Diet of striped dolphins (Stenella coeruleoalba)in southern Spanish waters

Identifiable food remains were analyzed from 46 stomachs of striped dolphins (Stenella coeruleoalba) stranded in southern Spain between 2007 and 2014. Results suggest that the species feeds mainly on mesopelagic and neritic fish, but also on oceanic squids. Fish species of the family Myctophidae were the main prey in terms of numerical importance and reconstructed prey weight (62% N and 29% W), followed by squids of the family Ommastrephidae (20% W) and bogue (Boops boops) (15% W). The most important prey taxa according to the General Importance Index (GII) were C. maderensis, Ommastrephidae gen. spp., Notoscopelus spp., and M. punctatum. Higher number of mesopelagic myctophids were found in dolphins from the Mediterranean (73% vs. 29% N), while more demersal gobiids and European hake (Merluccius merluccius) were found in those from the Atlantic (44% vs. 1% and 8% vs. <1% N), where a more varied diet was also observed. Differences were also seen in the seasonal importance of some prey, as well as between years, sexes, and maturity states. The diet composition suggests that most of feeding occurred in oceanic regions, during twilight and night hours, while the observed dietary variation may reflect differences in topography, and changes in the prey availability.1,46

High efficiency wireless video networks for next generation of ENG services

This work has been partially funded by the CDTI under the FREEDOM (Servicios de Videocomunicaciones de Alta Eficiencia para Redes Inalámbricas de Nueva Generación en Movilidad) project, with Reference Number IDI20120486.Ruiz, D.; Giménez Gandia, JJ.; Gómez Barquero, D.; Anaya, JJ.; Fernández, FJ.; Valdés Francisco J.; Barjau, C.... (2013). High efficiency wireless video networks for next generation of ENG services. Waves. 5-16. http://hdl.handle.net/10251/55008S51

Ecología trófica del delfín listado en aguas del Mar de Alborán y el Golfo de Cádiz

Existe muy poca información sobre la dieta del delfín listado (Stenella coeruleoalba) en aguas del sur de España. En este estudio se analizaron los estómagos de 61 delfines varados en Andalucía en los últimos ocho años (2007-2014). Diez estómagos estaban vacios y de los 51 individuos restantes, 35 vararon en la costa del Mar de Alborán y 11 en el Golfo de Cádiz. Para las 5 muestras restantes no se dispone de localidad concreta. Los restos de las presas en los estómagos consistieron sobre todo en estructuras duras: otolitos, cristalinos y unos pocos huesos de peces, así como mandíbulas y cristalinos de cefalópodos. No aparecieron otro tipo de restos lo que parece indicar que los individuos no se habían alimentado recientemente. Para caracterizar la dieta se calcularon tres índices (calculados para cada categoría de presa): frecuencia de aparición en los estómagos, número de individuos y peso reconstruido y sus porcentajes respectivos. El delfín listado parece ser una especie predominantemente piscívora (se identificaron restos de 5682 peces frente a solamente 210 cefalópodos) que se alimenta de especies mesopelágicas principalmente. La familia más importante tanto en número como en frecuencia de aparición fue Myctophidae, principalmente Ceratoscopelus maderensis y Myctophum punctatum a las que pertenecieron casi la mitad de los peces identificados (36,4%N, 35,3%F y 12,3%N, 25,5%F, respectivamente). También se identificó Maurolicus muelleri (Sternoptychidae 13,4%N y 17,6%F). Otras familias identificadas presentan una distribución más costera: Sparidae (Boops boops) y Carangidae (Trachurus sp.) (4,5%N, 9,8%F y 1,2%N, 3,9%F, respectivamente). Los cefalópodos identificados pertenecieron a 11 familias siendo la más abundante Ommastrephidae (26,7%N y 39,2%F). Otras familias incluyen representantes oceánicos, Brachioteuthidae (16,7%N y 21,5%F); Chiroteuthidae (6,7%N y 9,8%F); Enoploteuthidae (4,8%N y 3,9%F), Ancistrocheridae (0.5%N y 2%F) y otros de distribución más costera, Sepiolidae (19%N y 19,6%F), Sepiidae (5,7%N y 3,9%F) y Loliginidae (3,8%N y 5,9%F)

Trophic position of dolphins tracks recent changes in the pelagic ecosystem of the Macaronesian region (NE Atlantic)

14 pages, 6 figures, 1 table.-- Open accessDolphins play a key role in marine food webs as predators of mid-trophic-level consumers. Because of their mobility and relatively long life span, they can be used as indicators oflarge-scale changes in the ecosystem. In this study, we calculated the trophic position (TP) of 5 dolphin species from the Canary, Madeira and Azores Islands using bulk and compound-specific stable isotope ratios from muscle tissue to assess trophic adaptations to recent changes in the availability of feeding resources. Dolphin TP values were then compared with those of 7 other species of cetaceans from this region. Analysis of stable nitrogen isotopes in amino acids of the common dolphin indicated non-significant effects of changes in the basal resources of the food web and thus supported the use of bulk samples for TP estimations. Dolphins occupied an intermediate TP (mean: 3.91 to 4.20) between fin (3.25) and sperm whales (4.95). Species-specific TP were equivalent among islands. However, TP increased for the common dolphin and decreased for the bottlenose dolphin (the latter also becoming more oceanic) between 2000 and 2018 in the Canary Islands. These results suggest different impacts of recent changes in the oceanography and in the pelagic food web of the Macaronesian region on the trophic ecology of dolphin speciesThis study was supported in part by the projects QLOCKS (PID2020-115620RB-I00), funded by MCIN/AEI/10.13039/501100011033 (Spain), MISTIC SEAS 2 (‘Applyinga subregional coherent and coordinated approach to the monitoring and assessment of marine biodiversity in Macaronesia for the second cycle of the MSFD’), funded by the Directorate General Environment of the European Commission (Grant Agreement No. 11.0661/2017/750679/SUB/ENV.C2), MISTIC SEAS 3 (‘Developing a coordinated approach for assessing Descriptor 4 via its linkages with D1 and other relevant descriptors in the Macaronesian subregion’), funded by the Directorate General Environment of the European Commission (Grant Agreement No. 110661/2018/794676/SUB/ENV.C2), RACAM (Rede de Arrojamentos de Cetáceos do Arquipélago da Madeira), implemented by the Madeira Whale Museum and funded by the Machico Municipality and projects MARCET (MAC/1.1b/149) and MARCET II (MAC/2.6c/392), both co-financed by EU Programme INTERREG MAC 2014−2020, and through the Commission (28-5307) for ‘Technical scientific advice for the protection of the marine environment: assessment and monitoring of marine strategies, monitoring of marine protected areas of state competence (2018−2021)’ of the Spanish Ministry of Economy and Competitiveness Demographic Challenge (MITECO). Data collection in the Azores was supported by FCT and FRCT through TRACE-PTDC/MAR/74071/2006, MAPCET-M2.1.2/F/012/2011, IF/00943/2013/CP1199/CT0001 (FEDER, COMPETE, QREN, POPH, ESF, Portuguese Ministry for Science and Education, Azores 2020 Operational Programme). M.A.S. was funded by SUMMEREU-H2020 GA 817806. M.A.S. and R.P. were funded by OP AZORES 2020, through the EU Fund 01-0145-FEDER-000140. Okeanos is funded by FCT (UIDB/05634/2020) and by the Regional Government of the Azores (M1.1.A/REEQ.CIENTÍFICO UI&D/2021/010). J.G. was supported by the Spanish National Programme Juan de la Cierva-Formación (MCIN/AEI/10.13039/501100011033 FJC2019-040016-I). This work acknowledges the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) to the Institute of Marine Science (ICM-CSIC)Peer reviewe

Multiple myeloma and SARS-CoV-2 infection: clinical characteristics and prognostic factors of inpatient mortality

There is limited information on the characteristics, prognostic factors, and outcomes of patients with multiplemyeloma (MM) hospitalized with COVID-19. This retrospective case series investigated 167 patients reported from 73hospitals within the Spanish Myeloma Collaborative Group network in March and April, 2020. Outcomes werecompared with 167 randomly selected, contemporary, age-/sex-matched noncancer patients with COVID-19 admittedat six participating hospitals. Among MM and noncancer patients, median age was 71 years, and 57% of patients weremale; 75 and 77% of patients, respectively, had at least one comorbidity. COVID-19 clinical severity wasmoderate-severe in 77 and 89% of patients and critical in 8 and 4%, respectively. Supplemental oxygen was requiredby 47 and 55% of MM and noncancer patients, respectively, and 21%/9% vs 8%/6% required noninvasive/invasiveventilation. Inpatient mortality was 34 and 23% in MM and noncancer patients, respectively. Among MM patients,inpatient mortality was 41% in males, 42% in patients aged >65 years, 49% in patients with active/progressive MM athospitalization, and 59% in patients with comorbid renal disease at hospitalization, which were independentprognostic factors on adjusted multivariate analysis. This case series demonstrates the increased risk and identifiespredictors of inpatient mortality among MM patients hospitalized with COVID-19

Early-Career Coordinated Distributed Experiments: Empowerment Through Collaboration

Este artículo contiene 7 páginas, 1 tabla, 3 figuras.Coordinated distributed experiments (CDEs) enable the study of large-scale ecological patterns in geographically dispersed areas, while simultaneously providing broad academic and personal benefits for the participants. However, the effective involvement of early-career researchers (ECRs) presents major challenges. Here, we analyze the benefits and challenges of the first CDE exclusively led and conducted by ECRs (i.e. ECR-CDE), which sets a baseline for similar CDEs, and we provide recommendations for successful CDE execution. ECR-CDEs achieve most of the outcomes identified in conventional CDEs as well as extensive benefits for the young cohort of researchers, including: (i) receiving scientific credit, (ii) peer-training in new concepts and methods, (iii) developing leadership and communication skills, (iv) promoting a peer network among ECRs, and (v) building on individual engagement and independence. We also discuss the challenges of ECR-CDEs, which are mainly derived from the lack of independence and instability of the participants, and we suggest mechanisms to address them, such as resource re-allocation and communication strategies. We conclude that ECR-CDEs can be a relevant tool to empower ECRs across disciplines by fostering their training, networking and personal well-being.The authors were supported by the following founding: NC the support of the Beatriu de Pinós postdoctoral program of the Government of Catalonia’s Secretariat for Universities and Research of the Ministry of Economy and Knowledge (BP2016- 00215), EE by a predoctoral grant from the Basque Government (2014-2017), AB by a Generalitat de Catalunya—Beatriu de Pinós (BP-00385-2016), AMG-F by a predoctoral research grant (BES-2013-065770) from the Spanish Ministry of Economy and Competitiveness, MAr by a postdoctoral grant from the Basque Government, MIA by a Juan de la Cierva postdoctoral grant (FJCI-2015-26192), PR-L by a Margalida Comas postdoctoral contract (PD/031/2018) funded by the Government of the Balearic Islands and the European Social Fund, AP by a Ramón Areces Foundation Postdoctoral Scholarship, and AL by a Kempe Foundation stipend. DOMIPEX project was founded by the First Call of Collaborative Projects among Young Researchers of the Iberian Association of Limnology (AIL; 2013-2015).Peer reviewe

New insights on the critically endangered population of bottlenose dolphins from Sado estuary (Portugal)

34th European Cetacean Society Conference, O Grove, 16-20 April 2023Identifying threatened populations and quantifying their vulnerability is crucial for establishing priorities for conservation and providing robust information for decision-making. Small population size is a fundamental factor increasing the risk of extinction, owing to demographic and genetic stochasticity. When populations become too small, additional threats to stability and persistence arise, which can exacerbate the difficulty of stopping or reversing the decline. The bottlenose dolphin (Tursiops truncatus) population inhabiting the Sado estuary region (Portugal) is one of the smallest resident populations of this species in Europe. Dedicated research over the last four decades has revealed year-round long-term site fidelity to the estuary, very low levels of immigration, high calf/juvenile mortality, and an ageing, declining population. In this collaborative study, we update previous information on population dynamics and add important information about the genetic diversity (microsatellites, mtDNA, SNPs markers), inbreeding levels, population structure, and persistent organic pollutants (PCBs, DDTs, PBDEs, and HBCDs) loads. Presently, the Sado population has 25 individuals (10M; 8F; 7 Unknown sex), of which six individuals are more than 40 years old. We found lower genetic diversity, presence of unique maternal lineages, and high levels of relatedness (r˃0.412). These animals are genetically differentiated from the other bottlenose dolphins sampled in the area (Iberian Peninsula, Azores and Madeira) suggesting an isolated population. For PCBs, five (62%) animals had concentrations higher than the highest threshold for PCB toxicology (˃41mg/kg lipid weight, can cause immunosuppression and/or reproductive impairment) including one calf less than 6 months old. This, together with the fact that this population inhabits one of the most polluted estuaries in Portugal, characterized by multiple sources of contamination and increasing anthropogenic pressures (e.g. dredging, boat traffic, and dolphin-watching activities) highlights the critical situation of this resident bottlenose dolphin population and the urgency to implement stringent and effective conservation measuresN

Integrin-Specific Mechanoresponses to Compression and Extension Probed by Cylindrical Flat-Ended AFM Tips in Lung Cells

Cells from lung and other tissues are subjected to forces of opposing directions that are largely transmitted through integrin-mediated adhesions. How cells respond to force bidirectionality remains ill defined. To address this question, we nanofabricated flat-ended cylindrical Atomic Force Microscopy (AFM) tips with ∼1 µm2 cross-section area. Tips were uncoated or coated with either integrin-specific (RGD) or non-specific (RGE/BSA) molecules, brought into contact with lung epithelial cells or fibroblasts for 30 s to form focal adhesion precursors, and used to probe cell resistance to deformation in compression and extension. We found that cell resistance to compression was globally higher than to extension regardless of the tip coating. In contrast, both tip-cell adhesion strength and resistance to compression and extension were the highest when probed at integrin-specific adhesions. These integrin-specific mechanoresponses required an intact actin cytoskeleton, and were dependent on tyrosine phosphatases and Ca2+ signaling. Cell asymmetric mechanoresponse to compression and extension remained after 5 minutes of tip-cell adhesion, revealing that asymmetric resistance to force directionality is an intrinsic property of lung cells, as in most soft tissues. Our findings provide new insights on how lung cells probe the mechanochemical properties of the microenvironment, an important process for migration, repair and tissue homeostasis

Fine scale population genomics of the bottlenose dolphin off Western Iberia (northeast Atlantic)

34th European Cetacean Society Conference, O Grove, 16-20 April 2023Bottlenose dolphin (Tursiops truncatus) populations sampled across various geographic regions show fine-scale patterns of genetic structure, likely associated with local habitat preferences. Several population genetic studies have focused on populations from the northeast Atlantic Ocean (NEA), very few including the Portuguese coast. This study investigated the population structure and demographic history of the bottlenose dolphin in this region using double digest restriction site associated DNA sequencing (ddRAD-seq). Samples were collected from the Iberian Peninsula including SW Spain, the Portuguese coast (including Sado estuary), NW Spain and from two Portuguese Macaronesian archipelagos, Madeira and Azores (N=110 samples). We genotyped thousands of single nucleotide polymorphisms markers to study the fine-scale population structure, inbreeding levels and demographic history. Bayesian clustering and Principal Component Analyses showed three major genetic clusters: Pelagic (incl. Madeira and Azores; North Galicia, Portugal coast), Southern/Mediterranean (incl. Gibraltar/Cadiz, previously identified as of Mediterranean origin, and Portugal coast), and Resident (Sado and south Galicia). These clusters differ from each other in terms of genetic diversity and inbreeding levels. While the Pelagic and Southern/Med clusters shows high genetic diversity and a wide geographic distribution, the Resident populations show the opposite. The Resident cluster can be subdivided in Galicia and Sado populations, the latter revealing concerning levels of inbreeding. Preliminary demographic analyses suggest that the Resident populations diverged from the others prior to the Southern/Med-Pelagic divergence, although further analyses are needed. Our results suggest that i) the Resident populations of the Iberian Peninsula should be considered as different management units; ii) bottlenose dolphins sampled along the Portuguese coast are highly genetically diverse, including individuals from the three different clusters; and iii) the Sado population faces a critical situation. Within the framework of the recently proposed SACs for bottlenose dolphins in Portugal, these results highlight the importance of discussions on conservation and management policiesN

Recent updates and developments to plant genome size databases

Two plant genome size databases have been recently updated and/or extended: the Plant DNA C-values database (http://data.kew.org/cvalues), and GSAD, the Genome Size in Asteraceae database (http://www.asteraceaegenomesize.com). While the first provides information on nuclear DNA contents across land plants and some algal groups, the second is focused on one of the largest and most economically important angiosperm families, Asteraceae. Genome size data have numerous applications: they can be used in comparative studies on genome evolution, or as a tool to appraise the cost of whole-genome sequencing programs. The growing interest in genome size and increasing rate of data accumulation has necessitated the continued update of these databases. Currently, the Plant DNA C-values database (Release 6.0, Dec. 2012) contains data for 8510 species, while GSAD has 1219 species (Release 2.0, June 2013), representing increases of 17 and 51%, respectively, in the number of species with genome size data, compared with previous releases. Here we provide overviews of the most recent releases of each database, and outline new features of GSAD. The latter include (i) a tool to visually compare genome size data between species, (ii) the option to export data and (iii) a webpage containing information about flow cytometry protocols