Fish farming through community participation in Assam

The community tanks and ponds in India are seldom used for fish culture. Fish culture as a community based activity.has been shown to be economicallY viable"in some:p~. of the country. This paper describes the success story of fish farmin

Socio-economic dimension of fish farming in Assam

Information on socio-economic framework of the fish farmer community forms a benchmark for policy formulation to develop this economically backward sector. Very few studies have been conducted on the socio-economic aspect of fish farming. Two districts of Assam, Darrang and Nagaon, were selected for this study where 120 respondents from each district were selected randomly. The characteristics representing the personnel and socio-economic attributes of the fish farmers are presented in this paper. The socio-economic status of fish farmers has to be improved by bringing the modern concepts of fish farming to the doorstep of farmers

Techno-economic viability of rice-fish culture in Assam

The economic viability of rice-fish culture practiced by the fanners of Assam in North- Eastern part of India was evaluated using techno-economic approach. The fanners followed extensive level farming practice using low input technology. Cobb-Douglas production function was used to determine the input-output relationship of the system. The cost and return evaluation shows that rice-fish culture can be a more profitable option than monoculture of paddy

Market flow, Price structure and fish marketing system in Assam-A case study

Fish marketing is a flourishing trade in the economy of Assam as it is a preferred food item of 95 % of the state's population. Then: is a wide gap between the present supply and requirement of fish, which is partially met by the supply. To mother fish producing States. The distribution process of flesh fish, dried fish and fish seed has been discussed in this paper. There is a high consumer preference for Indian major carps. Large number of intermediaries are involved in the distribution process of Tesh fish marketing. Women are also involved in the retail trade in the markets and door to door sales in some selected parts of Kamrup District. A well organised marketing network is essential for distribution of fish in the State at reasonable price. The study reveals that the Jagiroad wholesale dried market acts as the nucleus of dried fish trade for the entire North eastern India.. A well organized marketing network is essential for distribution of fish in the State at reasonable price. The present study highlights the prevailing marketing system, market channels, market availability and market-related constraints in the growth of fish marketing in the state

Fisheries economics research and education in India: an overview

The food security problem in India has been alarming due to the rapid growth of population and the reduction of the per capita land. The current scientific, economic, environment and social trends are forcing farmers and policy makers to look for alternatives to fulfill the nutritional requirement for the growing population. Fish with an average of 18- 21 percent protein can be the best alternative in this context. Fisheries sector have been playing an important role in the national economy through improved food supply, employment and income. During 1998-99, fisheries sector contributed Rs.22,223 crores to the total Gross Domestic product (GOP), forming 1.4% of the total

Cell-cultivated aquatic food products: emerging production systems for seafood.

The demand for fish protein continues to increase and currently accounts for 17% of total animal protein consumption by humans. About 90% of marine fish stocks are fished at or above maximum sustainable levels, with aquaculture propagating as one of the fastest growing food sectors to address some of this demand. Cell-cultivated seafood production is an alternative approach to produce nutritionally-complete seafood products to meet the growing demand. This cellular aquaculture approach offers a sustainable, climate resilient and ethical biotechnological approach as an alternative to conventional fishing and fish farming. Additional benefits include reduced antibiotic use and the absence of mercury. Cell-cultivated seafood also provides options for the fortification of fish meat with healthier compositions, such as omega-3 fatty acids and other beneficial nutrients through scaffold, media or cell approaches. This review addresses the biomaterials, production processes, tissue engineering approaches, processing, quality, safety, regulatory, and social aspects of cell-cultivated seafood, encompassing where we are today, as well as the road ahead. The goal is to provide a roadmap for the science and technology required to bring cellular aquaculture forward as a mainstream food source

Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865)

The Puntius denisonii colloquially and more popularly referred to as Miss Kerala is a subtropical fish belonging to the genus Puntius (Barb) and family Cyprinidae. Two cell lines PDF and PDH were developed from the caudal fin and heart of P. denisonii, respectively. The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines. The high growth potential of the cell lines was reflected from the cell doubling time of 28 and 30 h of PDF and PDH cell lines, respectively. The viability of the PDF and PDH cell lines was 70% and 76%, respectively, after 4 mo of storage in liquid nitrogen (−196°C). The origin of the cell lines was confirmed by the amplification of 653 bp fragments of cytochrome oxidase subunit I of mitochondrial DNA genes

DNA barcoding Indian marine fishes

DNA barcoding has been adopted as a global bio-identification system for animals in recent years. A major national programme on DNA barcoding of fish and marine life was initiated in India by the authors during 2006 and 115 species of marine fish covering Carangids, Clupeids, Scombrids, Groupers, Sciaenids, Silverbellies, Mullids, Polynemids and Silurids representing 79 Genera and 37 Families from the Indian Ocean have been barcoded for the first time using cytochrome c oxidase I gene (COI) of the mtDNA. The species were represented by multiple specimens and a total of 397 sequences were generated. After amplification and sequencing of 707 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two parameter (K2P) distances within species, genera, families, orders were 0.30%, 6.60%, 9.91%, 16.00%, respectively. In addition to barcode-based species identification system, phylogenetic relationships among the species have also been attempted. The neighbour-joining tree revealed distinct clusters in concurrence with the taxonomic status of the species

Molecular and Cytogenitic characterization of fish cell lines and its application in aquatic research

Fish cell line has emerged as an important tool in fishery biotechnology. In recent years, various fish cell lines have been developed by different researchers across the country. National Repository on Fish cell lines, established with the aim to preserve fish cell lines for training and education to stakeholders, has started functioning at National Bureau of Fish Genetic Resources, Lucknow. This repository is supposed to characterize and preserve the fish lines developed across the country and serve as a national referral centre for Indian and exotic fish cell lines. Currently, the repository is maintaining 50 fish cell lines deposited by various research institutes in India, including the cell lines developed at cell culture facility of National Bureau of Fish Genetic Resources. The cell lines have been successfully cryopreserved after verifying its authenticity by sequence analysis of two mitochondrial genes, viz.16S rRNA and cytochrome c oxidase sub-unit I. Chromosomal analysis, transfection efficiency and immunocytochemistry are also being used to characterize the cell lines. The facility is serviceable for the collection, deposition and distribution of fish cell lines. This paper discusses the status as well as the methodology adopted for fish cell lines development, characterization and storage at NRFC

Fish Physiology and Biochemistry

Not AvailableDanio rerio, zebrafish, has been widely used as a non-mammalian vertebrate model organism in various studies. The present research describes to develop and characterize a new cell line from a wild strain Indian zebrafish native to Brahmaputra River, Assam, India. The new cell line designated as DRCF was developed from the caudal fin of D. rerio. The cell line was successfully subcultured up to 31 passages. Growth studies revealed that cell growth of DRCF was optimal at 28 ?C in L-15 medium supplemented with 20% FBS. Molecular characterization of the DRCF cell line using mitochondrial genes namely cytochrome oxidase subunit I gene (COI) and 16S rRNA authenticated the true origin of the cell line. The chromosome analysis of the DRCF cell line expressed its 50 diploid chromosome number of D. rerio. The immunocytochemical characterization of the cell line exhibited its fibroblastic morphology. The expression of the green fluorescent protein (GFP) following transfection revealed the suitability of the cell line for transfection studies