Screening Cage Culture Fish Species for Organic Chlorinated Pesticide and Polychlorinated Biphenyl Residues in Turkey

Organic chlorinated compounds (aldrin, α-endosulfan, β-endosulfan, 2,4’-DDT, and 4,4’-DDE) and polychlorinated biphenyles (PCB28 and PCB52) were screened in fish culture cages off the Aegean Coast of Turkey. Sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) were randomly selected from three coastal areas during June-December 2004. Samples of 114 fish were analyzed by gas chromatography (GC) with an electron capture detector and GC-mass spectrometry (GC-MS). No residues exceeding limits established by the European Union Directive were detected. However, 4,4’-DDE was found in 2.63% of the samples. The amounts of residues in all positive samples were lower than the maximum tolerance limits (0.2-1 mg/kg) accepted by the EU Directive. Contamination levels varied with species, ranging 18-200 ng/g wet weight. Other chemicals were not detected in fish samples

Determination of aldicarb, propoxur, carbofuran, carbaryl and methiocarb residues in honey by HPLC with post-column derivatization and fluorescence detection after elution from a florisil column

The aim of this study was to develop an easy analytical method for determining aldicarb, propoxur, carbofuran, carbaryl and methiocarb residues in honey by high performance liquid chromatography with post-column derivatization and fluorescence detection (HPLC/PCD-FL) for routine analysis. A florisil packed column was used in sample preparation step and showed good performance. The recoveries ranged between 72.02 and 92.02% for various spiking levels (50, 100, 200 ng/g). The limits of detection were 4-5 ng/g, depending on different N-methylcarbamate pesticides. The extraction method used in the present study was easy, inexpensive, and rapid. Thus, it could be used efficiently in the routine monitoring studies

Screening Cage Culture Fish Species for Organic Chlorinated Pesticide and Polychlorinated Biphenyl Residues in Turkey

Organic chlorinated compounds (aldrin, alpha-endosulfan, beta-endosulfan, 2,4'-DDT, and 4,4'-DDE) and polychlorinated biphenyles (PCB28 and PCB52) were screened in fish culture cages off the Aegean Coast of Turkey. Sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) were randomly selected from three coastal areas during June-December 2004. Samples of 114 fish were analyzed by gas chromatography (GC) with an electron capture detector and GC-mass spectrometry (GC-MS). No residues exceeding limits established by the European Union Directive were detected. However, 4,4'-DDE was found in 2.63% of the samples. The amounts of residues in all positive samples were lower than the maximum tolerance limits (0.2-1 mg/kg) accepted by the EU Directive. Contamination levels varied with species, ranging 18-200 ng/g wet weight. Other chemicals were not detected in fish samples

Protective effects of Ankaferd blood stopper on aspirin-induced oxidative mucosal damage in a rat model of gastric injury

The exposure of gastric mucosa to damaging factors, such as ethanol and some therapeutic drugs, produces pathological changes: inflammatory process, hemorrhagic erosions and even acute ulcers. Ankaferd blood stopper (ABS) comprises a standardized mixture of five different plant extracts. The purpose of our present investigations is to explain the participation of reactive oxygen species in acute gastric mucosal damage by acetylsalicylic acid (ASA) and the effects of new hemostatic agent ABS. Experiments were carried out on 23 male Wistar rats. To assess gastric mucosal damage, biochemical and histopathological data were used. The colorimetric assays were used to determine the malondialdehyde (MDA) and superoxide dismutase (SOD) activity. The level of myeloperoxidase (MPO) activity, the level of nitric oxide (NO) and the proinflammatory cytokine tumor necrosis factor- (TNF-) were measured by enzyme-linked immunosorbent assay technique. We demonstrated that the biological effects of ROS were estimated by measuring the tissue and plasma levels of MDA, the products of lipid peroxidation, as well as the activity of SOD and the scavenger of ROS produced by ASA in the experiment group. Moreover, it was found that MPO activity as well as NO and TNF- levels also demonstrated significant improvement by ABS treatment. The pathogenesis of experimental ASA-induced mucosal damage in rat stomach includes the generation of ROS that seems to play an important role, due to the generation of lipid peroxides, accompanied by the impairment of antioxidative enzyme activity of cells. ABS appeared to attenuate the oxidative and inflammatory changes caused by ASA-induced gastric mucosal damage in rats

EGFR mutation status in a series of Turkish non-small cell lung cancer patients.

To access publisher's full text version of this article click on the hyperlink belowEpidermal growth factor receptor (EGFR) mutations are potential markers driving carcinogenesis, and may alter the response to EGFR tyrosine kinase inhibitors in patients with non-small cell lung cancer (NSCLC). The frequency of EGFR mutations in patients with NSCLC differs according to sex, smoking habits and regional-based ethnicity differences. The aim of the present study was to determine the frequency of EGFR mutations in Turkish patients with NSCLC to highlight the importance of regional differences, and their associations with patient characteristics. Genomic DNA was extracted from formalin-fixed and paraffin-embedded tumor tissue sections of 409 NSCLC patients. The most common EGFR mutations in exons 18, 19, 20 and 21 were detected using BioFilmChip-based microarray assay. The overall EGFR mutation frequency was 16.6%, and the highest mutation frequencies were observed in exon 19 (6.4%) and exon 21 (7.3%). There was a higher frequency of EGFR mutations in females compared with males and in never-smokers compared with smokers (both P≤0.05). These results were similar to other European population-based studies, but not consistent Middle-Eastern based studies. The present study may contribute to understanding the gradient frequency of EGFR mutation across different ethnicities, and in designing genome wide-based collaborations that may reveal novel decision making and susceptibility mutations in EGFR in patients with NSCLC.Dokuz Eylul Universit

Autophagy and mTOR pathways in mouse embryonic stem cell, lung cancer and somatic fibroblast cell lines

WOS: 000483551100158PubMed ID: 31148273Embryonic developmental stages and regulations have always been one of the most intriguing aspects of science. Since the cancer stem cell discovery, striking for cancer development and recurrence, embryonic stem cells and control mechanisms, as well as cancer cells and cancer stem cell control mechanisms become important research materials. It is necessary to reveal the similarities and differences between somatic and cancer cells which are formed of embryonic stem cells divisions and determinations. For this purpose, mouse embryonic stem cells (mESCs), mouse skin fibroblast cells (MSFs) and mouse lung squamous cancer cells (SqLCCs) were grown in vitro and the differences between these three cell lines signalling regulations of mechanistic target of rapamycin (mTOR) and autophagic pathways were demonstrated by immunofluorescence and real-time polymerase chain reaction. Expressional differences were clearly shown between embryonic, cancer and somatic cells that mESCs displayed higher expressional level of Atg10, Hdac1 and Cln3 which are related with autophagic regulation and Hsp4, Prkca, Rhoa and ribosomal S6 genes related with mTOR activity. LC3 and mTOR protein levels were lower in mESCs than MSFs. Thus, the mechanisms of embryonic stem cell regulation results in the formation of somatic tissues whereas that these cells may be the causative agents of cancer in any deterioration.Ege UniversitesiEge University [16-TIP-045]; Ege University Scientific Research Projects coordination unitEge University [16-TIP-045