Markers of physiological stress during exercise under conditions of normoxia, normobaric hypoxia, hypobaric hypoxia and genuine high altitude.

Purpose To investigate whether there is a differential response at rest and following exercise to conditions of genuine high altitude (GHA), normobaric hypoxia (NH), hypobaric hypoxia (HH) and normobaric normoxia (NN). Method Markers of sympathoadrenal and adrenocortical function (plasma normetanephrine [PNORMET], metanephrine [PMET], cortisol), myocardial injury (highly sensitive cardiac troponin T [hscTnT]) and function (N-terminal brain natriuretic peptide [NT-proBNP]) were evaluated at rest and with exercise under NN, at 3375 m in the Alps (GHA) and at equivalent simulated altitude under NH and HH. Participants cycled for 2 hours {15 minute warm-up, 105 minutes at 55% Wmax (maximal workload)} with venous blood samples taken prior (T0), immediately following (T120) and 2 hours post-exercise (T240). Results Exercise in the three hypoxic environments produced a similar pattern of response with the only difference between environments being in relation to PNORMET. Exercise in NN only induced a rise in PNORMET and PMET. Conclusion Biochemical markers that reflect sympathoadrenal, adrenocortical and myocardial responses to physiological stress demonstrate significant differences in the response to exercise under conditions of normoxia versus hypoxia while NH and HH appear to induce broadly similar responses to GHA and may therefore be reasonable surrogates

Fish Population Ecology and Ecological Risk Assessment

Density-dependent processes are crucial in the regulation of fish populations and strongly influence their resilience to exploitation and exposure to toxic chemicals. Multiple density-dependent processes occur at different stages in the life-cycle of fish, and a general pattern of such processes in the ontogeny of fish has been suggested but not clearly demonstrated in natural populations. This thesis aimed to provide a detailed experimental assessment of density-dependent processes through the entire life-cycle, using laboratory and semi-natural populations of zebrafish, Danio rerio, and to explore the implications of these processes for the ecological risk assessment of endocrine disrupting chemicals using an individual-based population model. Results clearly demonstrate the importance of density-dependent mortality in the early juvenile life-stage and density-dependent growth in the late juvenile and adult life-stages consistent with evidence from wild populations of much larger wild species, suggesting the existence of general ontogenetic patterns of density dependence that are invariant to maximum size. Patterns of density dependence found in populations of zebrafish under semi-natural conditions in Bangladesh were similar to those observed in the laboratory, except that the absolute strength of density dependence was higher and consequently, carrying capacity lower, by about two orders of magnitude in the semi-natural populations. A conclusion from these studies is that these patterns of density dependence are applicable generally across the teleost taxa due to developmental similarities. The population model incorporating these patterns of density dependence showed that density dependence compensated for reasonably high levels of disruption for many individual-level endpoints currently used in risk assessment, including fecundity and sex ratio. This indicates that current risk assessment practices are highly conservative and the inclusion of population models such as developed here for zebrafish, could enhance the scientific basis and ecological realism of laboratory derived data used in risk analysis

Fish population ecology and ecological risk assessment

Density-dependent processes are crucial in the regulation of fish populations and strongly influence their resilience to exploitation and exposure to toxic chemicals. Multiple density-dependent processes occur at different stages in the life-cycle of fish, and a general pattern of such processes in the ontogeny of fish has been suggested but not clearly demonstrated in natural populations. This thesis aimed to provide a detailed experimental assessment of density-dependent processes through the entire life-cycle, using laboratory and semi-natural populations of zebrafish, Danio rerio, and to explore the implications of these processes for the ecological risk assessment of endocrine disrupting chemicals using an individual-based population model. Results clearly demonstrate the importance of density-dependent mortality in the early juvenile life-stage and density-dependent growth in the late juvenile and adult life-stages consistent with evidence from wild populations of much larger wild species, suggesting the existence of general ontogenetic patterns of density dependence that are invariant to maximum size. Patterns of density dependence found in populations of zebrafish under semi-natural conditions in Bangladesh were similar to those observed in the laboratory, except that the absolute strength of density dependence was higher and consequently, carrying capacity lower, by about two orders of magnitude in the semi-natural populations. A conclusion from these studies is that these patterns of density dependence are applicable generally across the teleost taxa due to developmental similarities. The population model incorporating these patterns of density dependence showed that density dependence compensated for reasonably high levels of disruption for many individual-level endpoints currently used in risk assessment, including fecundity and sex ratio. This indicates that current risk assessment practices are highly conservative and the inclusion of population models such as developed here for zebrafish, could enhance the scientific basis and ecological realism of laboratory derived data used in risk analysis.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Density-dependent processes in the life history of fishes: evidence from laboratory populations of zebrafish Danio rerio.

Population regulation is fundamental to the long-term persistence of populations and their responses to harvesting, habitat modification, and exposure to toxic chemicals. In fish and other organisms with complex life histories, regulation may involve density dependence in different life-stages and vital rates. We studied density dependence in body growth and mortality through the life-cycle of laboratory populations of zebrafish Danio rerio. When feed input was held constant at population-level (leading to resource limitation), body growth was strongly density-dependent in the late juvenile and adult phases of the life-cycle. Density dependence in mortality was strong during the early juvenile phase but declined thereafter and virtually ceased prior to maturation. Provision of feed in proportion to individual requirements (easing resource limitation) removed density dependence in growth and substantially reduced density dependence in mortality, thus indicating that 'bottom-up' effects act on growth as well as mortality, but most strongly on growth. Both growth and mortality played an important role in population regulation, with density-dependent growth having the greater impact on population biomass while mortality had the greatest impact on numbers. We demonstrate a clear ontogenic pattern of change in density-dependent processes within populations of a very small (maximum length 5 mm) fish, maintained in constant homogeneous laboratory conditions. The patterns are consistent with those distilled from studies on wild fish populations, indicating the presence of broad ontogenic patterns in density-dependent processes that are invariant to maximum body size and hold in homogeneous laboratory, as well as complex natural environments

Parameter values for zebrafish survival model under two different feed regimes.

<p>Observed (○) and modelled (line) values for parameters <i>a</i> and <i>b</i> in the Beverton-Holt survival model for constant feed (A & C) and individual requirement feed regimes (B & D). Parameter <i>A</i> denotes density-independent mortality and parameter <i>B</i> denotes density-dependent mortality.</p

Dynamic simulation results.

<p>The results of dynamic simulations into the growth and mortality of zebrafish from hatch to 130 dpf maintained under constant feeding conditions. Final population abundance (A) and final population biomass (B) were calculated under full density dependence (dashed red), density dependence in mortality only (dotted blue), density dependence in growth only (dashed green) and no density dependence (solid black).</p

Average number of zebrafish under two different feed regimes.

<p>Average number of individual fish (±s.e) stocked at three different densities (low density - •, mid density -▴, high density - ▪) over 130 dpf under a constant feed regime (A) and an individual requirement feed regime (B).</p