Systemic Design Method for Co-creation of 3D Printing Service

[EN] Background: As one of the objectives of Design for Additive Manufacture, the customized geometry promotes 3D printing to increasingly realize product customization in the service market. Defined as a business strategy which focuses on customer experience and interaction, co-creation is expected to obtain a fast-growing market volume. Recently, some co-creation of 3D printing service (3DPS) has been released to realize value creation. Despite of its rapid growth, there are rare researches on this field, especially those about its design method. Aim: To define a systematic design method for developing the co-creation of 3DPS. Method: Firstly, this research distinguished ambiguous type and definite type of 3DPS cocreation. The latter was taken as the current research object, because it presents the services scope more clearly. Furthermore, in order to solve the problem about the research, that is, what the essential components constructing the 3DPS co-creation are, evidence needed to be collected based on observation of the mentioned cases. Therefore, holistic multiple-case study of 3DPS co-creation samples was designed and conducted, as it was herein applied as the research method. This research is divided into three sections. The first section presents the preparation for data collection, including case selection and the formulation of evidence collection. The second section analyzes the collected evidences. Based on the evidence analysis, the third section concludes the knowledge of 3DPS cocreation. In order to collect adequate evidences, a pair of models was applied to build a framework. The first one is the Den Hertog's service innovation model which presents four dimensions including new service concept, new client interface, new service delivery system, and technological options. The other model refers to the building blocks of interactions for value co-creation: dialogue, access, risk-benefits, and transparency. It presents the components in basis construction, which are necessary for the interactions between a consumer and a service provider. Finding: the system of 3DPS co-creation is composed by three dialogues including related accesses and interfaces, and the to-be-3D printed outcome. The three accesses provide customers with the entrances of knowing service concept, co-creating geometry, and accepting service delivery. The interfaces bring corresponding dialogues between accesses and customer to reach each process goal. The outcome of co-creation refers to the 3D printed artifact or 3D digital model. Conclusion: This research proposes a four-step systemic design method for co-creation of 3DPS. Firstly, the dialogue with the interface of service concept introduction and the access to know it is constructed. Secondly, the dialogue based on the interface of cocreation with design variables, and the access of co-creating geometry is built. WebGL supports its 3D graphics. Thirdly, the interface of purchasing or downloading, and the access of accepting service delivery compose the dialogue of this step. Fourthly, the customized artifact shall be treated by 3D printing and then delivered to customers; or a 3D digital model gets ready for downloading.Zhou, D.; Jiang, J.; Zou, Y. (2016). Systemic Design Method for Co-creation of 3D Printing Service. En Systems&design:beyond processes and thinking. Editorial Universitat Politècnica de València. 883-900. https://doi.org/10.4995/IFDP.2015.3144OCS88390

A Research into the Design Strategies for Public Seating in a Windy Environment

Abstract-Public seating, as one of the most important urban infrastructures, has been widely installed throughout cities. Little prior research has paid attention to the influence of the natural environment on public seating services and citizens. Based on quantitative data collection statistics from public seating occupancy in a specific windy environment, and user-centred behavioural observations and interviews, this research generated results in the form of charts, texts and images. Based on the findings of this research, design strategies for public seating in seaside windy environment can be deduced, aimed at creating high levels of comfort and satisfaction for the public

LBH589 Inhibits proliferation and metastasis of hepatocellular carcinoma via inhibition of gankyrin/stat3/akt pathway

Background: Gankyrin has shown to be overexpressed in human liver cancers and plays a complex role in hepatocarcinogenesis. Panobinostat (LBH589), a new hydroxamic acid-derived histone deacetylase inhibitor has shown promising anticancer effects recently. Here, we investigated the potential of LBH589 as a form of treatment for hepatocellular carcinoma (HCC). Methods: Gankyrin plasmid was transfected into HCC cells, and the cells were selected for more than 4 weeks by incubation with G418 for overexpression clones. The therapeutic effects of LBH589 were evaluated in vitro and in vivo. Cell proliferation, apoptosis, cell cycle, invasive potential, and epithelial-mesenchy-mal transition (EMT) were examined. Results: LBH589 significantly inhibited HCC growth and metastasis in vitro and in vivo. Western blotting analysis indicated that LBH589 could decrease the expression of gankyrin and subsequently reduced serine-phosphorylated Akt and tyrosine-phosphorylated STAT3 expression although the total Akt and STAT3 were unaffected. LBH589 inhibited metastasis in vitro via down-regulation of N-cadherin, vimentin, TWIST1, VEGF and up-regulation of E-cadherin. LBH589 also induced apoptosis and G1 phase arrest in HCC cell lines. Ectopic expression of gankyrin attenuated the effects of LBH589, which indicates that gankyrin might play an important role in LBH589 mediated anticancer effects. Lastly, in vivo study indicated that LBH589 inhibited tumor growth and metastasis, without discernable adverse effects comparing to control group, with abrogating gankyrin/STAT3/Akt pathway. Conclusions: Our results suggested that LBH589 could inhibit HCC growth and metastasis through down-regulating gankyrin/STAT3/Akt pathway. LBH589 may present itself as a novel therapeutic strategy for HCC

Fabrication, microstructures, and optical properties of Yb:Lu2O3 laser ceramics from co-precipitated nano-powders

AbstractThe Yb:Lu2O3 precursor made up of spherical particles was synthesized through the co-precipitation method in the water/ethanol solvent. The 5 at% Yb:Lu2O3 powder is in the cubic phase after calcination at 1100 °C for 4 h. The powder also consists of spherical nanoparticles with the average particle and grain sizes of 96 and 49 nm, respectively. The average grain size of the pre-sintered ceramic sample is 526 nm and that of the sample by hot isostatic pressing grows to 612 nm. The 1.0 mm-thick sample has an in-line transmittance of 81.6% (theoretical value of 82.2%) at 1100 nm. The largest absorption cross-section at 976 nm is 0.96×10−20 cm2 with the emission cross-section at 1033 nm of 0.92×10−20 cm2 and the gain cross sections are calculated with the smallest population inversion parameter β of 0.059. The highest slope efficiency of 68.7% with the optical efficiency of 65.1% is obtained at 1033.3 nm in quasi-continuous wave (QCW) pumping. In the case of continuous wave (CW) pumping, the highest slope efficiency is 61.0% with the optical efficiency of 54.1%. The obtained laser performance indicates that Yb:Lu2O3 ceramics have excellent resistance to thermal load stresses, which shows great potential in high-power solid-state laser applications

First-principles atomistic Wulff constructions for an equilibrium rutile TiO2 shape modeling

Identifying the exposed surfaces of rutile TiO2 crystal is crucial for its industry application and surface engineering. In this study, the shape of the rutile TiO2 was constructed by applying equilibrium thermodynamics of TiO2 crystals via first-principles density functional theory (DFT) and Wulff principles. From the DFT calculations, the surface energies of six low-index stoichiometric facets of TiO2 are determined after the calibrations of crystal structure. And then, combined surface energy calculations and Wulff principles, a geometric model of equilibrium rutile TiO2 is built up, which is coherent with the typical morphology of fully-developed equilibrium TiO2 crystal. This study provides fundamental theoretical guidance for the surface analysis and surface modification of the rutile TiO2-based materials from experimental research to industry manufacturing. (C) 2017 Elsevier B.V. All rights reserved

Nutlin-3 overcomes arsenic trioxide resistance and tumor metastasis mediated by mutant p53 in Hepatocellular Carcinoma

Background: Arsenic trioxide has been demonstrated as an effective anti-cancer drug against leukemia and solid tumors both in vitro and in vivo. However, recent phase II trials demonstrated that single agent arsenic trioxide was poorly effective against hepatocellular carcinoma (HCC), which might be due to drug resistance. Methods: Mutation detection of p53 gene in arsenic trioxide resistant HCC cell lines was performed. The therapeutic effects of arsenic trioxide and Nutlin-3 on HCC were evaluated both in vitro and in vivo. A series of experiments including MTT, apoptosis assays, co-Immunoprecipitation, siRNA transfection, lentiviral infection, cell migration, invasion, and epithelial-mesenchy-mal transition (EMT) assays were performed to investigate the underlying mechanisms. Results: The acquisition of p53 mutation contributed to arsenic trioxide resistance and enhanced metastatic potential of HCC cells. Mutant p53 (Mutp53) silence could re-sensitize HCC resistant cells to arsenic trioxide and inhibit the metastatic activities, while mutp53 overexpression showed the opposite effects. Neither arsenic trioxide nor Nutlin-3 could exhibit obvious effects against arsenic trioxide resistant HCC cells, while combination of them showed significant effects. Nutlin-3 can not only increase the intracellular arsenicals through inhibition of p-gp but also promote the p73 activation and mutp53 degradation mediated by arsenic trioxide. In vivo experiments indicated that Nutlin-3 can potentiate the antitumor activities of arsenic trioxide in an orthotopic hepatic tumor model and inhibit the metastasis to lung. Conclusions: Acquisitions of p53 mutations contributed to the resistance of HCC to arsenic trioxide. Nutlin-3 could overcome arsenic trioxide resistance and inhibit tumor metastasis through p73 activation and promoting mutant p53 degradation mediated by arsenic trioxide

Diphenyl Difluoroketone: A Potent Chemotherapy Candidate for Human Hepatocellular Carcinoma

Diphenyl difluoroketone (EF24), a molecule having structural similarity to curcumin, was recently reported to inhibit proliferation of various cancer cells significantly. Here we try to determine the effect and mechanism of EF24 on hepatocellular carcinoma. 2 µM EF24 was found to inhibit the proliferation of PLC/PRF/5, Hep3B, HepG2, SK-HEP-1 and Huh 7 cell lines. However, even 8 µM EF24 treatment did not affect the proliferation of normal liver LO2 cells. Accordingly, 20 mg/kg/d EF24 inhibited the growth of the tumor xenografts conspicuously while causing no apparent change in liver, spleen or body weight. In addition, significant apoptosis and G2/M phase cell cycle arrest were found using flow cytometry. Besides, caspases and PARP activation and features typical of apoptosis including fragmented nuclei with condensed chromatin were also observed. Furthermore, the mechanism was targeted at the reduction of nuclear factor kappa b (NF-κB) pathway and the NF-κB–regulated gene products Bcl-2, COX-2, Cyclin B1. Our study has offered a strategy that EF24 being a therapeutic agent for hepatocellular carcinoma

Ultrasonic-assisted synthesis of porous S-doped carbon nitride ribbons for photocatalytic reduction of CO2

A series of porous S-doped carbon nitride ribbons (PSCN) were prepared by one-pot hydrothermal and sonochemical synthesis techniques. The morphologies and nanostructures of the catalysts were characterized by SEM, XRD and IR, which confirmed the pristine graphitic structures of carbon nitrides retained in the products. Due to sonication treatment, PSCN has porous structures in the thin ribbon and larger specific surface areas (PSCN 43.5 m2/g, SCN 26.6 m2/g and GCN 6.5 m2/g). XPS and elemental mappings verified that sulfur atoms were successfully introduced into the carbon nitride framework. Diffuse reflectance spectroscopy (DRS) results showed S-doping in the carbon nitride reduced the bandgap energy and enhanced their capability of the utilization of visible light, which contributed to higher photo-generated current. Photoluminescence (PL) analysis indicates the recombination of photogenerated carriers was suppressed in PSCN. Moreover, the photocatalytic performance showed that S-doping and porous and thin ribbon nanostructures may effectively boost the CO2 reduction rate (to as much as 5.8 times of GCN) when illuminated by visible light (>420 nm) without the need of sacrificial materials. The preliminary mechanisms of the formation of PSCN and its applications in photocatalytic CO2 reduction are proposed. It highlights the potential of the current technique to produce effective, nonmetal-doped carbon nitride photocatalysts

Factors Influencing the Aged in the Use of Mobile Healthcare Applications: An Empirical Study in China

Mobile healthcare applications are of significant potential value in the development of the aged-care industry due to their great convenience, high efficiency, and low cost. Since the cognition and utilization rates of mobile healthcare applications for the elderly are still low, this study explored the factors that affect the elderly’s adoption of mobile healthcare applications. This study conducted a questionnaire survey on the elderly in China and received 365 valuable responses. This study combined the technology acceptance model, protection motivation theory, and perceived risk theory to build a research model of factors affecting the use of mobile healthcare applications by the elderly. The data were analyzed using a structural equation model. The results were as follows: according to the empirical research, (1) perceived usefulness and perceived ease of use positively affect the use attitude of the elderly; perceived usefulness and user attitude positively affect the behavior intention of the elderly; perceived ease of use positively affects perceived usefulness; (2) perceived severity has a significant positive correlation with use attitude; perceived susceptibility and attitude to use have no significant impact; (3) perceived risk is negatively correlated with the use attitude and behavioral intention. The above-mentioned factors should be taken into consideration during the development of mobile healthcare applications for the aged to upgrade the overall service quality of mobile healthcare applications, thus enhancing the operational level of mobile healthcare applications and the health literacy of the aged

Numerical Simulation of Aerodynamic Performance of Off-grid Small Vertical Axis Wind Turbine

In this paper, a 2D off-grid small compact model of vertical axis wind turbine was established. The sliding grid technology, the RNG turbulence model and the Coupld algorithm was applied to simulate the unsteady value of the model's aerodynamic performance. Through the analysis on the flow field at difference moments, the rules about velocity fields, vortices distributions and the wind turbine's total torque were obtained. The results show that: the speed around wind turbine blades have obvious gradient, and the velocity distribution at different times show large differences in the computional domain. In the rotating domain vorticity is large. With away from the rotation domain, vorticity reduced quickly. In the process of rotating for vertical axis wind turbine, the wind turbine's total torque showed alternating positive and negative changes