TOXICIDAD AGUDA Y LESIONES HISTOPATOLÓGICAS EN CACHAMA BLANCA (Piaractus brachypomus) EXPUESTAS A LA MEZCLA DE HERBICIDA ROUNDUP® MÁS SURFACTANTE COSMOFLUX® 411F

Objetivo. Determinar la concentración letal 50 (CL 50 ) y lesiones anatomopatológicas inducidas por la exposición a la mezcla del herbicida Roundup® más el surfactante Cosmoflux® 411F en juveniles de cachama blanca (Piaractus brachypomus). Materiales y métodos. Ejemplares de 44.5 ± 3.7 g de peso fueron expuestos por 96 h a varias concentraciones de la mezcla herbicida más surfactante. Se registró la mortalidad al cabo de las 96 h y se tomaron muestras de tejidos para evaluación histopatológica. Resultados. La CL 50 fue de 23.42 mg.l -1 para el Roundup® y de 0.19 mg.l -1 para el Cosmoflux® 411F (

Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research

Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. Although there has been a recent increase in CHIKV research, many of these in vitro studies have used a wide range of cell lines which are not physiologically relevant to CHIKV infection in vivo. In this study, we aimed to evaluate a panel of cell lines to identify a subset that would be both representative of the infectious cycle of CHIKV in vivo, and amenable to in vitro applications such as transfection, luciferase assays, immunofluorescence, western blotting and virus infection. Based on these parameters we selected four mammalian and two mosquito cell lines, and further characterised these as potential tools in CHIKV research

The Zwicky Transient Facility: System Overview, Performance, and First Results

The Zwicky Transient Facility (ZTF) is a new optical time-domain survey that uses the Palomar 48 inch Schmidt telescope. A custom-built wide-field camera provides a 47 deg^2 field of view and 8 s readout time, yielding more than an order of magnitude improvement in survey speed relative to its predecessor survey, the Palomar Transient Factory. We describe the design and implementation of the camera and observing system. The ZTF data system at the Infrared Processing and Analysis Center provides near-real-time reduction to identify moving and varying objects. We outline the analysis pipelines, data products, and associated archive. Finally, we present on-sky performance analysis and first scientific results from commissioning and the early survey. ZTF's public alert stream will serve as a useful precursor for that of the Large Synoptic Survey Telescope