Pollia secundiflora Bakh. fil.

原著和名: コヤブメウガ科名: ツユクサ科 = Commelinaceae採集地: 鹿児島県 沖永良部島 大山 (大隅 沖永良部島 大山)採集日: 1971/11/8採集者: 萩庭丈壽整理番号: JH019225国立科学博物館整理番号: TNS-VS-96922

Additional file 2: Table S1. of Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism

125 proteins differentially regulated by MeHg. (XLSX 94 kb

Volcano plot.

<p>The significance of the relative regulation of 394 proteins was inspected using (1) a paired two-sided t-test (y axis) and (2) by estimating the probability for the regulation derived from the background (x axis). Proteins are clustered into four categories based on the statistical test passing the threshold (see text for details).</p

Protein regulation vs. abundance index.

<p>The protein regulation is plotted against the abundance index and every protein is classified according to the result of the statistical tests.</p

Experimental protocol.

<p>A peripheral venous catheter was inserted in the cubital fossa of subject for blood sampling. The subject rested for 14 min reclined on a bench before the baseline sample was drawn. The blood pressure cuff was inflated to 200 mmHg for 5 min before being released. Blood samples were drawn at 1 and 4 min into reperfusion from the ipsilateral arm. Blood samples were centrifuged to collect plasma which was stored at −80°C. Before analysis, all six reperfusion samples were pooled for each subject.</p

Analysis workflow.

<p>Plasma samples were depleted by a MARS Hu-14 column and subsequently concentrated by 3 kDa ultracentrifugation filters. Next, samples were reduced, cysteine blocked and trypsin digested before iTRAQ labeling. The iTRAQ labeled peptides were fractioned into 60 fractions using a mixed-mode reverse phase anion exchanger. Finally, fractions were analyzed on an LTQ-Orbitrap Velos Pro connected to a Dionex Ultimate NCR-3000RS LC system.</p

Repartition on every iTRAQ channel of the samples at baseline and after RIPC for the six donors.

<p>Repartition on every iTRAQ channel of the samples at baseline and after RIPC for the six donors.</p

Additional file 1: of Long-term smoking alters abundance of over half of the proteome in bronchoalveolar lavage cell in smokers with normal spirometry, with effects on molecular pathways associated with COPD

Table S1. Clinical characteristics of subjects, stratified by gender. Table S2. Proteins significantly altered between Smoker vs Never-smoker groups. Table S3. Proteins significantly altered between female Smoker vs Never-smoker groups. Table S4. Proteins significantly altered between male Smoker vs Never-smoker groups. Table S5. Significantly enriched pathways and associated proteins when comparing Smoker and Never-smoker groups. Table S6. Significantly enriched pathways following stratification by gender when comparing Smoker and Never-smoker groups. (XLSX 1321 kb

Additional file 2: of Long-term smoking alters abundance of over half of the proteome in bronchoalveolar lavage cell in smokers with normal spirometry, with effects on molecular pathways associated with COPD

Figure S1. The OPLS-DA modeling parameters for joint gender, female and male Smoker vs. Never-smoker. Permutation test was performed 200 times for each model. Figure S2. Leukocyte transendothelial migration was significantly altered in joint smokers. ITGAM, P11215, Integrin alpha-M (CD11b); ITGB2, P05107, Integrin beta-2 (CD18); PECAM1, P16284, Platelet endothelial cell adhesion molecule; JAM-A, Q9Y624, Junctional adhesion molecule A; MLC-2, O14950, Myosin regulatory light chain 12B; CDC42, P60953, Cell division control protein 42 homolog; Actin, P60709, actin cytoplasmic 1; α-actin, P12814, O43707, α-actinin-1, α-actinin-4, respectively; NOX2, P04839, Cytochrome b-245 heavy chain; p40phox, Q15080, Neutrophil cytosol factor 4; RAC2, P15153, Ras-related C3 botulinum toxin substrate 2; RAP1A, P62834, Ras-related protein Rap-1A. (DOC 1606 kb

Additional file 1: of Proteomic profiling of lung immune cells reveals dysregulation of phagocytotic pathways in female-dominated molecular COPD phenotype

Supplementary Methods. Figure S1. Analysis of Share and Unique Structure (SUS) between OPLS-DA models of female Smoker vs COPD (x-axis) and Never-smoker vs ex-smoker with COPD (exCOPD) (y-axis). Figure S2. Multivariate sensitivity analysis of the impact of menopausal status on proteomic profiling in female COPD patients. Figure S3. The percentage of CT attenuation values <−950 HU in the Smoker and COPD groups, stratified by gender. (DOC 1849 kb