Design ofExperiment on Ultra Violet Curing Die Attach Filmby Using Taguchi Method to Optimize Quality

The highly growth on semiconductor manufacturing field makes the expertise on the semiconductor manufacturer industry keep on struggle to create higher speed, smaller, highly integrated and more reliable and powerful chips for various needs. The new technology in making chips process continuously developed to produce goods semiconductor. Product and process optimization was a crucial part in manufacturing production line to produce high volume throughput with reducing in lead times, engineering changes and development cost but with higher quality. Engineering particularly decisions be the most important side in making the process properly in reaching the zero defect target. Every single thing has to be concerned, whether it is from technical parts to managerial parts in order to ensure the process going smoothly and efficiently with excellent quality. For this project, I am going deeper inside the chips assembling process whereby the post Ultra Violet curing process on Die Attach film, DAF tape have been experimented by using Taguchi approach for the process to produce better chips on stacked die package. An application of experimental design, based upon the works of Dr.Genichi Taguchi was conducted at the die attach film (DAF) curing operation. The experiment focused on parameter design, the determination of the DAF operation parameters that significantly affect the quality and semiconductor chip reliability ofthe wafer mounted on wafer ring during curing and selecting the optimum parameter level combination. In semiconductor manufacturing industry, the usage of epoxy resin bleed is no longer convenient in producing thin die stacked package with high volume. Hence, industry in about to move to the Die attach film (DAF) tape type to replace with the epoxy resin bleed. The new process should the tape go through is UV curing process, indeed the characteristics ofthe tape adhesion affecting much to yield and quality. Parameter optimization has resulted to a reduction in die defects and adjustment of the mean to the nominal of zero defects for all the quality characteristics. Continuations on the optimizing UV curing DAF tape process is moving on with the inspection data gathered which is die shear strength, top and flip side chipped and tape residue whiskering. Furthermore, evaluation effect onto the process which is the die shear strength test, inspection has been taken into account particularly. Proper planning on the control factors and responses for the process were managed accordingly by using Taguchi approach for necessary information. Implementation of the optimum condition has resulted to about 50% reduction of defects and failures

Generation and characterisation of human umbilical cord derived mesenchymal stem cells by explant method

Mesenchymal stem cells (MSCs) derived from human umbilical cord (UC) have been considered as an important tool for treating various malignancies, tissue repair and organ regeneration. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are better alternative to MSCs that derived from bone marrow (BM-MSCs) as they are regarded as medical waste with little ethical concern for research and easily culture-expanded. In this present study, the foetal distal end of human UC was utilised to generate MSC by explant method. Upon in vitro culture, adherent cells with fibroblastic morphology were generated with rapid growth kinetics. Under the respective inductive conditions, these cells were capable of differentiating into adipocytes and osteocytes; express an array of standard MSC’s surface markers CD29, CD73, CD90, CD106 and MHC-class I. Further assessment of immunosuppression activity revealed that MSCs generated from UC had profoundly inhibited the proliferation of mitogen-activated T lymphocytes in a dosedependent manner. The current laboratory findings have reinforced the application of explant method to generate UCMSCs thus, exploring an ideal platform to fulfil the increasing demand of MSCs for research and potential clinical use

Molecular and serological detection of occult hepatitis B virus among healthy hepatitis B surface antigen-negative blood donors in Malaysia.

Background: Occult hepatitis B infections are becoming a major global threat, but the available data on its prevalence in various parts of the world are often divergent. Objective: This study aimed to detect occult hepatitis B virus in hepatitis B surface antigen-negative serum using anti-HBc as a marker of previous infection. Patient and Methods: A total of 1000 randomly selected hepatitis B surface antigen-negative sera from blood donors were tested for hepatitis B core antibody and hepatitis B surface antibody using an ELISA and nested polymerase chain reaction was done using primers specific to the surface gene (S-gene). Results: Of the 1000 samples 55 (5.5%) were found to be reactive, of which 87.3% (48/55) were positive for hepatitis B surface antibody, indicating immunity as a result of previous infection however, that does not exclude active infection with escaped mutant HBV. Nested PCR results showed the presence of hepatitis B viral DNA in all the 55 samples that were positive for core protein, which is in agreement with the hepatitis B surface antibody result. Conclusion: This study reveals the 5.5% prevalence of occult hepatitis B among Malaysian blood donors as well as the reliability of using hepatitis B core antibody in screening for occult hepatitis B infection in low endemic, low socioeconomic settings

Design of Experiment on Ultra Violet Curing Die Attach Film by Using Taguchi Method to Optimize Quality

The highly growth on semiconductor manufacturing field makes the expertise on the semiconductor manufacturer industry keep on struggle to create higher speed, smaller, highly integrated and more reliable and powerful chips for various needs. The new technology in making chips process continuously developed to produce goods semiconductor. Product and process optimization was a crucial part in manufacturing production line to produce high volume throughput with reducing in lead times, engineering changes and development cost but with higher quality. Engineering particularly decisions be the most important side in making the process properly in reaching the zero defect target. Every single thing has to be concerned, whether it is from technical parts to managerial parts in order to ensure the process going smoothly and efficiently with excellent quality. For this project, I am going deeper inside the chips assembling process whereby the post Ultra Violet curing process on Die Attach film, DAF tape have been experimented by using Taguchi approach for the process to produce better chips on stacked die package. An application of experimental design, based upon the works of Dr.Genichi Taguchi was conducted at the die attach film (DAF) curing operation. The experiment focused on parameter design, the determination of the DAF operation parameters that significantly affect the quality and semiconductor chip reliability of the wafer mounted on wafer ring during curing and selecting the optimum parameter level combination. In semiconductor manufacturing industry, the usage of epoxy resin bleed is no longer convenient in producing thin die stacked package with high volume. Hence, industry in about to move to the Die attach film (DAF) tape type to replace with the epoxy resin bleed. The new process should the tape go through is UV curing process, indeed the characteristics of the tape adhesion affecting much to yield and quality. Parameter optimization has resulted to a reduction in die defects and adjustment of the mean to the nominal of zero defects for all the quality characteristics. Continuations on the optimizing UV curing DAF tape process is moving on with the inspection data gathered which is die shear strength, top and flip side chipped and tape residue whiskering. Furthermore, evaluation effect onto the process which is the die shear strength test, inspection has been taken into account particularly

Molecular and serological detection of occult hepatitis B virus among healthy hepatitis B surface antigen-negative blood donors in Malaysia

Background: Occult hepatitis B infections are becoming a major global threat, but the available data on its prevalence in various parts of the world are often divergent.Objective: This study aimed to detect occult hepatitis B virus in hepatitis B surface antigen-negative serum using anti-HBc as a marker of previous infection. Patient and methods: A total of 1000 randomly selected hepatitis B surface antigen-negative sera from blood donors were tested for hepatitis B core antibody and hepatitis B surface antibody using an ELISA and nested polymerase chain reaction was done using primers specific to the surface gene (S-gene). Results: Of the 1000 samples 55 (5.5%) were found to be reactive, of which 87.3% (48/55) were positive for hepatitis B surface antibody, indicating immunity as a result of previous infection however, that does not exclude active infection with escaped mutant HBV. Nested PCR results showed the presence of hepatitis B viral DNA in all the 55 samples that were positive for core protein, which is in agreement with the hepatitis B surface antibody result. Conclusion: This study reveals the 5.5% prevalence of occult hepatitis B among Malaysian blood donors as well as the reliability of using hepatitis B core antibody in screening for occult hepatitis B infection in low endemic, low socioeconomic settings