Airframe noise prediction evaluation

The objective of this study is to evaluate the accuracy and adequacy of current airframe noise prediction methods using available airframe noise measurements from tests of a narrow body transport (DC-9) and a wide body transport (DC-10) in addition to scale model test data. General features of the airframe noise from these aircraft and models are outlined. The results of the assessment of two airframe prediction methods, Fink's and Munson's methods, against flight test data of these aircraft and scale model wind tunnel test data are presented. These methods were extensively evaluated against measured data from several configurations including clean, slat deployed, landing gear-deployed, flap deployed, and landing configurations of both DC-9 and DC-10. They were also assessed against a limited number of configurations of scale models. The evaluation was conducted in terms of overall sound pressure level (OASPL), tone corrected perceived noise level (PNLT), and one-third-octave band sound pressure level (SPL)

EGFR-Mediated Carcinoma Cell Metastasis Mediated by Integrin αvβ5 Depends on Activation of c-Src and Cleavage of MUC1

Receptor tyrosine kinases and integrins play an essential role in tumor cell invasion and metastasis. We previously showed that EGF and other growth factors induce human carcinoma cell invasion and metastasis mediated by integrin αvβ5 that is prevented by Src blockade [1]. MUC1, a transmembrane glycoprotein, is expressed in most epithelial tumors as a heterodimer consisting of an extracellular and a transmembrane subunit. The MUC1 cytoplasmic domain of the transmembrane subunit (MUC1.CD) translocates to the nucleus where it promotes the transcription of a metastatic gene signature associated with epithelial to mesenchymal transition. Here, we demonstrate a requirement for MUC1 in carcinoma cell metastasis dependent on EGFR and Src without affecting primary tumor growth. EGF stimulates Src-dependent MUC1 cleavage and nuclear localization leading to the expression of genes linked to metastasis. Moreover, expression of MUC1.CD results in its nuclear localization and is sufficient for transcription of the metastatic gene signature and tumor cell metastasis. These results demonstrate that EGFR and Src activity contribute to carcinoma cell invasion and metastasis mediated by integrin αvβ5 in part by promoting proteolytic cleavage of MUC1 and highlight the ability of MUC1.CD to promote metastasis in a context-dependent manner. Our findings may have implications for the use and future design of targeted therapies in cancers known to express EGFR, Src, or MUC1

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Omecamtiv mecarbil in chronic heart failure with reduced ejection fraction, GALACTIC‐HF: baseline characteristics and comparison with contemporary clinical trials

Aims: The safety and efficacy of the novel selective cardiac myosin activator, omecamtiv mecarbil, in patients with heart failure with reduced ejection fraction (HFrEF) is tested in the Global Approach to Lowering Adverse Cardiac outcomes Through Improving Contractility in Heart Failure (GALACTIC‐HF) trial. Here we describe the baseline characteristics of participants in GALACTIC‐HF and how these compare with other contemporary trials. Methods and Results: Adults with established HFrEF, New York Heart Association functional class (NYHA) ≥ II, EF ≤35%, elevated natriuretic peptides and either current hospitalization for HF or history of hospitalization/ emergency department visit for HF within a year were randomized to either placebo or omecamtiv mecarbil (pharmacokinetic‐guided dosing: 25, 37.5 or 50 mg bid). 8256 patients [male (79%), non‐white (22%), mean age 65 years] were enrolled with a mean EF 27%, ischemic etiology in 54%, NYHA II 53% and III/IV 47%, and median NT‐proBNP 1971 pg/mL. HF therapies at baseline were among the most effectively employed in contemporary HF trials. GALACTIC‐HF randomized patients representative of recent HF registries and trials with substantial numbers of patients also having characteristics understudied in previous trials including more from North America (n = 1386), enrolled as inpatients (n = 2084), systolic blood pressure < 100 mmHg (n = 1127), estimated glomerular filtration rate < 30 mL/min/1.73 m2 (n = 528), and treated with sacubitril‐valsartan at baseline (n = 1594). Conclusions: GALACTIC‐HF enrolled a well‐treated, high‐risk population from both inpatient and outpatient settings, which will provide a definitive evaluation of the efficacy and safety of this novel therapy, as well as informing its potential future implementation

Author Correction: Single-cell analysis of EphA clustering phenotypes to probe cancer cell heterogeneity

10.1038/s42003-020-01395-1COMMUNICATIONS BIOLOGY3

Single-cell analysis of EphA clustering phenotypes to probe cancer cell heterogeneity

10.1038/s42003-020-01136-4Communications Biology

Searching for Low-redshift Faint Galaxies with MMT/Hectospec

International audienceWe present redshifts for 2753 low-redshift galaxies between 0.03 ≲ zspec ≲ 0.5 with 18 ≤ r ≤ 22 obtained with Hectospec at the Multi-Mirror Telescope. The observations targeted the XMM-LSS, ELAIS-N1 and DEEP2-3 fields, each of which covers ~1 deg2. These fields are also part of the recently completed Canada-France-Hawaii Telescope Large Area U-band Deep Survey and ongoing Hyper Suprime-Cam deep fields surveys. The efficiency of our technique for selecting low-redshift galaxies is confirmed by the redshift distribution of our sources. In addition to redshifts, these high signal-to-noise ratio spectra are used to measure ages, metallicities, and nuclear activity levels. In combination with the photometric catalog in u, g, r, i, z, y down to 27 AB mag, we are able to study the galaxy population down to stellar masses of ~108 M⊙. This paper presents the observational strategy, the reduction procedure and properties of the galaxy sample. (The catalog can be accessed through the survey's website at http://mips.as.arizona.edu/~cnaw/Faint_Low_z/.