The effect of cadmium on the bovine in vitro oocyte maturation and early embryo development

Common pollutants such as heavy metals and cadmium is among those with high environmental concerns. In vivo studies had shown that cadmium (Cd) causes oocyte degeneration and embryo mortality, and lowers pregnancy rates in mammals. However, there is limited information available about direct effects of Cd on oocyte maturation and/or embryo development. This study was aimed to investigate if Cd has any effect on the oocyte maturation and/or embryo development in vitro. Bovine COCs were collected from the slaughter house and cultured for 24 h in serum-free media only (Controls) or supplemented with 0.2, 2.0 and 20.0 μM CdCl2. At 24 h cumulus cell expansion was assessed in all COCs. COCs were either denuded and stained for determination of nuclear maturation or fertilized for assessment of subsequent embryo development. Cd at the lowest concentration (0.2 μM) did not affect any of the parameters studied. However, at higher concentrations (2.0 and 20.0 μM) it significantly (P < 0.05) reduced the percentage of fully-expanded COCs and significantly (P < 0.05) increased the percentage of partially and/or non-expanded COCs compared to controls and 0.2 μM. Cadmium at higher concentrations (2.0 and 20.0 μM) also significantly (P < 0.01) reduced the percentage of oocytes reaching metaphase II stage compared to controls and 0.2 μM. Post-fertilization cleavage rate in presumptive zygotes and blastocyst development significantly (P < 0.05) reduced 0.2, 2.0 and 20.0 μM CdCl2 compared to the controls (0.0 μM). In conclusion, these results suggest that Cd had direct detrimental effects on the bovine oocyte maturation and its developmental competence

Fish Community Analysis Using Multidirectional ROV Video Surveys in the Northwestern Gulf of Mexico

In this study, ROV surveys with multidirectional video were used to analyze the fish communities associated with artificial reef patches in the Rio Grande Valley artificial reef 13.7 km off the coast of South Padre Island, TX. Nine configurations of reef patches consisting of varying combinations and densities of concrete pyramid and low-profile modules were surveyed. The highest species diversity was found at patches with large deployments of both pyramids and low-profile modules. Total Red Snapper Lutjanus campechanus abundance did not differ between configurations, but the highest abundances of juvenile Red Snapper were found at configurations with one pyramid and one low-profile module and at the largest low-profile only sites. Negative correlations between juvenile Red Snapper and both Grey Triggerfish Balistes capriscus and adult Red Snapper were identified. The results of this study indicate that artificial reefs with separate habitat areas composed of 1) large deployments of mid-profile structures for older juvenile and adult Red Snapper and Grey Triggerfish and 2) isolated patches of low-profile habitat or a single mid-profile structure with associated low-profile habitat for small juvenile Red Snapper will be the most effective for fisheries enhancement and supporting diverse fish assemblages

Eine Methode zur Bromidbestimmung im Harn mit Hilfe einer ionensensitiven Elektrode

Peer Reviewe

Temporal Variability of Urinary Phthalate Metabolite Levels in Men of Reproductive Age

Phthalates are a family of multifunctional chemicals widely used in personal care and other consumer products. The ubiquitous use of phthalates results in human exposure through multiple sources and routes, including dietary ingestion, dermal absorption, inhalation, and parenteral exposure from medical devices containing phthalates. We explored the temporal variability over 3 months in urinary phthalate metabolite levels among 11 men who collected up to nine urine samples each during this time period. Eight phthalate metabolites were measured by solid-phase extraction–high-performance liquid chromatography–tandem mass spectrometry. Statistical analyses were performed to determine the between- and within-subject variance apportionment, and the sensitivity and specificity of a single urine sample to classify a subject’s 3-month average exposure. Five of the eight phthalates were frequently detected. Monoethyl phthalate (MEP) was detected in 100% of samples; monobutyl phthalate, monobenzyl phthalate, mono-2-ethylhexyl phthalate (MEHP), and monomethyl phthalate were detected in > 90% of samples. Although we found both substantial day-to-day and month-to-month variability in each individual’s urinary phthalate metabolite levels, a single urine sample was moderately predictive of each subject’s exposure over 3 months. The sensitivities ranged from 0.56 to 0.74. Both the degree of between- and within-subject variance and the predictive ability of a single urine sample differed among phthalate metabolites. In particular, a single urine sample was most predictive for MEP and least predictive for MEHP. These results suggest that the most efficient exposure assessment strategy for a particular study may depend on the phthalates of interest

Forecasting Forage Yields Using the ARIMA Model in Pastoral Areas of East Africa

Predicting forage supply is an age old quest for pastoralists, particularly in fragile and drought- prone areas of Africa. Traditional methods of forecasting forage used by many communities have become less effective due to climate change, frequent droughts and decline of grazing areas. Conflicts relating to available forage and water resources are increasing, because more marginal lands are put to crop production. A new forage forecasting technology has been developed that provides a comprehensive view of current forage condition (Stuth et al., 2004). A multiple species grazing land plant growth hydrology based model (PHYGROW) was parameterised with site-specific soil, plant community, grazer data that was spatially linked with satellite weather and predicted daily available forage (Rowan, 1995). The objective of this study was to explore use of the Auto-Regressive Integrated Moving-Average (ARIMA) procedure in forecasting a 30, 60 and 90-day available forage