143 research outputs found
Biological approaches for addressing the grand challenge of providing access to clean drinking water
The U.S. National Academy of Engineering (NAE) recently published a document presenting "Grand Challenges for Engineering". This list was proposed by leading engineers and scientists from around the world at the request of the U.S. National Science Foundation (NSF). Fourteen topics were selected for these grand challenges, and at least seven can be addressed using the tools and methods of biological engineering. Here we describe how biological engineers can address the challenge of providing access to clean drinking water. This issue must be addressed in part by removing or inactivating microbial and chemical contaminants in order to properly deliver water safe for human consumption. Despite many advances in technologies this challenge is expanding due to increased pressure on fresh water supplies and to new opportunities for growth of potentially pathogenic organisms
Impact of an Alcohol-Based Hand Sanitizer Intervention on the Spread of Viruses in Homes
The objectives of this study were to determine the movement of a virus throughout a household and the impact of an alcohol-based hand sanitizer (ABHS) on reducing the movement and exposure of the virus to household members. Bacterial virus MS-2 was used as the surrogate for human enteric and respiratory viruses. Seven households with families having at least two children in the age range of 2â18 living in the home were used in this study. The hands of one adult family member were contaminated with 1 Ă 10(8). MS-2 bacteriophage in each home. After 8Â h, the hands of each family member (10 fingers) and 20 frequently touched fomites were sampled to determine baseline contamination without intervention. Within 8Â h, MS-2 was detected on all of the family memberâs hands and most of the fomites. The intervention consisted of providing the families in all selected homes with bottles of an ABHS, which were placed in the kitchen, bathrooms, and nurseries. Smaller individual bottles were provided for each family member greater than 12Â years old to place in purses, pockets, backpacks, etc. The families were instructed to use the ABHS one time or three times during the day. For one and three uses, a statistically significant reduction of virus on un-inoculated and inoculated hands of ~99Â % occurred within 8Â h. Similar reductions occurred on fomites throughout the households (97â99Â %). These results demonstrate that the use of an ABHS can significantly reduce transfer of a virus to the hands, and to the commonly touched surfaces within the household
Viruses and Drug Resistant Bacteria in Groundwater of Southeastern Minnesota
ABSTRACT-Karst topography of soils in southeastern Minnesota is responsible for the formation of sinkholes, subsurface cracks, and underground rivers which may enhance the transportation of surface contaminants into groundwater. The present study was conducted to determine the presence of human pathogenic viruses, coliforms, fecal coliforms and coliphages in private rural wells of this area. The occurrence of drug resistance in bacteria isolated from groundwater also was studied. Coliform bacteria were detected at least once from 22 of the 26 sites sampled over 34 months. Water from 10 sites yielded drug-resistant indicator bacteria; 25 of 38 (65.8 percent) total coliforms and 9 of27 (33.3 percent) fecal coliforms tested were found to carry drug resistance. Human enteric viruses were detected by DNA hybridization and/or virus isolation techniques in nine samples from seven different sites, some in the absence of fecal coliforms. Of the 161 samples tested for coliphages, 13 samples from seven sites were found positive. On two occasions, coliphages were isolated from samples in which coliforms were absent. These findings indicate that potential public health problems exist in this region
Quantification and Genetic Analysis of Salivirus/Klassevirus in Wastewater in Arizona, USA
Salivirus/klassevirus sequences were identified in 7 (15Â %) wastewater samples collected in Arizona monthly for a year, with the highest concentration of 2.28Â ĂÂ 105 and 2.46Â ĂÂ 104 copies/L in influent and effluent, respectively. This is the first report of quantification and genetic analysis of salivirus/klassevirus in water samples in the United States.International Fulbright Science & Technology AwardJapan Society for the Promotion of Science (Postdoctoral Fellowship for Research Abroad (FY 2011 no. 517)))National Science Foundation (U.S.) (Water and Environmental Technology (WET) Center, University of Arizona
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Reduction of Arcobacter at Two Conventional Wastewater Treatment Plants in Southern Arizona, USA
This study aimed to identify the bacterial community in two wastewater treatment plants (WWTPs) and to determine the occurrence and reduction of Arcobacter, along with virulence genes (ciaB and pldA). A total of 48 samples (24 influent and 24 effluent) were collected at two WWTPs in southern Arizona in the United States, monthly from August 2011 to July 2012. Bacterial DNA extract was utilized for 16S rRNA metagenomic sequencing. Quantification of Arcobacter 16S rRNA gene was conducted using a recently developed SYBR Green-based quantitative PCR assay. Among 847 genera identified, 113 (13%) were identified as potentially pathogenic bacteria. Arcobacter 16S rRNA gene was detected in all influent samples and ten (83%) and nine (75%) effluent samples at each plant, respectively. Log reduction ratios of Arcobacter 16S rRNA gene in Plant A and Plant B were 1.7 Âą 0.9 (n = 10) and 2.3 Âą 1.5 (n = 9), respectively. The ciaB gene was detected by quantitative PCR in eleven (92%) and twelve (100%) of 12 influent samples from Plant A and Plant B, respectively, while the pldA gene was detected in eight (67%) and six (50%) influent samples from Plant A and Plant B, respectively. The prevalence of potentially pathogenic bacteria in WWTP effluent indicated the need for disinfection before discharge into the environment.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
Combating SARS-CoV-2: Leveraging microbicidal experiences with other emerging/re-emerging viruses
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Wuhan City, China, late in December 2019 is another example of an emerging zoonotic virus that threatens public health and international travel and commerce. When such a virus emerges, there is often insufficient specific information available on mechanisms of virus dissemination from animal to human or from person to person, on the level or route of infection transmissibility or of viral release in body secretions/excretions, and on the survival of virus in aerosols or on surfaces. The effectiveness of available virucidal agents and hygiene practices as interventions for disrupting the spread of infection and the associated diseases may not be clear for the emergent virus. In the present review, we recommend approaches for infection prevention and control for SARS-CoV-2 which can be invoked based on pre-existing data on microbicidal and hygiene effectiveness for related and unrelated enveloped viruse
Occurrence and genetic diversity of human cosavirus in influent and effluent of wastewater treatment plants in Arizona, United States
Human cosavirus (HCoSV) is a novel member of the family Picornaviridae. We investigated the prevalence and genetic diversity of HCoSV in influent and effluent wastewater in Arizona over a 12-month period, from August 2011 to July 2012. HCoSV sequences were identified in six (25 %) influent samples and one (4 %) effluent sample, with the highest concentration of 3.24 x 10[superscript 5] and 1.54 x 10Âł copies/liter in influent and effluent, respectively. The strains were characterized based on their 5â untranslated region and classified into species A and D, demonstrating that genetically heterogeneous HCoSV were circulating with a clear temporal shift of predominant strains in the study area.National Science Foundation (U.S.) Water and Environmental Technology (WET) CenterJapan Society for the Promotion of Science. Postdoctoral Fellowship for Research Abroad (FY 2011 no. 517
Reducing antibiotic prescribing and addressing the global problem of antibiotic resistance by targeted hygiene in the home and everyday life settings: A position paper
Antimicrobial resistance (AMR) continues to threaten global health. Although global and national AMR action plans are in place, infection prevention and control is primarily discussed in the context of health care facilities with home and everyday life settings barely addressed. As seen with the recent global SARS-CoV-2 pandemic, everyday hygiene measures can play an important role in containing the threat from infectious microorganisms. This position paper has been developed following a meeting of global experts in London, 2019. It presents evidence that home and community settings are important for infection transmission and also the acquisition and spread of AMR. It also demonstrates that the targeted hygiene approach offers a framework for maximizing protection against colonization and infections, thereby reducing antibiotic prescribing and minimizing selection pressure for the development of antibiotic resistance. If combined with the provision of clean water and sanitation, targeted hygiene can reduce the circulation of resistant bacteria in homes and communities, regardless of a country\u27s Human Development Index (overall social and economic development). Achieving a reduction of AMR strains in health care settings requires a mirrored reduction in the community. The authors call upon national and international policy makers, health agencies, and health care professionals to further recognize the importance of targeted hygiene in the home and everyday life settings for preventing and controlling infection, in a unified quest to tackle AMR
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Modeling the role of fomites in a norovirus outbreak
Norovirus accounts for a large portion of the gastroenteritis disease burden, and outbreaks have occurred in a wide variety of environments. Understanding the role of fomites in norovirus transmission will inform behavioral interventions, such as hand washing and surface disinfection. The purpose of this study was to estimate the contribution of fomite-mediated exposures to infection and illness risks in outbreaks. A simulation model in discrete time that accounted for hand-to-porous surfaces, hand-to-nonporous surfaces, hand-to-mouth, -eyes, -nose, and hand washing events was used to predict 17âhr of simulated human behavior. Norovirus concentrations originated from monitoring contamination levels on surfaces during an outbreak on houseboats. To predict infection risk, two dose-response models (fractional Poisson and 2F1 hypergeometric) were used to capture a range of infection risks. A triangular distribution describing the conditional probability of illness given an infection was multiplied by modeled infection risks to estimate illness risks. Infection risks ranged from 70.22% to 72.20% and illness risks ranged from 21.29% to 70.36%. A sensitivity analysis revealed that the number of hand-to-mouth contacts and the number of hand washing events had strong relationships with model-predicted doses. Predicted illness risks overlapped with leisure setting and environmental attack rates reported in the literature. In the outbreak associated with the viral concentrations used in this study, attack rates ranged from 50% to 86%. This model suggests that fomites may have accounted for 25% to 82% of illnesses in this outbreak. Fomite-mediated exposures may contribute to a large portion of total attack rates in outbreaks involving multiple transmission modes. The findings of this study reinforce the importance of frequent fomite cleaning and hand washing, especially when ill persons are present.12 month embargo; published online: 04 Feb 2019This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
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