in any medium, provided the work is properly cited.

The authors declare that there is no conflict of interests regarding the publication of this paper.

Received: 19.11.2018. Revised: 22.11.2018. Accepted: 30.11.2018.

 

 

UDK 616.153:588.152:616.633:612.31

 

HYPOLIPIDEMIC ACTION OF ORAL APPLICATIONS OF EDIBLE FATS

 

A. P. Levitsky 1, I. P. Dvulit2, I. V. Khodakov1

 

1SE «The Institute of Stomatology and Maxillofacial surgery of the National academy of medical science of Ukraine» (Odessa)

2Lviv National Medical University

flavan.ua@gmail.com

 

Abstract

Background. To determine the effect of oral fatty applications on the content of triglycerides in the serum and  liver of rats.

Methods. As edible fats, ordinary sunflower oil, high-oleic sunflower oil “Olivka” and butter, as well as the same oils after heat treatment, were used. Application of oils (0.5 g) on the oral mucosa was carried out once a day for 3 days. After euthanasia on the 4th day, the content of triglycerides (TG) in the serum and in the liver was determined. The content of malondialdehyde (MDA) was also determined in the liver. The ratio of the level of TG in serum and in the liver was calculated fat-incretory function of the liver.

Results. Oral fatty applications cause a decrease in serum TG and increase them in the liver. In the liver, the content of MDA and the fat-incretory function decreases.

Conclusion. Oral fatty applications cause hypotriglyceridemia, reduce the level of peroxidation in the liver and cause hepatosteatosis.

Keywords: fat diet, oral cavity, liver, serum

Levitsky, A. P.

Dvulit, I. P.

Khodakov, I. V.

ZENODO

English

NEUROSURGERY ENTHUSIASTIC WOMEN SOCIETY

Background. To determine the effect of oral fatty applications on the content of triglycerides in the serum and  liver of rats.
Methods. As edible fats, ordinary sunflower oil, high-oleic sunflower oil “Olivka” and butter, as well as the same oils after heat treatment, were used. Application of oils (0.5 g) on the oral mucosa was carried out once a day for 3 days. After euthanasia on the 4th day, the content of triglycerides (TG) in the serum and in the liver was determined. The content of malondialdehyde (MDA) was also determined in the liver. The ratio of the level of TG in serum and in the liver was calculated fat-incretory function of the liver.
Results. Oral fatty applications cause a decrease in serum TG and increase them in the liver. In the liver, the content of MDA and the fat-incretory function decreases. 
Conclusion. Oral fatty applications cause hypotriglyceridemia, reduce the level of peroxidation in the liver and cause hepatosteatosis

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Hypolipidemic action of oral applications of edible fats

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