Nitrogen Management Influences on N Losses to Tile and Surface Water

Nitrogen (N) is a naturally occurring element that is essential to plant growth and crop production. Agriculture has been identified frequently as a major contributor of nitrate-nitrogen to surface water throughout the developed world. Omernik (1977) reported that total N concentrations were nearly nine times greater downstream from agricultural lands than downstream from forested areas with the highest concentrations being found in the Corn Belt States of the Upper Mississippi Basin. Nitrate-N is continually supplied to streams and rivers through mineralization of soil organic matter, particularly where tile drainage has exposed formerly wet soils to oxidation and through the application of fertilizer and animal manures to crop land

Fall vs Spring Application of Nitrogen

Fall application of nitrogen (N) has been a way-of-life for many Upper Midwest farmers for a number of years. The primary advantages of fall compared to spring application are: (1) spread out the work load for both the farmer and dealer, (2) reduce potential soil compaction by eliminating another field operation in the spring, and (3) slight price advantages for fall application. But with the eight-month period between application and the time of greatest N uptake by com, the potential loss of N from the soil system increases. These potential losses lead to poorer economic return and contamination of surface and groundwaters via leaching out of the crop rooting zone

The University of Minnesota Supplemental Fertilizer Nitrogen Worksheet

The University of Minnesota Supplemental Fertilizer N Worksheet was developed as a simple, quick, reliable, and inexpensive alternative decision aid tool to assess potential fertilizer N losses. The worksheet is a series of three questions with multiple-choice answers. Depending on the answers, numbers are assigned for each question that are summed, and then the recommendation is made for supplemental N applications. It has been used with success for 10 years in Minnesota as a decision aid as well as an educational tool regarding fertilizer N management strategies

Nitrogen Management Related to Groundwater Quality in Minnesota

ABSTRACT-Minnesota ranks fifth in overall fertilizer nitrogen (FN) use. Nitrogen is essential for crop production; however, there are concerns about this use relative to profitability and potential impacts on groundwater. There are many sources and sinks of nitrogen in ecosystems. The impact of agricultural management practices on groundwater requires that all sources and fates be considered before FN rates are determined. Research is underway to evaluate relationships between FN application for continuous com and movement of nitrate through soil profiles. Growers making N management decisions have to be able to evaluate the complex nature of N in the environment when they determine their crop needs. Computer software is under development to provide this information on a site specific basis

Hybridization in parasites: consequences for adaptive evolution, pathogenesis and public health in a changing world

[No abstract available

Instant detection of synthetic cannabinoids on physical matrices, implemented on a low-cost, ultraportable device

Synthetic cannabinoids (SCs) make up a class of novel psychoactive substances (NPS), used predominantly in prisons and homeless communities in the U.K. SCs can have severe side effects, including psychosis, stroke, and seizures, with numerous reported deaths associated with their use. The chemical diversity of SCs presents the major challenge to their detection since approaches relying on specific molecular recognition become outdated almost immediately. Ideally one would have a generic approach to detecting SCs in portable settings. The problem of SC detection is more challenging still because the majority of SCs enter the prison estate adsorbed onto physical matrices such as paper, fabric, or herb materials. That is, regardless of the detection modality used, the necessary extraction step reduces the effectiveness and ability to rapidly screen materials on-site. Herein, we demonstrate a truly instant generic test for SCs, tested against real-world drug seizures. The test is based on two advances. First, we identify a spectrally silent region in the emission spectrum of most physical matrices. Second, the finding that background signals (including from autofluorescence) can be accurately predicted is based on tracking the fraction of absorbed light from the irradiation source. Finally, we demonstrate that the intrinsic fluorescence of a large range of physical substrates can be leveraged to track the presence of other drugs of interest, including the most recent iterations of benzodiazepines and opioids. We demonstrate the implementation of our presumptive test in a portable, pocket-sized device that will find immediate utility in prisons and law enforcement agencies around the world

Photochemical fingerprinting Is a sensitive probe for the detection of synthetic cannabinoid receptor agonists; toward robust point-of-care detection

With synthetic cannabinoid receptor agonist (SCRA) use still prevalent across Europe and structurally advanced generations emerging, it is imperative that drug detection methods advance in parallel. SCRAs are a chemically diverse and evolving group, which makes rapid detection challenging. We have previously shown that fluorescence spectral fingerprinting (FSF) has the potential to provide rapid assessment of SCRA presence directly from street material with minimal processing and in saliva. Enhancing the sensitivity and discriminatory ability of this approach has high potential to accelerate the delivery of a point-of-care technology that can be used confidently by a range of stakeholders, from medical to prison staff. We demonstrate that a range of structurally distinct SCRAs are photochemically active and give rise to distinct FSFs after irradiation. To explore this in detail, we have synthesized a model series of compounds which mimic specific structural features of AM-694. Our data show that FSFs are sensitive to chemically conservative changes, with evidence that this relates to shifts in the electronic structure and cross-conjugation. Crucially, we find that the photochemical degradation rate is sensitive to individual structures and gives rise to a specific major product, the mechanism and identification of which we elucidate through density-functional theory (DFT) and time-dependent DFT. We test the potential of our hybrid “photochemical fingerprinting” approach to discriminate SCRAs by demonstrating SCRA detection from a simulated smoking apparatus in saliva. Our study shows the potential of tracking photochemical reactivity via FSFs for enhanced discrimination of SCRAs, with successful integration into a portable device

Differential Gene Expression and Adherence of Escherichia coli O157:H7 In Vitro and in Ligated Pig Intestines

BACKGROUND: Escherichia coli O157:H7 strain 86-24 grown in MacConkey broth (MB) shows almost no adherence to cultured epithelial cells but adheres well in pig ligated intestines. This study investigated the mechanisms associated with the difference between in-vitro and in-vivo adherence of the MB culture. METHODOLOGY/PRINCIPAL FINDINGS: It was found that decreased adherence in vitro by bacteria grown in MB was mainly due to lactose, possibly implicating the involvement of carbon catabolite repression (CCR). Expression of selected virulence-related genes associated with adherence and CCR was then examined by quantitative PCR. When bacteria were grown in MB and Brain Heart Infusion with NaHCO(3) (BHIN) plus lactose, pH was reduced to 5.5-5.9 and there was a significant decrease in expression of the locus of enterocyte effacement (LEE) genes eae, tir, espD, grlA/R and ler, and an increase in cya (cAMP), and two negative regulators of the LEE, gadE and hfq. Putative virulence genes stcE, hlyA, ent and nleA were also decreased in vitro. Reversal of these changes was noted for bacteria recovered from the intestine, where transcripts for qseF and fis and putative virulence factors AidA(15), TerC and Ent/EspL2 were significantly increased, and transcripts for AIDA(48), Iha, UreC, Efa1A, Efa1B, ToxB, EhxA, StcE, NleA and NleB were expressed at high levels. CONCLUSIONS/SIGNIFICANCE: Presence of lactose resulted in decreased expression of LEE genes and the failure of EHEC O157:H7 to adhere to epithelial cells in vitro but this repression was overcome in vivo. CCR and/or acidic pH may have played a role in repression of the LEE genes. Bacterial pathogens need to integrate their nutritional metabolism with expression of virulence genes but little is known of how this is done in E. coli O157:H7. This study indicates one aspect of the subject that should be investigated further