Adopce a její význam v prevenci psychické deprivace

Sputum basophil gating strategy and change in marker expression. Representative sputum dot plots showing basophil gating strategy used for flow cytometry analysis including: (A) Side Scatter (SSC) vs. Forward Side Scatter (FSC), (B) SSC vs. CD45 Alexa Fluor 700, (C) SSC vs. HLA-DR APCH7 and (D) SSC vs. IL-3Rι PECy7. Panels E-G are representative dot plots of the change in the percentage of basophils expressing IL-17RB, ST2, and intracellular IL-25 at pre-allergen challenge (blue), 7 h post-allergen (green) and 24 h post-allergen challenge (orange), compared to the isotype control (red). (TIFF 10198 kb

Additional file 6: Figure S6. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma

Basophil activation marker dose response to IL-25. Isolated peripheral blood basophils from 8 allergic subjects were suspended in RPMI-C and cultured for 18 h with PBS (negative control, open bars) or increasing concentrations of IL-25 (0.1, 1, 10, 100 ng/mL) (black bars), were assessed for expression of (A) CD203c, (B) IL-3Rα, (C) CCR3, (D) intracellular IL-13, (E) intracellular IL-4, (F) intracellular IL-25, (G) IL-17RB, (H) ST2, and (I) TSLPR. Data expressed as mean ± SEM. *Significantly different from PBS negative control; P < 0.05 (N = 8). (TIFF 2740 kb

Additional file 5: Figure S5. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma

Basophil activation marker dose response to IL-33. Isolated peripheral blood basophils from 8 allergic subjects were suspended in RPMI-C and cultured for 18 h with PBS (negative control, open bars) or increasing concentrations of IL-33 (0.1, 1, 10, 100 ng/mL) (black bars), were assessed for expression of (A) CD203c, (B) IL-3Rα, (C) CCR3, (D) intracellular IL-13, (E) intracellular IL-4, (F) intracellular IL-25, (G) IL-17RB, (H) ST2, and (I) TSLPR. Data expressed as mean ± SEM. *Significantly different from PBS negative control; P < 0.05 (N = 8). (TIFF 2756 kb

Additional file 1: Figure S1. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma

Bone marrow basophil gating strategy and change in marker expression. Representative bone marrow dot plots showing basophil gating strategy used for flow cytometry analysis including: (A) Side Scatter (SSC) vs. Forward Side Scatter (FSC), (B) SSC vs. CD45 Alexa Fluor 700, (C) SSC vs. HLA-DR APCH7 and (D) SSC vs. IL-3Rι PECy7. Panels E-G are representative dot plots of the change in the percentage of basophils expressing IL-17RB, ST2, and intracellular IL-25 at pre-allergen challenge (blue) and 24 h post-allergen challenge (green), compared to the isotype control (red). (TIFF 10587 kb

MOESM2 of Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma

Additional file 2: Figure S2. Histological analysis of lungs. Hematoxylin-eosin staining of the C57BL/6 in wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control)