7,617 research outputs found
Molecular analysis and phenotype characterization of the progeny of two antisense potato plants
Two transgenic potato lines csr2-1 and csr4-8, containing two different antisense constructs, csr2 and csr4, respectively, were crossed to investigate the possibility of achieving double transformants with combined effects of the two antisense transgenes on plant phenotypes and cellulose deposition. Molecular analysis revealed an expected segregation ratio of 1:1:1:1 of the four classes. Phenotype characterization revealed that offspring containing either one or both transgenes produced more tubers than the control plants but individual tubers were mostly smaller and had lesser weight than the control tubers
Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR
Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase
(CesA) genes, csr2 and csr4, respectively were crossed. The aim, amongst others, was to investigate the
possibility of generating double transformants to validate a hypothetical presence of the proteins of the
two CesA genes in the same cellulose synthase enzyme complex. SYBR-Green quantitative real-time
reverse transcription polymerase chain reaction (RT-PCR) assays were carried out on four CesA gene
transcripts (CesA1, 2, 3, and 4) in the wild type genetic background, and on the two antisense CesA gene
transcripts (CesA2 and 4) in the progeny resulting from the cross between the two transgenic potato
lines. The quantitative RT-PCR analyses revealed different expression patterns of the two CesA genes. The
CesA2 mRNA was shown to be relatively more abundant than CesA4 mRNA, regardless of the genetic
background, suggesting that the two proteins are not present in the same enzyme complex
Updating the phase diagram of the archetypal frustrated magnet Gd3Ga5O12
The applied magnetic field and temperature phase diagram of the archetypal
frustrated magnet, Gd3Ga5O12, has been reinvestigated using single crystal
magnetometry and polarised neutron diffraction. The updated phase diagram is
substantially more complicated than previously reported and can be understood
in terms of competing interactions with loops of spins, trimers and decagons,
in addition to competition and interplay between antiferromagnetic,
incommensurate and ferromagnetic order. Several additional distinct phase
boundaries are presented. The phase diagram centers around a multiphase
convergence to a single point at 0.9 T and ~ 0.35 K, below which, in
temperature, a very narrow magnetically disordered region exists. These data
illustrate the richness and diversity that arises from frustrated exchange on
the 3 dimensional hyperkagome lattice
An Achievement Test of Dynamic Control in Music
The use of the power level indicator with readings in decibels was standardized. Musically trained observers were tested on their respective instruments, untrained observers by voice. Their achievement test was compared with their motor capacity and sensory capacity tests. Conclusions were drawn in regard to the effect of pitch level, intensity level, instruments, and training
En fremgangsmĂĄde ved bestemmelse af normalomrĂĄde for haematologiske og klinisk kemiske parametre
No abstract availabl
Monte Carlo Study of Short-Range Order and Displacement Effects in Disordered CuAu
The correlation between local chemical environment and atomic displacements
in disordered CuAu alloy has been studied using Monte Carlo simulations based
on the effective medium theory (EMT) of metallic cohesion. These simulations
correctly reproduce the chemically-specific nearest-neighbor distances in the
random alloy across the entire Cu\$_x\$Au\$_{1-x}\$ concentration range. In the
random equiatomic CuAu alloy, the chemically specific pair distances depend
strongly on the local atomic environment (i.e. fraction of like/unlike nearest
neighbors). In CuAu alloy with short-range order, the relationship between
local environment and displacements remains qualitatively similar. However the
increase in short-range order causes the average Cu-Au distance to decrease
below the average Cu-Cu distance, as it does in the ordered CuAuI phase. Many
of these trends can be understood qualitatively from the different neutral
sphere radii and compressibilities of the Cu and Au atoms.Comment: 9 pages, 5 figures, 2 table
Chiral surfaces self-assembling in one-component systems with isotropic interactions
We show that chiral symmetry can be broken spontaneously in one-component
systems with isotropic interactions, i.e. many-particle systems having maximal
a priori symmetry. This is achieved by designing isotropic potentials that lead
to self-assembly of chiral surfaces. We demonstrate the principle on a simple
chiral lattice and on a more complex lattice with chiral super-cells. In
addition we show that the complex lattice has interesting melting behavior with
multiple morphologically distinct phases that we argue can be qualitatively
predicted from the design of the interaction.Comment: 4 pages, 4 figure
Chapter 25 Military Strategies for Samhandling in Unforeseen Situations
"The purpose of this chapter is twofold. Firstly, a rather paradoxical situation
will be discussed. Even though most military practitioners seem to agree
on the characteristics of war, two almost diametrically-opposed norms for how to
samhandle have arisen: a centralized one and a decentralized one. We argue that the
decentralized approach, called Auftragstaktik, is the most effective. In the second
part of the chapter we discuss the pedagogical challenge of educating personnel to
thrive in a decentralized organization, which must operate effectively in unforeseen
and threatening environments. Based on the teachings of Moltke the Elder (1800–
1891) and the much more recent writings of Torgersen, Steiro and Saeverot (2015),
we argue that a crucial step in educating for the unforeseen is to give the students
the opportunity to solve new problems by themselves, gaining experience that is, as
far as possible, self-generated and thereby becoming more aware and confident in
dealing with new situations.
Meanders: Exact Asymptotics
We conjecture that meanders are governed by the gravitational version of a
c=-4 two-dimensional conformal field theory, allowing for exact predictions for
the meander configuration exponent \alpha=\sqrt{29}(\sqrt{29}+\sqrt{5})/12, and
the semi-meander exponent {\bar\alpha}=1+\sqrt{11}(\sqrt{29}+\sqrt{5})/24. This
result follows from an interpretation of meanders as pairs of fully packed
loops on a random surface, described by two c=-2 free fields. The above values
agree with recent numerical estimates. We generalize these results to a score
of meandric numbers with various geometries and arbitrary loop fugacities.Comment: new version with note added in proo
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