Development of hyper osmotic resistant CHO host cells

We have developed a cell culture platform for monoclonal antibody (IgG) production by Chinese Hamster Ovary (CHO) cells. The platform feed used the continuous feeding method. This platform can maintain high cell density and produce high antibody titer. However because operation of continuous feed is complex, contract manufacturing organization (CMO) that can perform continuous feed is limited. Therefore, we tried to change the feeding method from continuous feed to bolus feed. However the previous studies showed that the rapid changes of osmolality by bolus feed and the hyper osmolality repressed the cell culture growth and the final titer. In this study, we developed hyper osmotic resistant CHO-S host cell A (resistant to 450mOsm). To establish osmotic resistant CHO-S host cells, original CHO-S cells were passaged on a hyper osmotic basal media with repetition for about 100 days. We demonstrated that there were obviously differences in the cell growth under osmotic pressure of iso- (328 mOsm) and hyper- (450 mOsm) osmolality between the two host cells. Metabolic analysis of cell culture supernatant on CHO-S host cell A and CHO-S host cells with/ without osmotic stress performed. Compared to original CHO-S host cells, the osmotic resistant CHO-S host cell A has a greater capacity to generate osmolytes (sorbitol and erthritol) and decreased level of oxidized glutathione (GSSG), which suggests the osmotic resistant CHO-S host cells A handles osmotic stress better. Moreover, the characteristic of osmotic resistant on hyper osmotic resistant CHO-S host cell A was maintained even after 7 passages on a basal medium (330 mOsm). We will establish hyper osmotic resistant antibody production CHO cell line by using the CHO-S host cell A

Leading, coaching, & mentoring: a study of coach-athlete relationships as associated factors in performance

This research focused on global leadership and change and competitive athletics. Servant leadership, including the five servant leadership variables; altruistic calling, emotional healing, wisdom, persuasive mapping, and organizational stewardship, as well coach athlete relationships, including the three coach-athlete relationship variables; closeness, commitment, and complementarity, were used to analyze athlete perceptions. This study then found correlations of the associated factors to outcome, at team and individual levels. Findings from this study include recognizing the commitment of the head coach as a factor in team mins; individual athlete perception of closeness as related to games started; the emotional healing aspects from the head coach to overall team wins; wisdom of the head coach to how many games the individual athlete played; and the differences between each teams as related to individual or team outcome. Conclusions focused on how a head coach values the individual as well as the athlete; how a head coach can impact individual athlete success and be relied upon in times of need; and how high functioning teams strive for perfection across genders and various sports. This study also highlights several recommendations focused on policy and practice within competitive athletics and global transfer

Multiple N-cadherin enhancers identified by systematic functional screening indicate its Group B1 SOX-dependent regulation in neural and placodal development

AbstractNeural plate and sensory placodes share the expression of N-cadherin and Group B1 Sox genes, represented by Sox2. A 219-kb region of the chicken genome centered by the N-cadherin gene was scanned for neural and placodal enhancers. Random subfragments of 4.5 kb average length were prepared and inserted into tkEGFP reporter vector to construct a library with threefold coverage of the region. Each clone was then transfected into N-cadherin-positive (lens, retina and forebrain) or -negative embryonic cells, or electroporated into early chicken embryos to examine enhancer activity. Enhancers 1–4 active in the CNS/placode derivatives and non-specific Enhancer 5 were identified by transfection, while electroporation of early embryos confirmed enhancers 2–4 as having activity in the early CNS and/or sensory placodes but with unique spatiotemporal specificities. Enhancers 2–4 are dependent on SOX-binding sites, and misexpression of Group B1 Sox genes in the head ectoderm caused ectopic development of placodes expressing N-cadherin, indicating the involvement of Group B1 Sox functions in N-cadherin regulation. Enhancers 1, 2 and 4 correspond to sequence blocks conserved between the chicken and mammalian genomes, but enhancers 3 and 5 are unique to the chicken

機能性ナノ多孔体を用いた無機有機複合材料の合成

九州工業大学博士学位論文 学位記番号:工博甲第424号 学位授与年月日:平成28年9月23日1. General Introduction||2. Hydrogels containing Prussian blue nanoparticles for facile removal of Cs ions||3. Hydrogels containing mesoporous silica particles for control behavior of guest molecules adsorption/desorption||4. Silicone rubbers containing mesoporous silica particles for improvement thermal property and strength||5. Gereral Conclusio

In-orbit Demonstration of Reaction Control System for Orbital Altitude Change of Micro-Satellite ALE-2

This research presents the results of an in-orbit test of the orbital altitude control for a micro-satellite equipped with the first space-demonstrated high-density small cold gas jet thruster. In the field of micro-satellites, the application of thrusters to practical missions has not yet progressed due to their high cost, mechanical and electrical incompatibility with the satellite bus system, and increased operational risks. By contrast, the demand for orbit control functions has been increasing in recent years with the expansion of micro-satellite applications. The76kg satellite ALE-2 , which was jointly developed by Tohoku University and ALE Co., Ltd., has the world\u27s first challenging mission to artificially generate shooting stars by ejecting small substances (meteor source) from the ejection device fixed on the satellite body. To avoid collision of the ejected meteor source with other flying objects, the mission must be performed in a sun-synchronous orbit at an altitude of less than 400 km, which is lower than that of the International Space Station. However, it is required to maintain the mission orbit autonomously because the orbit decay is large due to the effect of atmospheric drag. In addition, to release the meteor source at an arbitrary orbital position, it is essential to manipulate the ground track by raising and lowering the orbital altitude. Therefore, ALE-2 needs to control the orbit altitude actively and with arbitrary amount of change. In this study, the reaction control system (RCS), which satisfies the orbit change capability, mission requirements, and compatibility with the satellite bus system, is installed on ALE-2 to perform space demonstrations of orbit control and to evaluate the operational performance of the thruster. ALE-2 will be the first to be equipped with a cold gas jet thruster developed by Patched conics, LLC. It is estimated that the thruster is capable of changing altitude more than 1 km by continuous drive for one orbital period. Using this RCS, the following three criteria were set as the evaluation criteria: (Minimum) the orbit altitude can be actively changed by the thruster, (Full) the orbit altitude can be controlled by an arbitrary amount of operation and can be increased more than 1 km per orbit, and (Extra) the mission orbit can be transferred according to the meteor source release plan. ALE-2 was launched on December 6, 2019, and the in-orbit test of the RCS started four months later. Although the RCS was not able to achieve its initial orbit change capability due to an anomaly in the power supply system, various kinds of tests were conducted under conditions that allowed continuous thruster operation. It was confirmed that the orbit altitude was increased by 0.4 km per orbit. In addition, the fault detection, isolation and recovery (FDIR)function was effectively performed against any kinds of anomalies of RCS during in-orbit operation. Therefore, a sustained orbital altitude of 400 km was expected to be achievable using the onboard RCS

Brand Love and Purchase Intention: Does a Sports Team Name Matter?

This study aims to explore the relationship between the construct of brand love, the strength of fan allegiance to a sport team brand name, fan past purchase behavior, and future purchase intention should a team change its controversial name. The overall research question that bounds this study is: How does consumer brand love and a change to a sport team name impact fan purchase behavior