The Puntius denisonii colloquially and more
popularly referred to as Miss Kerala is a subtropical fish
belonging to the genus Puntius (Barb) and family Cyprinidae.
Two cell lines PDF and PDH were developed from the caudal
fin and heart of P. denisonii, respectively. The cell lines were
optimally maintained at 26°C in Leibovitz-15 medium
supplemented with 10% fetal bovine serum. A diploid count
of 50 chromosomes at passage 50 was observed in both the
cell lines. The high growth potential of the cell lines was
reflected from the cell doubling time of 28 and 30 h of PDF
and PDH cell lines, respectively. The viability of the PDF and
PDH cell lines was 70% and 76%, respectively, after 4 mo of
storage in liquid nitrogen (−196°C). The origin of the cell
lines was confirmed by the amplification of 653 bp fragments
of cytochrome oxidase subunit I of mitochondrial DNA
genes
