Summary of Coral Cay Conservation's habitat mapping data from Utila, Honduras

IIThe coral reefs of Honduras are of vital national and international importance,both ecologically and economically, but are threatened because of rapid economicand population growth.? During work on Utila between 1999 and 2000 (the ?Bay Islands 2000? project),Coral Cay Conservation developed a programme of surveys, training andconservation education aimed at assessing the status of local reefs and improvingenvironmental awareness amongst neighbouring communities.? This summary report provides an overview of the habitat mapping data collectedby the Bay Islands 2000 project.? CASA provided software, hardware and skills, on a charitable basis to ensure thatthe data collected by CCC could be developed into a GIS, not only for mappingthe status of the coral reefs of Honduras, but also to provide analysis of the aerialextent of these reefs.? Data were collected within individual ?study areas?, to facilitate analysis at a rangeof spatial scales, and utilised the CCC standard baseline survey technique for therapid assessment of the characteristics of reef communities. The surveys,therefore, utilised a series of transects, perpendicular to the reef.? Baseline transects discriminated nine benthic and six geomorpholgical classeswhich indicates Utila has a high habitat diversity. Habitat diversity is importantsince the number of habitat types has been shown to be a good representation ofspecies biodiversity.? The nine benthic classes that were distinguished were all relatively coral poorbecause of a suite of relatively long-term local and regional factors, exacerbatedby the combination of Hurricane Mitch and coral bleaching in 1995 and 1998.? Damselfish were the most abundant reef associated fish recorded during baselinetransect surveys. Commercially important fish were less abundant that wouldnormally be expected in unfished systems.? A recurring pattern in the baseline transect data was the greater abundance anddiversity of fish in coral rich classes. However, although the link between fishabundance and coral cover was clear, not all species were necessarily mostabundant in the most coral rich areas.? Invertebrates were generally uncommon, partly because of fishing pressure, andthe abundances of many invertebrate taxa were correlated with coral cover.? A habitat map is presented within this report as an indication of the distribution ofhabitat types around Utila.? Using the map, estimates of areal extents of each benthic class and habitat type areinstructive. For example, there is only approximately 27 km2 of reefal habitatsaround Utila. Furthermore, the area supporting the most coral rich benthic classesis only approximately 4 km2 (15%). These statistics both highlight the damagecaused by the bleaching event and Hurricane Mitch and other anthropogenicimpacts and the need to conserve remaining coral rich areas.? If further reserves were to be created, it would be important to try to protect arange of reef and habitat types. For this reason, it appears that the Turtle HarbourWildlife Refuge is well placed since this areas includes a wide range of habitattypes. However, placement of reserves in Utila should favour relatively coral richhabitats over sand dominated areas.? This study led to six recommendations:Summary Utila habitat mapping reportIII? One or more agencies should collect additional ground-truthing data fromaround Utila to facilitate both classification of currently ?Unknown? polygonsand an accuracy assessment of the map.? Establish an integrated GIS and associated meta-database for Utila, includingdata from the Bay Islands 2000 project.? Examine the potential of using data collected by the Bay Islands 2000 projectas the basis of national habitat classification scheme and subsequent nationalhabitat map.? Continue to aim to establish one or more additional multiple use marineprotected areas around Utila, with an integrated monitoring programme tomeasure their efficacy, and strengthen the enforcement of regulations in theTurtle Harbour Wildlife Sanctuary. Establish regulations, and enforce existinglegislation, to minimise the detrimental effects of coastal development on reefhealth.? Additional marine reserves in Utila should integrate factors such as thepreference of many fish species for coral rich habitats and the protection ofareas incorporating a range of habitat types, including mangroves and seagrassbeds, in order to allow for nursery areas, ontogenetic shifts and species thatrely on non-coral rich habitats. The corollary of the preference of fish speciesfor coral rich habitats is to protect coral cover within the reserves.? The reef on the south coast of Utila appears to be a good candidate forprotection because it is relatively sheltered from storm and hurricane damage

Unusual placement of intrathecal baclofen pumps: report of two cases

Intrathecal baclofen delivery via implantable pump represents an important modality for symptomatic relief in patients with chronic spasticity. Pumps are routinely implanted subcutaneously in the anterior abdominal wall. We describe two unusual cases where skin-related complications necessitated revision surgery in order to relocate the pump to alternative sites. The first patient was an international power canoeist, whose strenuous exercise programme interfered with his pump’s original siting. The second patient was a cachectic university student with a history of cerebral palsy, who maintained low body mass despite attempted weight gain. The relocation of these two intrathecal devices to the medial compartment of the right thigh and right iliac fossa, respectively, is described

Novel variants provide differential stabilisation of human equilibrative nucleoside transporter 1 states

Human equilibrative nucleoside transporters represent a major pharmaceutical target for cardiac, cancer and viral therapies. Understanding the molecular basis for transport is crucial for the development of improved therapeutics through structure-based drug design. ENTs have been proposed to utilise an alternating access mechanism of action, similar to that of the major facilitator superfamily. However, ENTs lack functionally-essential features of that superfamily, suggesting that they may use a different transport mechanism. Understanding the molecular basis of their transport requires insight into diverse conformational states. Differences between intermediate states may be discrete and mediated by subtle gating interactions, such as salt bridges. We identified four variants of human equilibrative nucleoside transporter isoform 1 (hENT1) at the large intracellular loop (ICL6) and transmembrane helix 7 (TM7) that stabilise the apo-state (T-m 0.7-1.5 degrees C). Furthermore, we showed that variants K263A (ICL6) and I282V (TM7) specifically stabilise the inhibitor-bound state of hENT1 (T-m 5.0 +/- 1.7 degrees C and 3.0 +/- 1.8 degrees C), supporting the role of ICL6 in hENT1 gating. Finally, we showed that, in comparison with wild type, variant T336A is destabilised by nitrobenzylthioinosine (T-m -4.7 +/- 1.1 degrees C) and binds it seven times worse. This residue may help determine inhibitor and substrate sensitivity. Residue K263 is not present in the solved structures, highlighting the need for further structural data that include the loop regions.Peer reviewe

Izolacija i sposobnost hvatanja slobodnih radikala cijanidin 3-O-glikozida iz plodova Ribes biebersteinii Berl.

The reversed-phase preparative high performance liquid chromatographic purification of the methanol extract of the fruits of Ribes biebersteinii Berl. (Grossulariaceae) afforded five cyanidin glycosides, 3-O-sambubiosyl-5-O-glucosyl cyanidin (1), cyanidin 3-O-sambubioside (2), cyanidin 3-O-glucoside (3), cyanidin 3-O-(2G-xylosyl)-rutinoside (4) and cyanidin 3-O-rutinoside (5). They showed considerable free-radical-scavenging properties in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay with the RC50 values of 9.29 × 106, 9.33 × 106, 8.31 × 106, 8.96 × 106 and 9.55 × 106 mol L1, respectively. The structures of these compounds were elucidated by various chemical hydrolyses and spectroscopic means. The total anthocyanin content was 1.9 g per 100 g dried fruits on cyanidin 3-glucoside basis.Pet cijanidin glikozida, 3-O-sambubiozil-5-O-glukozil cijanidin (1), cijanidin 3-O-sambubiozid (2), cijanidin 3-O-glukozid (3), cijanidin 3-O-(2G-ksilozil)-rutinozid (4) i cijanidin 3-O-rutinosid (5) izolirani su iz metanolnog ekstrakta plodova Ribes biebersteinii Berl. (Grossulariaceae) koristeći reverzno-faznu preparativnu tekućinsku kromatografiju visoke učinkovitosti. Cijanidin glikozidi pokazali su sposobnost hvatanja slobodnih radikala u pokusu s 2,2-difenil-1-pikrilhidrazilom (DPPH). Dobivene su sljedeće RC50 vrijednosti: 9,29 × 106, 9,33 × 106, 8,31 × 106, 8,96 × 106, odnosno 9,55 × 106 mol L1. Strukture glikozida određene su kemijskom hidrolizom i spektroskopijom masa. Ukupni sadržaj antocijanina bio je 1,9 g na 100 g suhih plodova preračunato na cijanidin 3-glukozid

Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbs

Context: Crataegus monogyna L. (Rosaceae) (CM), Equisetum telmateia L. (Equisataceae) (ET), Geranium purpureum Vil. (Geraniaceae) (GP), Mentha suaveolens Ehrh. (Lamiaceae) (MS), and Lavandula stoechas L. spp. luisieri (Lamiaceae) (LS) are all medicinal. Objective: To evaluate the antioxidant, antiproliferative and antimicrobial activities of plant extracts and quantify individual phenolics and zinc. Material and methods: Aerial part extracts were prepared with water (W), ethanol (E) and an 80% mixture (80EW). Antioxidant activity was measured with TAA, FRAP and RP methods. Phenolics were quantified with a HPLC. Zinc was quantified using voltammetry. Antibacterial activity (after 48 h) was tested using Enterococcus faecalis, Bacillus cereus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Listeria monocytogenes. Antiproliferative activity (after 24 h) was tested using HEP G2 cells and fibroblasts. Results: Solvents influenced results; the best were E and 80EW. GP had the highest antioxidant activity (TAA and FRAP of 536.90mg AAE/g dw and 783.48mg TE/g dw, respectively). CM had the highest zinc concentration (37.21 mg/kg) and phenolic variety, with neochlorogenic acid as the most abundant (92.91 mg/100 g dw). LS was rich in rosmarinic acid (301.71 mg/100 g dw). GP and LS inhibited the most microorganisms: B. cereus, E. coli and S. aureus. GP also inhibited E. faecalis. CM had the lowest MIC: 5830 mu g/mL. The antibacterial activity is explained by the phenolics present. LS and CM showed the most significant anti-proliferative activity, which is explained by their zinc content. Conclusion: The most promising plants for further studies are CM, LS and GP.FCT, Fundacao para a Ciencia e a Tecnologia of Portugal [SFRH/BSA/139/2014

Insights into the mechanism of membrane pyrophosphatases by combining experiment and computer simulation

Membrane-integral pyrophosphatases (mPPases) couple the hydrolysis of pyrophosphate (PPi) to the pumping of Na+, H+, or both these ions across a membrane. Recently solved structures of the Na+-pumping Thermotoga maritima mPPase (TmPPase) and H+-pumping Vigna radiata mPPase revealed the basis of ion selectivity between these enzymes and provided evidence for the mechanisms of substrate hydrolysis and ion-pumping. Our atomistic molecular dynamics (MD) simulations of TmPPase demonstrate that loop 5-6 is mobile in the absence of the substrate or substrate-analogue bound to the active site, explaining the lack of electron density for this loop in resting state structures. Furthermore, creating an apo model of TmPPase by removing ligands from the TmPPase: IDP: Na structure in MD simulations resulted in increased dynamics in loop 5-6, which results in this loop moving to uncover the active site, suggesting that interactions between loop 5-6 and the imidodiphosphate and its associated Mg2+ are important for holding a loop-closed conformation. We also provide further evidence for the transport-before-hydrolysis mechanism by showing that the non-hydrolyzable substrate analogue, methylene diphosphonate, induces low levels of proton pumping by VrPPase. (C) 2017 Author(s).Peer reviewe

IMPROvER : the Integral Membrane Protein Stability Selector

Identifying stabilising variants of membrane protein targets is often required for structure determination. Our new computational pipeline, the Integral Membrane Protein Stability Selector (IMPROvER) provides a rational approach to variant selection by employing three independent approaches: deep-sequence, model-based and data-driven. In silico tests using known stability data, and in vitro tests using three membrane protein targets with 7, 11 and 16 transmembrane helices provided measures of success. In vitro, individual approaches alone all identified stabilising variants at a rate better than expected by random selection. Low numbers of overlapping predictions between approaches meant a greater success rate was achieved (fourfold better than random) when approaches were combined and selections restricted to the highest ranked sites. The mix of information IMPROvER uses can be extracted for any helical membrane protein. We have developed the first general-purpose tool for selecting stabilising variants of alpha -helical membrane proteins, increasing efficiency and reducing workload. IMPROvER can be accessed at http://improver.ddns.net/IMPROvER/.Peer reviewe