原著和名: コヤブメウガ科名: ツユクサ科 = Commelinaceae採集地: 鹿児島県 沖永良部島 大山 (大隅 沖永良部島 大山)採集日: 1971/11/8採集者: 萩庭丈壽整理番号: JH019225国立科学博物館整理番号: TNS-VS-96922

Anders Goksøyr (3124773)

Eystein Oveland (223307)

Fekadu Yadetie (4636)

Frode Berven (2007676)

Hilde Garberg (644995)

Odd Karlsen (3586319)

Silje Bjørneklett (3586322)

BMC Genomics

PubMed

Full list of significantly enriched KEGG, GO (BP, MF and CC) and Interpro protein domains from STRING network analysis. (XLS 50 kb

FigShare

Additional file 5: Table S4. of Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism

Full list of 1143 quantified proteins and associated data from label-free proteomics analysis of liver protein samples from controls (n = 10), 0.5 mg/kg BW MeHg (n = 9) and 2 mg/kg BW MeHg (n = 9) fish. (XLSX 610 kb

Additional file 3: Table S2. of Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism

Background: Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver proteome of Atlantic cod (Gadus morhua) exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. Results: Out of a toltal of 1143 proteins quantified, 125 proteins were differentially regulated between MeHg-treated samples and controls. Using various bioinformatics tools, we performed gene ontology, pathway and network enrichment analysis, which indicated that proteins and pathways mainly related to energy metabolism, antioxidant defense, cytoskeleton remodeling, and protein synthesis were regulated in the hepatic proteome after MeHg exposure. Comparison with previous gene expression data strengthened these results, and further supported that MeHg predominantly affects many energy metabolism pathways, presumably through its strong induction of oxidative stress. Some enzymes known to have functionally important oxidation-sensitive cysteine residues in other animals are among the differentially regulated proteins, suggesting their modulations by MeHg-induced oxidative stress. Integrated analysis of the proteomics dataset combined with previous gene expression dataset showed a more pronounced effect of MeHg on amino acid, glucose and fatty acid metabolic pathways, and suggested possible interactions of the cellular energy metabolism and antioxidant defense pathways. Conclusions: MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver. The energy pathways appear to be modulated through MeHg-induced oxidative stress, possibly mediated by oxidation sensitive enzymes

Yadetie, Fekadu

Bjørneklett, Silje

Garberg, Hilde K

Oveland, Eystein

Berven, Frode

Goksøyr, Anders

Karlsen, Odd André

NORA - Norwegian Open Research Archives

Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism

125 proteins differentially regulated by MeHg. (XLSX 94 kb

Additional file 2: Table S1. of Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism

Fekadu Yadetie

Silje Bjørneklett

Hilde Kristin Garberg

Eystein Oveland

Frode Berven

Anders Goksøyr

Odd André Karlsen

Springer - Publisher Connector

Background: Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver proteome of Atlantic cod (Gadus morhua) exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. Results: Out of a toltal of 1143 proteins quantified, 125 proteins were differentially regulated between MeHg-treated samples and controls. Using various bioinformatics tools, we performed gene ontology, pathway and network enrichment analysis, which indicated that proteins and pathways mainly related to energy metabolism, antioxidant defense, cytoskeleton remodeling, and protein synthesis were regulated in the hepatic proteome after MeHg exposure. Comparison with previous gene expression data strengthened these results, and further supported that MeHg predominantly affects many energy metabolism pathways, presumably through its strong induction of oxidative stress. Some enzymes known to have functionally important oxidation-sensitive cysteine residues in other animals are among the differentially regulated proteins, suggesting their modulations by MeHg-induced oxidative stress. Integrated analysis of the proteomics dataset combined with previous gene expression dataset showed a more pronounced effect of MeHg on amino acid, glucose and fatty acid metabolic pathways, and suggested possible interactions of the cellular energy metabolism and antioxidant defense pathways. Conclusions: MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver. The energy pathways appear to be modulated through MeHg-induced oxidative stress, possibly mediated by oxidation sensitive enzymes.publishedVersio

University of Bergen

English

Full list of significantly enriched top 50 GO BP and MF and all significant MetaCore processes network terms and the associated proteins. (XLSX 73 kb

Additional file 4: Table S3. of Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism

Crossref

https://figshare.com/articles/Additional_file_3_Table_S2_of_Quantitative_analyses_of_the_hepatic_proteome_of_methylmercury-exposed_Atlantic_cod_Gadus_morhua_suggest_oxidative_stress-mediated_effects_on_cellular_energy_metabolism/4473800